Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 221230372

GI number: 221230372

Start: 2029460

End: 2030056

Strand: Reverse

Name: leuD

Synonym: MLBr_01684

Alternate gene names: 221230372

Gene position: 2030056-2029460 (Counterclockwise)

Preceding gene: 221230373

Following gene: 221230371

Centisome position: 62.12

GC content: 55.28

Gene sequence:

>597_bases
ATGCAAGCTTTCCGCGTCCATACCGGTATCGGCGTGCCGTTGCGGCGGTCCAATGTCGATACCGATCAGATCATTCCGGC
GGTCTTTTTGAAGCGTGTCACTCGAAACGGTTTCGAGGACGGCTTGTTCGCTACGTGGCGGGTCGATCCCTCATTCGTGC
TCAACCTCAGCCCCTTTGATCGGGGCTCAGTCCTGGTCGTCGGTCCTGATTTCGGGATCGGGTCGTCGCGTGAGCATGCG
GTGTGGGCGCTCATGGACTACGGATTCCGGGTGGTTATCTCGTCTCGATTTGGTGATATTTTTCACGGTAACGCGGGCAA
GGCGGGGTTGCTGGCAGCTCAAGTAGCCCAAGATGATGTCGAATTTCTGTGGAAACTCATCGAGCAGAAGCCGGGGTTGG
AAATCACTGTCAATCTTCAAGATCGAAATATCAATGCTGCAACGGTGGTGTTGCCGTTCAAGATTGATGACTACACTGCT
TGGCGATTGCTCGAAGGACTCGACGATATAGCTCTTAATCTGCGGAAACTTGACGAGATCGAGGCGTTTGAGTCGGCGCG
TCCTGCGTGGAAACCGCGCACCCTGCCCACCACCTGA

Upstream 100 bases:

>100_bases
CGTCGCCGCTGCCACCGCGGTTCGCGGCACTTTCTCTGCTCCGGCCGATTTGAACTAACTCGGCCGGCTTGAACTGAGAT
AGAGGAGTGGAGGATAAATT

Downstream 100 bases:

>100_bases
CGGGTCGCACTGGCAGGATTTTTCGCTTGCTCACCAACACAGCCAGGCCCATTTTCTGTTCCCGCCACCGGTCAAGGGCG
GCAACCTGATTGCGATAATT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MQAFRVHTGIGVPLRRSNVDTDQIIPAVFLKRVTRNGFEDGLFATWRVDPSFVLNLSPFDRGSVLVVGPDFGIGSSREHA
VWALMDYGFRVVISSRFGDIFHGNAGKAGLLAAQVAQDDVEFLWKLIEQKPGLEITVNLQDRNINAATVVLPFKIDDYTA
WRLLEGLDDIALNLRKLDEIEAFESARPAWKPRTLPTT

Sequences:

>Translated_198_residues
MQAFRVHTGIGVPLRRSNVDTDQIIPAVFLKRVTRNGFEDGLFATWRVDPSFVLNLSPFDRGSVLVVGPDFGIGSSREHA
VWALMDYGFRVVISSRFGDIFHGNAGKAGLLAAQVAQDDVEFLWKLIEQKPGLEITVNLQDRNINAATVVLPFKIDDYTA
WRLLEGLDDIALNLRKLDEIEAFESARPAWKPRTLPTT
>Mature_198_residues
MQAFRVHTGIGVPLRRSNVDTDQIIPAVFLKRVTRNGFEDGLFATWRVDPSFVLNLSPFDRGSVLVVGPDFGIGSSREHA
VWALMDYGFRVVISSRFGDIFHGNAGKAGLLAAQVAQDDVEFLWKLIEQKPGLEITVNLQDRNINAATVVLPFKIDDYTA
WRLLEGLDDIALNLRKLDEIEAFESARPAWKPRTLPTT

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=42.1319796954315, Blast_Score=161, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321429, Length=201, Percent_Identity=39.3034825870647, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_MYCLB (B8ZS11)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503788.1
- ProteinModelPortal:   B8ZS11
- SMR:   B8ZS11
- EnsemblBacteria:   EBMYCT00000086969
- GeneID:   7326789
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015493
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22137; Mature: 22137

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFRVHTGIGVPLRRSNVDTDQIIPAVFLKRVTRNGFEDGLFATWRVDPSFVLNLSPFD
CCEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCC
RGSVLVVGPDFGIGSSREHAVWALMDYGFRVVISSRFGDIFHGNAGKAGLLAAQVAQDDV
CCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHH
EFLWKLIEQKPGLEITVNLQDRNINAATVVLPFKIDDYTAWRLLEGLDDIALNLRKLDEI
HHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEEEECCHHHHHHHHCHHHHHHHHHHHHHH
EAFESARPAWKPRTLPTT
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQAFRVHTGIGVPLRRSNVDTDQIIPAVFLKRVTRNGFEDGLFATWRVDPSFVLNLSPFD
CCEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCC
RGSVLVVGPDFGIGSSREHAVWALMDYGFRVVISSRFGDIFHGNAGKAGLLAAQVAQDDV
CCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHH
EFLWKLIEQKPGLEITVNLQDRNINAATVVLPFKIDDYTAWRLLEGLDDIALNLRKLDEI
HHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEEEECCHHHHHHHHCHHHHHHHHHHHHHH
EAFESARPAWKPRTLPTT
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA