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The map label for this gene is galE [C]

Identifier: 221230524

GI number: 221230524

Start: 2327868

End: 2328998

Strand: Direct

Name: galE [C]

Synonym: MLBr_01942

Alternate gene names: 221230524

Gene position: 2327868-2328998 (Clockwise)

Preceding gene: 221230523

Following gene: 221230526

Centisome position: 71.23

GC content: 57.65

Gene sequence:

>1131_bases
ATGCTTCGCGGCGTGGGTAACGCATCGTTGACGACCGAGCTCGGACGCGTCTTGGTCACCGGCGGTTCGGGCTTCGTCGG
CGCTAATCTGGTGACCGCCTTGCTGGAACGCGGATACCAGGTACGCTCTTTCGACCGCGCCCCGATGCCGCTGCCGCAGC
ACCCGCAGCTGGAGGTGCTGCAAGGTGACATCACCGACGCTACGGTCTGCACTACAGCGATGGACAGCATCGATACAGTC
TTTCACACCGCGGCAATCATCGAGCTAATGGGCGGCGCTTCGGTGACCGACGAGTATCGGCAGCGCAGCTACACGGTCAA
CGTGGGCGGTACCGAAAACTTGCTCCGCGCAGGACAAAAGTCCGGAGTCAAACGGTTCGTCTACACCGCATCCAACAGTG
TGGTGATGGGGGGCACTCCCATCACCGGGGGTGATGAAACAATGCCCTATACCAAGCGGTTCAACGACCTGTACACCGAA
ACCAAAGTGGTCGCCGAAAAATTCGTACTCTCACAAAACGGCGTACCCGATGGTGAAACCATGCTAACATGTTCGATCAG
ACCCAGTGGCATATGGGGTCGCGGCGACCAGACAATGTTCCGTAAGGCATTCGAAAGTGTCGTATCCGGTCACGTCAAGG
TACTCATCGGGAGTAAGAACGCCAAACTGGATAACTCCTACGTGCACAACCTGGTGCACGGCTTAATCTTGGCTGCCGAG
CACCTGGTCCCAGGCGGCACCGCACCTGGACAAGCCTACTTCATCAACGATGGCGAGCCGATCAACTTCTTCGATTTCAT
GGGGCCAATCATCAAGGCGTGTGGTGAAAATTGGCCACGGGTGCGGATCTCCGGTCGGCTGGTGCGCAACGTGATGGCTG
TGTGGCAGCGGCTACACTTCGGCTTCGGGCTTCCGAAGCCTCCGATGGAACCGCTTGCGGTAGAACGAGTTTACCTCGAC
AACTACTTTTCCATCGAAAAGGCGCACAAAGAGCTGGGCTATCGGCCGCTGTTCACTACCGAACAGGCCATGGCTGAATG
TCTACCTTACTACACAGAGTTATTCGAACAAATCAAGGTGGCTGCCAAGCCCCATTTGGCATCCATAGCCGCCACCCCGC
GGCCCGAGTAG

Upstream 100 bases:

>100_bases
GCCAGCGAGTCGCCGATGTGCTCTCCGCCAGGCAGGTCGAAGAAGCCTAATGCCGATCAAATTAAAGACCGGTTTCCTTT
AACTTAAACACGTGTCAGTT

Downstream 100 bases:

>100_bases
CGGGCGCACAAGCATTGAAACTGCAAGCTGTGCCAATATTTTCGGGTGGGTGCAACCGTTTAAGTGTAGCGGCAGCTCCG
ACGCATGAATGTGTTGCGCT

Product: putative cholesterol dehydrogenase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMPLPQHPQLEVLQGDITDATVCTTAMDSIDTV
FHTAAIIELMGGASVTDEYRQRSYTVNVGGTENLLRAGQKSGVKRFVYTASNSVVMGGTPITGGDETMPYTKRFNDLYTE
TKVVAEKFVLSQNGVPDGETMLTCSIRPSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAE
HLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVMAVWQRLHFGFGLPKPPMEPLAVERVYLD
NYFSIEKAHKELGYRPLFTTEQAMAECLPYYTELFEQIKVAAKPHLASIAATPRPE

Sequences:

>Translated_376_residues
MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMPLPQHPQLEVLQGDITDATVCTTAMDSIDTV
FHTAAIIELMGGASVTDEYRQRSYTVNVGGTENLLRAGQKSGVKRFVYTASNSVVMGGTPITGGDETMPYTKRFNDLYTE
TKVVAEKFVLSQNGVPDGETMLTCSIRPSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAE
HLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVMAVWQRLHFGFGLPKPPMEPLAVERVYLD
NYFSIEKAHKELGYRPLFTTEQAMAECLPYYTELFEQIKVAAKPHLASIAATPRPE
>Mature_376_residues
MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMPLPQHPQLEVLQGDITDATVCTTAMDSIDTV
FHTAAIIELMGGASVTDEYRQRSYTVNVGGTENLLRAGQKSGVKRFVYTASNSVVMGGTPITGGDETMPYTKRFNDLYTE
TKVVAEKFVLSQNGVPDGETMLTCSIRPSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAE
HLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVMAVWQRLHFGFGLPKPPMEPLAVERVYLD
NYFSIEKAHKELGYRPLFTTEQAMAECLPYYTELFEQIKVAAKPHLASIAATPRPE

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI239745448, Length=337, Percent_Identity=34.1246290801187, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI310132178, Length=337, Percent_Identity=34.1246290801187, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI310113012, Length=337, Percent_Identity=34.1246290801187, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI116268111, Length=333, Percent_Identity=35.7357357357357, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI193211614, Length=350, Percent_Identity=32.5714285714286, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI8393516, Length=350, Percent_Identity=32.5714285714286, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI260763931, Length=374, Percent_Identity=29.6791443850267, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4504509, Length=374, Percent_Identity=29.6791443850267, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI19923621, Length=353, Percent_Identity=30.8781869688385, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4504507, Length=376, Percent_Identity=28.9893617021277, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI218563686, Length=191, Percent_Identity=34.0314136125654, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI218563684, Length=191, Percent_Identity=34.0314136125654, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786974, Length=294, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17570557, Length=361, Percent_Identity=28.808864265928, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI193211272, Length=347, Percent_Identity=29.3948126801153, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17509709, Length=375, Percent_Identity=28.5333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321437, Length=351, Percent_Identity=28.2051282051282, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 41164; Mature: 41164

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMPLPQHPQLEVL
CCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEE
QGDITDATVCTTAMDSIDTVFHTAAIIELMGGASVTDEYRQRSYTVNVGGTENLLRAGQK
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCEEEEECCCHHHHHHHHHH
SGVKRFVYTASNSVVMGGTPITGGDETMPYTKRFNDLYTETKVVAEKFVLSQNGVPDGET
CCCEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
MLTCSIRPSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAE
EEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
HLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVMAVWQRLHF
HHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
GFGLPKPPMEPLAVERVYLDNYFSIEKAHKELGYRPLFTTEQAMAECLPYYTELFEQIKV
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAKPHLASIAATPRPE
HCCCCHHHHCCCCCCC
>Mature Secondary Structure
MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMPLPQHPQLEVL
CCCCCCCCHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEE
QGDITDATVCTTAMDSIDTVFHTAAIIELMGGASVTDEYRQRSYTVNVGGTENLLRAGQK
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCEEEEECCCHHHHHHHHHH
SGVKRFVYTASNSVVMGGTPITGGDETMPYTKRFNDLYTETKVVAEKFVLSQNGVPDGET
CCCEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
MLTCSIRPSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAE
EEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
HLVPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVMAVWQRLHF
HHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
GFGLPKPPMEPLAVERVYLDNYFSIEKAHKELGYRPLFTTEQAMAECLPYYTELFEQIKV
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAKPHLASIAATPRPE
HCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]