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The map label for this gene is truA [H]

Identifier: 221230534

GI number: 221230534

Start: 2343254

End: 2344003

Strand: Reverse

Name: truA [H]

Synonym: MLBr_01955

Alternate gene names: 221230534

Gene position: 2344003-2343254 (Counterclockwise)

Preceding gene: 221230535

Following gene: 221230532

Centisome position: 71.72

GC content: 58.27

Gene sequence:

>750_bases
GTGCTGGATGAAGCTTTCACGACTGTTTTCCGCACACCGGTGCGACTGCGCGCTGCCGGGCGCACCGACACAGGCGTACA
CGCCACCGGGCAGGTGGCCCATGTAGATATTCCCGGAACTGCCTTGTCGAAAGCCAATCCACGCTCACCACATACCGGTG
ATCCGGAATTCCTGCCTCTGCTGCGGCGTCTCAGCCGGCTCCTGCCTGTTGACGTCCGAGTTCTCGATATAGCCTGTGCA
CCAGTAGGATTCGATGCCAGGTTTTCAGCCTTGCGGCGGCATTATCGGTATCGGTTATCGACGGCACCCTACGGAGTTCA
GCCGAACCAGGCGCGCTACGTCACTGCTTGGCCTCGTGAACTGGATCTGGATTCTATGTCCGCTGCATCGCGAGATTTGT
TGGGGCTACGTGACTTTGCGGTGTTTTGCCGTCATCGCCACGGTGCTACCACCATTCGTGAGTTACAGCGGCTGGAATGG
TCGCGAGAAGGATATTTGATCACCGCCCATATCACTGCTGATGCATTTTGCTGGTCGATGGTGCGGTCGCTGATCGGAGC
GTTGCTGGCTGTGGGTGATCACCGACGCACCATCGGCTGGTGTGGTGAGTTGCTCACAAAGGCAAGCCGGTCCAGCGACT
TCACAACTGCGCCGGCACATGGCTTGACGTTTATTCGCGTGGATTATCCACCTGACGATGAGCTGGCTGAGCGGGCCGTG
ATTACCCGGGATCTGCGATCATCGTTCTAG

Upstream 100 bases:

>100_bases
GAACATTAGTGAGGATGTCCGTCTTCGGCTCGACATCGCCTACGACGGAACCGATTTCGCAGGATGGGCGGTCCAAGCGG
GACAGCGCACAGTCGCTGGC

Downstream 100 bases:

>100_bases
GGTGTTGTGGGTGTTTTGTCGGTGGTGGGTGAAATGGCTGTTTTTGCGTTTTATGACTGGCCGATATGTTCGGTAGTCGT
GGGGGGCAGCCCGGAATCCT

Product: putative pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MLDEAFTTVFRTPVRLRAAGRTDTGVHATGQVAHVDIPGTALSKANPRSPHTGDPEFLPLLRRLSRLLPVDVRVLDIACA
PVGFDARFSALRRHYRYRLSTAPYGVQPNQARYVTAWPRELDLDSMSAASRDLLGLRDFAVFCRHRHGATTIRELQRLEW
SREGYLITAHITADAFCWSMVRSLIGALLAVGDHRRTIGWCGELLTKASRSSDFTTAPAHGLTFIRVDYPPDDELAERAV
ITRDLRSSF

Sequences:

>Translated_249_residues
MLDEAFTTVFRTPVRLRAAGRTDTGVHATGQVAHVDIPGTALSKANPRSPHTGDPEFLPLLRRLSRLLPVDVRVLDIACA
PVGFDARFSALRRHYRYRLSTAPYGVQPNQARYVTAWPRELDLDSMSAASRDLLGLRDFAVFCRHRHGATTIRELQRLEW
SREGYLITAHITADAFCWSMVRSLIGALLAVGDHRRTIGWCGELLTKASRSSDFTTAPAHGLTFIRVDYPPDDELAERAV
ITRDLRSSF
>Mature_249_residues
MLDEAFTTVFRTPVRLRAAGRTDTGVHATGQVAHVDIPGTALSKANPRSPHTGDPEFLPLLRRLSRLLPVDVRVLDIACA
PVGFDARFSALRRHYRYRLSTAPYGVQPNQARYVTAWPRELDLDSMSAASRDLLGLRDFAVFCRHRHGATTIRELQRLEW
SREGYLITAHITADAFCWSMVRSLIGALLAVGDHRRTIGWCGELLTKASRSSDFTTAPAHGLTFIRVDYPPDDELAERAV
ITRDLRSSF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=234, Percent_Identity=35.042735042735, Blast_Score=112, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321112, Length=186, Percent_Identity=30.1075268817204, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27868; Mature: 27868

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEAFTTVFRTPVRLRAAGRTDTGVHATGQVAHVDIPGTALSKANPRSPHTGDPEFLPL
CCCHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHH
LRRLSRLLPVDVRVLDIACAPVGFDARFSALRRHYRYRLSTAPYGVQPNQARYVTAWPRE
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCC
LDLDSMSAASRDLLGLRDFAVFCRHRHGATTIRELQRLEWSREGYLITAHITADAFCWSM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
VRSLIGALLAVGDHRRTIGWCGELLTKASRSSDFTTAPAHGLTFIRVDYPPDDELAERAV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
ITRDLRSSF
HHHHHHHCC
>Mature Secondary Structure
MLDEAFTTVFRTPVRLRAAGRTDTGVHATGQVAHVDIPGTALSKANPRSPHTGDPEFLPL
CCCHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHH
LRRLSRLLPVDVRVLDIACAPVGFDARFSALRRHYRYRLSTAPYGVQPNQARYVTAWPRE
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCC
LDLDSMSAASRDLLGLRDFAVFCRHRHGATTIRELQRLEWSREGYLITAHITADAFCWSM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
VRSLIGALLAVGDHRRTIGWCGELLTKASRSSDFTTAPAHGLTFIRVDYPPDDELAERAV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
ITRDLRSSF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]