Click here to switch to the map view.

The map label for this gene is thyA

Identifier: 221230278

GI number: 221230278

Start: 1832949

End: 1833749

Strand: Reverse

Name: thyA

Synonym: MLBr_01519

Alternate gene names: 221230278

Gene position: 1833749-1832949 (Counterclockwise)

Preceding gene: 221230279

Following gene: 221230277

Centisome position: 56.11

GC content: 56.3

Gene sequence:

>801_bases
GTGCTGATAGCGACGCCATACGAGGATTTGCTGCGTCTCGTGCTTGACTGCGGCGTAGCCAAAATGGACCGCACCGGCAC
CGGGACTCGCAGTCTGTTTGGTCAGCAGTTGCGTTACGACCTGTCGGTTGGGTTCCCGCTAGTTACGACGAAAAAGGTGC
ACTTCAAATCGGTTGTCTACGAGTTGTTGTGGTTCTTGCGCGGCGACTCCAACGTCGCCTGGTTGCATGAGCATGGAGTT
AATATCTGGGATGAGTGGGCTAGCAGCACAGGCGACCTCGGGCCGATTTATGGTGTGCAGTGGCGATCTTGGCCGACCTC
TTCGGGTGATTACATCGACCAAATCAGCACCGCACTGGATTTGCTGCGTACCGAACCGGATTCTCGGCGCATCATTGTGT
CGGCTTGGAATGTCGGTGAAATCCCGCAGATGGCGCTGCCACCATGTCACGCGTTTTTCCAATTCTACGTCGCTCAAGGC
CGGCTGAGTTGCCAGCTGTACCAGCGCAGCGCCGACATGTTCCTTGGTGTGCCGTTCAACATCGCCAGTTACGCGCTTCT
GACCCACATGATGGCCGCCCAGTCCGGTCTTTTGGTTGGTGAGTTCGTCTGGACGGGCGGTGATTGCCACATCTACGACA
ACCATGTCGAGCAGGTACAGCTGCAGCTCAGCCGCGAACCCCGAGCATATCCAGAACTCTTTTTAGCGCAGCGTGATTCT
ATCTTCGACTATGTCTACGAGGACGTCGTCGTCACGAATTATGATCCGCACCCGGCGATCAAAGCCCCAGTCGCGATATG
A

Upstream 100 bases:

>100_bases
TGTTCTACTCTTCGTGGCGCGGCGTAAATTCATTGGCTTGTTGGCGTCGATCCGCCGCGCCTGGCTTCGCTGTGCTTGCG
ACCGCCGCTAAACTGGCTGC

Downstream 100 bases:

>100_bases
CGCGCGCCGACGATGATGGTGCCAGCATGGCTGGGATCGAGGAGTGGTGCGTATGACGGTGGTGGGCCTGATCTGGGCTC
AGTCGACGACGGGTGTGATT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLIATPYEDLLRLVLDCGVAKMDRTGTGTRSLFGQQLRYDLSVGFPLVTTKKVHFKSVVYELLWFLRGDSNVAWLHEHGV
NIWDEWASSTGDLGPIYGVQWRSWPTSSGDYIDQISTALDLLRTEPDSRRIIVSAWNVGEIPQMALPPCHAFFQFYVAQG
RLSCQLYQRSADMFLGVPFNIASYALLTHMMAAQSGLLVGEFVWTGGDCHIYDNHVEQVQLQLSREPRAYPELFLAQRDS
IFDYVYEDVVVTNYDPHPAIKAPVAI

Sequences:

>Translated_266_residues
MLIATPYEDLLRLVLDCGVAKMDRTGTGTRSLFGQQLRYDLSVGFPLVTTKKVHFKSVVYELLWFLRGDSNVAWLHEHGV
NIWDEWASSTGDLGPIYGVQWRSWPTSSGDYIDQISTALDLLRTEPDSRRIIVSAWNVGEIPQMALPPCHAFFQFYVAQG
RLSCQLYQRSADMFLGVPFNIASYALLTHMMAAQSGLLVGEFVWTGGDCHIYDNHVEQVQLQLSREPRAYPELFLAQRDS
IFDYVYEDVVVTNYDPHPAIKAPVAI
>Mature_266_residues
MLIATPYEDLLRLVLDCGVAKMDRTGTGTRSLFGQQLRYDLSVGFPLVTTKKVHFKSVVYELLWFLRGDSNVAWLHEHGV
NIWDEWASSTGDLGPIYGVQWRSWPTSSGDYIDQISTALDLLRTEPDSRRIIVSAWNVGEIPQMALPPCHAFFQFYVAQG
RLSCQLYQRSADMFLGVPFNIASYALLTHMMAAQSGLLVGEFVWTGGDCHIYDNHVEQVQLQLSREPRAYPELFLAQRDS
IFDYVYEDVVVTNYDPHPAIKAPVAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=274, Percent_Identity=51.0948905109489, Blast_Score=278, Evalue=5e-75,
Organism=Escherichia coli, GI1789191, Length=261, Percent_Identity=66.2835249042146, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71993377, Length=275, Percent_Identity=45.8181818181818, Blast_Score=243, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6324648, Length=231, Percent_Identity=43.2900432900433, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=49.469964664311, Blast_Score=275, Evalue=2e-74,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_MYCLE (Q9CBW0)

Other databases:

- EMBL:   AL583922
- PIR:   A87099
- RefSeq:   NP_302064.1
- ProteinModelPortal:   Q9CBW0
- SMR:   Q9CBW0
- EnsemblBacteria:   EBMYCT00000028728
- GeneID:   909552
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1519
- NMPDR:   fig|272631.1.peg.936
- Leproma:   ML1519
- GeneTree:   EBGT00050000017116
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   MLEP272631:ML1519-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30142; Mature: 30142

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIATPYEDLLRLVLDCGVAKMDRTGTGTRSLFGQQLRYDLSVGFPLVTTKKVHFKSVVY
CEEECCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHH
ELLWFLRGDSNVAWLHEHGVNIWDEWASSTGDLGPIYGVQWRSWPTSSGDYIDQISTALD
HHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
LLRTEPDSRRIIVSAWNVGEIPQMALPPCHAFFQFYVAQGRLSCQLYQRSADMFLGVPFN
HHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEHEECCCCCEEEECCHH
IASYALLTHMMAAQSGLLVGEFVWTGGDCHIYDNHVEQVQLQLSREPRAYPELFLAQRDS
HHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHH
IFDYVYEDVVVTNYDPHPAIKAPVAI
HHHHHHHCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLIATPYEDLLRLVLDCGVAKMDRTGTGTRSLFGQQLRYDLSVGFPLVTTKKVHFKSVVY
CEEECCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHH
ELLWFLRGDSNVAWLHEHGVNIWDEWASSTGDLGPIYGVQWRSWPTSSGDYIDQISTALD
HHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
LLRTEPDSRRIIVSAWNVGEIPQMALPPCHAFFQFYVAQGRLSCQLYQRSADMFLGVPFN
HHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEHEECCCCCEEEECCHH
IASYALLTHMMAAQSGLLVGEFVWTGGDCHIYDNHVEQVQLQLSREPRAYPELFLAQRDS
HHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHH
IFDYVYEDVVVTNYDPHPAIKAPVAI
HHHHHHHCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002