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The map label for this gene is eftA

Identifier: 221230392

GI number: 221230392

Start: 2064002

End: 2064958

Strand: Reverse

Name: eftA

Synonym: MLBr_01711

Alternate gene names: 221230392

Gene position: 2064958-2064002 (Counterclockwise)

Preceding gene: 221230393

Following gene: 221230391

Centisome position: 63.19

GC content: 64.99

Gene sequence:

>957_bases
ATGGCTGAAGCACTGGTGCTTGTCGAACACACTGAAGGCGCGCTGAAAAAGGTCAGCGCTGAATTGATCACCGCCGCCCG
CGTGCTGGGCGAGCCGGCTGCCGTCGTGGTCGGTACACCGGGAACGTCGGCGCCGCTGGTTGACGGGTTGAAGACGGCTG
GCGCTGCCAAAATCTACGTCGCCGAGTCCGACGCCGCCGACAAATACTTGATCACGCCGGTAGTCGATGTGCTGGCCGCG
TTGGCCGAGTCATCGGCTCCGGCCGCTGTGTTGCTGGCCGCCACCGCCGATGGCAAGGAGATCGGCGGCCGGCTGGCGGC
CCGGATCGGGTCCGGTCTGTTGGTCGATGTCGTGGACGTTCGAGAAGGAGCGGTGGGTGTCCATAGCGTTTTCGGTGGTG
TGTTTATTGTAGAGGCGCAGGCCAACGGTGACACTCCGGTGATCACAGTACGGGCTGGGGCCGTTGAGGCGCAGCCAGCC
GAGGGGGCGGGCGAGCAGGTCAGCGTGGAAGTGCCGGCTCCTGCCGAGAACGCCACCAAGATCACCGCCCGTGCGCCCGC
GGTTGTCGACAACCGCCCAGACTTGACCGAGGCCACCGTCGTGGTGTCCGGCGGCCGTGGTGTTGGCAGTGCGGATAACT
TCAGCGTGGTCGAGGCGCTGGCCGACTCGCTGGGCGCCGCGGTTGGAGCATCGCGTGCCGCTGTGGATTCCGGCTACTAC
CCGGGCCAGTTCCAAATAGGCCAGACTGGTAAGACGGTGTCGCCCCAGCTCTACATCGCGCTGGGTATCTCCGGGGCTAT
CCAGCACCGCGCGGGCATGCAGACCTCGAAGACCATCGTTGCGGTCAACAAGGACGAGGAAGCACCGATCTTCGAGATCG
CGGACTTCGGTGTCGTCGGTGACTTGTTTAAGGTGGCGCCACAGCTGACCGACGGGATCAAGGCTCGTAAGGGCTAG

Upstream 100 bases:

>100_bases
CGATGGCGGTAACCAGATCGTGCAGTACCTGGTTGCTCAGAAGATCATCTAAGCCGAACGCTACAAACCAATCCCCGAGA
CGAGAAGAGCGATATCATCC

Downstream 100 bases:

>100_bases
GTTTTCGCGCGGGCCCGTCGTGGTGTGCATCCGCCACATGTGCGCCAGGGGAACCATCGCACGATCCGTGAATACATTAT
CGCATGTGATACTGCATTCG

Product: electron transfer flavoprotein alpha subunit

Products: NA

Alternate protein names: Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAEALVLVEHTEGALKKVSAELITAARVLGEPAAVVVGTPGTSAPLVDGLKTAGAAKIYVAESDAADKYLITPVVDVLAA
LAESSAPAAVLLAATADGKEIGGRLAARIGSGLLVDVVDVREGAVGVHSVFGGVFIVEAQANGDTPVITVRAGAVEAQPA
EGAGEQVSVEVPAPAENATKITARAPAVVDNRPDLTEATVVVSGGRGVGSADNFSVVEALADSLGAAVGASRAAVDSGYY
PGQFQIGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADFGVVGDLFKVAPQLTDGIKARKG

Sequences:

>Translated_318_residues
MAEALVLVEHTEGALKKVSAELITAARVLGEPAAVVVGTPGTSAPLVDGLKTAGAAKIYVAESDAADKYLITPVVDVLAA
LAESSAPAAVLLAATADGKEIGGRLAARIGSGLLVDVVDVREGAVGVHSVFGGVFIVEAQANGDTPVITVRAGAVEAQPA
EGAGEQVSVEVPAPAENATKITARAPAVVDNRPDLTEATVVVSGGRGVGSADNFSVVEALADSLGAAVGASRAAVDSGYY
PGQFQIGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADFGVVGDLFKVAPQLTDGIKARKG
>Mature_317_residues
AEALVLVEHTEGALKKVSAELITAARVLGEPAAVVVGTPGTSAPLVDGLKTAGAAKIYVAESDAADKYLITPVVDVLAAL
AESSAPAAVLLAATADGKEIGGRLAARIGSGLLVDVVDVREGAVGVHSVFGGVFIVEAQANGDTPVITVRAGAVEAQPAE
GAGEQVSVEVPAPAENATKITARAPAVVDNRPDLTEATVVVSGGRGVGSADNFSVVEALADSLGAAVGASRAAVDSGYYP
GQFQIGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADFGVVGDLFKVAPQLTDGIKARKG

Specific function: The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family

Homologues:

Organism=Homo sapiens, GI4503607, Length=318, Percent_Identity=40.251572327044, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI189181759, Length=274, Percent_Identity=41.2408759124088, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1786226, Length=251, Percent_Identity=37.4501992031873, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787990, Length=296, Percent_Identity=33.7837837837838, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI87082157, Length=245, Percent_Identity=31.4285714285714, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17506929, Length=312, Percent_Identity=38.7820512820513, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6325261, Length=321, Percent_Identity=33.0218068535826, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17136898, Length=323, Percent_Identity=42.4148606811146, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24652801, Length=323, Percent_Identity=42.4148606811146, Blast_Score=208, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ETFA_MYCLE (O33096)

Other databases:

- EMBL:   Z99263
- EMBL:   AL583923
- PIR:   T45397
- RefSeq:   NP_302178.1
- ProteinModelPortal:   O33096
- SMR:   O33096
- EnsemblBacteria:   EBMYCT00000029390
- GeneID:   910783
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1711
- NMPDR:   fig|272631.1.peg.1050
- Leproma:   ML1711
- GeneTree:   EBGT00050000017730
- HOGENOM:   HBG615986
- OMA:   EANIFTV
- ProtClustDB:   CLSK792228
- BioCyc:   MLEP272631:ML1711-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF000089
- SMART:   SM00893

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha

EC number: NA

Molecular weight: Translated: 31837; Mature: 31706

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEALVLVEHTEGALKKVSAELITAARVLGEPAAVVVGTPGTSAPLVDGLKTAGAAKIYV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCEEEEE
AESDAADKYLITPVVDVLAALAESSAPAAVLLAATADGKEIGGRLAARIGSGLLVDVVDV
EECCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEC
REGAVGVHSVFGGVFIVEAQANGDTPVITVRAGAVEAQPAEGAGEQVSVEVPAPAENATK
CCCCCHHHHHHCCEEEEEEECCCCCCEEEEEECCEECCCCCCCCCEEEEECCCCCCCCEE
ITARAPAVVDNRPDLTEATVVVSGGRGVGSADNFSVVEALADSLGAAVGASRAAVDSGYY
EEECCCEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
PGQFQIGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADFGVVG
CCEEEECCCCCCCCCEEEEEEECCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHH
DLFKVAPQLTDGIKARKG
HHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
AEALVLVEHTEGALKKVSAELITAARVLGEPAAVVVGTPGTSAPLVDGLKTAGAAKIYV
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCEEEEE
AESDAADKYLITPVVDVLAALAESSAPAAVLLAATADGKEIGGRLAARIGSGLLVDVVDV
EECCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEC
REGAVGVHSVFGGVFIVEAQANGDTPVITVRAGAVEAQPAEGAGEQVSVEVPAPAENATK
CCCCCHHHHHHCCEEEEEEECCCCCCEEEEEECCEECCCCCCCCCEEEEECCCCCCCCEE
ITARAPAVVDNRPDLTEATVVVSGGRGVGSADNFSVVEALADSLGAAVGASRAAVDSGYY
EEECCCEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
PGQFQIGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADFGVVG
CCEEEECCCCCCCCCEEEEEEECCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHH
DLFKVAPQLTDGIKARKG
HHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002