Click here to switch to the map view.

The map label for this gene is hup

Identifier: 221230371

GI number: 221230371

Start: 2028644

End: 2029246

Strand: Reverse

Name: hup

Synonym: MLBr_01683

Alternate gene names: 221230371

Gene position: 2029246-2028644 (Counterclockwise)

Preceding gene: 221230372

Following gene: 221230367

Centisome position: 62.09

GC content: 61.36

Gene sequence:

>603_bases
ATGAACAAAGCAGAGCTCATTGACGTGCTGACACAGAAATTGGGCTCGGACCGTCGGCAGGCGACCGCTGCCGTCGAGAA
TGTCGTTGACACCATTGTGCGTGCTGTACACAAGGGCGACAGCGTCACCATTACCGGGTTCGGTGTGTTCGAGCAGCGTC
GCCGGGCTGCGCGCGTGGCTCGTAACCCCCGTACCGGCGAAACGGTGAAGGTGAAGCCGACGTCCGTCCCGGCGTTTCGT
CCGGGTGCGCAATTTAAAGCGGTTGTGGCTGGCGCACAGCGTCTCCCGTTGGAAGGTCCCGCTGTCAAGCGTGGTGTAGC
GACCAGCGCTGCCAAGAAGGCAGCGATTAAGAAGGCTCCGGTTAAGAAGGCGCTGGCCAAGAAGGCGGCGACCAAGGCTC
CGGCCAAGAAGGCCGTGAAGGCGCCCGCCAAGAAAATCACCACGGCCGTGAAAGTTCCGGCTAAAAAGGCGACAAAAGTG
GTCAAGAAGGTCGCCGCCAAGGCTCCGGTACGCAAGGCCACGACCAGGGCGCTGGCCAAGAAGGCAGCGGTGAAGAAGGC
TCCTGCCAAGAAGGTTACCGCAGCTAAGCGGGGTCGCAAGTAA

Upstream 100 bases:

>100_bases
CGGTGAAATTGCGCGTGGCTCTTGGAAATCAATGGGCTGAGGGTTTACCGTGTCCATTAGTCGGTTCAAAAACGAGGACC
ACTGACTTCGGAGGATTTGG

Downstream 100 bases:

>100_bases
AGAAAATAGACAGTCTAACGCAGATACCTTTCGGTAGGCGTTACTTTCACCCGGAGGGTATCTGCGTGTTAGGGCTGTAC
GTTGGCTGCGAGCGTGCTGC

Product: putative histone-like protein

Products: NA

Alternate protein names: 21 kDa laminin-2-binding protein; Histone-like protein; Hlp

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVARNPRTGETVKVKPTSVPAFR
PGAQFKAVVAGAQRLPLEGPAVKRGVATSAAKKAAIKKAPVKKALAKKAATKAPAKKAVKAPAKKITTAVKVPAKKATKV
VKKVAAKAPVRKATTRALAKKAAVKKAPAKKVTAAKRGRK

Sequences:

>Translated_200_residues
MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVARNPRTGETVKVKPTSVPAFR
PGAQFKAVVAGAQRLPLEGPAVKRGVATSAAKKAAIKKAPVKKALAKKAATKAPAKKAVKAPAKKITTAVKVPAKKATKV
VKKVAAKAPVRKATTRALAKKAAVKKAPAKKVTAAKRGRK
>Mature_200_residues
MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVARNPRTGETVKVKPTSVPAFR
PGAQFKAVVAGAQRLPLEGPAVKRGVATSAAKKAAIKKAPVKKALAKKAATKAPAKKAVKAPAKKITTAVKVPAKKATKV
VKKVAAKAPVRKATTRALAKKAAVKKAPAKKVTAAKRGRK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Secreted, cell wall

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family

Homologues:

Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=41.5730337078652, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1788005, Length=92, Percent_Identity=39.1304347826087, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786644, Length=91, Percent_Identity=37.3626373626374, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): DBH_MYCLE (O33125)

Other databases:

- EMBL:   AB022517
- EMBL:   Z99263
- EMBL:   AL583923
- PIR:   T45427
- RefSeq:   NP_302157.1
- ProteinModelPortal:   O33125
- SMR:   O33125
- EnsemblBacteria:   EBMYCT00000028992
- GeneID:   910050
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1683
- NMPDR:   fig|272631.1.peg.1029
- Leproma:   ML1683
- GeneTree:   EBGT00050000014972
- HOGENOM:   HBG705085
- OMA:   HKGESVT
- ProtClustDB:   CLSK872125
- BioCyc:   MLEP272631:ML1683-MONOMER
- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992
- Gene3D:   G3DSA:4.10.520.10
- PRINTS:   PR01727
- SMART:   SM00411

Pfam domain/function: PF00216 Bac_DNA_binding; SSF47729 IHF_like_DNA_bnd

EC number: NA

Molecular weight: Translated: 20970; Mature: 20970

Theoretical pI: Translated: 12.38; Mature: 12.38

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVA
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
RNPRTGETVKVKPTSVPAFRPGAQFKAVVAGAQRLPLEGPAVKRGVATSAAKKAAIKKAP
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VKKALAKKAATKAPAKKAVKAPAKKITTAVKVPAKKATKVVKKVAAKAPVRKATTRALAK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
KAAVKKAPAKKVTAAKRGRK
HHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MNKAELIDVLTQKLGSDRRQATAAVENVVDTIVRAVHKGDSVTITGFGVFEQRRRAARVA
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
RNPRTGETVKVKPTSVPAFRPGAQFKAVVAGAQRLPLEGPAVKRGVATSAAKKAAIKKAP
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VKKALAKKAATKAPAKKAVKAPAKKITTAVKVPAKKATKVVKKVAAKAPVRKATTRALAK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
KAAVKKAPAKKVTAAKRGRK
HHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10449784; 11234002