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The map label for this gene is serA

Identifier: 221230377

GI number: 221230377

Start: 2038142

End: 2039728

Strand: Reverse

Name: serA

Synonym: MLBr_01692

Alternate gene names: 221230377

Gene position: 2039728-2038142 (Counterclockwise)

Preceding gene: 221230378

Following gene: 221230376

Centisome position: 62.41

GC content: 62.0

Gene sequence:

>1587_bases
GTGGACCTGCCTGTTGTGTTAATTGCCGACAAACTCGCCCAATCAACCGTGGCTGCCCTGGGAGACCAAGTCGAGGTGCG
GTGGGTGGACGGTCCAGACCGGACGAAGCTGTTAGCTGCAGTACCCGAGGCCGACGCGTTGTTGGTGCGGTCGGCCACTA
CTGTCGACGCCGAGGTGCTGGCAGCCGCTCCTAAGCTCAAGATCGTCGCCCGTGCCGGGGTAGGGCTAGACAACGTTGAT
GTCGATGCCGCCACCGCGCGCGGTGTCCTGGTAGTCAACGCCCCAACGTCGAACATTCACAGCGCCGCTGAGCACGCGTT
GGCGCTGCTATTGGCAGCTTCTCGGCAGATCGCGGAGGCCGACGCCTCACTGCGTGCACACATCTGGAAACGGTCGTCGT
TCTCCGGCACCGAAATTTTCGGCAAGACCGTCGGCGTGGTGGGGCTGGGTCGGATTGGGCAGTTGGTTGCCGCACGGATA
GCAGCGTTCGGGGCTCACGTTATCGCTTACGACCCGTATGTGGCGCCGGCACGGGCCGCGCAGCTTGGTATCGAGCTGAT
GTCTTTTGACGATCTCCTAGCCCGGGCCGATTTTATCTCAGTGCATTTGCCGAAGACGCCCGAGACGGCGGGCCTGATCG
ACAAGGAGGCGCTGGCCAAAACCAAGCCCGGTGTCATCATTGTCAATGCCGCACGCGGCGGCTTAGTGGACGAGGTGGCG
CTAGCCGATGCGGTGCGCAGCGGACATGTTCGGGCGGCCGGTCTAGATGTGTTTGCCACCGAACCGTGCACCGATAGCCC
GCTGTTTGAACTATCGCAGGTGGTGGTGACACCGCATCTGGGGGCGTCTACCGCCGAAGCCCAGGATCGAGCAGGTACTG
ATGTGGCCGAAAGCGTGCGGCTGGCGCTGGCGGGGGAGTTTGTGCCTGACGCGGTCAACGTGGACGGGGGCGTGGTCAAC
GAAGAGGTGGCTCCCTGGCTGGACTTGGTGTGCAAGCTTGGGGTGCTGGTAGCCGCGTTATCCGATGAACTGCCGGCGTC
GTTGTCGGTGCACGTGCGTGGCGAGTTGGCTTCTGAAGACGTTGAAATATTGCGGCTTTCGGCCCTACGTGGGCTTTTCT
CGACGGTCATAGAGGATGCTGTGACGTTCGTCAACGCACCGGCACTGGCCGCCGAACGAGGTGTGTCCGCTGAAATCACT
ACGGGCTCGGAGAGCCCCAACCATCGCAGTGTGGTCGACGTGCGGGCGGTCGCCTCCGACGGCTCGGTGGTCAACATAGC
CGGTACGTTGTCTGGGCCGCAACTGGTGCAGAAGATCGTGCAGGTCAATGGTCGTAACTTTGATTTGCGTGCGCAGGGCA
TGAACTTGGTGATCAGGTATGTCGACCAACCTGGCGCTCTGGGCAAGATTGGCACTTTGCTGGGCGCGGCCGGGGTGAAT
ATCCAAGCTGCTCAGCTGTCTGAGGACACCGAGGGGCCAGGTGCGACGATTCTGTTGAGGCTGGATCAAGACGTGCCGGG
TGATGTGCGGTCGGCGATCGTGGCAGCGGTGAGTGCCAACAAGCTTGAGGTAGTCAATCTGTCATGA

Upstream 100 bases:

>100_bases
GCGTCCTGCGCGGGCGCAGGTCAGGCAGCAGAGATCTTGGTGAGTTCGCTGTCGACGACGGTGACGTCCACATGTCAATT
ACAGTCGATACTCTGGCGGC

Downstream 100 bases:

>100_bases
CTTACTCCGGTGACGATCCAGCGCGAGGCGATGAGAAGGAGCGGGGCAGATGAAATTGGCGATCATCGGTGGCGACGGGA
TCGGCCCGGAGGTGGTCGCA

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: PGDH

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MDLPVVLIADKLAQSTVAALGDQVEVRWVDGPDRTKLLAAVPEADALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVD
VDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIAEADASLRAHIWKRSSFSGTEIFGKTVGVVGLGRIGQLVAARI
AAFGAHVIAYDPYVAPARAAQLGIELMSFDDLLARADFISVHLPKTPETAGLIDKEALAKTKPGVIIVNAARGGLVDEVA
LADAVRSGHVRAAGLDVFATEPCTDSPLFELSQVVVTPHLGASTAEAQDRAGTDVAESVRLALAGEFVPDAVNVDGGVVN
EEVAPWLDLVCKLGVLVAALSDELPASLSVHVRGELASEDVEILRLSALRGLFSTVIEDAVTFVNAPALAAERGVSAEIT
TGSESPNHRSVVDVRAVASDGSVVNIAGTLSGPQLVQKIVQVNGRNFDLRAQGMNLVIRYVDQPGALGKIGTLLGAAGVN
IQAAQLSEDTEGPGATILLRLDQDVPGDVRSAIVAAVSANKLEVVNLS

Sequences:

>Translated_528_residues
MDLPVVLIADKLAQSTVAALGDQVEVRWVDGPDRTKLLAAVPEADALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVD
VDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIAEADASLRAHIWKRSSFSGTEIFGKTVGVVGLGRIGQLVAARI
AAFGAHVIAYDPYVAPARAAQLGIELMSFDDLLARADFISVHLPKTPETAGLIDKEALAKTKPGVIIVNAARGGLVDEVA
LADAVRSGHVRAAGLDVFATEPCTDSPLFELSQVVVTPHLGASTAEAQDRAGTDVAESVRLALAGEFVPDAVNVDGGVVN
EEVAPWLDLVCKLGVLVAALSDELPASLSVHVRGELASEDVEILRLSALRGLFSTVIEDAVTFVNAPALAAERGVSAEIT
TGSESPNHRSVVDVRAVASDGSVVNIAGTLSGPQLVQKIVQVNGRNFDLRAQGMNLVIRYVDQPGALGKIGTLLGAAGVN
IQAAQLSEDTEGPGATILLRLDQDVPGDVRSAIVAAVSANKLEVVNLS
>Mature_528_residues
MDLPVVLIADKLAQSTVAALGDQVEVRWVDGPDRTKLLAAVPEADALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVD
VDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIAEADASLRAHIWKRSSFSGTEIFGKTVGVVGLGRIGQLVAARI
AAFGAHVIAYDPYVAPARAAQLGIELMSFDDLLARADFISVHLPKTPETAGLIDKEALAKTKPGVIIVNAARGGLVDEVA
LADAVRSGHVRAAGLDVFATEPCTDSPLFELSQVVVTPHLGASTAEAQDRAGTDVAESVRLALAGEFVPDAVNVDGGVVN
EEVAPWLDLVCKLGVLVAALSDELPASLSVHVRGELASEDVEILRLSALRGLFSTVIEDAVTFVNAPALAAERGVSAEIT
TGSESPNHRSVVDVRAVASDGSVVNIAGTLSGPQLVQKIVQVNGRNFDLRAQGMNLVIRYVDQPGALGKIGTLLGAAGVN
IQAAQLSEDTEGPGATILLRLDQDVPGDVRSAIVAAVSANKLEVVNLS

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Homo sapiens, GI23308577, Length=409, Percent_Identity=38.6308068459658, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI4557497, Length=281, Percent_Identity=33.0960854092527, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI61743967, Length=281, Percent_Identity=33.0960854092527, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI145580578, Length=298, Percent_Identity=32.5503355704698, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI4557499, Length=298, Percent_Identity=32.5503355704698, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI6912396, Length=277, Percent_Identity=33.9350180505415, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI145580575, Length=298, Percent_Identity=32.5503355704698, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1789279, Length=269, Percent_Identity=35.6877323420074, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI87082289, Length=319, Percent_Identity=35.7366771159875, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1787645, Length=196, Percent_Identity=35.2040816326531, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788660, Length=275, Percent_Identity=32.7272727272727, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081824, Length=234, Percent_Identity=27.3504273504274, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=303, Percent_Identity=46.2046204620462, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI25147481, Length=291, Percent_Identity=32.3024054982818, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320925, Length=307, Percent_Identity=31.5960912052117, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6322116, Length=289, Percent_Identity=31.1418685121107, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324055, Length=278, Percent_Identity=34.8920863309352, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324964, Length=271, Percent_Identity=28.0442804428044, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6325144, Length=152, Percent_Identity=33.5526315789474, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=157, Percent_Identity=31.2101910828025, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=200, Percent_Identity=25, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=327, Percent_Identity=42.2018348623853, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI28571528, Length=279, Percent_Identity=38.7096774193548, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24646446, Length=299, Percent_Identity=33.7792642140468, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24646448, Length=299, Percent_Identity=33.7792642140468, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24646452, Length=299, Percent_Identity=33.7792642140468, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24646450, Length=299, Percent_Identity=33.7792642140468, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI62472511, Length=299, Percent_Identity=33.7792642140468, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574286, Length=300, Percent_Identity=31.6666666666667, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45552429, Length=281, Percent_Identity=32.3843416370107, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28574284, Length=281, Percent_Identity=32.3843416370107, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24585514, Length=281, Percent_Identity=32.3843416370107, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28574282, Length=281, Percent_Identity=32.3843416370107, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45551003, Length=281, Percent_Identity=32.3843416370107, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24585516, Length=280, Percent_Identity=30.7142857142857, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SERA_MYCLE (O33116)

Other databases:

- EMBL:   Z99263
- EMBL:   AL583923
- PIR:   T45418
- RefSeq:   NP_302163.1
- ProteinModelPortal:   O33116
- SMR:   O33116
- EnsemblBacteria:   EBMYCT00000029282
- GeneID:   910062
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1692
- NMPDR:   fig|272631.1.peg.1035
- Leproma:   ML1692
- GeneTree:   EBGT00050000016363
- HOGENOM:   HBG731446
- OMA:   KHAVMAI
- ProtClustDB:   PRK13581
- BioCyc:   MLEP272631:ML1692-MONOMER
- BRENDA:   1.1.1.95
- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF20
- TIGRFAMs:   TIGR01327

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT

EC number: =1.1.1.95

Molecular weight: Translated: 54470; Mature: 54470

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 232-232 ACT_SITE 261-261 ACT_SITE 279-279 BINDING 171-171 BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPVVLIADKLAQSTVAALGDQVEVRWVDGPDRTKLLAAVPEADALLVRSATTVDAEVL
CCCCEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCHHHH
AAAPKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIAEA
HCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DASLRAHIWKRSSFSGTEIFGKTVGVVGLGRIGQLVAARIAAFGAHVIAYDPYVAPARAA
HHHHHHHHEECCCCCCCHHHHHHHCEEEHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHH
QLGIELMSFDDLLARADFISVHLPKTPETAGLIDKEALAKTKPGVIIVNAARGGLVDEVA
HHHHHHCCHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCHHHHH
LADAVRSGHVRAAGLDVFATEPCTDSPLFELSQVVVTPHLGASTAEAQDRAGTDVAESVR
HHHHHHCCCEEEECCEEEECCCCCCCCHHHHHHEEEECCCCCCCCCHHHCCCCCHHHHHH
LALAGEFVPDAVNVDGGVVNEEVAPWLDLVCKLGVLVAALSDELPASLSVHVRGELASED
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
VEILRLSALRGLFSTVIEDAVTFVNAPALAAERGVSAEITTGSESPNHRSVVDVRAVASD
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCEEEEEEEECC
GSVVNIAGTLSGPQLVQKIVQVNGRNFDLRAQGMNLVIRYVDQPGALGKIGTLLGAAGVN
CCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHCCCC
IQAAQLSEDTEGPGATILLRLDQDVPGDVRSAIVAAVSANKLEVVNLS
EEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MDLPVVLIADKLAQSTVAALGDQVEVRWVDGPDRTKLLAAVPEADALLVRSATTVDAEVL
CCCCEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCHHHH
AAAPKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIAEA
HCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DASLRAHIWKRSSFSGTEIFGKTVGVVGLGRIGQLVAARIAAFGAHVIAYDPYVAPARAA
HHHHHHHHEECCCCCCCHHHHHHHCEEEHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHH
QLGIELMSFDDLLARADFISVHLPKTPETAGLIDKEALAKTKPGVIIVNAARGGLVDEVA
HHHHHHCCHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCHHHHH
LADAVRSGHVRAAGLDVFATEPCTDSPLFELSQVVVTPHLGASTAEAQDRAGTDVAESVR
HHHHHHCCCEEEECCEEEECCCCCCCCHHHHHHEEEECCCCCCCCCHHHCCCCCHHHHHH
LALAGEFVPDAVNVDGGVVNEEVAPWLDLVCKLGVLVAALSDELPASLSVHVRGELASED
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
VEILRLSALRGLFSTVIEDAVTFVNAPALAAERGVSAEITTGSESPNHRSVVDVRAVASD
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCEEEEEEEECC
GSVVNIAGTLSGPQLVQKIVQVNGRNFDLRAQGMNLVIRYVDQPGALGKIGTLLGAAGVN
CCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHCCCC
IQAAQLSEDTEGPGATILLRLDQDVPGDVRSAIVAAVSANKLEVVNLS
EEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002