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The map label for this gene is guaB1 [C]

Identifier: 221230592

GI number: 221230592

Start: 2453625

End: 2455061

Strand: Direct

Name: guaB1 [C]

Synonym: MLBr_02066

Alternate gene names: 221230592

Gene position: 2453625-2455061 (Clockwise)

Preceding gene: 221230591

Following gene: 221230593

Centisome position: 75.08

GC content: 62.42

Gene sequence:

>1437_bases
ATGAGGTTTCTGGACGGGCACCAGCCCAGGTTCGACCTAACCTATAAAGACGTTTTCATCGTGCCACACCGGTCGGATGT
TGCGTCGCGATTTGACGTCGATTTGTCAACCTATGACGGCTCCGGCACCACTATTCCCGTAGTGGTCGCCAACATGACCA
CGGTGGCCGGGCAACGGATGGCAGAGACGGTAGCTCGCCGGGGCGGCTTAGTAATTTTACCGCAGGATCTGCCGATTGCA
ACAGTGCAGCAGGCAGTGGAGTTCGTCAAGAGCCGTGACCTGGTTATCGATACCCCGGTGTTACTGACACCTAACGATTC
GGTGTCCGACGCGACCACACTGATCCACAAGCGCGCGCACGGTGTTGCGGTGGTGGCCTTCGAAGGCCGCCCAATTGGTC
TGGTGCGCGAATCGTCTTGCCTAGGTGTGGACCGCTTCACTCGGGTGCGGGATGTCGCGACTACCGACTTTGTAACCGCC
CCGGTGGGCACCGATCCACGCAAGATCTTCGACCTGCTCGAACATGCCCCGATCGACGTCGCGGTGCTGACTAACGCCGA
CGGCACGCTGGCCGGAGTACTGACCCGCACCGGGGCGATCCGCGCCGGCATCTACACCCCAGCCACCGATGCCCGCGGAC
GGCTCCGCATCGGTGCTGCTGTCGGCATAAACGGCGATGTAGCCGGCAAGGCCCAGTCCCTCGCCGAGGCAGGTGTCGAT
GTGCTGGTCGTCGACACCGCACACGGATACCAAGTCAAGACACTCGAGGCGATCAAATGCGTCGCATCGCTGAACTTGGG
TGTGCCGTTGGTCGCGGGCAACGTGGTGTCGGCTGAAGGTACTCGCGAGCTACTCAACGCCGGGGCGACCATCGTCAAGG
TCGGCGTCGGCCCAGGAGCAATGTGCACAACTCGGATGATGACCGGTGTTGGCCGACCGCAATTCTCAGCTGTACTCGAA
TGTGCCTCTGCTGCAAGGAAACTCAATAGGCACGTGTGGGCTGATGGAGGAGTCCGCCATCCGCGTGACGTGGCGCTGGC
GTTGGCCGCAGGAGCATCGAACGTGATGATCGGGTCGTGGTTCGCCGGGACTTATGAGTCACCCGGGGACCTCATGCGGG
ACCGCCACGACCAACCGTACAAGGAAAGCTACGGCATGGCGTCCAAGCGAGCGGTGGTTGCCCGCACCGTTGCCGACAGC
TCATTCGATCGCGCTCGCAAGGCGTTATTCGATGAAGGCATCTCGACCTCACGGATGGGGCTGGATCCCGACCACGGTGG
TGTCGAGGACCTGATCGATCACATCACCTCCGGCGTGCGCAGCACCTGCACCTATGTCGGCGCGTCGACCCTGGCGGAGC
TGCATGAGAAGGCTGTCGTCGGGGTGCAGTCAGCGGCGGGTTTCGCCGAGGGCCATCCACTGCCCTCGGGGTGGTGA

Upstream 100 bases:

>100_bases
GAGTGAAGACCGTAGCGAAGTTACGGTCTAGTTGGCCAAGCTAAGAGACATCCGGTGGCCCCACACTAAATACAAAATCT
TTTAGAGTGAAACGAGAGCG

Downstream 100 bases:

>100_bases
CACCTGACCTCGGCGAAAGTCGCTACGATGTGAACTTCTGGATTTCAGGGCACACAGCAATGTGCGCTGCTCCGTGGCCC
AAACCGTTGAAGAGCGAAAG

Product: inosine 5-monophosphate dehydrogenase

Products: xanthosine 5'-phosphate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRFLDGHQPRFDLTYKDVFIVPHRSDVASRFDVDLSTYDGSGTTIPVVVANMTTVAGQRMAETVARRGGLVILPQDLPIA
TVQQAVEFVKSRDLVIDTPVLLTPNDSVSDATTLIHKRAHGVAVVAFEGRPIGLVRESSCLGVDRFTRVRDVATTDFVTA
PVGTDPRKIFDLLEHAPIDVAVLTNADGTLAGVLTRTGAIRAGIYTPATDARGRLRIGAAVGINGDVAGKAQSLAEAGVD
VLVVDTAHGYQVKTLEAIKCVASLNLGVPLVAGNVVSAEGTRELLNAGATIVKVGVGPGAMCTTRMMTGVGRPQFSAVLE
CASAARKLNRHVWADGGVRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRHDQPYKESYGMASKRAVVARTVADS
SFDRARKALFDEGISTSRMGLDPDHGGVEDLIDHITSGVRSTCTYVGASTLAELHEKAVVGVQSAAGFAEGHPLPSGW

Sequences:

>Translated_478_residues
MRFLDGHQPRFDLTYKDVFIVPHRSDVASRFDVDLSTYDGSGTTIPVVVANMTTVAGQRMAETVARRGGLVILPQDLPIA
TVQQAVEFVKSRDLVIDTPVLLTPNDSVSDATTLIHKRAHGVAVVAFEGRPIGLVRESSCLGVDRFTRVRDVATTDFVTA
PVGTDPRKIFDLLEHAPIDVAVLTNADGTLAGVLTRTGAIRAGIYTPATDARGRLRIGAAVGINGDVAGKAQSLAEAGVD
VLVVDTAHGYQVKTLEAIKCVASLNLGVPLVAGNVVSAEGTRELLNAGATIVKVGVGPGAMCTTRMMTGVGRPQFSAVLE
CASAARKLNRHVWADGGVRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRHDQPYKESYGMASKRAVVARTVADS
SFDRARKALFDEGISTSRMGLDPDHGGVEDLIDHITSGVRSTCTYVGASTLAELHEKAVVGVQSAAGFAEGHPLPSGW
>Mature_478_residues
MRFLDGHQPRFDLTYKDVFIVPHRSDVASRFDVDLSTYDGSGTTIPVVVANMTTVAGQRMAETVARRGGLVILPQDLPIA
TVQQAVEFVKSRDLVIDTPVLLTPNDSVSDATTLIHKRAHGVAVVAFEGRPIGLVRESSCLGVDRFTRVRDVATTDFVTA
PVGTDPRKIFDLLEHAPIDVAVLTNADGTLAGVLTRTGAIRAGIYTPATDARGRLRIGAAVGINGDVAGKAQSLAEAGVD
VLVVDTAHGYQVKTLEAIKCVASLNLGVPLVAGNVVSAEGTRELLNAGATIVKVGVGPGAMCTTRMMTGVGRPQFSAVLE
CASAARKLNRHVWADGGVRHPRDVALALAAGASNVMIGSWFAGTYESPGDLMRDRHDQPYKESYGMASKRAVVARTVADS
SFDRARKALFDEGISTSRMGLDPDHGGVEDLIDHITSGVRSTCTYVGASTLAELHEKAVVGVQSAAGFAEGHPLPSGW

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains

Homologues:

Organism=Homo sapiens, GI66933016, Length=463, Percent_Identity=28.7257019438445, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI50541956, Length=219, Percent_Identity=43.8356164383562, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI50541954, Length=216, Percent_Identity=44.4444444444444, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI50541952, Length=216, Percent_Identity=44.4444444444444, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI50541948, Length=216, Percent_Identity=44.4444444444444, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI156104880, Length=231, Percent_Identity=41.1255411255411, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI217035146, Length=463, Percent_Identity=29.1576673866091, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI34328928, Length=463, Percent_Identity=29.1576673866091, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI156616279, Length=463, Percent_Identity=29.1576673866091, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI34328930, Length=463, Percent_Identity=29.1576673866091, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI217035148, Length=462, Percent_Identity=29.6536796536797, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI217035152, Length=459, Percent_Identity=28.7581699346405, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI217035150, Length=462, Percent_Identity=28.5714285714286, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788855, Length=470, Percent_Identity=32.9787234042553, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786293, Length=222, Percent_Identity=40.5405405405405, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17560440, Length=220, Percent_Identity=41.8181818181818, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71994385, Length=485, Percent_Identity=27.6288659793814, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71994389, Length=422, Percent_Identity=28.436018957346, Blast_Score=122, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322012, Length=468, Percent_Identity=27.7777777777778, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323464, Length=476, Percent_Identity=26.890756302521, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323585, Length=470, Percent_Identity=27.8723404255319, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319352, Length=373, Percent_Identity=27.0777479892761, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24641071, Length=469, Percent_Identity=28.7846481876333, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24641073, Length=469, Percent_Identity=28.7846481876333, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28571163, Length=428, Percent_Identity=28.2710280373832, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): Y2066_MYCLE (O32912)

Other databases:

- EMBL:   AL008609
- EMBL:   AL583924
- PIR:   T44751
- RefSeq:   NP_302378.1
- ProteinModelPortal:   O32912
- SMR:   O32912
- EnsemblBacteria:   EBMYCT00000029402
- GeneID:   908961
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2066
- NMPDR:   fig|272631.1.peg.1250
- Leproma:   ML2066
- GeneTree:   EBGT00050000017422
- HOGENOM:   HBG298985
- OMA:   ESFGMAS
- ProtClustDB:   PRK07807
- BioCyc:   MLEP272631:ML2066-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR018529
- InterPro:   IPR001093
- InterPro:   IPR005991
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000130
- TIGRFAMs:   TIGR01303

Pfam domain/function: PF00571 CBS; PF00478 IMPDH

EC number: 1.1.1.205

Molecular weight: Translated: 50384; Mature: 50384

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS51371 CBS

Important sites: ACT_SITE 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLDGHQPRFDLTYKDVFIVPHRSDVASRFDVDLSTYDGSGTTIPVVVANMTTVAGQRM
CCCCCCCCCCEEEEEEEEEEEECCCCHHHHHCCEEEEECCCCCEEEEEEECHHHHHHHHH
AETVARRGGLVILPQDLPIATVQQAVEFVKSRDLVIDTPVLLTPNDSVSDATTLIHKRAH
HHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHCC
GVAVVAFEGRPIGLVRESSCLGVDRFTRVRDVATTDFVTAPVGTDPRKIFDLLEHAPIDV
CEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCE
AVLTNADGTLAGVLTRTGAIRAGIYTPATDARGRLRIGAAVGINGDVAGKAQSLAEAGVD
EEEECCCCCEEHHHHHCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCC
VLVVDTAHGYQVKTLEAIKCVASLNLGVPLVAGNVVSAEGTRELLNAGATIVKVGVGPGA
EEEEECCCCCEEHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHCCCEEEEEECCCCH
MCTTRMMTGVGRPQFSAVLECASAARKLNRHVWADGGVRHPRDVALALAAGASNVMIGSW
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHEEEEECCCCCEEEEEE
FAGTYESPGDLMRDRHDQPYKESYGMASKRAVVARTVADSSFDRARKALFDEGISTSRMG
ECCCCCCCHHHHHCCCCCCHHHHCCCCCCHHHEEHHHHCCHHHHHHHHHHHCCCCCCCCC
LDPDHGGVEDLIDHITSGVRSTCTYVGASTLAELHEKAVVGVQSAAGFAEGHPLPSGW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRFLDGHQPRFDLTYKDVFIVPHRSDVASRFDVDLSTYDGSGTTIPVVVANMTTVAGQRM
CCCCCCCCCCEEEEEEEEEEEECCCCHHHHHCCEEEEECCCCCEEEEEEECHHHHHHHHH
AETVARRGGLVILPQDLPIATVQQAVEFVKSRDLVIDTPVLLTPNDSVSDATTLIHKRAH
HHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHCC
GVAVVAFEGRPIGLVRESSCLGVDRFTRVRDVATTDFVTAPVGTDPRKIFDLLEHAPIDV
CEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCE
AVLTNADGTLAGVLTRTGAIRAGIYTPATDARGRLRIGAAVGINGDVAGKAQSLAEAGVD
EEEECCCCCEEHHHHHCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCC
VLVVDTAHGYQVKTLEAIKCVASLNLGVPLVAGNVVSAEGTRELLNAGATIVKVGVGPGA
EEEEECCCCCEEHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHCCCEEEEEECCCCH
MCTTRMMTGVGRPQFSAVLECASAARKLNRHVWADGGVRHPRDVALALAAGASNVMIGSW
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHEEEEECCCCCEEEEEE
FAGTYESPGDLMRDRHDQPYKESYGMASKRAVVARTVADSSFDRARKALFDEGISTSRMG
ECCCCCCCHHHHHCCCCCCHHHHCCCCCCHHHEEHHHHCCHHHHHHHHHHHCCCCCCCCC
LDPDHGGVEDLIDHITSGVRSTCTYVGASTLAELHEKAVVGVQSAAGFAEGHPLPSGW
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: inosine 5'-phosphate; NAD+; H2O

Specific reaction: inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002