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The map label for this gene is fadD21

Identifier: 221230109

GI number: 221230109

Start: 1467600

End: 1469339

Strand: Reverse

Name: fadD21

Synonym: MLBr_01234

Alternate gene names: 221230109

Gene position: 1469339-1467600 (Counterclockwise)

Preceding gene: 221230117

Following gene: 221230108

Centisome position: 44.96

GC content: 57.93

Gene sequence:

>1740_bases
ATGCAAAACTCGTCCGTCCTTTCTTTCCTGCGCGAACGTGCCGGCCTGCAGCCAGACGATGAAGCATTCAGCTTCACCGA
CTACGAGCAGGATTGGGCGGGTGTCCGCAAAACACTGACTTGGGCGCAGCTGTATCAACGAACCTTGAACGTTGCGCACG
AGCTCAGGCGGCACGGTTCGATAAGAGACCGGGCAGTGATATTGGCTCCCCAGGGGCTGGACTATATCATCGCGTTCCTG
GGAGCTATGCAGGCTGGCTTTATCGCCGTTCCGCTGTCAGTACCTCAGGCCGGCTCCCATGACGAACGTGTCGGAGCTGT
GCTTGCCGACACATCACCCTCGGTCGTCCTGACAACGTCTGCGGTTGCCGATGCGATCGCGAAATACGTTGACCACTCTG
ACACCGACACCGTCCCGGCGATCCTCGAAGTCGACTCACCAAATCTAGACGTCGAGAATAAATCGAATATTAGACTCACC
GATGCGCCGAGCACAGCGTACTTACAGTACACCTCGGGTTCGACCCGCCTTCCAGCCGGGGTCATGGTCACGCACCGGAA
CCTTATGGTGAATTTCCAACAGTTGATGGCTGACTACTTCGCTCCCACTAACGGTGTGGCGCCACTCGATCTCACCATCG
TGTCGTGGCTGCCGTTCTACCACGATATGGGCTTGGTTCTCGGCGTCGTCGCACCAATTCTGGGCGGCTGGCGGAGCGAG
CTTACGAGTCCGATCTCGTTTTTGCAACGTCCAGCCAGGTGGATCCAAGCGATGGCCACCAGCAGCCACCCATTTTCTGC
AGGACCGAACTTCGCCTTCGAATTGGCTGCACGCCGGACATCAGACGCAGATATAGCCGGCCTCGATCTCGGCGCTTGCC
AGGGCATCATCAGCGGCAGTGAACGAATCCACCCCGCCACGCTAAATCGGTTCTCGGATCGGTTTGCCCGAATCAATTTC
CGCGACGACATGATGCTACCCTCCTACGGGTTAGCTGAAGGAACTGTCTATGCCGCCAGCCGCCCTAAGGGAAGCTCGCC
AGAGGTCGTTTATTTCGAACCCGCGAAGCTGTCGGAAGGCACCGTGAAACGGTGCGAAGCACGGACCGGCGCGCCACTGC
TGAGCTACGGCACGCCGAAGTCACCAATAGTGCGAATCGTGGATTCCGACACGTGCATCGAGTGCCCAACAGGAAGGGTA
GGCGAAATCTGGCTACATGGTGACAACGTCGCCGAAGGCTATTGGCACAAACCGGAGGAAACGCAACGTACCTTCGGCGG
AAAGCTAGCTAACCCCTCGCCAGGCACACCCGAGGGACCGTGGTTGAGAACCGGAGACCTCGGGTTTATCTCCGAGGATG
AGCTGTTCATCGTGGGCCGTATGAAAGATCTGCTGATCGTGTATGGACGCAACCACTACCCTGAAGACATCGAATCGACG
GTCCAGGAAATCACCGGTGGTCGGGTCGCGGCGATATCCGTCCCGGTTGATGAGACCGAAAAATTGGTCACCATTATCGA
AGTCAAAAAGCGCGGCGACTCCGACGCGGAAGCGATGCAGAAGCTCGTCGCCGTCAAAAACAACATCACCGCGGCTATAT
CCAAGTCACATGGTCTGAACGTAGCCGACCTCGTTCTGGTACCGCCGGGGTCAATTCCTACCACGACCAGCGGCAAGGTT
AGGCGGACCGCCTGTGTCGAGCAGTATAGGCGGCAGCAGTTCTCCCGGTTAGACGGCTAA

Upstream 100 bases:

>100_bases
ATTCTGGTGAGTCGCGATTCGGTGCACAACCGTCTTTCCGTCTCATCCGCCCCACACCAGGCGTCCAGCCGATCCCAACT
GAGGCGGTAACCTAAAGCCC

Downstream 100 bases:

>100_bases
ATCACACTAGCTGGCAACCACGAATTGCCATGTGGATCACAATCCTATTGATGGCCATCGCAATAAGCCTCGAGCCATTC
CGGATCGGCATGACGGTACT

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MQNSSVLSFLRERAGLQPDDEAFSFTDYEQDWAGVRKTLTWAQLYQRTLNVAHELRRHGSIRDRAVILAPQGLDYIIAFL
GAMQAGFIAVPLSVPQAGSHDERVGAVLADTSPSVVLTTSAVADAIAKYVDHSDTDTVPAILEVDSPNLDVENKSNIRLT
DAPSTAYLQYTSGSTRLPAGVMVTHRNLMVNFQQLMADYFAPTNGVAPLDLTIVSWLPFYHDMGLVLGVVAPILGGWRSE
LTSPISFLQRPARWIQAMATSSHPFSAGPNFAFELAARRTSDADIAGLDLGACQGIISGSERIHPATLNRFSDRFARINF
RDDMMLPSYGLAEGTVYAASRPKGSSPEVVYFEPAKLSEGTVKRCEARTGAPLLSYGTPKSPIVRIVDSDTCIECPTGRV
GEIWLHGDNVAEGYWHKPEETQRTFGGKLANPSPGTPEGPWLRTGDLGFISEDELFIVGRMKDLLIVYGRNHYPEDIEST
VQEITGGRVAAISVPVDETEKLVTIIEVKKRGDSDAEAMQKLVAVKNNITAAISKSHGLNVADLVLVPPGSIPTTTSGKV
RRTACVEQYRRQQFSRLDG

Sequences:

>Translated_579_residues
MQNSSVLSFLRERAGLQPDDEAFSFTDYEQDWAGVRKTLTWAQLYQRTLNVAHELRRHGSIRDRAVILAPQGLDYIIAFL
GAMQAGFIAVPLSVPQAGSHDERVGAVLADTSPSVVLTTSAVADAIAKYVDHSDTDTVPAILEVDSPNLDVENKSNIRLT
DAPSTAYLQYTSGSTRLPAGVMVTHRNLMVNFQQLMADYFAPTNGVAPLDLTIVSWLPFYHDMGLVLGVVAPILGGWRSE
LTSPISFLQRPARWIQAMATSSHPFSAGPNFAFELAARRTSDADIAGLDLGACQGIISGSERIHPATLNRFSDRFARINF
RDDMMLPSYGLAEGTVYAASRPKGSSPEVVYFEPAKLSEGTVKRCEARTGAPLLSYGTPKSPIVRIVDSDTCIECPTGRV
GEIWLHGDNVAEGYWHKPEETQRTFGGKLANPSPGTPEGPWLRTGDLGFISEDELFIVGRMKDLLIVYGRNHYPEDIEST
VQEITGGRVAAISVPVDETEKLVTIIEVKKRGDSDAEAMQKLVAVKNNITAAISKSHGLNVADLVLVPPGSIPTTTSGKV
RRTACVEQYRRQQFSRLDG
>Mature_579_residues
MQNSSVLSFLRERAGLQPDDEAFSFTDYEQDWAGVRKTLTWAQLYQRTLNVAHELRRHGSIRDRAVILAPQGLDYIIAFL
GAMQAGFIAVPLSVPQAGSHDERVGAVLADTSPSVVLTTSAVADAIAKYVDHSDTDTVPAILEVDSPNLDVENKSNIRLT
DAPSTAYLQYTSGSTRLPAGVMVTHRNLMVNFQQLMADYFAPTNGVAPLDLTIVSWLPFYHDMGLVLGVVAPILGGWRSE
LTSPISFLQRPARWIQAMATSSHPFSAGPNFAFELAARRTSDADIAGLDLGACQGIISGSERIHPATLNRFSDRFARINF
RDDMMLPSYGLAEGTVYAASRPKGSSPEVVYFEPAKLSEGTVKRCEARTGAPLLSYGTPKSPIVRIVDSDTCIECPTGRV
GEIWLHGDNVAEGYWHKPEETQRTFGGKLANPSPGTPEGPWLRTGDLGFISEDELFIVGRMKDLLIVYGRNHYPEDIEST
VQEITGGRVAAISVPVDETEKLVTIIEVKKRGDSDAEAMQKLVAVKNNITAAISKSHGLNVADLVLVPPGSIPTTTSGKV
RRTACVEQYRRQQFSRLDG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI44888818, Length=555, Percent_Identity=25.045045045045, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI225735629, Length=552, Percent_Identity=24.2753623188406, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI45827692, Length=552, Percent_Identity=24.2753623188406, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI225735625, Length=612, Percent_Identity=23.8562091503268, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI55749758, Length=557, Percent_Identity=23.6983842010772, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI45827694, Length=559, Percent_Identity=24.1502683363148, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI187761345, Length=564, Percent_Identity=22.8723404255319, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI187761343, Length=564, Percent_Identity=22.8723404255319, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788107, Length=589, Percent_Identity=24.9575551782683, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI193202679, Length=603, Percent_Identity=24.3781094527363, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=494, Percent_Identity=23.8866396761134, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=541, Percent_Identity=21.81146025878, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654656, Length=587, Percent_Identity=24.3611584327087, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FAD21_MYCLE (P54200)

Other databases:

- EMBL:   U00010
- EMBL:   AL583921
- PIR:   D87063
- PIR:   S72711
- RefSeq:   NP_301895.1
- ProteinModelPortal:   P54200
- EnsemblBacteria:   EBMYCT00000028019
- GeneID:   910341
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1234
- NMPDR:   fig|272631.1.peg.767
- Leproma:   ML1234
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   SPYKTLT
- ProtClustDB:   PRK05850
- BioCyc:   MLEP272631:ML1234-MONOMER
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63103; Mature: 63103

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSSVLSFLRERAGLQPDDEAFSFTDYEQDWAGVRKTLTWAQLYQRTLNVAHELRRHGS
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IRDRAVILAPQGLDYIIAFLGAMQAGFIAVPLSVPQAGSHDERVGAVLADTSPSVVLTTS
CCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCEEEEECCCCCEEEEHH
AVADAIAKYVDHSDTDTVPAILEVDSPNLDVENKSNIRLTDAPSTAYLQYTSGSTRLPAG
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCC
VMVTHRNLMVNFQQLMADYFAPTNGVAPLDLTIVSWLPFYHDMGLVLGVVAPILGGWRSE
EEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LTSPISFLQRPARWIQAMATSSHPFSAGPNFAFELAARRTSDADIAGLDLGACQGIISGS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCC
ERIHPATLNRFSDRFARINFRDDMMLPSYGLAEGTVYAASRPKGSSPEVVYFEPAKLSEG
CCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCC
TVKRCEARTGAPLLSYGTPKSPIVRIVDSDTCIECPTGRVGEIWLHGDNVAEGYWHKPEE
HHHHHHHCCCCCEEECCCCCCCEEEEECCCCEEECCCCCEEEEEEECCCCCCCCCCCCHH
TQRTFGGKLANPSPGTPEGPWLRTGDLGFISEDELFIVGRMKDLLIVYGRNHYPEDIEST
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHH
VQEITGGRVAAISVPVDETEKLVTIIEVKKRGDSDAEAMQKLVAVKNNITAAISKSHGLN
HHHHCCCEEEEEECCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
VADLVLVPPGSIPTTTSGKVRRTACVEQYRRQQFSRLDG
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNSSVLSFLRERAGLQPDDEAFSFTDYEQDWAGVRKTLTWAQLYQRTLNVAHELRRHGS
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IRDRAVILAPQGLDYIIAFLGAMQAGFIAVPLSVPQAGSHDERVGAVLADTSPSVVLTTS
CCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCEEEEECCCCCEEEEHH
AVADAIAKYVDHSDTDTVPAILEVDSPNLDVENKSNIRLTDAPSTAYLQYTSGSTRLPAG
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCC
VMVTHRNLMVNFQQLMADYFAPTNGVAPLDLTIVSWLPFYHDMGLVLGVVAPILGGWRSE
EEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LTSPISFLQRPARWIQAMATSSHPFSAGPNFAFELAARRTSDADIAGLDLGACQGIISGS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCC
ERIHPATLNRFSDRFARINFRDDMMLPSYGLAEGTVYAASRPKGSSPEVVYFEPAKLSEG
CCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCC
TVKRCEARTGAPLLSYGTPKSPIVRIVDSDTCIECPTGRVGEIWLHGDNVAEGYWHKPEE
HHHHHHHCCCCCEEECCCCCCCEEEEECCCCEEECCCCCEEEEEEECCCCCCCCCCCCHH
TQRTFGGKLANPSPGTPEGPWLRTGDLGFISEDELFIVGRMKDLLIVYGRNHYPEDIEST
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHH
VQEITGGRVAAISVPVDETEKLVTIIEVKKRGDSDAEAMQKLVAVKNNITAAISKSHGLN
HHHHCCCEEEEEECCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
VADLVLVPPGSIPTTTSGKVRRTACVEQYRRQQFSRLDG
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002