Click here to switch to the map view.

The map label for this gene is cobB

Identifier: 221230272

GI number: 221230272

Start: 1821166

End: 1821879

Strand: Direct

Name: cobB

Synonym: MLBr_01511

Alternate gene names: 221230272

Gene position: 1821166-1821879 (Clockwise)

Preceding gene: 221230270

Following gene: 221230282

Centisome position: 55.73

GC content: 61.76

Gene sequence:

>714_bases
ATGCGGGTGGTAGTGCTTAGCGGCGCGGGTATCTCCGCGGAAAGCGACGTGCCGACATTCCGCGACGACAAGAATGGATT
GTGGGCACGCTTCGATCCTTACCAGCTGTCCAGCACGCAAGGCTGGCAGCGCAACCCTGAGCGGGTCTGGGGGTGGTACT
TGTGGCGCCACTACCTGGTAGCCAACGTCAAACCCAACGATGGCCACCGCGCTATAGCCGCCTGGCAGGAGCAGATCGAG
GTTAGCGTCATCACCCAAAATGTTGACGATTTGCACGAGCGCGCCGGCAGCACGCCGGTGCACCATCTGCACGGCAGCCT
TTTCAAATTTCATTGTGCCCGCTGCAATGTGGCCTACACCGGTGCACTTCCCGATATGCCCGAACCCGTACTAGAGGTGG
ACCCACCGGTCTGCTACTGCGGCGGTCTGATCCGGCCTGCCATCGTGTGGTTCGGTGAGCCATTACCCGATGAGCCGTGG
CGACGCGCAGTGGAGGCGACCGAAACCACCGACGTCATGGTGGTGGTGGGGACATCCGCGATCGTCTACCCGGCGGCCGG
GCTACCCGAGCTGGCACTGTCACGTGGTGCGGTTGTGATCGAAGTTAATCCCGAGCCCACACCGCTCACCAAGAACGCCA
CGATCAGCATTCGTGAGACTGCAAGTCAGGCATTGCCAGGACTGCTGCAGCGGCTTCCCGCCCTGTTGAAGTAG

Upstream 100 bases:

>100_bases
GGTTTTTGAGCTGGTCGAACAACGGTTTGCCCGCCTTTACGTCGATCCCGCCGCTCCACCCACCCATCCAGTATCTCCCA
AGCCCGGCTATCTTTATAGC

Downstream 100 bases:

>100_bases
GCGTAGCGCTCTCTCGGGGTTGAATTGTACTTTGTGTGCATGTTCTGTTGTGGGGGTGGAGTAAAGGGTGGCGGTGTGTT
GCAGTGTTGTGAGTTGATGT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRVVVLSGAGISAESDVPTFRDDKNGLWARFDPYQLSSTQGWQRNPERVWGWYLWRHYLVANVKPNDGHRAIAAWQEQIE
VSVITQNVDDLHERAGSTPVHHLHGSLFKFHCARCNVAYTGALPDMPEPVLEVDPPVCYCGGLIRPAIVWFGEPLPDEPW
RRAVEATETTDVMVVVGTSAIVYPAAGLPELALSRGAVVIEVNPEPTPLTKNATISIRETASQALPGLLQRLPALLK

Sequences:

>Translated_237_residues
MRVVVLSGAGISAESDVPTFRDDKNGLWARFDPYQLSSTQGWQRNPERVWGWYLWRHYLVANVKPNDGHRAIAAWQEQIE
VSVITQNVDDLHERAGSTPVHHLHGSLFKFHCARCNVAYTGALPDMPEPVLEVDPPVCYCGGLIRPAIVWFGEPLPDEPW
RRAVEATETTDVMVVVGTSAIVYPAAGLPELALSRGAVVIEVNPEPTPLTKNATISIRETASQALPGLLQRLPALLK
>Mature_237_residues
MRVVVLSGAGISAESDVPTFRDDKNGLWARFDPYQLSSTQGWQRNPERVWGWYLWRHYLVANVKPNDGHRAIAAWQEQIE
VSVITQNVDDLHERAGSTPVHHLHGSLFKFHCARCNVAYTGALPDMPEPVLEVDPPVCYCGGLIRPAIVWFGEPLPDEPW
RRAVEATETTDVMVVVGTSAIVYPAAGLPELALSRGAVVIEVNPEPTPLTKNATISIRETASQALPGLLQRLPALLK

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=232, Percent_Identity=37.5, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI6912664, Length=252, Percent_Identity=37.3015873015873, Blast_Score=150, Evalue=6e-37,
Organism=Homo sapiens, GI13787215, Length=233, Percent_Identity=39.0557939914163, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI6912662, Length=256, Percent_Identity=26.953125, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI6912660, Length=200, Percent_Identity=32, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI13775602, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI13775600, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI63054862, Length=196, Percent_Identity=30.6122448979592, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI300797705, Length=188, Percent_Identity=28.1914893617021, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI7657575, Length=226, Percent_Identity=25.6637168141593, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=25.1968503937008, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI308199517, Length=245, Percent_Identity=37.1428571428571, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17541892, Length=216, Percent_Identity=31.0185185185185, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990482, Length=263, Percent_Identity=28.1368821292776, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567771, Length=264, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990487, Length=262, Percent_Identity=28.2442748091603, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325242, Length=217, Percent_Identity=31.7972350230415, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571445, Length=186, Percent_Identity=32.7956989247312, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648389, Length=197, Percent_Identity=27.4111675126904, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_MYCLE (Q9CBW6)

Other databases:

- EMBL:   AL583922
- PIR:   A87098
- RefSeq:   NP_302058.1
- ProteinModelPortal:   Q9CBW6
- SMR:   Q9CBW6
- EnsemblBacteria:   EBMYCT00000029237
- GeneID:   909544
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1511
- NMPDR:   fig|272631.1.peg.930
- Leproma:   ML1511
- GeneTree:   EBGT00050000016307
- HOGENOM:   HBG641281
- OMA:   DADGLWE
- ProtClustDB:   PRK00481
- BioCyc:   MLEP272631:ML1511-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26159; Mature: 26159

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVVLSGAGISAESDVPTFRDDKNGLWARFDPYQLSSTQGWQRNPERVWGWYLWRHYLV
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHEE
ANVKPNDGHRAIAAWQEQIEVSVITQNVDDLHERAGSTPVHHLHGSLFKFHCARCNVAYT
EECCCCCCCHHHHHHHHHEEEEEEECCHHHHHHHHCCCCHHHHHHHHHEEHHHHCCEEEE
GALPDMPEPVLEVDPPVCYCGGLIRPAIVWFGEPLPDEPWRRAVEATETTDVMVVVGTSA
CCCCCCCCCCEECCCCCEECCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCE
IVYPAAGLPELALSRGAVVIEVNPEPTPLTKNATISIRETASQALPGLLQRLPALLK
EEECCCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVVVLSGAGISAESDVPTFRDDKNGLWARFDPYQLSSTQGWQRNPERVWGWYLWRHYLV
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHEE
ANVKPNDGHRAIAAWQEQIEVSVITQNVDDLHERAGSTPVHHLHGSLFKFHCARCNVAYT
EECCCCCCCHHHHHHHHHEEEEEEECCHHHHHHHHCCCCHHHHHHHHHEEHHHHCCEEEE
GALPDMPEPVLEVDPPVCYCGGLIRPAIVWFGEPLPDEPWRRAVEATETTDVMVVVGTSA
CCCCCCCCCCEECCCCCEECCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCE
IVYPAAGLPELALSRGAVVIEVNPEPTPLTKNATISIRETASQALPGLLQRLPALLK
EEECCCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002