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The map label for this gene is gcvT

Identifier: 221229867

GI number: 221229867

Start: 1029064

End: 1030167

Strand: Direct

Name: gcvT

Synonym: MLBr_00865

Alternate gene names: 221229867

Gene position: 1029064-1030167 (Clockwise)

Preceding gene: 221229865

Following gene: 221229868

Centisome position: 31.49

GC content: 62.86

Gene sequence:

>1104_bases
GTGACCGATGCGCCAGAGCTTCTCAAGGGGCCGTTGGAAGACCGTCATCGCGAGCTTGGCGCGAATTTTGCCGAGTTCGG
TGGCTGGCTGATGCCGGTGTCGTATGCTGGCACCGTCAGTGAACACAGCGCCACCCGTAATGCTGTGGGTCTATTTGACG
TCAGCCACCTTGGTAAGGCGTTAGTTCGTGGGCCGGGTGCCGCGCAGTTCGTCAACTCCGTGCTCACCAACGACCTGGGC
CGCATACGGCCAGGTAAGGCGCAATATACGTTGTGCTGCTCAGAGTCTGGTGGTGTTATCGATGACCTGATTGCCTACTA
CGTCGATGACGATGAGATCTTTTTGGTGTCCAATGCCGCCAATACCGCCGCGGTGGTCGACGCATTGCAGGCGGTAGTGC
CGGCCGGCCTTACCATCATCAACCAGCATCGCTCCCACGCGGTGTTGGCGGTCCAGGGTCCTCGATCAACGGACGTGCTC
GGCGAGCTCGGGCTGCCAACTGGGATTGATTACATGGGTTACGTCGATGCTTCCTATGCGGGAGTACCGGTGCGCGTCTG
CCGCACCGGGTACACCGGCGAGCAAGGCTACGAACTGCTGCCGCCCTGGGAGTCAGCAGATGTGGTGTTCGACGCGTTGG
TCGCCGCTGTGGTTGACGCACGCGGGGAGCCGGCCGGCCTGGGCGCTCGTGACACACTGCGCACCGAGATGGGTTACCCG
CTGTATGGGCACGAACTTTCGCTTGACATCTCGCCGCTGCAGGCCCGGTGTGGCTGGGCGATTGGCTGGAAGAAGGATGC
GTTCTTGGGCCGGGATGCGTTGCTGGCCGAGAAGGCGGCGGGGCCGCGACGGCTGTTGCGGGGCCTGCGAATGGCTGGCC
GGGGAGTTTTGCGACCTGGGCTGACGGTGTGTGCTGGCGACATCCCGATCGGGGTCACCACGTCGGGGACGTTCTCGCCG
ACGTTGCAAGTTGGTGTCGCGTTGGCGTTGATCGACAGCGAAGCCGCGGTGCAGGACGGTCAGCAAATTATCGTCGACGT
TCGTGGCCGTGCTGTCGAGTGCGAAGTGGTGCGTCCGCCCTTCATCGAAGTGAAGACCCGCTAG

Upstream 100 bases:

>100_bases
TGGGCTGCCGTCGGCCGTGAAGTTTCATCCAGGTAGCTGATCAGCGCGCGGGAATCTCCCCACGTGAGCCAGTAGCGGAG
GTGGATTACGGTGGGACGAC

Downstream 100 bases:

>100_bases
CCTCTTTGCTTTAGCGCCACAAAAACCCGTTCGCACGCACATATACAATTGGCGCCATGACCAGCGGCTTCCTCGAGTTC
ACGGTGTCACTCTCGGCCCA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTDAPELLKGPLEDRHRELGANFAEFGGWLMPVSYAGTVSEHSATRNAVGLFDVSHLGKALVRGPGAAQFVNSVLTNDLG
RIRPGKAQYTLCCSESGGVIDDLIAYYVDDDEIFLVSNAANTAAVVDALQAVVPAGLTIINQHRSHAVLAVQGPRSTDVL
GELGLPTGIDYMGYVDASYAGVPVRVCRTGYTGEQGYELLPPWESADVVFDALVAAVVDARGEPAGLGARDTLRTEMGYP
LYGHELSLDISPLQARCGWAIGWKKDAFLGRDALLAEKAAGPRRLLRGLRMAGRGVLRPGLTVCAGDIPIGVTTSGTFSP
TLQVGVALALIDSEAAVQDGQQIIVDVRGRAVECEVVRPPFIEVKTR

Sequences:

>Translated_367_residues
MTDAPELLKGPLEDRHRELGANFAEFGGWLMPVSYAGTVSEHSATRNAVGLFDVSHLGKALVRGPGAAQFVNSVLTNDLG
RIRPGKAQYTLCCSESGGVIDDLIAYYVDDDEIFLVSNAANTAAVVDALQAVVPAGLTIINQHRSHAVLAVQGPRSTDVL
GELGLPTGIDYMGYVDASYAGVPVRVCRTGYTGEQGYELLPPWESADVVFDALVAAVVDARGEPAGLGARDTLRTEMGYP
LYGHELSLDISPLQARCGWAIGWKKDAFLGRDALLAEKAAGPRRLLRGLRMAGRGVLRPGLTVCAGDIPIGVTTSGTFSP
TLQVGVALALIDSEAAVQDGQQIIVDVRGRAVECEVVRPPFIEVKTR
>Mature_366_residues
TDAPELLKGPLEDRHRELGANFAEFGGWLMPVSYAGTVSEHSATRNAVGLFDVSHLGKALVRGPGAAQFVNSVLTNDLGR
IRPGKAQYTLCCSESGGVIDDLIAYYVDDDEIFLVSNAANTAAVVDALQAVVPAGLTIINQHRSHAVLAVQGPRSTDVLG
ELGLPTGIDYMGYVDASYAGVPVRVCRTGYTGEQGYELLPPWESADVVFDALVAAVVDARGEPAGLGARDTLRTEMGYPL
YGHELSLDISPLQARCGWAIGWKKDAFLGRDALLAEKAAGPRRLLRGLRMAGRGVLRPGLTVCAGDIPIGVTTSGTFSPT
LQVGVALALIDSEAAVQDGQQIIVDVRGRAVECEVVRPPFIEVKTR

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=368, Percent_Identity=28.5326086956522, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI257796258, Length=353, Percent_Identity=28.328611898017, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI257796254, Length=364, Percent_Identity=25.8241758241758, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI257796256, Length=312, Percent_Identity=26.9230769230769, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI24797151, Length=344, Percent_Identity=24.7093023255814, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI197927446, Length=284, Percent_Identity=28.5211267605634, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21361378, Length=284, Percent_Identity=28.5211267605634, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789272, Length=365, Percent_Identity=38.3561643835616, Blast_Score=210, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17560118, Length=373, Percent_Identity=30.2949061662198, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71994045, Length=396, Percent_Identity=23.2323232323232, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994052, Length=396, Percent_Identity=23.2323232323232, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32563613, Length=397, Percent_Identity=20.9068010075567, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320222, Length=382, Percent_Identity=30.8900523560209, Blast_Score=149, Evalue=5e-37,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=31.2997347480106, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI28571104, Length=292, Percent_Identity=24.3150684931507, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130091, Length=357, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_MYCLB (B8ZQK6)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503283.1
- ProteinModelPortal:   B8ZQK6
- SMR:   B8ZQK6
- EnsemblBacteria:   EBMYCT00000085108
- GeneID:   7327372
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015575
- HOGENOM:   HBG299834
- OMA:   NDISKPP
- ProtClustDB:   PRK00389
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 38880; Mature: 38749

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAPELLKGPLEDRHRELGANFAEFGGWLMPVSYAGTVSEHSATRNAVGLFDVSHLGKA
CCCCCHHHHCCHHHHHHHHCCCHHHHCCEEEEEEECCCCHHHHCCCCCEEEEEHHHHHHH
LVRGPGAAQFVNSVLTNDLGRIRPGKAQYTLCCSESGGVIDDLIAYYVDDDEIFLVSNAA
HHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHEECCCCEEEEECCC
NTAAVVDALQAVVPAGLTIINQHRSHAVLAVQGPRSTDVLGELGLPTGIDYMGYVDASYA
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCEECCCCC
GVPVRVCRTGYTGEQGYELLPPWESADVVFDALVAAVVDARGEPAGLGARDTLRTEMGYP
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC
LYGHELSLDISPLQARCGWAIGWKKDAFLGRDALLAEKAAGPRRLLRGLRMAGRGVLRPG
EECCEEEEECCCHHHHCCEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
LTVCAGDIPIGVTTSGTFSPTLQVGVALALIDSEAAVQDGQQIIVDVRGRAVECEVVRPP
CEEEECCCCEEEECCCCCCCHHHHEEEEEEECCHHHHHCCCEEEEEECCCEEEEEEECCC
FIEVKTR
CEEEECC
>Mature Secondary Structure 
TDAPELLKGPLEDRHRELGANFAEFGGWLMPVSYAGTVSEHSATRNAVGLFDVSHLGKA
CCCCHHHHCCHHHHHHHHCCCHHHHCCEEEEEEECCCCHHHHCCCCCEEEEEHHHHHHH
LVRGPGAAQFVNSVLTNDLGRIRPGKAQYTLCCSESGGVIDDLIAYYVDDDEIFLVSNAA
HHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHEECCCCEEEEECCC
NTAAVVDALQAVVPAGLTIINQHRSHAVLAVQGPRSTDVLGELGLPTGIDYMGYVDASYA
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCEECCCCC
GVPVRVCRTGYTGEQGYELLPPWESADVVFDALVAAVVDARGEPAGLGARDTLRTEMGYP
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC
LYGHELSLDISPLQARCGWAIGWKKDAFLGRDALLAEKAAGPRRLLRGLRMAGRGVLRPG
EECCEEEEECCCHHHHCCEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
LTVCAGDIPIGVTTSGTFSPTLQVGVALALIDSEAAVQDGQQIIVDVRGRAVECEVVRPP
CEEEECCCCEEEECCCCCCCHHHHEEEEEEECCHHHHHCCCEEEEEECCCEEEEEEECCC
FIEVKTR
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA