Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 221230373

GI number: 221230373

Start: 2030099

End: 2031556

Strand: Reverse

Name: leuC [H]

Synonym: MLBr_01685

Alternate gene names: 221230373

Gene position: 2031556-2030099 (Counterclockwise)

Preceding gene: 221230374

Following gene: 221230372

Centisome position: 62.16

GC content: 60.49

Gene sequence:

>1458_bases
TTGGAGATGGATAACGAGAGGAAACAAGTGGGTGGCGCCCGCAAACCGCGCACGTTGGCCGAAAAGGTTTGGGACGACCA
CGTTGTGGTAGCCGGTCAGGGCTACCATCAAGATCGGGGTCCCGATTTGATTTACATCGATCTACATTTGGTGCACGAGG
TCACCAGCCCGCAGGCTTTCGATGGCCTGCGCTTGGCCGGCCGGTGGGTGCGGCGCCCCGATTTAACAGTGGCCACCGAG
GATCACAACGTGCCCACCGTCGACATCGACAAGCCGATCGCTGACCCGGTGTCGCGCATTCAAGTGGAGACGTTGCGGCG
CAACTGCGCCGAATTTGGTGTCCGGTTACACCCGATGGGCGATATCGAGCAGGGCATCGTTCATGTCGTAGGGCCACAGT
TAGGCCTCACTCAACCGGGCATGACGATCGTTTGCGGCGACAGTCACACCTCTACCCATGGCGCTTTTGGTGCAATCGCG
ATGGGCATCGGCACCTCGGAGATCGAGCATGTACTCGCTACCCAAACGCTGCCGTTGCGCCCATTCAAGACAATGGCGGT
CACTGTCGATGGGCGGTTGCCCGCCGGCGTCACAGCCAAGGACATCATCCTTGCGTTGATTGCTAAGATTGGCACCGGCG
GCGGGCAGGGCTATGTACTCGAATACCGGGGCAGTGTCATCGAATCGATGTCCATGGAAGGACGGATGACGATCTGCAAC
ATGAGCATCGAAGCCGGTGCTCGAGCAGGAATGGTGGCTCCCGACGAGACTACCTACGAGTTCTTACGTGACCGTCCGCA
CGCACCGACTGGCAAGCAATGGGATGCCGCAGTAGCCTACTGGCAACAGCTGCGCACTGACGACGACGCTGTGTTCGACA
CCGAGGTCTATCTCGATGCGACCTCGTTGAGCCCGTTTGTCACCTGGGGGACAAACCCCGGTCAAGGAGTGCCGTTGGCC
GCATCTGTGCCTGATCCCGAACTGATGATTGACGATGTTGCCCGACAAGCCGCCGAGAAAGCTTTGGCGTACATGGACCT
TCGCCCAGGTACACCGATGCGCGATATTGCGGTCGATGCGGTGTTCGTTGGGTCGTGTACCAACGGCCGCATTGAGGATC
TGCGGTTGGTGGCCGATGTGTTACGTGGCCACAAGGTGGCCGACGGTGTGCGGATGCTGATCGTCCCCGGCTCGATGCGG
GTACGTGCGCAGGCTGAAGCCGAGGGGCTCGGTGAGATTTTTATTACTGCGGGAGCGCAGTGGCGGCAGCCGGGTTGTTC
GATGTGTCTGGGCATGAACCCCGATCAGCTTGCGCCCGGGGAGCGGTGCGCTGCGACGTCCAACCGAAACTTCGAAGGAC
GGCAAGGCAAGGGTGGCCGTACACATTTGGTATCCCCGGCCGTCGCCGCTGCCACCGCGGTTCGCGGCACTTTCTCTGCT
CCGGCCGATTTGAACTAA

Upstream 100 bases:

>100_bases
CCGATACCGCTAGTCTGTGTCACAGAGAGATATCAGTCTCGTATTCTGCGATATCAATATGGATTGATCGGCGAGCCGCA
AATGAGGCGAATCGAGGCGC

Downstream 100 bases:

>100_bases
CTCGGCCGGCTTGAACTGAGATAGAGGAGTGGAGGATAAATTATGCAAGCTTTCCGCGTCCATACCGGTATCGGCGTGCC
GTTGCGGCGGTCCAATGTCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MEMDNERKQVGGARKPRTLAEKVWDDHVVVAGQGYHQDRGPDLIYIDLHLVHEVTSPQAFDGLRLAGRWVRRPDLTVATE
DHNVPTVDIDKPIADPVSRIQVETLRRNCAEFGVRLHPMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIA
MGIGTSEIEHVLATQTLPLRPFKTMAVTVDGRLPAGVTAKDIILALIAKIGTGGGQGYVLEYRGSVIESMSMEGRMTICN
MSIEAGARAGMVAPDETTYEFLRDRPHAPTGKQWDAAVAYWQQLRTDDDAVFDTEVYLDATSLSPFVTWGTNPGQGVPLA
ASVPDPELMIDDVARQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRLVADVLRGHKVADGVRMLIVPGSMR
VRAQAEAEGLGEIFITAGAQWRQPGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTFSA
PADLN

Sequences:

>Translated_485_residues
MEMDNERKQVGGARKPRTLAEKVWDDHVVVAGQGYHQDRGPDLIYIDLHLVHEVTSPQAFDGLRLAGRWVRRPDLTVATE
DHNVPTVDIDKPIADPVSRIQVETLRRNCAEFGVRLHPMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIA
MGIGTSEIEHVLATQTLPLRPFKTMAVTVDGRLPAGVTAKDIILALIAKIGTGGGQGYVLEYRGSVIESMSMEGRMTICN
MSIEAGARAGMVAPDETTYEFLRDRPHAPTGKQWDAAVAYWQQLRTDDDAVFDTEVYLDATSLSPFVTWGTNPGQGVPLA
ASVPDPELMIDDVARQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRLVADVLRGHKVADGVRMLIVPGSMR
VRAQAEAEGLGEIFITAGAQWRQPGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTFSA
PADLN
>Mature_485_residues
MEMDNERKQVGGARKPRTLAEKVWDDHVVVAGQGYHQDRGPDLIYIDLHLVHEVTSPQAFDGLRLAGRWVRRPDLTVATE
DHNVPTVDIDKPIADPVSRIQVETLRRNCAEFGVRLHPMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIA
MGIGTSEIEHVLATQTLPLRPFKTMAVTVDGRLPAGVTAKDIILALIAKIGTGGGQGYVLEYRGSVIESMSMEGRMTICN
MSIEAGARAGMVAPDETTYEFLRDRPHAPTGKQWDAAVAYWQQLRTDDDAVFDTEVYLDATSLSPFVTWGTNPGQGVPLA
ASVPDPELMIDDVARQAAEKALAYMDLRPGTPMRDIAVDAVFVGSCTNGRIEDLRLVADVLRGHKVADGVRMLIVPGSMR
VRAQAEAEGLGEIFITAGAQWRQPGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTFSA
PADLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=380, Percent_Identity=27.3684210526316, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI8659555, Length=451, Percent_Identity=24.8337028824834, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI41352693, Length=366, Percent_Identity=23.224043715847, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=61.3390928725702, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=24.585635359116, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=22.96918767507, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI2367097, Length=113, Percent_Identity=35.3982300884956, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=378, Percent_Identity=28.8359788359788, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=378, Percent_Identity=28.8359788359788, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=29.3375394321767, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=375, Percent_Identity=25.8666666666667, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=462, Percent_Identity=56.9264069264069, Blast_Score=542, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6322261, Length=403, Percent_Identity=28.0397022332506, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=491, Percent_Identity=26.4765784114053, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=27.9778393351801, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281365315, Length=499, Percent_Identity=25.250501002004, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17864292, Length=499, Percent_Identity=25.250501002004, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=25.6345177664975, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=397, Percent_Identity=25.9445843828715, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=24.8648648648649, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=25.4742547425474, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 52141; Mature: 52141

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMDNERKQVGGARKPRTLAEKVWDDHVVVAGQGYHQDRGPDLIYIDLHLVHEVTSPQAF
CCCCCHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCHH
DGLRLAGRWVRRPDLTVATEDHNVPTVDIDKPIADPVSRIQVETLRRNCAEFGVRLHPMG
CCHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCEEECCCC
DIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIAMGIGTSEIEHVLATQTLPLR
CHHHCCEEEECCCCCCCCCCCEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHCCCCCC
PFKTMAVTVDGRLPAGVTAKDIILALIAKIGTGGGQGYVLEYRGSVIESMSMEGRMTICN
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCCCCEEEEE
MSIEAGARAGMVAPDETTYEFLRDRPHAPTGKQWDAAVAYWQQLRTDDDAVFDTEVYLDA
EEHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEE
TSLSPFVTWGTNPGQGVPLAASVPDPELMIDDVARQAAEKALAYMDLRPGTPMRDIAVDA
CCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHEEEE
VFVGSCTNGRIEDLRLVADVLRGHKVADGVRMLIVPGSMRVRAQAEAEGLGEIFITAGAQ
EEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCEEEEECCCC
WRQPGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTFSA
CCCCCCCEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCC
PADLN
CCCCC
>Mature Secondary Structure
MEMDNERKQVGGARKPRTLAEKVWDDHVVVAGQGYHQDRGPDLIYIDLHLVHEVTSPQAF
CCCCCHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCHH
DGLRLAGRWVRRPDLTVATEDHNVPTVDIDKPIADPVSRIQVETLRRNCAEFGVRLHPMG
CCHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCEEECCCC
DIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIAMGIGTSEIEHVLATQTLPLR
CHHHCCEEEECCCCCCCCCCCEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHCCCCCC
PFKTMAVTVDGRLPAGVTAKDIILALIAKIGTGGGQGYVLEYRGSVIESMSMEGRMTICN
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCCCCEEEEE
MSIEAGARAGMVAPDETTYEFLRDRPHAPTGKQWDAAVAYWQQLRTDDDAVFDTEVYLDA
EEHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEE
TSLSPFVTWGTNPGQGVPLAASVPDPELMIDDVARQAAEKALAYMDLRPGTPMRDIAVDA
CCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHEEEE
VFVGSCTNGRIEDLRLVADVLRGHKVADGVRMLIVPGSMRVRAQAEAEGLGEIFITAGAQ
EEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCEEEEECCCC
WRQPGCSMCLGMNPDQLAPGERCAATSNRNFEGRQGKGGRTHLVSPAVAAATAVRGTFSA
CCCCCCCEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCC
PADLN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]