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The map label for this gene is fumC [H]

Identifier: 221230527

GI number: 221230527

Start: 2333549

End: 2334973

Strand: Direct

Name: fumC [H]

Synonym: MLBr_01947

Alternate gene names: 221230527

Gene position: 2333549-2334973 (Clockwise)

Preceding gene: 221230526

Following gene: 221230533

Centisome position: 71.4

GC content: 60.49

Gene sequence:

>1425_bases
ATGAAGAGGACCCAATTCATGGCCGACAGCGCCGAATACCGCATCGAGCACGACACCATGGGCGAAGTCCGGGTACCGGC
AATAGCATTGTGGCGCGCGCAAACCCAGCGCGCGGTGGAGAACTTCCCAATCTCCGGTCGAGGTTTAGAGCGCACCCAAA
TCCGAGCGTTGGGTCTGCTGAAAGGCGCTTGCGCGCAGGTCAACAAAGACCTGGGATTGTTGGCTTCGGAGAAAGCCAAC
GCAATCATTGCCGCGGCGGCCACAATCGCCGACGGCTACCACGACGACCAATTCCCGATTGACGTATTCCAAACCGGATC
CGGCACCAGCTCCAACATGAACACCAACGAGGTTATCGGCAGCATCGCGGCAGCCAACGGTGTACCGGTGCATCCCAACG
ACGACGTCAACATGTCGCAATCATCCAACGACACATTCCCGACCGCTACCCACATCGCAGCCACCGAAGCCGCGGTACGC
CATCTGATTCCGGCGCTGCAAGAGCTGCACGCCGCACTGGCGACCAAGTCCAGCGAGTGGCACACGGTGGTGAAATCGGG
TCGCACGCACTTGATGGACGCCGTTCCGGTAACACTCGGCCAGGAGTTCGGTGGATACGCTCGCCAAATCGAGGCCGGAA
TCGAGCGCGTACGCGCCACGCTGCCACGGCTTGGTGAACTGGCAATCGGCGGCACCGCAGTAGGCACCGGCCTCAACGCT
CCCGATGGCTTCGGCGCTAAGGTGGTCGAGGCACTGATCGCTACCACGGGTCTGACCGAACTACGACCGGCAGCAAACTC
ATTCGAGGCACAGGCCGCCCGAGACGGGCTTGTAGAGGCATCCGGGGCGCTGCGCACTATCGCGGTGTCACTGACTAAGA
TTGCCAACGATATTCGGTTGATGGGATCCGGTCCGCTGACCGGCCTGGCGGAGATCCAGCTGCCAGACTTACAGCCGGGT
AGCTCGATCATGCCAGGCAAGGTAAATCCGGTTCTTCCGGAGGCGGTTACCCAGGTTGCCGCGCAGGTGATTGGCAATGA
TGCCGCCGTTGCCTGGGGCGGTGCAAATGGAGCCTTTGAGCTCAACGTCTATATCCCGATGATGGCCCGCAACATCTTGG
AGTCGTACCAGCTGCTAACCAACGTGTCCAGGCTGTTTGCGGAGCGTTGTATTAAAGGGCTTTCCGCCAATATCGAGCAC
CTGCGCGAGCTGGCCGAGTCGTCGCAGTCGATCGTGACACCCTTGAATTCGGCCATCGGATACGAGGAGGCTGCCGCGGT
GGTCAAACAAGCACTCAAGGAACGCAAGACCCTCCGTCAGACCGTTATCGACCGCGGCTTGATCGGGGACAAGCTGTCCC
TCGAGGAGCTGGACCGCCGACTTGATGTGCTGGCGATGGCAAAAGTCAAGCCAGCGGATTCCTAA

Upstream 100 bases:

>100_bases
TGATCGAGGCCTACCACCGGCTCTCTAAACTCAACGAATACTCCGCCATCGACTTCACCGGCGATAACAACGCCGCCTAC
CCCCTGCCGTAAACCACGCA

Downstream 100 bases:

>100_bases
CCGAATTGAGGAAAATTAGTCGAGGCCAGATGGCGCCAAGACCTACCTGCGTGGCCTCCGAGCGCCCGCACAGCACCATG
GAACAGCGTTCGACCCAGTA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKRTQFMADSAEYRIEHDTMGEVRVPAIALWRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNKDLGLLASEKAN
AIIAAAATIADGYHDDQFPIDVFQTGSGTSSNMNTNEVIGSIAAANGVPVHPNDDVNMSQSSNDTFPTATHIAATEAAVR
HLIPALQELHAALATKSSEWHTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVRATLPRLGELAIGGTAVGTGLNA
PDGFGAKVVEALIATTGLTELRPAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRLMGSGPLTGLAEIQLPDLQPG
SSIMPGKVNPVLPEAVTQVAAQVIGNDAAVAWGGANGAFELNVYIPMMARNILESYQLLTNVSRLFAERCIKGLSANIEH
LRELAESSQSIVTPLNSAIGYEEAAAVVKQALKERKTLRQTVIDRGLIGDKLSLEELDRRLDVLAMAKVKPADS

Sequences:

>Translated_474_residues
MKRTQFMADSAEYRIEHDTMGEVRVPAIALWRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNKDLGLLASEKAN
AIIAAAATIADGYHDDQFPIDVFQTGSGTSSNMNTNEVIGSIAAANGVPVHPNDDVNMSQSSNDTFPTATHIAATEAAVR
HLIPALQELHAALATKSSEWHTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVRATLPRLGELAIGGTAVGTGLNA
PDGFGAKVVEALIATTGLTELRPAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRLMGSGPLTGLAEIQLPDLQPG
SSIMPGKVNPVLPEAVTQVAAQVIGNDAAVAWGGANGAFELNVYIPMMARNILESYQLLTNVSRLFAERCIKGLSANIEH
LRELAESSQSIVTPLNSAIGYEEAAAVVKQALKERKTLRQTVIDRGLIGDKLSLEELDRRLDVLAMAKVKPADS
>Mature_474_residues
MKRTQFMADSAEYRIEHDTMGEVRVPAIALWRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNKDLGLLASEKAN
AIIAAAATIADGYHDDQFPIDVFQTGSGTSSNMNTNEVIGSIAAANGVPVHPNDDVNMSQSSNDTFPTATHIAATEAAVR
HLIPALQELHAALATKSSEWHTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVRATLPRLGELAIGGTAVGTGLNA
PDGFGAKVVEALIATTGLTELRPAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRLMGSGPLTGLAEIQLPDLQPG
SSIMPGKVNPVLPEAVTQVAAQVIGNDAAVAWGGANGAFELNVYIPMMARNILESYQLLTNVSRLFAERCIKGLSANIEH
LRELAESSQSIVTPLNSAIGYEEAAAVVKQALKERKTLRQTVIDRGLIGDKLSLEELDRRLDVLAMAKVKPADS

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=476, Percent_Identity=51.0504201680672, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1787896, Length=428, Percent_Identity=49.7663551401869, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI87082375, Length=464, Percent_Identity=40.5172413793103, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17553882, Length=451, Percent_Identity=50.3325942350333, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565146, Length=314, Percent_Identity=51.9108280254777, Blast_Score=317, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6324993, Length=454, Percent_Identity=49.5594713656388, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI78710009, Length=451, Percent_Identity=49.8891352549889, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24640179, Length=452, Percent_Identity=50.2212389380531, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24640177, Length=452, Percent_Identity=50.2212389380531, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24662684, Length=447, Percent_Identity=48.5458612975391, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24583245, Length=445, Percent_Identity=41.5730337078652, Blast_Score=341, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 50185; Mature: 50185

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTQFMADSAEYRIEHDTMGEVRVPAIALWRAQTQRAVENFPISGRGLERTQIRALGLL
CCCCHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KGACAQVNKDLGLLASEKANAIIAAAATIADGYHDDQFPIDVFQTGSGTSSNMNTNEVIG
HHHHHHHCCCCCCEECCCCCEEEEHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
SIAAANGVPVHPNDDVNMSQSSNDTFPTATHIAATEAAVRHLIPALQELHAALATKSSEW
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVRATLPRLGELAIGGTAVGTGLNA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCC
PDGFGAKVVEALIATTGLTELRPAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRL
CCCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHEE
MGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEAVTQVAAQVIGNDAAVAWGGANGAFE
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEE
LNVYIPMMARNILESYQLLTNVSRLFAERCIKGLSANIEHLRELAESSQSIVTPLNSAIG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
YEEAAAVVKQALKERKTLRQTVIDRGLIGDKLSLEELDRRLDVLAMAKVKPADS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRTQFMADSAEYRIEHDTMGEVRVPAIALWRAQTQRAVENFPISGRGLERTQIRALGLL
CCCCHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KGACAQVNKDLGLLASEKANAIIAAAATIADGYHDDQFPIDVFQTGSGTSSNMNTNEVIG
HHHHHHHCCCCCCEECCCCCEEEEHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
SIAAANGVPVHPNDDVNMSQSSNDTFPTATHIAATEAAVRHLIPALQELHAALATKSSEW
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HTVVKSGRTHLMDAVPVTLGQEFGGYARQIEAGIERVRATLPRLGELAIGGTAVGTGLNA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCC
PDGFGAKVVEALIATTGLTELRPAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRL
CCCCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHEE
MGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEAVTQVAAQVIGNDAAVAWGGANGAFE
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEE
LNVYIPMMARNILESYQLLTNVSRLFAERCIKGLSANIEHLRELAESSQSIVTPLNSAIG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
YEEAAAVVKQALKERKTLRQTVIDRGLIGDKLSLEELDRRLDVLAMAKVKPADS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]