Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 221230391

GI number: 221230391

Start: 2060816

End: 2062048

Strand: Reverse

Name: iscS [H]

Synonym: MLBr_01708

Alternate gene names: 221230391

Gene position: 2062048-2060816 (Counterclockwise)

Preceding gene: 221230392

Following gene: 221230390

Centisome position: 63.1

GC content: 63.58

Gene sequence:

>1233_bases
TTGATCCGGCAGGCTCCGATCGCCGGTATCCTAAAGTGCGTCATGGCCTACCTGGATCACGCCGCTACTACCCCGATGCA
CCCCGCCGCCATCGAGGCGATGACGACCGCGCTAGGCACTGTCGGCAACGCGTCGTCGCTGCACACCAGTGGGCGAGTGG
CGCGGCGGCGGATTGAGGAATCCCGCGAGCTGATCGCCGACAAACTTGCTGCGCGCCCGTCCGAGGTGATTTTCACTGCA
GGTGGCACCGAGAGCGACAACTTGGCCGTCAAAGGCATCTATTGGGCGCGCCGTGACGCGCAGCCGTGCCACCGACGCAT
CGTTACAACCGAGGTGGAACACCACGCCGTGTTGGACTCGGTGAACTGGCTGGTCGAACACGAAGGTGCGGAGGTGACGT
GGTTGCCCACCGCTGCCGACGGCTCGGTATCGGCGTTCGGGCTGCGGGAGGCGCTGCAGCAACACAACGACGTCGCACTC
GTATCGGTGATGTGGGCCAATAACGAAGTCGGCACAGTCATGCCAGTCGTCGAACTCGCCGCCGTCGCTGCCGAGTTCGG
TGTTCCGATGCACAGTGATGCGATACAAGCGGTGGGTCAGCTGCCGGTAAAGTTCGGTGTCAGCGGGCTGTCGGCGATGA
GTGTGGCCGCGCATAAATTTGGTGGCCCGCCTGGTGTGGGCGCGTTGCTGCTGCGCCGGGACATCAGCTGTGTTCCGTTG
TTGCATGGTGGTGGGCAGGAGCGCGATATTCGTTCCGGCACTCCCGGTGTCGCCAGCGTTGTTGGGATGGCAGCGGCGGC
ACAGATCGCGGTGGACGGATTGGAGATCAACAGTATCCGGCTGCAGGTGTTGCGTGACCGCTTGATCGAGGGTGTGCTGG
CGACGATTGACGACGTTGCACTCAACGGTGCCCGCGATCGGATGCGGCTTCCAGGTAACGCGCACTTCATCTTCCGTGGT
TGCGAAGGTGATGTGCTATTGATGCTGTTGGATGCCAACGGAATCGAATGCTCGACTGGATCGGCCTGCACCTCCGGTGT
TCCGCAGCCGTCGCACGTTCTGATAGCGATGGGCGCCGACCCGGCTAGTGCGCGCGGATCGCTTCGTCTTTCTTTGGGGC
ACAATAGTGTTGAGGTCGACGTTGAAGAGGTGCTGCGGGTGCTGCCGGGGGCGGTGGATCGCGCCCGCCGAGCATCGCTG
GCAGCGGCAGGGGCCGGGGGTGTTGGGGTGTGA

Upstream 100 bases:

>100_bases
CCCTGGCCCGTCGCTGTCAGTTCACGGTGCGACTGGGGGCTAGGCAGTATGTCGATGCGGAACCTCGACATGTCGGTGCT
GGAGTGAGCAGCAGGCGCTC

Downstream 100 bases:

>100_bases
AAGTTCTCGCCGCGATGAGCGGTGGCGTCGACTCGTCGGTTGCTGCTGCCCGGATGGTCGACGCCGGACATGATGTGGTG
GGTGTTCACCTGGCGCTGTC

Product: putative aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MIRQAPIAGILKCVMAYLDHAATTPMHPAAIEAMTTALGTVGNASSLHTSGRVARRRIEESRELIADKLAARPSEVIFTA
GGTESDNLAVKGIYWARRDAQPCHRRIVTTEVEHHAVLDSVNWLVEHEGAEVTWLPTAADGSVSAFGLREALQQHNDVAL
VSVMWANNEVGTVMPVVELAAVAAEFGVPMHSDAIQAVGQLPVKFGVSGLSAMSVAAHKFGGPPGVGALLLRRDISCVPL
LHGGGQERDIRSGTPGVASVVGMAAAAQIAVDGLEINSIRLQVLRDRLIEGVLATIDDVALNGARDRMRLPGNAHFIFRG
CEGDVLLMLLDANGIECSTGSACTSGVPQPSHVLIAMGADPASARGSLRLSLGHNSVEVDVEEVLRVLPGAVDRARRASL
AAAGAGGVGV

Sequences:

>Translated_410_residues
MIRQAPIAGILKCVMAYLDHAATTPMHPAAIEAMTTALGTVGNASSLHTSGRVARRRIEESRELIADKLAARPSEVIFTA
GGTESDNLAVKGIYWARRDAQPCHRRIVTTEVEHHAVLDSVNWLVEHEGAEVTWLPTAADGSVSAFGLREALQQHNDVAL
VSVMWANNEVGTVMPVVELAAVAAEFGVPMHSDAIQAVGQLPVKFGVSGLSAMSVAAHKFGGPPGVGALLLRRDISCVPL
LHGGGQERDIRSGTPGVASVVGMAAAAQIAVDGLEINSIRLQVLRDRLIEGVLATIDDVALNGARDRMRLPGNAHFIFRG
CEGDVLLMLLDANGIECSTGSACTSGVPQPSHVLIAMGADPASARGSLRLSLGHNSVEVDVEEVLRVLPGAVDRARRASL
AAAGAGGVGV
>Mature_410_residues
MIRQAPIAGILKCVMAYLDHAATTPMHPAAIEAMTTALGTVGNASSLHTSGRVARRRIEESRELIADKLAARPSEVIFTA
GGTESDNLAVKGIYWARRDAQPCHRRIVTTEVEHHAVLDSVNWLVEHEGAEVTWLPTAADGSVSAFGLREALQQHNDVAL
VSVMWANNEVGTVMPVVELAAVAAEFGVPMHSDAIQAVGQLPVKFGVSGLSAMSVAAHKFGGPPGVGALLLRRDISCVPL
LHGGGQERDIRSGTPGVASVVGMAAAAQIAVDGLEINSIRLQVLRDRLIEGVLATIDDVALNGARDRMRLPGNAHFIFRG
CEGDVLLMLLDANGIECSTGSACTSGVPQPSHVLIAMGADPASARGSLRLSLGHNSVEVDVEEVLRVLPGAVDRARRASL
AAAGAGGVGV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=390, Percent_Identity=37.6923076923077, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=33.0917874396135, Blast_Score=178, Evalue=7e-45,
Organism=Escherichia coli, GI48994898, Length=389, Percent_Identity=38.3033419023136, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=25.2525252525253, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789175, Length=406, Percent_Identity=27.3399014778325, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=36.1757105943152, Blast_Score=227, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17533177, Length=326, Percent_Identity=32.5153374233129, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=36.1757105943152, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI20129463, Length=390, Percent_Identity=36.9230769230769, Blast_Score=222, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42757; Mature: 42757

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQAPIAGILKCVMAYLDHAATTPMHPAAIEAMTTALGTVGNASSLHTSGRVARRRIEE
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SRELIADKLAARPSEVIFTAGGTESDNLAVKGIYWARRDAQPCHRRIVTTEVEHHAVLDS
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHH
VNWLVEHEGAEVTWLPTAADGSVSAFGLREALQQHNDVALVSVMWANNEVGTVMPVVELA
HHHHEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
AVAAEFGVPMHSDAIQAVGQLPVKFGVSGLSAMSVAAHKFGGPPGVGALLLRRDISCVPL
HHHHHHCCCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEE
LHGGGQERDIRSGTPGVASVVGMAAAAQIAVDGLEINSIRLQVLRDRLIEGVLATIDDVA
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCEEEHHHHHHHHHHHHHHHHHHHHHHHH
LNGARDRMRLPGNAHFIFRGCEGDVLLMLLDANGIECSTGSACTSGVPQPSHVLIAMGAD
CCCCHHHEECCCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHCCCCCCCCEEEEEECCC
PASARGSLRLSLGHNSVEVDVEEVLRVLPGAVDRARRASLAAAGAGGVGV
CCCCCCCEEEEECCCCEEECHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIRQAPIAGILKCVMAYLDHAATTPMHPAAIEAMTTALGTVGNASSLHTSGRVARRRIEE
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SRELIADKLAARPSEVIFTAGGTESDNLAVKGIYWARRDAQPCHRRIVTTEVEHHAVLDS
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHH
VNWLVEHEGAEVTWLPTAADGSVSAFGLREALQQHNDVALVSVMWANNEVGTVMPVVELA
HHHHEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
AVAAEFGVPMHSDAIQAVGQLPVKFGVSGLSAMSVAAHKFGGPPGVGALLLRRDISCVPL
HHHHHHCCCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEE
LHGGGQERDIRSGTPGVASVVGMAAAAQIAVDGLEINSIRLQVLRDRLIEGVLATIDDVA
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCEEEHHHHHHHHHHHHHHHHHHHHHHHH
LNGARDRMRLPGNAHFIFRGCEGDVLLMLLDANGIECSTGSACTSGVPQPSHVLIAMGAD
CCCCHHHEECCCCCEEEEECCCCCEEEEEEECCCCCCCCCCHHCCCCCCCCEEEEEECCC
PASARGSLRLSLGHNSVEVDVEEVLRVLPGAVDRARRASLAAAGAGGVGV
CCCCCCCEEEEECCCCEEECHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]