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The map label for this gene is ilvE

Identifier: 221229868

GI number: 221229868

Start: 1030224

End: 1031330

Strand: Direct

Name: ilvE

Synonym: MLBr_00866

Alternate gene names: 221229868

Gene position: 1030224-1031330 (Clockwise)

Preceding gene: 221229867

Following gene: 221229871

Centisome position: 31.52

GC content: 61.52

Gene sequence:

>1107_bases
ATGACCAGCGGCTTCCTCGAGTTCACGGTGTCACTCTCGGCCCATCCGGTGACTGATGCGGAACGTGAATCGATCCTGGC
TGAGCCGGGCTTCGGTAAGTACCACACTGACCACATGGTGTCGATCGATTACATTGATGGCCGAGGTTGGCACGATGCGC
GGGTCATTCCCTACGGCCCGATTCAGCTGGATCCGTCCGCGATAGTGCTGCACTACGCGCAGGAAGTTTTTGAGGGACTC
AAGGCTTACCGCTGGGCGGACGGGTCGATCGTGTCTTTTCGCCCTGATGCTAACGCGGCCAGGTTGCGTTCGTCGGCGCG
GAGAATCGCGATTCCCGAGCTGCCCGACGAGTTATTCATTGACTCTCTGTGTCAGCTCATCGCTGTCGACAATGCTTGGG
TGCCCCGGGTCGGCGGTGAAGAGGCGCTTTATCTGCGGCCCTTCATCTTCGCGACCGAGCCGGGGCTGGGGGTACGGCCA
GCCAAGCAGTATCGGTACCTGTTGATCGCCTCGCCGGTCGGGGCGTATTTCAAAGGCGGCATCAACCCTGTCACGGTCTG
GGTGTCGATGGAGTACGTGCGAGCTAGCCCGGGCGGCACCGGTGCGGCCAAATTTGGCGGCAACTACGCTGCGTCGCTGC
TGGCTCAGACCGAAGCCGCAGCCAACGGGTGCGACCAGGTGGTGTGGCTAGACGCCGTCGAGCGCCGCTTCGTCGAAGAA
ATGGGCGGGATGAACATCTTCTTTGTGCTCGGCAGCGGCGGGTCGGCACGCCTGGTCACCCCGGAGCTGTCTGGCTCACT
GCTGCCCGGGGTCACACGCGCTTCGTTGCTGCAGTTGGCTATTGATGCCGGATTCAGTGTCGAAGAACGTAAGATCGATA
TCGACGAGTGGCAGAAGAAAGCTGCTGCTGGCGAGATCACCGAGGTGTTTGCCTGCGGCACTGCCGCCTTCATTACCCCG
GTCTCGCGGGTGAAATACGGTGATACCGAGTTTACCATCGCCGGTGGCGCACCAGGTGAGGTGACTATGGCGTTGCGCGA
CACGCTAACTGGAATCCAGCGCGGTACTTTTGCCGACACCCACGGCTGGATGGCCCGGCTGGGATAG

Upstream 100 bases:

>100_bases
GCGAAGTGGTGCGTCCGCCCTTCATCGAAGTGAAGACCCGCTAGCCTCTTTGCTTTAGCGCCACAAAAACCCGTTCGCAC
GCACATATACAATTGGCGCC

Downstream 100 bases:

>100_bases
TTGGTTGGGTGCCAGCTAAAACCGGACACCAAGACGACGAGTGGCATAGCGCTTAACGTTGTGGTGAGCTCGATCGCGAC
GCCCAGTATGTTACCGGTGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTSGFLEFTVSLSAHPVTDAERESILAEPGFGKYHTDHMVSIDYIDGRGWHDARVIPYGPIQLDPSAIVLHYAQEVFEGL
KAYRWADGSIVSFRPDANAARLRSSARRIAIPELPDELFIDSLCQLIAVDNAWVPRVGGEEALYLRPFIFATEPGLGVRP
AKQYRYLLIASPVGAYFKGGINPVTVWVSMEYVRASPGGTGAAKFGGNYAASLLAQTEAAANGCDQVVWLDAVERRFVEE
MGGMNIFFVLGSGGSARLVTPELSGSLLPGVTRASLLQLAIDAGFSVEERKIDIDEWQKKAAAGEITEVFACGTAAFITP
VSRVKYGDTEFTIAGGAPGEVTMALRDTLTGIQRGTFADTHGWMARLG

Sequences:

>Translated_368_residues
MTSGFLEFTVSLSAHPVTDAERESILAEPGFGKYHTDHMVSIDYIDGRGWHDARVIPYGPIQLDPSAIVLHYAQEVFEGL
KAYRWADGSIVSFRPDANAARLRSSARRIAIPELPDELFIDSLCQLIAVDNAWVPRVGGEEALYLRPFIFATEPGLGVRP
AKQYRYLLIASPVGAYFKGGINPVTVWVSMEYVRASPGGTGAAKFGGNYAASLLAQTEAAANGCDQVVWLDAVERRFVEE
MGGMNIFFVLGSGGSARLVTPELSGSLLPGVTRASLLQLAIDAGFSVEERKIDIDEWQKKAAAGEITEVFACGTAAFITP
VSRVKYGDTEFTIAGGAPGEVTMALRDTLTGIQRGTFADTHGWMARLG
>Mature_367_residues
TSGFLEFTVSLSAHPVTDAERESILAEPGFGKYHTDHMVSIDYIDGRGWHDARVIPYGPIQLDPSAIVLHYAQEVFEGLK
AYRWADGSIVSFRPDANAARLRSSARRIAIPELPDELFIDSLCQLIAVDNAWVPRVGGEEALYLRPFIFATEPGLGVRPA
KQYRYLLIASPVGAYFKGGINPVTVWVSMEYVRASPGGTGAAKFGGNYAASLLAQTEAAANGCDQVVWLDAVERRFVEEM
GGMNIFFVLGSGGSARLVTPELSGSLLPGVTRASLLQLAIDAGFSVEERKIDIDEWQKKAAAGEITEVFACGTAAFITPV
SRVKYGDTEFTIAGGAPGEVTMALRDTLTGIQRGTFADTHGWMARLG

Specific function: Acts on leucine, isoleucine and valine

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI296010904, Length=329, Percent_Identity=39.5136778115502, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI38176287, Length=329, Percent_Identity=39.5136778115502, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI296010906, Length=329, Percent_Identity=39.5136778115502, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI50658084, Length=338, Percent_Identity=38.7573964497041, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=38.7755102040816, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI296010902, Length=329, Percent_Identity=34.9544072948328, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI296010900, Length=329, Percent_Identity=34.9544072948328, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI48994963, Length=337, Percent_Identity=30.5637982195846, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568601, Length=344, Percent_Identity=38.953488372093, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17565728, Length=345, Percent_Identity=35.9420289855072, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322002, Length=334, Percent_Identity=39.5209580838323, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322608, Length=346, Percent_Identity=37.5722543352601, Blast_Score=220, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24641779, Length=341, Percent_Identity=39.8826979472141, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ILVE_MYCLE (O32954)

Other databases:

- EMBL:   Z98741
- EMBL:   AL583920
- PIR:   T44887
- RefSeq:   NP_301654.1
- ProteinModelPortal:   O32954
- SMR:   O32954
- EnsemblBacteria:   EBMYCT00000029369
- GeneID:   909864
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0866
- NMPDR:   fig|272631.1.peg.526
- Leproma:   ML0866
- GeneTree:   EBGT00050000017720
- HOGENOM:   HBG617123
- OMA:   YLRPFMF
- ProtClustDB:   PRK13357
- BioCyc:   MLEP272631:ML0866-MONOMER
- BRENDA:   2.6.1.42
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786
- PANTHER:   PTHR11825
- PANTHER:   PTHR11825:SF2
- PIRSF:   PIRSF006468
- TIGRFAMs:   TIGR01123

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.42

Molecular weight: Translated: 39716; Mature: 39584

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGFLEFTVSLSAHPVTDAERESILAEPGFGKYHTDHMVSIDYIDGRGWHDARVIPYGP
CCCCEEEEEEEECCCCCCCCHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCC
IQLDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRPDANAARLRSSARRIAIPELPDELFI
CEECCHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCEEECCCCCHHHHH
DSLCQLIAVDNAWVPRVGGEEALYLRPFIFATEPGLGVRPAKQYRYLLIASPVGAYFKGG
HHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHCC
INPVTVWVSMEYVRASPGGTGAAKFGGNYAASLLAQTEAAANGCDQVVWLDAVERRFVEE
CCEEEEEEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
MGGMNIFFVLGSGGSARLVTPELSGSLLPGVTRASLLQLAIDAGFSVEERKIDIDEWQKK
HCCCEEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHH
AAAGEITEVFACGTAAFITPVSRVKYGDTEFTIAGGAPGEVTMALRDTLTGIQRGTFADT
HHCCCHHHHHHCCCHHHHCHHHHEEECCCEEEEECCCCCEEEHHHHHHHHHHHCCCCCCC
HGWMARLG
CCHHHHCC
>Mature Secondary Structure 
TSGFLEFTVSLSAHPVTDAERESILAEPGFGKYHTDHMVSIDYIDGRGWHDARVIPYGP
CCCEEEEEEEECCCCCCCCHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCC
IQLDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRPDANAARLRSSARRIAIPELPDELFI
CEECCHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCEEECCCCCHHHHH
DSLCQLIAVDNAWVPRVGGEEALYLRPFIFATEPGLGVRPAKQYRYLLIASPVGAYFKGG
HHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHCC
INPVTVWVSMEYVRASPGGTGAAKFGGNYAASLLAQTEAAANGCDQVVWLDAVERRFVEE
CCEEEEEEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
MGGMNIFFVLGSGGSARLVTPELSGSLLPGVTRASLLQLAIDAGFSVEERKIDIDEWQKK
HCCCEEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHH
AAAGEITEVFACGTAAFITPVSRVKYGDTEFTIAGGAPGEVTMALRDTLTGIQRGTFADT
HHCCCHHHHHHCCCHHHHCHHHHEEECCCEEEEECCCCCEEEHHHHHHHHHHHCCCCCCC
HGWMARLG
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002