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The map label for this gene is gatA

Identifier: 221230385

GI number: 221230385

Start: 2053044

End: 2054537

Strand: Reverse

Name: gatA

Synonym: MLBr_01702

Alternate gene names: 221230385

Gene position: 2054537-2053044 (Counterclockwise)

Preceding gene: 221230386

Following gene: 221230384

Centisome position: 62.87

GC content: 64.26

Gene sequence:

>1494_bases
ATGATTGGTTCACTGCACGAGATCATTCGGTTGGACGCGTCGACGCTGGCTGCCAAGATCGTTGCCAAGGAGCTGTCGTC
GGTTGAGATCACCCAGGCCTGCCTGGACCAGATCGAGGCGACGGACGACACCTACCGAGCCTTCCTTCACGTGGCGGCCG
AGAAGGCACTGTCGGCGGCGGCCGCCGTCGACAAGGCGGTGGCCGCTGGCGGACAGTTGTCGTCAACGTTGGCCGGAGTT
CCATTGGCGCTCAAAGATGTGTTCACCACTGTCGACATGCCGACCACCTGCGGATCCAAGATCCTACAGGGCTGGCATTC
CCCTTATGACGCCACTGTCACCACGCGGTTGCGCGCGGCCGGCATCCCGATCCTAGGTAAGACCAACATGGATGAATTCG
CGATGGGCAGCTCAACCGAGAATTCCGCTTACGGCCCGACCCGCAACCCGTGGAATGTCGACCGGGTGCCAGGCGGCTCC
GGTGGCGGCAGCGCAGCGGCGTTGGCAGCGTTCCAGGCGCCGTTGGCCATCGGCTCCGACACCGGCGGCTCCATCCGGCA
GCCGGCCGCGTTGACCGCGACAGTCGGTGTCAAACCCACCTATGGCACAGTTTCCCGGTACGGACTGGTTGCTTGTGCCT
CGTCGCTGGATCAGGGAGGGCCGTGCGCGCGCACCGTCTTAGACACTGCACTGCTGCATGCGGTGATCGCCGGCCACGAT
GCCCGCGACTCCACCTCCGTGGAAGCAGCGGTGCCCGATATCGTAGGTGCTGCTAAGGCCGGCGAGTCGGGTGACCTGCA
CGGCGTGCGGGTAGGGGTGGTCCGGCAGTTGCGTGGCGAGGGTTATCAGTCGGGGGTGTTGGCGTCATTTCAGGCCGCTG
TCGAGCAGTTGATCGCACTGGGTGCAACGGTCAGCGAAGTCGACTGCCCGCACTTTGACTACGCGCTGGCCGCCTACTAT
CTGATCTTGCCATCGGAGGTGTCAAGCAACCTCGCGCGTTTCGATGCGATGCGCTACGGGCTGCGGGTCGGTGACGACGG
CAGCCGCAGCGCCGAAGAGGTGATTGCGCTGACCAGGGCTGCCGGGTTCGGGCCGGAGGTCAAGCGGCGCATCATGATCG
GTGCCTACGCATTATCGGCCGGCTATTACGATTCCTACTACAGCCAGGCGCAGAAGGTGCGCACGTTGATTGCGCGTGAT
TTAGACAAAGCTTACCGATCCGTCGACGTGCTGGTGTCCCCGGCGACGCCGACTACCGCGTTCAGCCTTGGCGAAAAAGC
CGACGATCCGCTGGCGATGTACCTGTTCGATCTGTGCACGTTGCCACTCAACCTGGCTGGCCATTGCGGCATGTCGGTGC
CGTCCGGGCTGTCCTCGGATGACGATCTGCCGGTGGGCCTGCAGATCATGGCGCCCGCTTTGGCCGACGACCGACTCTAC
CGGGTGGGTGCCGCCTACGAGGCCGCACGCGGGCCATTGCCGTCCGCTATTTGA

Upstream 100 bases:

>100_bases
ACGAGACTGTCCCGTGTCTGACTCAGCAGCAGGCGCTGGCCGAAGCACCCGAAGCCATTTACGGCCGCTTTGTTGTCCCT
CAGATCTTGGGGGACAACAA

Downstream 100 bases:

>100_bases
GTTTCTTCGGTCACGAGCAGCTGCCAACGCGAGCAGATATTTGCGTTTGTTCGCGCTATCTGAGGCGTTCGCATGCAAGA
TGAAGGCATGAGGATCGGAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEATDDTYRAFLHVAAEKALSAAAAVDKAVAAGGQLSSTLAGV
PLALKDVFTTVDMPTTCGSKILQGWHSPYDATVTTRLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVDRVPGGS
GGGSAAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGHD
ARDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQSGVLASFQAAVEQLIALGATVSEVDCPHFDYALAAYY
LILPSEVSSNLARFDAMRYGLRVGDDGSRSAEEVIALTRAAGFGPEVKRRIMIGAYALSAGYYDSYYSQAQKVRTLIARD
LDKAYRSVDVLVSPATPTTAFSLGEKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSSDDDLPVGLQIMAPALADDRLY
RVGAAYEAARGPLPSAI

Sequences:

>Translated_497_residues
MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEATDDTYRAFLHVAAEKALSAAAAVDKAVAAGGQLSSTLAGV
PLALKDVFTTVDMPTTCGSKILQGWHSPYDATVTTRLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVDRVPGGS
GGGSAAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGHD
ARDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQSGVLASFQAAVEQLIALGATVSEVDCPHFDYALAAYY
LILPSEVSSNLARFDAMRYGLRVGDDGSRSAEEVIALTRAAGFGPEVKRRIMIGAYALSAGYYDSYYSQAQKVRTLIARD
LDKAYRSVDVLVSPATPTTAFSLGEKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSSDDDLPVGLQIMAPALADDRLY
RVGAAYEAARGPLPSAI
>Mature_497_residues
MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEATDDTYRAFLHVAAEKALSAAAAVDKAVAAGGQLSSTLAGV
PLALKDVFTTVDMPTTCGSKILQGWHSPYDATVTTRLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVDRVPGGS
GGGSAAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGHD
ARDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQSGVLASFQAAVEQLIALGATVSEVDCPHFDYALAAYY
LILPSEVSSNLARFDAMRYGLRVGDDGSRSAEEVIALTRAAGFGPEVKRRIMIGAYALSAGYYDSYYSQAQKVRTLIARD
LDKAYRSVDVLVSPATPTTAFSLGEKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSSDDDLPVGLQIMAPALADDRLY
RVGAAYEAARGPLPSAI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=36.2173038229376, Blast_Score=272, Evalue=6e-73,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=24.4532803180915, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=424, Percent_Identity=37.0283018867925, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17537465, Length=499, Percent_Identity=24.8496993987976, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=197, Percent_Identity=28.4263959390863, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=211, Percent_Identity=28.9099526066351, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=170, Percent_Identity=30, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=33.5731414868106, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=414, Percent_Identity=29.7101449275362, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=40.3470715835141, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=24.9003984063745, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=24.9003984063745, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=24.9003984063745, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=24.9003984063745, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=521, Percent_Identity=26.679462571977, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550774, Length=208, Percent_Identity=29.8076923076923, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648435, Length=208, Percent_Identity=29.8076923076923, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648437, Length=208, Percent_Identity=29.8076923076923, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356731, Length=201, Percent_Identity=29.8507462686567, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI161078093, Length=524, Percent_Identity=25, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648441, Length=132, Percent_Identity=35.6060606060606, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648439, Length=132, Percent_Identity=35.6060606060606, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_MYCLE (O33105)

Other databases:

- EMBL:   Z99263
- EMBL:   AL583923
- PIR:   T45406
- RefSeq:   NP_302171.1
- ProteinModelPortal:   O33105
- SMR:   O33105
- EnsemblBacteria:   EBMYCT00000027935
- GeneID:   910792
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1702
- NMPDR:   fig|272631.1.peg.1043
- Leproma:   ML1702
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   MLEP272631:ML1702-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51537; Mature: 51537

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 85-85 ACT_SITE 160-160 ACT_SITE 184-184

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEATDDTYRAFLHVAAEKALSAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAVDKAVAAGGQLSSTLAGVPLALKDVFTTVDMPTTCGSKILQGWHSPYDATVTTRLRAA
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHC
GIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVDRVPGGSGGGSAAALAAFQAPLAIGSD
CCCEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCC
TGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGHD
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
ARDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQSGVLASFQAAVEQLIAL
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GATVSEVDCPHFDYALAAYYLILPSEVSSNLARFDAMRYGLRVGDDGSRSAEEVIALTRA
CCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHH
AGFGPEVKRRIMIGAYALSAGYYDSYYSQAQKVRTLIARDLDKAYRSVDVLVSPATPTTA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHH
FSLGEKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSSDDDLPVGLQIMAPALADDRLY
HHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHH
RVGAAYEAARGPLPSAI
HHCCHHHHHCCCCCCCC
>Mature Secondary Structure
MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEATDDTYRAFLHVAAEKALSAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAVDKAVAAGGQLSSTLAGVPLALKDVFTTVDMPTTCGSKILQGWHSPYDATVTTRLRAA
HHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHC
GIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVDRVPGGSGGGSAAALAAFQAPLAIGSD
CCCEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCC
TGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGHD
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
ARDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQSGVLASFQAAVEQLIAL
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GATVSEVDCPHFDYALAAYYLILPSEVSSNLARFDAMRYGLRVGDDGSRSAEEVIALTRA
CCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHH
AGFGPEVKRRIMIGAYALSAGYYDSYYSQAQKVRTLIARDLDKAYRSVDVLVSPATPTTA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHH
FSLGEKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSSDDDLPVGLQIMAPALADDRLY
HHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHH
RVGAAYEAARGPLPSAI
HHCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002