Click here to switch to the map view.

The map label for this gene is glyA

Identifier: 221230532

GI number: 221230532

Start: 2340079

End: 2341359

Strand: Reverse

Name: glyA

Synonym: MLBr_01953

Alternate gene names: 221230532

Gene position: 2341359-2340079 (Counterclockwise)

Preceding gene: 221230534

Following gene: 221230531

Centisome position: 71.64

GC content: 64.56

Gene sequence:

>1281_bases
ATGGTCGCGCCGCTGGCTGAAGTCGACCCGGATATCGCCGAGCTACTGGGCAAAGAGCTAGGCCGGCAACGGGACACCTT
GGAGATGATCGCTTCAGAGAACTTTGTGCCGCGCTCGGTTCTACAGGCCCAAGGCAGCGTGCTGACCAACAAGTACGCTG
AGGGGTTGCCCGGCCGACGCTATTACGACGGCTGCGAGCACGTCGACGTCGTGGAGAACATCGCCCGCGACCGGGCCAAG
GCGCTGTTCGGTGCCGACTTCGCCAACGTGCAGCCGCACTCGGGGGCCCAGGCCAACGCCGCGGTACTGCACGCGCTGAT
GTCTCCGGGGGAGCGGCTGCTGGGTCTGGATCTCGCCAATGGCGGTCATCTGACGCATGGCATGCGGCTGAACTTCTCCG
GCAAGCTGTATGAAACCGGCTTTTATGGCGTCGACGCGACAACGCATCTCATCGATATGGACGCGGTGCGGGCCAAGGCG
CTCGAATTCCGCCCGAAGGTGCTGATCGCTGGCTGGTCGGCCTATCCGCGGATTCTGGACTTCGCTGCTTTTCGGTCGAT
CGCAGACGAAGTCGGCGCCAAGCTGTGGGTCGACATGGCGCATTTCGCGGGCCTGGTTGCGGTGGGGTTGCACCCGTCTC
CAGTGCCGCATGCAGATGTGGTGTCCACGACCGTTCACAAGACTCTTGGCGGGGGCCGTTCCGGTTTGATCCTGGGCAAG
CAGGAGTTCGCCACGGCCATCAACTCAGCGGTGTTTCCTGGCCAGCAGGGTGGACCGCTTATGCATGTCATCGCGGGCAA
GGCGGTCGCGCTGAAGATTGCTACCACGCCTGAGTTCACCGACCGGCAGCAGCGCACGCTGGCCGGCGCCCGGATTCTCG
CCGATCGGCTTACCGCCGCTGATGTCACCAAGGCCGGGGTGTCGGTGGTCAGTGGTGGCACTGACGTCCACCTAGTGCTG
GTCGACCTGCGCAACTCCCCGTTCGACGGCCAGGCAGCAGAAGATCTGCTGCACGAGGTCGGCATCACTGTCAACCGCAA
CGTGGTTCCCAATGACCCCCGGCCGCCGATGGTGACCTCAGGCCTGCGGATAGGAACCCCCGCGCTGGCAACCCGAGGGT
TCGGTGAAGCGGAGTTCACCGAGGTCGCGGACATCATCGCGACGGTGCTGACCACTGGTGGCAGTGTCGATGTGGCCGCG
CTGCGGCAGCAGGTTACCCGACTTGCCAGGGACTTCCCGCTCTACGGGGGACTTGAGGACTGGAGCTTGGCCGGTCGCTA
G

Upstream 100 bases:

>100_bases
GCACGACGGCTGGGCTGGCTAAAAGCTAACACGCGATAGACTGGCGGCTGTGACTGCCGCATCTGATGCTCGTACCATTC
TTTTCGGCAACGCCTCTCTG

Downstream 100 bases:

>100_bases
GTTACACGTAATCTTCTGATTTAACAAAAACTGTGTAAACGGGTTACAGTTACCTTATGGCACAGAAACCTGTACCTAAC
GCATTGATCCTTCAGCTTGA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MVAPLAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRRYYDGCEHVDVVENIARDRAK
ALFGADFANVQPHSGAQANAAVLHALMSPGERLLGLDLANGGHLTHGMRLNFSGKLYETGFYGVDATTHLIDMDAVRAKA
LEFRPKVLIAGWSAYPRILDFAAFRSIADEVGAKLWVDMAHFAGLVAVGLHPSPVPHADVVSTTVHKTLGGGRSGLILGK
QEFATAINSAVFPGQQGGPLMHVIAGKAVALKIATTPEFTDRQQRTLAGARILADRLTAADVTKAGVSVVSGGTDVHLVL
VDLRNSPFDGQAAEDLLHEVGITVNRNVVPNDPRPPMVTSGLRIGTPALATRGFGEAEFTEVADIIATVLTTGGSVDVAA
LRQQVTRLARDFPLYGGLEDWSLAGR

Sequences:

>Translated_426_residues
MVAPLAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRRYYDGCEHVDVVENIARDRAK
ALFGADFANVQPHSGAQANAAVLHALMSPGERLLGLDLANGGHLTHGMRLNFSGKLYETGFYGVDATTHLIDMDAVRAKA
LEFRPKVLIAGWSAYPRILDFAAFRSIADEVGAKLWVDMAHFAGLVAVGLHPSPVPHADVVSTTVHKTLGGGRSGLILGK
QEFATAINSAVFPGQQGGPLMHVIAGKAVALKIATTPEFTDRQQRTLAGARILADRLTAADVTKAGVSVVSGGTDVHLVL
VDLRNSPFDGQAAEDLLHEVGITVNRNVVPNDPRPPMVTSGLRIGTPALATRGFGEAEFTEVADIIATVLTTGGSVDVAA
LRQQVTRLARDFPLYGGLEDWSLAGR
>Mature_426_residues
MVAPLAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRRYYDGCEHVDVVENIARDRAK
ALFGADFANVQPHSGAQANAAVLHALMSPGERLLGLDLANGGHLTHGMRLNFSGKLYETGFYGVDATTHLIDMDAVRAKA
LEFRPKVLIAGWSAYPRILDFAAFRSIADEVGAKLWVDMAHFAGLVAVGLHPSPVPHADVVSTTVHKTLGGGRSGLILGK
QEFATAINSAVFPGQQGGPLMHVIAGKAVALKIATTPEFTDRQQRTLAGARILADRLTAADVTKAGVSVVSGGTDVHLVL
VDLRNSPFDGQAAEDLLHEVGITVNRNVVPNDPRPPMVTSGLRIGTPALATRGFGEAEFTEVADIIATVLTTGGSVDVAA
LRQQVTRLARDFPLYGGLEDWSLAGR

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=458, Percent_Identity=44.7598253275109, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862352, Length=458, Percent_Identity=44.7598253275109, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI261862350, Length=458, Percent_Identity=44.7598253275109, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI261862348, Length=458, Percent_Identity=44.7598253275109, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI261862346, Length=458, Percent_Identity=44.1048034934498, Blast_Score=331, Evalue=9e-91,
Organism=Homo sapiens, GI22547186, Length=469, Percent_Identity=43.2835820895522, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI22547189, Length=454, Percent_Identity=40.9691629955947, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1788902, Length=415, Percent_Identity=53.4939759036145, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25144732, Length=412, Percent_Identity=44.1747572815534, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25144729, Length=412, Percent_Identity=44.1747572815534, Blast_Score=320, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6319739, Length=407, Percent_Identity=45.4545454545455, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=436, Percent_Identity=40.3669724770642, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24640005, Length=463, Percent_Identity=43.6285097192225, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI221329721, Length=460, Percent_Identity=43.9130434782609, Blast_Score=355, Evalue=2e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_MYCLB (B8ZSH2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503948.1
- ProteinModelPortal:   B8ZSH2
- SMR:   B8ZSH2
- EnsemblBacteria:   EBMYCT00000085116
- GeneID:   7326168
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015531
- HOGENOM:   HBG301263
- OMA:   RPRMLIG
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45225; Mature: 45225

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPLAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRR
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCCCC
YYDGCEHVDVVENIARDRAKALFGADFANVQPHSGAQANAAVLHALMSPGERLLGLDLAN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECC
GGHLTHGMRLNFSGKLYETGFYGVDATTHLIDMDAVRAKALEFRPKVLIAGWSAYPRILD
CCCCCCCEEECCCCEEEECCEECCCCHHHEEEHHHHHHHHHHCCCCEEEECCCHHHHHHH
FAAFRSIADEVGAKLWVDMAHFAGLVAVGLHPSPVPHADVVSTTVHKTLGGGRSGLILGK
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEECH
QEFATAINSAVFPGQQGGPLMHVIAGKAVALKIATTPEFTDRQQRTLAGARILADRLTAA
HHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
DVTKAGVSVVSGGTDVHLVLVDLRNSPFDGQAAEDLLHEVGITVNRNVVPNDPRPPMVTS
HHHHHCHHEECCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEC
GLRIGTPALATRGFGEAEFTEVADIIATVLTTGGSVDVAALRQQVTRLARDFPLYGGLED
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
WSLAGR
CCCCCC
>Mature Secondary Structure
MVAPLAEVDPDIAELLGKELGRQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGLPGRR
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCCCC
YYDGCEHVDVVENIARDRAKALFGADFANVQPHSGAQANAAVLHALMSPGERLLGLDLAN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECC
GGHLTHGMRLNFSGKLYETGFYGVDATTHLIDMDAVRAKALEFRPKVLIAGWSAYPRILD
CCCCCCCEEECCCCEEEECCEECCCCHHHEEEHHHHHHHHHHCCCCEEEECCCHHHHHHH
FAAFRSIADEVGAKLWVDMAHFAGLVAVGLHPSPVPHADVVSTTVHKTLGGGRSGLILGK
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEECH
QEFATAINSAVFPGQQGGPLMHVIAGKAVALKIATTPEFTDRQQRTLAGARILADRLTAA
HHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
DVTKAGVSVVSGGTDVHLVLVDLRNSPFDGQAAEDLLHEVGITVNRNVVPNDPRPPMVTS
HHHHHCHHEECCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEC
GLRIGTPALATRGFGEAEFTEVADIIATVLTTGGSVDVAALRQQVTRLARDFPLYGGLED
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
WSLAGR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA