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The map label for this gene is ald [H]

Identifier: 221230282

GI number: 221230282

Start: 1846890

End: 1848005

Strand: Direct

Name: ald [H]

Synonym: MLBr_01532

Alternate gene names: 221230282

Gene position: 1846890-1848005 (Clockwise)

Preceding gene: 221230272

Following gene: 221230293

Centisome position: 56.51

GC content: 60.84

Gene sequence:

>1116_bases
ATGCGAGTCGGCATTCCGACCGAGACCAAAAACAACGAATTCAGGGTGGCCATCACGCCGGCCGGCGTGGCCGCCCTGAC
ACACCGCGGCCACGAGGTGCTTGTGCAAGCAGGCGCCGGAGAAGGCTCGGCAATCGCCGACGCGGAGTTCAAGGCAGCAG
GAGCCCAACTGATCAGCACTCCCGAGCAGGTGTGGACCGAGGTCGACCTGCTACTCAAGGTCAAAGAGCCCATGCCCGCA
GAGTATGTTCACCTACGACACGGCCAGATCCTGTTTACGTATCTGCATCTAGCTGCCTCACGCCCTTGCACCGACGCATT
ATTGGCCTCCGGCACAACGTCAATCGCCTACGAGACCGTGCAGACCGCCGACGGCGCGCTGCCGTTACTAGCTCCAATGA
GCGAGGTTGCCGGACGACTGTCGGCTCAGGTAGGGGCCTATCACCTAATGCGGACCCACAGGGGGTGCGGGGTACTGATG
GGTGGAGTGCCCGGCGTCGAACCCGCTGACGTGGTCGTGATCGGCGGCGGGACTGCGGGATATAACGCCGCTCGAATTGC
CAACGGTATGGGTGCGACCGTCACGGTTTTTGATGTCAACATCAACAGACTTCGGGAGCTCGACGCGGAGTTCGGTGGCC
GCCTCCGCACCCGCTACTCGTCGGCCTACGACCTAGAAGGTGCGGTCAAGCACGCCGACCTGGTGATCGGCGCCGTCCTG
GTGCCAGGCGCCAAAGCCCCCCGATTGATATCGAATTCGCTTGTCGCACATATGAAGCCGGGTGCTGTGTTGGTCGACAT
CGCCATCGATCAGGGCGGTTGTTTCGAAGATTCGCAGCCGACCACCCATGATCAACCGACGTTCGCGGTGCATGACACGC
TGTTCTACTGTGTGGCAAACATGCCTAGCGCGGTGCCCAAGACATCGACATTCGCGCTGACCAATGCAACGATGCCATAT
ATACTCAAACTCGCGGACCAAGGCTGGCAGGAAGCCTGCCAATCGGATCCCGCTTTGGCAAAAGGTCTTTCGACGCATAA
CGGTGCACTGTTGTCTGAACAGGTAGCCACCGACCTGGGATTGGCCTTCACCGAACCGGCAAGCGTGCTGGCCTGA

Upstream 100 bases:

>100_bases
ATCAGCGATATCGATATAGCATCAGAGGGATTGCGGACTATCCGAACTTTCTTCACACTTGAGCCATAACCTCAACGATC
TTGAGCCAGGGGGAGTGATT

Downstream 100 bases:

>100_bases
CGTCAATCCCGCCCGGCCAGTAACCAGTAACTTGTCCGTTTAGACTTCAAGGTGTCGTCGTGATGGTCATTTGGTGGTTA
TTTTTTGCTGGTTTGGGTTT

Product: L-alanine dehydrogenase

Products: NA

Alternate protein names: 40 kDa antigen; TB43 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRVGIPTETKNNEFRVAITPAGVAALTHRGHEVLVQAGAGEGSAIADAEFKAAGAQLISTPEQVWTEVDLLLKVKEPMPA
EYVHLRHGQILFTYLHLAASRPCTDALLASGTTSIAYETVQTADGALPLLAPMSEVAGRLSAQVGAYHLMRTHRGCGVLM
GGVPGVEPADVVVIGGGTAGYNAARIANGMGATVTVFDVNINRLRELDAEFGGRLRTRYSSAYDLEGAVKHADLVIGAVL
VPGAKAPRLISNSLVAHMKPGAVLVDIAIDQGGCFEDSQPTTHDQPTFAVHDTLFYCVANMPSAVPKTSTFALTNATMPY
ILKLADQGWQEACQSDPALAKGLSTHNGALLSEQVATDLGLAFTEPASVLA

Sequences:

>Translated_371_residues
MRVGIPTETKNNEFRVAITPAGVAALTHRGHEVLVQAGAGEGSAIADAEFKAAGAQLISTPEQVWTEVDLLLKVKEPMPA
EYVHLRHGQILFTYLHLAASRPCTDALLASGTTSIAYETVQTADGALPLLAPMSEVAGRLSAQVGAYHLMRTHRGCGVLM
GGVPGVEPADVVVIGGGTAGYNAARIANGMGATVTVFDVNINRLRELDAEFGGRLRTRYSSAYDLEGAVKHADLVIGAVL
VPGAKAPRLISNSLVAHMKPGAVLVDIAIDQGGCFEDSQPTTHDQPTFAVHDTLFYCVANMPSAVPKTSTFALTNATMPY
ILKLADQGWQEACQSDPALAKGLSTHNGALLSEQVATDLGLAFTEPASVLA
>Mature_371_residues
MRVGIPTETKNNEFRVAITPAGVAALTHRGHEVLVQAGAGEGSAIADAEFKAAGAQLISTPEQVWTEVDLLLKVKEPMPA
EYVHLRHGQILFTYLHLAASRPCTDALLASGTTSIAYETVQTADGALPLLAPMSEVAGRLSAQVGAYHLMRTHRGCGVLM
GGVPGVEPADVVVIGGGTAGYNAARIANGMGATVTVFDVNINRLRELDAEFGGRLRTRYSSAYDLEGAVKHADLVIGAVL
VPGAKAPRLISNSLVAHMKPGAVLVDIAIDQGGCFEDSQPTTHDQPTFAVHDTLFYCVANMPSAVPKTSTFALTNATMPY
ILKLADQGWQEACQSDPALAKGLSTHNGALLSEQVATDLGLAFTEPASVLA

Specific function: May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=308, Percent_Identity=32.4675324675325, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI122939153, Length=308, Percent_Identity=32.4675324675325, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1787887, Length=302, Percent_Identity=32.4503311258278, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17550456, Length=346, Percent_Identity=29.7687861271676, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38846; Mature: 38846

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGIPTETKNNEFRVAITPAGVAALTHRGHEVLVQAGAGEGSAIADAEFKAAGAQLIST
CCCCCCCCCCCCEEEEEECCCCHHHHHCCCCEEEEEECCCCCCEEECCHHHHCCHHHHCC
PEQVWTEVDLLLKVKEPMPAEYVHLRHGQILFTYLHLAASRPCTDALLASGTTSIAYETV
HHHHHHHHHHEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCHHEEEHH
QTADGALPLLAPMSEVAGRLSAQVGAYHLMRTHRGCGVLMGGVPGVEPADVVVIGGGTAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCC
YNAARIANGMGATVTVFDVNINRLRELDAEFGGRLRTRYSSAYDLEGAVKHADLVIGAVL
CCHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHEEEEEE
VPGAKAPRLISNSLVAHMKPGAVLVDIAIDQGGCFEDSQPTTHDQPTFAVHDTLFYCVAN
CCCCCCCHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHC
MPSAVPKTSTFALTNATMPYILKLADQGWQEACQSDPALAKGLSTHNGALLSEQVATDLG
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHC
LAFTEPASVLA
CEECCCHHHCC
>Mature Secondary Structure
MRVGIPTETKNNEFRVAITPAGVAALTHRGHEVLVQAGAGEGSAIADAEFKAAGAQLIST
CCCCCCCCCCCCEEEEEECCCCHHHHHCCCCEEEEEECCCCCCEEECCHHHHCCHHHHCC
PEQVWTEVDLLLKVKEPMPAEYVHLRHGQILFTYLHLAASRPCTDALLASGTTSIAYETV
HHHHHHHHHHEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCHHEEEHH
QTADGALPLLAPMSEVAGRLSAQVGAYHLMRTHRGCGVLMGGVPGVEPADVVVIGGGTAG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCC
YNAARIANGMGATVTVFDVNINRLRELDAEFGGRLRTRYSSAYDLEGAVKHADLVIGAVL
CCHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHEEEEEE
VPGAKAPRLISNSLVAHMKPGAVLVDIAIDQGGCFEDSQPTTHDQPTFAVHDTLFYCVAN
CCCCCCCHHHHHHHHEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHC
MPSAVPKTSTFALTNATMPYILKLADQGWQEACQSDPALAKGLSTHNGALLSEQVATDLG
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHC
LAFTEPASVLA
CEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587598; 9634230; 12218036; 7822223 [H]