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The map label for this gene is folA

Identifier: 221230277

GI number: 221230277

Start: 1832399

End: 1832896

Strand: Reverse

Name: folA

Synonym: MLBr_01518

Alternate gene names: 221230277

Gene position: 1832896-1832399 (Counterclockwise)

Preceding gene: 221230278

Following gene: 221230276

Centisome position: 56.08

GC content: 61.24

Gene sequence:

>498_bases
ATGACGGTGGTGGGCCTGATCTGGGCTCAGTCGACGACGGGTGTGATTGGGCGCGACGGCGGCATGCCATGGCGAGTGCC
TGAGGATCTGACTCGCTTCAAACAGCTGACGATGGGTCACCCGGTGGTGATGGGCCGACGGACCTGGGATTCGTTGCCGG
TTGGGGTTCGCCCGCTGCCAGGCCGCCGAAATATCGTGTTGAGCCGACAAATTGACTTTATGGCTGAGGGAGCGGAGGTG
TTCGGTTCTCTCGAAGAGACCATTTCCAATCTCGAGACCGAGCCTGAGATGTGGGTGATTGGCGGTGAGCAGATCTACCG
GCTTGCGTTGCCGCTGGCGACCCGTTGCGGGGTCACCGAGGTAGACACCTACCTGCTTCATGAGGACGATGACGTGCTGG
CCCCGGTGCTCGACGATACATGGGTGGGTGTGACGGGAGAGTGGCTGGTGAGCTGCTCCGGGCTGCGGTACCGGTTGCAC
AGCTATCATCGTTCATGA

Upstream 100 bases:

>100_bases
ACGAATTATGATCCGCACCCGGCGATCAAAGCCCCAGTCGCGATATGACGCGCGCCGACGATGATGGTGCCAGCATGGCT
GGGATCGAGGAGTGGTGCGT

Downstream 100 bases:

>100_bases
GCGCTGTTTTCCGCGGCAAGTAGGGTGGGTTATCCCCACATGTATCGTTGCGGCGCGTTTGCATGAGCACTGTTACTACC
GTACAGGCCTGTCGAGTAGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTVVGLIWAQSTTGVIGRDGGMPWRVPEDLTRFKQLTMGHPVVMGRRTWDSLPVGVRPLPGRRNIVLSRQIDFMAEGAEV
FGSLEETISNLETEPEMWVIGGEQIYRLALPLATRCGVTEVDTYLLHEDDDVLAPVLDDTWVGVTGEWLVSCSGLRYRLH
SYHRS

Sequences:

>Translated_165_residues
MTVVGLIWAQSTTGVIGRDGGMPWRVPEDLTRFKQLTMGHPVVMGRRTWDSLPVGVRPLPGRRNIVLSRQIDFMAEGAEV
FGSLEETISNLETEPEMWVIGGEQIYRLALPLATRCGVTEVDTYLLHEDDDVLAPVLDDTWVGVTGEWLVSCSGLRYRLH
SYHRS
>Mature_164_residues
TVVGLIWAQSTTGVIGRDGGMPWRVPEDLTRFKQLTMGHPVVMGRRTWDSLPVGVRPLPGRRNIVLSRQIDFMAEGAEVF
GSLEETISNLETEPEMWVIGGEQIYRLALPLATRCGVTEVDTYLLHEDDDVLAPVLDDTWVGVTGEWLVSCSGLRYRLHS
YHRS

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain

Homologues:

Organism=Escherichia coli, GI1786233, Length=146, Percent_Identity=37.6712328767123, Blast_Score=90, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17505899, Length=124, Percent_Identity=39.5161290322581, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=139, Percent_Identity=30.2158273381295, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI116008026, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24647458, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DYR_MYCLE (Q9CBW1)

Other databases:

- EMBL:   AL583922
- PIR:   H87098
- RefSeq:   NP_302063.1
- ProteinModelPortal:   Q9CBW1
- EnsemblBacteria:   EBMYCT00000029190
- GeneID:   909551
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1518
- NMPDR:   fig|272631.1.peg.935
- Leproma:   ML1518
- GeneTree:   EBGT00050000017411
- HOGENOM:   HBG665708
- OMA:   GLRYRFH
- ProtClustDB:   CLSK792062
- BioCyc:   MLEP272631:ML1518-MONOMER
- BRENDA:   1.5.1.3
- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796
- PANTHER:   PTHR11549:SF1
- PRINTS:   PR00070

Pfam domain/function: PF00186 DHFR_1

EC number: =1.5.1.3

Molecular weight: Translated: 18509; Mature: 18378

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00075 DHFR_1; PS51330 DHFR_2

Important sites: BINDING 29-29 BINDING 62-62 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVGLIWAQSTTGVIGRDGGMPWRVPEDLTRFKQLTMGHPVVMGRRTWDSLPVGVRPLP
CEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
GRRNIVLSRQIDFMAEGAEVFGSLEETISNLETEPEMWVIGGEQIYRLALPLATRCGVTE
CCCCEEEECCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCHHEEHHHHHHHHHCCCCC
VDTYLLHEDDDVLAPVLDDTWVGVTGEWLVSCSGLRYRLHSYHRS
CCEEEEECCCCEEECCCCCCCCCCCHHHEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
TVVGLIWAQSTTGVIGRDGGMPWRVPEDLTRFKQLTMGHPVVMGRRTWDSLPVGVRPLP
EEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
GRRNIVLSRQIDFMAEGAEVFGSLEETISNLETEPEMWVIGGEQIYRLALPLATRCGVTE
CCCCEEEECCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCHHEEHHHHHHHHHCCCCC
VDTYLLHEDDDVLAPVLDDTWVGVTGEWLVSCSGLRYRLHSYHRS
CCEEEEECCCCEEECCCCCCCCCCCHHHEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002