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The map label for this gene is gatB

Identifier: 221230383

GI number: 221230383

Start: 2050047

End: 2051576

Strand: Reverse

Name: gatB

Synonym: MLBr_01700

Alternate gene names: 221230383

Gene position: 2051576-2050047 (Counterclockwise)

Preceding gene: 221230384

Following gene: 221230381

Centisome position: 62.78

GC content: 58.82

Gene sequence:

>1530_bases
ATGACTGTTGTTTCCGGGGCGTCCAAGGCTTCAGGGGCGGATTTGCTGGATTATGATGTTGTAGTCGCGCGGTTTGATCC
GGTGTTCGGGCTTGAGGTGCATGTTGAGCTGTCCACTGTGACTAAAATGTTCTGTGGCTGTGCCACCACATTTGGCGCTG
AACCAAACACTCAGGTGTGCCCGGTGTGTCTGGGCCTGCCGGGTTCGCTGCCAGTGCTCAACCGGGCCGCGGTCCAGTCG
GCGATTCGGATCGGGCTGGCGTTGAACTGTGAAATCGTGCCCTGGTGCCGCTTCGCACGGAAGAACTACTTCTACCCAGA
CGTGCCTAAGAATTACCAAATATCGCAATACGATGAGCCGATCGCCATTAACGGCTACCTCGAGGTACCTCTGGAAGACG
ACACTACCTGGCGGGTGGAGATCGAACGGGCGCACATGGAGGAAGACACCGGCAAGCTGACCCACCTAGGCAGCGAGACC
GGCCGCATTTACGGCGCAACGACGTCGCTGATTGACTACAACCGTGCTGGCGTGCCGTTGATCGAAATCGTCACCAAGCC
TATTGAGGGGGCCGGAGTTCGGGCACCACAGATCGCTCGGGCGTATGTGAAGGCATTGCAAGATCTGTTGCGCACGTTGG
ACGTATCCGATGTCCGGATGGACCAAGGCTCAATGCGTTGTGACGCCAACGTATCGTTGAAACCTATCGGTACGGTCGAA
TTCGGCACCAGGAGCGAGATCAAAAACGTTAATTCATTGAAAAGTGTTGAAATGGCTGTACGCTACGAGATGCAGCGCCA
AGGAGCCATTCTGGTGTCCGGAGGTCGAATCGCCCAGGAGACCAGGCACTTTCATGAGGACGGGTACACTAGCCCGGGAC
GCGCCAAGGAGACAGCGCAGGACTACCGATATTTCCCCGACCCCGACCTAGAGCCCGTCGCGCCCAGCCGCGAGCTGGTC
GAGCAATTGCGTCAAACAATACCGGAGTTACCGTGGTTGAGCCGCAAGAGGATTCAGCAGGAATGGGGTATTTCCGACGA
GGTGATGCGAGATCTCGTCAACGCTGGCGCGGTTGAATTGGTCGCCGCGACCGTCAAGAACGGCGCCTCCAGCGAACAAG
CACGCGCTTGGTGGGGAAACTTCTTGGTGCAGAAAGCTAATGAGGCAAACATCACCTTGGACGAGTTGGCCATCACTCCT
GCCCAAGTCGCCGTGGTAGTGGCGTTGGTCGACGAGGGTAAGCTGTCCATTAGGTTGGCTCGCCAGGTCGTCGAGGGTGT
GCTTGCCGGGGAGGGTGAACCCGAGCAGGTTATGGTTGATCGCGACTTGGCCTTGGTGCGCGACGACTCGGTGATGCAGG
CTGCTGTCGACGAGGCTTTGGCCGCCGATCCTGATGTGGCGGAAAAGATTCGTGGCGGCAAGGTGGCGGCGGCCGGTGCG
ATCGTAGGAGCGGTGATGAAGACCACCCGCGGGCAGGCCGATGCTGCCCGGGTGCGTGAACTCGTCTTGGCCATTTGCGG
GCAGGGGTAA

Upstream 100 bases:

>100_bases
AGCATGACCTTCGCATAGCGGTCTCTCGGCCGGGCGCCTAGGTGGTGGCCCGCTTAGTTCGGCTCGGCTGGTCTCGCAAT
CGGCCTCTAGGATCGAGCTC

Downstream 100 bases:

>100_bases
CTGAACAGTTTAAGTAGACCAGAAGCTGTTGTGAAGCTGAGGCGATTGTGTAACGATTAGCATGACGTCTTCTATACTTT
AAATATACTTTACTTTATAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTVVSGASKASGADLLDYDVVVARFDPVFGLEVHVELSTVTKMFCGCATTFGAEPNTQVCPVCLGLPGSLPVLNRAAVQS
AIRIGLALNCEIVPWCRFARKNYFYPDVPKNYQISQYDEPIAINGYLEVPLEDDTTWRVEIERAHMEEDTGKLTHLGSET
GRIYGATTSLIDYNRAGVPLIEIVTKPIEGAGVRAPQIARAYVKALQDLLRTLDVSDVRMDQGSMRCDANVSLKPIGTVE
FGTRSEIKNVNSLKSVEMAVRYEMQRQGAILVSGGRIAQETRHFHEDGYTSPGRAKETAQDYRYFPDPDLEPVAPSRELV
EQLRQTIPELPWLSRKRIQQEWGISDEVMRDLVNAGAVELVAATVKNGASSEQARAWWGNFLVQKANEANITLDELAITP
AQVAVVVALVDEGKLSIRLARQVVEGVLAGEGEPEQVMVDRDLALVRDDSVMQAAVDEALAADPDVAEKIRGGKVAAAGA
IVGAVMKTTRGQADAARVRELVLAICGQG

Sequences:

>Translated_509_residues
MTVVSGASKASGADLLDYDVVVARFDPVFGLEVHVELSTVTKMFCGCATTFGAEPNTQVCPVCLGLPGSLPVLNRAAVQS
AIRIGLALNCEIVPWCRFARKNYFYPDVPKNYQISQYDEPIAINGYLEVPLEDDTTWRVEIERAHMEEDTGKLTHLGSET
GRIYGATTSLIDYNRAGVPLIEIVTKPIEGAGVRAPQIARAYVKALQDLLRTLDVSDVRMDQGSMRCDANVSLKPIGTVE
FGTRSEIKNVNSLKSVEMAVRYEMQRQGAILVSGGRIAQETRHFHEDGYTSPGRAKETAQDYRYFPDPDLEPVAPSRELV
EQLRQTIPELPWLSRKRIQQEWGISDEVMRDLVNAGAVELVAATVKNGASSEQARAWWGNFLVQKANEANITLDELAITP
AQVAVVVALVDEGKLSIRLARQVVEGVLAGEGEPEQVMVDRDLALVRDDSVMQAAVDEALAADPDVAEKIRGGKVAAAGA
IVGAVMKTTRGQADAARVRELVLAICGQG
>Mature_508_residues
TVVSGASKASGADLLDYDVVVARFDPVFGLEVHVELSTVTKMFCGCATTFGAEPNTQVCPVCLGLPGSLPVLNRAAVQSA
IRIGLALNCEIVPWCRFARKNYFYPDVPKNYQISQYDEPIAINGYLEVPLEDDTTWRVEIERAHMEEDTGKLTHLGSETG
RIYGATTSLIDYNRAGVPLIEIVTKPIEGAGVRAPQIARAYVKALQDLLRTLDVSDVRMDQGSMRCDANVSLKPIGTVEF
GTRSEIKNVNSLKSVEMAVRYEMQRQGAILVSGGRIAQETRHFHEDGYTSPGRAKETAQDYRYFPDPDLEPVAPSRELVE
QLRQTIPELPWLSRKRIQQEWGISDEVMRDLVNAGAVELVAATVKNGASSEQARAWWGNFLVQKANEANITLDELAITPA
QVAVVVALVDEGKLSIRLARQVVEGVLAGEGEPEQVMVDRDLALVRDDSVMQAAVDEALAADPDVAEKIRGGKVAAAGAI
VGAVMKTTRGQADAARVRELVLAICGQG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=34.6, Blast_Score=268, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI115534094, Length=293, Percent_Identity=41.296928327645, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=316, Percent_Identity=34.4936708860759, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=29.8989898989899, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_MYCLB (B8ZS22)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503799.1
- ProteinModelPortal:   B8ZS22
- SMR:   B8ZS22
- EnsemblBacteria:   EBMYCT00000087228
- GeneID:   7326797
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017594
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55362; Mature: 55230

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01234 GATB; PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVSGASKASGADLLDYDVVVARFDPVFGLEVHVELSTVTKMFCGCATTFGAEPNTQVC
CCEECCCCCCCCCCCCHHEEEEEECCCEECEEEEEEHHHHHHHHHHHHHHCCCCCCCCCC
PVCLGLPGSLPVLNRAAVQSAIRIGLALNCEIVPWCRFARKNYFYPDVPKNYQISQYDEP
EEEECCCCCCCHHHHHHHHHHHHHCEEECCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCC
IAINGYLEVPLEDDTTWRVEIERAHMEEDTGKLTHLGSETGRIYGATTSLIDYNRAGVPL
EEEECEEEECCCCCCEEEEEEEEHHCCCCCCCEEECCCCCCEEEEEEHHHEECCCCCCCH
IEIVTKPIEGAGVRAPQIARAYVKALQDLLRTLDVSDVRMDQGSMRCDANVSLKPIGTVE
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCEECCCCEEC
FGTRSEIKNVNSLKSVEMAVRYEMQRQGAILVSGGRIAQETRHFHEDGYTSPGRAKETAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHH
DYRYFPDPDLEPVAPSRELVEQLRQTIPELPWLSRKRIQQEWGISDEVMRDLVNAGAVEL
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
VAATVKNGASSEQARAWWGNFLVQKANEANITLDELAITPAQVAVVVALVDEGKLSIRLA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHHEEEEEEECCCCHHHHHH
RQVVEGVLAGEGEPEQVMVDRDLALVRDDSVMQAAVDEALAADPDVAEKIRGGKVAAAGA
HHHHHHHHCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHH
IVGAVMKTTRGQADAARVRELVLAICGQG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVVSGASKASGADLLDYDVVVARFDPVFGLEVHVELSTVTKMFCGCATTFGAEPNTQVC
CEECCCCCCCCCCCCHHEEEEEECCCEECEEEEEEHHHHHHHHHHHHHHCCCCCCCCCC
PVCLGLPGSLPVLNRAAVQSAIRIGLALNCEIVPWCRFARKNYFYPDVPKNYQISQYDEP
EEEECCCCCCCHHHHHHHHHHHHHCEEECCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCC
IAINGYLEVPLEDDTTWRVEIERAHMEEDTGKLTHLGSETGRIYGATTSLIDYNRAGVPL
EEEECEEEECCCCCCEEEEEEEEHHCCCCCCCEEECCCCCCEEEEEEHHHEECCCCCCCH
IEIVTKPIEGAGVRAPQIARAYVKALQDLLRTLDVSDVRMDQGSMRCDANVSLKPIGTVE
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCEECCCCEEC
FGTRSEIKNVNSLKSVEMAVRYEMQRQGAILVSGGRIAQETRHFHEDGYTSPGRAKETAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHH
DYRYFPDPDLEPVAPSRELVEQLRQTIPELPWLSRKRIQQEWGISDEVMRDLVNAGAVEL
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
VAATVKNGASSEQARAWWGNFLVQKANEANITLDELAITPAQVAVVVALVDEGKLSIRLA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHHEEEEEEECCCCHHHHHH
RQVVEGVLAGEGEPEQVMVDRDLALVRDDSVMQAAVDEALAADPDVAEKIRGGKVAAAGA
HHHHHHHHCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHH
IVGAVMKTTRGQADAARVRELVLAICGQG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA