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The map label for this gene is pfkA [H]

Identifier: 221230384

GI number: 221230384

Start: 2051925

End: 2052956

Strand: Reverse

Name: pfkA [H]

Synonym: MLBr_01701

Alternate gene names: 221230384

Gene position: 2052956-2051925 (Counterclockwise)

Preceding gene: 221230385

Following gene: 221230383

Centisome position: 62.82

GC content: 62.31

Gene sequence:

>1032_bases
ATGAGGATCGGAATTCTCACGGGGGGCGGCGACTGTCCTGGTCTTAACGCCGTTATCCGGGCGATTGTGCGCACCTGTGA
CGCCCGGTACGGTTCGTCGGTAGTCGGGTTTCAGGACGGCTGGCGTGGGTTGCTGGAGAATCGGCGCATGCAGCTGTGCA
ACGACGACCGGAATGACCGTCTGTTAGCCAAAGGCGGAACCATGCTGGGCACTGCCCACGTGCACCCCGACAAGCTGCGG
GCCGGGCTGCATCAGATCAAGCAGACCCTGGATGACAATGGCATCGACGTGCTCATCCCGATCGGCGGCGAAGGCACGCT
GACGGCCGCGCACTGGCTGTCGCAGGAAGACGTTCCTGTGGTCGGCGTGCCGAAGACGATCGACAACGACATCGACTGCA
CCGACGTGACCTTTGGCCACGACACCGCGCTGACGGTGGCTACCGAAGCCATTGACCGGTTGCACAGTACCGCCGAATCT
CACCAACGTGTGATGCTGGTGGAGGTGATGGGTCGGCATGCCGGCTGGATAGCGCTGAGTTCCGGGCTGGCTTCCGGTGC
GCACATGACGCTGATTCCCGAACAGCCCTTCGATGTCGAGGAAGTGTGCTGCCTCGTGAAACGACGTTTTCAGCGCGGGG
ATTCGCATTTCATTTGCGTGGTCGCTGAGGGTGCCAAGCCGGTCCCAGGCTCGATCACGTTGCGCCAAGGTGGGATGGAT
GAATTTGGTCACGAGCGTTTCACTGGGGTGGCAGCGCAATTGGGCGCTGAGGTCGAGAAGCGGATCAACAAGGACGTGCG
AGTGACTGTGCTGGGTCATGTCCAACGCGGTGGTACACCGACGGCCTTCGATCGGGTGCTGGCCACCCGGTTCGGGGTTA
ATGCTGCCGACGCCTCGCACGCCGGCGAGTACGGCCAGATGGTGTCACTGCGTGGGCAAGACATCGGTCGCGTACCGCTG
GAGGATGCGGTGCGTCAGCTCAAATTGGTGCCCGAAAGCCGTTACGACGACGCCGCCGCCTTCTTCGGTTAA

Upstream 100 bases:

>100_bases
GTCCGCTATTTGAGTTTCTTCGGTCACGAGCAGCTGCCAACGCGAGCAGATATTTGCGTTTGTTCGCGCTATCTGAGGCG
TTCGCATGCAAGATGAAGGC

Downstream 100 bases:

>100_bases
GATGGGAATGCTACTGCAAATACCGCGGTAGCGTCAACTGTTCGACTGTCTGAATTTCGAGGTGGCCATATGCACCAAAC
GCTGGCAAGACACTCACGTC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRIGILTGGGDCPGLNAVIRAIVRTCDARYGSSVVGFQDGWRGLLENRRMQLCNDDRNDRLLAKGGTMLGTAHVHPDKLR
AGLHQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSQEDVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
HQRVMLVEVMGRHAGWIALSSGLASGAHMTLIPEQPFDVEEVCCLVKRRFQRGDSHFICVVAEGAKPVPGSITLRQGGMD
EFGHERFTGVAAQLGAEVEKRINKDVRVTVLGHVQRGGTPTAFDRVLATRFGVNAADASHAGEYGQMVSLRGQDIGRVPL
EDAVRQLKLVPESRYDDAAAFFG

Sequences:

>Translated_343_residues
MRIGILTGGGDCPGLNAVIRAIVRTCDARYGSSVVGFQDGWRGLLENRRMQLCNDDRNDRLLAKGGTMLGTAHVHPDKLR
AGLHQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSQEDVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
HQRVMLVEVMGRHAGWIALSSGLASGAHMTLIPEQPFDVEEVCCLVKRRFQRGDSHFICVVAEGAKPVPGSITLRQGGMD
EFGHERFTGVAAQLGAEVEKRINKDVRVTVLGHVQRGGTPTAFDRVLATRFGVNAADASHAGEYGQMVSLRGQDIGRVPL
EDAVRQLKLVPESRYDDAAAFFG
>Mature_343_residues
MRIGILTGGGDCPGLNAVIRAIVRTCDARYGSSVVGFQDGWRGLLENRRMQLCNDDRNDRLLAKGGTMLGTAHVHPDKLR
AGLHQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSQEDVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
HQRVMLVEVMGRHAGWIALSSGLASGAHMTLIPEQPFDVEEVCCLVKRRFQRGDSHFICVVAEGAKPVPGSITLRQGGMD
EFGHERFTGVAAQLGAEVEKRINKDVRVTVLGHVQRGGTPTAFDRVLATRFGVNAADASHAGEYGQMVSLRGQDIGRVPL
EDAVRQLKLVPESRYDDAAAFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=380, Percent_Identity=34.7368421052632, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI48762920, Length=375, Percent_Identity=34.1333333333333, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI266453768, Length=375, Percent_Identity=35.4666666666667, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI266453748, Length=375, Percent_Identity=35.4666666666667, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4505749, Length=375, Percent_Identity=35.4666666666667, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI266453619, Length=375, Percent_Identity=35.4666666666667, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1790350, Length=328, Percent_Identity=37.5, Blast_Score=208, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI25147584, Length=366, Percent_Identity=33.879781420765, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558788, Length=380, Percent_Identity=30.5263157894737, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI72003601, Length=353, Percent_Identity=25.2124645892351, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321679, Length=367, Percent_Identity=34.0599455040872, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323861, Length=373, Percent_Identity=34.3163538873995, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28573326, Length=375, Percent_Identity=34.6666666666667, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24652337, Length=375, Percent_Identity=34.9333333333333, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI17647809, Length=375, Percent_Identity=34.9333333333333, Blast_Score=181, Evalue=5e-46,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37002; Mature: 37002

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGILTGGGDCPGLNAVIRAIVRTCDARYGSSVVGFQDGWRGLLENRRMQLCNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCE
LLAKGGTMLGTAHVHPDKLRAGLHQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSQEDVPV
EEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCE
VGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAESHQRVMLVEVMGRHAGWIALS
EECCHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCEEEEE
SGLASGAHMTLIPEQPFDVEEVCCLVKRRFQRGDSHFICVVAEGAKPVPGSITLRQGGMD
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCC
EFGHERFTGVAAQLGAEVEKRINKDVRVTVLGHVQRGGTPTAFDRVLATRFGVNAADASH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCC
AGEYGQMVSLRGQDIGRVPLEDAVRQLKLVPESRYDDAAAFFG
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MRIGILTGGGDCPGLNAVIRAIVRTCDARYGSSVVGFQDGWRGLLENRRMQLCNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCE
LLAKGGTMLGTAHVHPDKLRAGLHQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSQEDVPV
EEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCE
VGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAESHQRVMLVEVMGRHAGWIALS
EECCHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCEEEEE
SGLASGAHMTLIPEQPFDVEEVCCLVKRRFQRGDSHFICVVAEGAKPVPGSITLRQGGMD
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCC
EFGHERFTGVAAQLGAEVEKRINKDVRVTVLGHVQRGGTPTAFDRVLATRFGVNAADASH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCC
AGEYGQMVSLRGQDIGRVPLEDAVRQLKLVPESRYDDAAAFFG
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]