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The map label for this gene is leuB

Identifier: 221230376

GI number: 221230376

Start: 2037082

End: 2038092

Strand: Reverse

Name: leuB

Synonym: MLBr_01691

Alternate gene names: 221230376

Gene position: 2038092-2037082 (Counterclockwise)

Preceding gene: 221230377

Following gene: 221230375

Centisome position: 62.36

GC content: 59.05

Gene sequence:

>1011_bases
ATGAAATTGGCGATCATCGGTGGCGACGGGATCGGCCCGGAGGTGGTCGCACAGGCAGTCAAAATTCTTGATGTGGTGCT
GCCGGGCGTACAGAAGACTACCTACGATCTGGGCGCCCGGCGTTATCACACCACTGGTGAGCTATTGCCGGAGTCGGTGC
TCGCCGAGCTACGGGAGCACGACGCCATCTTGCTCGGGGCGGTAGGTGATCCCTCAGTCCCTAGTGGTGTGTTGGAGCGG
GGCTTGCTGCTGAGATTGCGCTTCGAGCTGGATCATCATATCAATCTGCGACCGGCCCGGTTGTATCCAGGTGTTAACAG
TTTGCTTGCGGGAAAGCCCGATATTGACTTTGTGGTGGTTCGCGAGGGCACCGAAGGCCCCTACACTGGTACCGGCGGTG
CGATCCGTGTCGGAACCCCTAACGAGGTGGCCACGGAGGTAAGTGTGAATACCGCTTTTGGTGTGCGCCGGGTGGTGCAA
GATGCGTTCGAACGTGCCCGACAGCGCCGCAAGCATCTGACGCTGGTACACAAGAACAACGTGCTGACCTATGCCGGGAC
GTTGTGGTGTAGGATCGTGCAGGAGGTCGGTGAAAAATACCCCGACGTCGAGGTTGTCTACCAGCATATAGATGCGGCCA
CCATCTACTTGGTCACCGAACCCAGTCGCTTTGATGTCATTGTCACTGATAACCTATTCGGTGATATCATCACCGACTTG
GCCGCAGCGGTGTGTGGTGGCATTGCCTTGGCCGCCAGTGGAAATATCGATGCCACCCGGACCAATCCGTCGATGTTCGA
GCCGGTGCATGGCAGCGCTCCGGATATTGCCGGCCAGGGCATCGCTGATCCGACCGCGGCGATAATGTCGTTGGCACTGC
TGCTCGCCCATCTCGGTGAGGATGAGCCGGCTGCTCGGTTGGACCAGGCGGTAGCGAGTTATTTGGCGACTCGCGGGAAC
GGGCGGTTTTCCACCGGCGAAGTTGGAGAACGAATAGCCGCTGCACTCTAG

Upstream 100 bases:

>100_bases
ATCGTGGCAGCGGTGAGTGCCAACAAGCTTGAGGTAGTCAATCTGTCATGACTTACTCCGGTGACGATCCAGCGCGAGGC
GATGAGAAGGAGCGGGGCAG

Downstream 100 bases:

>100_bases
GTTAGGTTCCAAGCCGGTCGGGACCTACCGCATCGGCACCAAAGGCGCTGATGCGAGCGGGAAAAATCCGCGCAACACCC
AGGTCGGTGGATACGTAGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREHDAILLGAVGDPSVPSGVLER
GLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVVREGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQ
DAFERARQRRKHLTLVHKNNVLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL
AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGEDEPAARLDQAVASYLATRGN
GRFSTGEVGERIAAAL

Sequences:

>Translated_336_residues
MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREHDAILLGAVGDPSVPSGVLER
GLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVVREGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQ
DAFERARQRRKHLTLVHKNNVLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL
AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGEDEPAARLDQAVASYLATRGN
GRFSTGEVGERIAAAL
>Mature_336_residues
MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREHDAILLGAVGDPSVPSGVLER
GLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVVREGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQ
DAFERARQRRKHLTLVHKNNVLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL
AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGEDEPAARLDQAVASYLATRGN
GRFSTGEVGERIAAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=328, Percent_Identity=33.5365853658537, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4758582, Length=329, Percent_Identity=27.6595744680851, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI28178821, Length=299, Percent_Identity=28.7625418060201, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178816, Length=300, Percent_Identity=28.6666666666667, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=27.0516717325228, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI28178819, Length=146, Percent_Identity=34.2465753424658, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788101, Length=328, Percent_Identity=42.9878048780488, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=38.2436260623229, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787381, Length=354, Percent_Identity=28.8135593220339, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71986051, Length=329, Percent_Identity=34.6504559270517, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17550882, Length=334, Percent_Identity=31.437125748503, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=336, Percent_Identity=29.4642857142857, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=27.5449101796407, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=332, Percent_Identity=35.2409638554217, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=319, Percent_Identity=31.974921630094, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319830, Length=359, Percent_Identity=32.8690807799443, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=332, Percent_Identity=31.9277108433735, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=331, Percent_Identity=32.6283987915408, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24643268, Length=331, Percent_Identity=32.6283987915408, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24661184, Length=316, Percent_Identity=32.9113924050633, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281362242, Length=328, Percent_Identity=27.7439024390244, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648872, Length=328, Percent_Identity=27.7439024390244, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078635, Length=311, Percent_Identity=27.6527331189711, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078633, Length=311, Percent_Identity=27.6527331189711, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650122, Length=311, Percent_Identity=27.6527331189711, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078637, Length=311, Percent_Identity=27.6527331189711, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078639, Length=306, Percent_Identity=28.4313725490196, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20130355, Length=319, Percent_Identity=22.5705329153605, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_MYCLB (B8ZS15)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503792.1
- ProteinModelPortal:   B8ZS15
- SMR:   B8ZS15
- EnsemblBacteria:   EBMYCT00000086703
- GeneID:   7326793
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016921
- HOGENOM:   HBG518924
- OMA:   AATIFMV
- ProtClustDB:   PRK03437
- GO:   GO:0005737
- HAMAP:   MF_01035
- InterPro:   IPR019818
- InterPro:   IPR001804
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 35792; Mature: 35792

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 87-87 BINDING 97-97 BINDING 121-121 BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREH
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEECCHHHHHHHHHHHHHHHC
DAILLGAVGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVV
CEEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCCCHHHCCCCHHHHHCCCCCCCEEEE
REGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNN
ECCCCCCCCCCCCEEEECCCCHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
VLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL
EEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHHHHHHH
AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGE
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DEPAARLDQAVASYLATRGNGRFSTGEVGERIAAAL
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLAIIGGDGIGPEVVAQAVKILDVVLPGVQKTTYDLGARRYHTTGELLPESVLAELREH
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEECCHHHHHHHHHHHHHHHC
DAILLGAVGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDIDFVVV
CEEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCCCHHHCCCCHHHHHCCCCCCCEEEE
REGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERARQRRKHLTLVHKNN
ECCCCCCCCCCCCEEEECCCCHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
VLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGDIITDL
EEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHHHHHHH
AAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLLAHLGE
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
DEPAARLDQAVASYLATRGNGRFSTGEVGERIAAAL
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA