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The map label for this gene is pimE [H]

Identifier: 221230268

GI number: 221230268

Start: 1815291

End: 1816583

Strand: Reverse

Name: pimE [H]

Synonym: MLBr_01504

Alternate gene names: 221230268

Gene position: 1816583-1815291 (Counterclockwise)

Preceding gene: 221230271

Following gene: 221230266

Centisome position: 55.59

GC content: 61.87

Gene sequence:

>1293_bases
GTGGTGGCAGTGATCATGGCAAAGGCGTTTGCCGCGGTCGCTAGAGTCGGGCAGATAGACACCACCACCACTAACACCAC
AGCCGCCGCGTCGGCACCCCGCGTCCGACGGCTGACGAGCCGGTTGAATGCTGCCGCGCCGTTACTGTTGGTCTTCAGCG
TCGCGGTGCGACTGGCTTGGACCTACCTGGCACCCAACGGAGCAAACTTCGTTGACCTGCACGTTTACCTGGGTGGCGCG
GCTACGATCGACCATCCCGGCACCTTGTACCGTTACGTGTACGTCGACCAGACACCGGACTTCCCGCTCCCGTTTACCTA
TCCGCCGTTTGCAGCAGTGGTCTTCTACCCGCTGCATTTGTTGCCGTTTGCTCTGGTGGCGTTTCTGTGGCAAATCACCA
CGATCGCTGCATTGTATGGCGCGGTTCGGATCAGCCAGCGCTTACTGGGTGTTTCCTCCCATACCGGTCACCGCGTTGCG
CTGTTGTGGACGGCGATCGCCATCTGGATCGAGCCTCTGCGCAGCGCCTTCGACTACGGGCAGATCAATGTGCTGCCAAT
GCTGGGGGTGCTGTGGGCGGTCTACACCACGCGTAACTGGCTATCGGGGTTGCTGGTCGGTGTGGCGGGCGGAATCAAGT
TAACGCCGGCGATCGCAGCTGTCTATTTCCTCGGGGCCCGACGCGTTCGCGCAGCAGCGTTGTCGGCGGCCGTGTTTGGT
GCCACCGTCGCGGTGTCGGCTCTCGTCGTTGGTGATCAGGCTCGTTACTACTTCACTGAGCTGCTGGGCGACGCGCATCG
AGTTGGTCCTATCGCCACCTCGTTCAATCAATCGTGGCGCGGCGGCATCTCGCGGATCATGGGCTACGACGCTGGGTTGG
GCCCGTTGGTCCTCGCTGCGATCGCCATCACCGCTGTAGTGGCGGTTGTTGCTTGGCGAGCGCTCGATCGGCCCGACCGA
CTGGGCAAATTGCTGGTGGTCGAGTTGTTCGGACTGCTGCTTTCGCCGATTTCCTGGACTCATCACTGGGTGTGGCTGGT
GCCTCTGATGATCTGGCTGATTCACGGGCCGCTGGGCGAGCGCCCCGGTGCCCAGATTGTGGGTTGGGGCTGGTTGGCGC
TGAGTGTCGTCGGCGTGCCGTGGTTGCTCAGTTTTGCTCAACCGACCATCTGGCAATTAGGCAGACCGTGGTATCTGGCG
TGGGGCGGCCTGGTCTATATCGTGGTGGCGCTGGTGACGTTGATCTGGATTGTCGTCATCGGGAAACACCCTGTAGTGGC
TAGTCCCCGATGA

Upstream 100 bases:

>100_bases
TCTCCCTGGTGATTGACGACAGGTCCGGACCCGAAACTACTTTGATACGTATGTGACTCAAGTGACATATGTGGTATTTA
TTCGATCGTTACCCAGATGA

Downstream 100 bases:

>100_bases
GCCTGTCGATATCGCGTGCCATCGTGATGTCCTTCTTGGTGATGCCATCGGCATAATGTGTCACTAGAATGAACGTAACT
GTCCGATAACGGATATCGAT

Product: mannosyltransferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Alpha-manT; PPM-dependent mannosyltransferase; Polyprenol-phosphate-mannose alpha-mannosyltransferase; PPM alpha-mannosyltransferase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MVAVIMAKAFAAVARVGQIDTTTTNTTAAASAPRVRRLTSRLNAAAPLLLVFSVAVRLAWTYLAPNGANFVDLHVYLGGA
ATIDHPGTLYRYVYVDQTPDFPLPFTYPPFAAVVFYPLHLLPFALVAFLWQITTIAALYGAVRISQRLLGVSSHTGHRVA
LLWTAIAIWIEPLRSAFDYGQINVLPMLGVLWAVYTTRNWLSGLLVGVAGGIKLTPAIAAVYFLGARRVRAAALSAAVFG
ATVAVSALVVGDQARYYFTELLGDAHRVGPIATSFNQSWRGGISRIMGYDAGLGPLVLAAIAITAVVAVVAWRALDRPDR
LGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIHGPLGERPGAQIVGWGWLALSVVGVPWLLSFAQPTIWQLGRPWYLA
WGGLVYIVVALVTLIWIVVIGKHPVVASPR

Sequences:

>Translated_430_residues
MVAVIMAKAFAAVARVGQIDTTTTNTTAAASAPRVRRLTSRLNAAAPLLLVFSVAVRLAWTYLAPNGANFVDLHVYLGGA
ATIDHPGTLYRYVYVDQTPDFPLPFTYPPFAAVVFYPLHLLPFALVAFLWQITTIAALYGAVRISQRLLGVSSHTGHRVA
LLWTAIAIWIEPLRSAFDYGQINVLPMLGVLWAVYTTRNWLSGLLVGVAGGIKLTPAIAAVYFLGARRVRAAALSAAVFG
ATVAVSALVVGDQARYYFTELLGDAHRVGPIATSFNQSWRGGISRIMGYDAGLGPLVLAAIAITAVVAVVAWRALDRPDR
LGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIHGPLGERPGAQIVGWGWLALSVVGVPWLLSFAQPTIWQLGRPWYLA
WGGLVYIVVALVTLIWIVVIGKHPVVASPR
>Mature_430_residues
MVAVIMAKAFAAVARVGQIDTTTTNTTAAASAPRVRRLTSRLNAAAPLLLVFSVAVRLAWTYLAPNGANFVDLHVYLGGA
ATIDHPGTLYRYVYVDQTPDFPLPFTYPPFAAVVFYPLHLLPFALVAFLWQITTIAALYGAVRISQRLLGVSSHTGHRVA
LLWTAIAIWIEPLRSAFDYGQINVLPMLGVLWAVYTTRNWLSGLLVGVAGGIKLTPAIAAVYFLGARRVRAAALSAAVFG
ATVAVSALVVGDQARYYFTELLGDAHRVGPIATSFNQSWRGGISRIMGYDAGLGPLVLAAIAITAVVAVVAWRALDRPDR
LGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIHGPLGERPGAQIVGWGWLALSVVGVPWLLSFAQPTIWQLGRPWYLA
WGGLVYIVVALVTLIWIVVIGKHPVVASPR

Specific function: Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PIGM family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018584 [H]

Pfam domain/function: PF09594 DUF2029 [H]

EC number: NA

Molecular weight: Translated: 46601; Mature: 46601

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVIMAKAFAAVARVGQIDTTTTNTTAAASAPRVRRLTSRLNAAAPLLLVFSVAVRLAW
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYLAPNGANFVDLHVYLGGAATIDHPGTLYRYVYVDQTPDFPLPFTYPPFAAVVFYPLHL
HHCCCCCCCEEEEEEEECCEEEECCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LPFALVAFLWQITTIAALYGAVRISQRLLGVSSHTGHRVALLWTAIAIWIEPLRSAFDYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
QINVLPMLGVLWAVYTTRNWLSGLLVGVAGGIKLTPAIAAVYFLGARRVRAAALSAAVFG
CEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVAVSALVVGDQARYYFTELLGDAHRVGPIATSFNQSWRGGISRIMGYDAGLGPLVLAA
HHHHHHHHHCCCCHHHHHHHHHCCHHHCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
IAITAVVAVVAWRALDRPDRLGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIHGPLGE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
RPGAQIVGWGWLALSVVGVPWLLSFAQPTIWQLGRPWYLAWGGLVYIVVALVTLIWIVVI
CCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
GKHPVVASPR
CCCCCCCCCC
>Mature Secondary Structure
MVAVIMAKAFAAVARVGQIDTTTTNTTAAASAPRVRRLTSRLNAAAPLLLVFSVAVRLAW
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYLAPNGANFVDLHVYLGGAATIDHPGTLYRYVYVDQTPDFPLPFTYPPFAAVVFYPLHL
HHCCCCCCCEEEEEEEECCEEEECCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
LPFALVAFLWQITTIAALYGAVRISQRLLGVSSHTGHRVALLWTAIAIWIEPLRSAFDYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
QINVLPMLGVLWAVYTTRNWLSGLLVGVAGGIKLTPAIAAVYFLGARRVRAAALSAAVFG
CEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATVAVSALVVGDQARYYFTELLGDAHRVGPIATSFNQSWRGGISRIMGYDAGLGPLVLAA
HHHHHHHHHCCCCHHHHHHHHHCCHHHCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
IAITAVVAVVAWRALDRPDRLGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIHGPLGE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
RPGAQIVGWGWLALSVVGVPWLLSFAQPTIWQLGRPWYLAWGGLVYIVVALVTLIWIVVI
CCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
GKHPVVASPR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]