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The map label for this gene is pepA

Identifier: 221229866

GI number: 221229866

Start: 1027329

End: 1028903

Strand: Reverse

Name: pepA

Synonym: MLBr_00864

Alternate gene names: 221229866

Gene position: 1028903-1027329 (Counterclockwise)

Preceding gene: 221229869

Following gene: 221229863

Centisome position: 31.48

GC content: 61.65

Gene sequence:

>1575_bases
TTGCCCGTGACCACCGATCCGGGCTACCAAGGCCCTTCAGTCCATGTTGCCGCGTCGCTGCCAAAACACGATGTCAGTTC
TTCGGTATTGATCGTGCCCGTCGTCTCAGCCAGCGACAGCGACGAGTCCGAAGACCGGCCGGGCGCGGTCGTTGCTGCGG
CCGAACCTTTCCTAACCGCAGCTGCGGTCGCCGAAATCGAGGCCGGCCTGCGCGCACTGGAAGCCACAGGTGGCAGTGAT
CAGGTACACCGGTTAGTAGCACCGTCGCTACCGGTAGGCAGCGTGTTAACGGTCAGCTTGGGTAAGCCGCGGTCAGAGTG
GCCCGCCGATACGATCCGTCGCGCCGCTGGTGTGGCGGCGCGTTCACTCGGCAGAGCGGAAGTGGTTATCACCACACTGG
CCGAGTTGCCTGGGGAATCCGGTGCCAGTATCTGCTCAGCCGTCGTTGAGGGGCTGATGCTAGGCAGCTACCGGTTCACC
GACTTCCGCAGCCGCAAAACCGCACCTAAGGACAACGGACTGCGCAAAATCACGGTGCTAGCTAATGCAAAAGACGCTAA
GAAACAGAGCGCGCACGGCGCCACTGTGGCAAGCGCGGTTGCCACCGCCCGTGACCTGGTTAACACTCCCCCAAGCCACT
TGTTTCCCGCCGAATTTGCAAAATGCGCAAAGACTTTGGGCGAATCCGCTGGTCTTGAAGTGGAGGTGCTCGACGATAAG
GCGTTACAGAAAGCCGGCTACGGCGGGGTGATCGGTGTCGGTCAAGGTTCATCGCGTACGCCGCGGCTGGTGCGGCTGAT
TCATCAGGGATCAAGACTGGCTAAGAACCCCAAAAATGCCAGGAAGGTGGCCCTGGTCGGTAAAGGCATCACCTTTGACA
CCGGCGGCATATCGATCAAGCCAGCGGCGTCGATGCACCATATGACCTCGGATATGGCTGGAGCCGCCGCGGTCATCGCG
ACCATCACGCTGGCCGCACAATTGAAGCTGCCGATTGACGTAGTCGCTACCGTACCAATGGCTGAGAACATGCCGTCGGG
AACGGCGCAGCGGCCAGGCGACGTGCTGACGCAATACGGCGGAATCACAGTCGAGGTGCTCAACACCGACGCTGAAGGCC
GGCTTATCCTGGCCGACGCCATTGTTCGGGCATGCCAAGACAACCCGGACTACCTGATCGAGACATCCACCCTGACCGGT
GCGCAGACAGTGGCGCTGGGCTCCCGCATACCCGGGGTGATGGGCAGCGACAAGTTCCGCGACCGGGTTGCCAAGACCTC
GCAGCAGGTCGGCGAGAACGGCTGGCCGATGCCGCTGCCCGACGAACTCAAGGATGACTTGAAATCGACAGTCGCCGACC
TGGCCAATGTTAGCGGGCAACGTTTCGCCGGAATGCTGGTGGCTGGCGCGTTCCTCCGCGAATTCGTCGCCGACGGAGTG
GATTGGGCGCACATAGATATAGCCGGCCCGGCATACAACACCGACAGCCCGTGGGGGTATACCCCCAAAGGCGCCACCGG
TGTGCCAACCCGGACCATGTTCGCAATGCTCGAGGATATCGCCAACCACGGATAA

Upstream 100 bases:

>100_bases
TCAGCTACCTGGATGAAACTTCACGGCCGACGGCAGCCCAGATTGATTGGGCTTGAATTAGCAGACGCCGAGCTAATCTC
TGCCCCGGTGACTAGGGTGA

Downstream 100 bases:

>100_bases
ACCGCTTCGCCGACGACCAAGCTGGCTAGATGAGGGAAGATCAACCAGGCGCGAGCTCAGAGCGGACAGGTTAGCCGGAT
CGTCTCGCGGCTCCAACCTG

Product: leucyl aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MPVTTDPGYQGPSVHVAASLPKHDVSSSVLIVPVVSASDSDESEDRPGAVVAAAEPFLTAAAVAEIEAGLRALEATGGSD
QVHRLVAPSLPVGSVLTVSLGKPRSEWPADTIRRAAGVAARSLGRAEVVITTLAELPGESGASICSAVVEGLMLGSYRFT
DFRSRKTAPKDNGLRKITVLANAKDAKKQSAHGATVASAVATARDLVNTPPSHLFPAEFAKCAKTLGESAGLEVEVLDDK
ALQKAGYGGVIGVGQGSSRTPRLVRLIHQGSRLAKNPKNARKVALVGKGITFDTGGISIKPAASMHHMTSDMAGAAAVIA
TITLAAQLKLPIDVVATVPMAENMPSGTAQRPGDVLTQYGGITVEVLNTDAEGRLILADAIVRACQDNPDYLIETSTLTG
AQTVALGSRIPGVMGSDKFRDRVAKTSQQVGENGWPMPLPDELKDDLKSTVADLANVSGQRFAGMLVAGAFLREFVADGV
DWAHIDIAGPAYNTDSPWGYTPKGATGVPTRTMFAMLEDIANHG

Sequences:

>Translated_524_residues
MPVTTDPGYQGPSVHVAASLPKHDVSSSVLIVPVVSASDSDESEDRPGAVVAAAEPFLTAAAVAEIEAGLRALEATGGSD
QVHRLVAPSLPVGSVLTVSLGKPRSEWPADTIRRAAGVAARSLGRAEVVITTLAELPGESGASICSAVVEGLMLGSYRFT
DFRSRKTAPKDNGLRKITVLANAKDAKKQSAHGATVASAVATARDLVNTPPSHLFPAEFAKCAKTLGESAGLEVEVLDDK
ALQKAGYGGVIGVGQGSSRTPRLVRLIHQGSRLAKNPKNARKVALVGKGITFDTGGISIKPAASMHHMTSDMAGAAAVIA
TITLAAQLKLPIDVVATVPMAENMPSGTAQRPGDVLTQYGGITVEVLNTDAEGRLILADAIVRACQDNPDYLIETSTLTG
AQTVALGSRIPGVMGSDKFRDRVAKTSQQVGENGWPMPLPDELKDDLKSTVADLANVSGQRFAGMLVAGAFLREFVADGV
DWAHIDIAGPAYNTDSPWGYTPKGATGVPTRTMFAMLEDIANHG
>Mature_523_residues
PVTTDPGYQGPSVHVAASLPKHDVSSSVLIVPVVSASDSDESEDRPGAVVAAAEPFLTAAAVAEIEAGLRALEATGGSDQ
VHRLVAPSLPVGSVLTVSLGKPRSEWPADTIRRAAGVAARSLGRAEVVITTLAELPGESGASICSAVVEGLMLGSYRFTD
FRSRKTAPKDNGLRKITVLANAKDAKKQSAHGATVASAVATARDLVNTPPSHLFPAEFAKCAKTLGESAGLEVEVLDDKA
LQKAGYGGVIGVGQGSSRTPRLVRLIHQGSRLAKNPKNARKVALVGKGITFDTGGISIKPAASMHHMTSDMAGAAAVIAT
ITLAAQLKLPIDVVATVPMAENMPSGTAQRPGDVLTQYGGITVEVLNTDAEGRLILADAIVRACQDNPDYLIETSTLTGA
QTVALGSRIPGVMGSDKFRDRVAKTSQQVGENGWPMPLPDELKDDLKSTVADLANVSGQRFAGMLVAGAFLREFVADGVD
WAHIDIAGPAYNTDSPWGYTPKGATGVPTRTMFAMLEDIANHG

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family

Homologues:

Organism=Homo sapiens, GI41393561, Length=378, Percent_Identity=39.4179894179894, Blast_Score=235, Evalue=9e-62,
Organism=Homo sapiens, GI47155554, Length=344, Percent_Identity=32.8488372093023, Blast_Score=151, Evalue=2e-36,
Organism=Escherichia coli, GI1790710, Length=368, Percent_Identity=38.3152173913043, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082123, Length=366, Percent_Identity=35.792349726776, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17556903, Length=348, Percent_Identity=31.6091954022989, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17565172, Length=220, Percent_Identity=26.8181818181818, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24662227, Length=422, Percent_Identity=28.1990521327014, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24661038, Length=327, Percent_Identity=30.5810397553517, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI21355725, Length=417, Percent_Identity=29.0167865707434, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI221379063, Length=308, Percent_Identity=34.4155844155844, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI221379062, Length=308, Percent_Identity=34.4155844155844, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21357381, Length=308, Percent_Identity=34.4155844155844, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI20129969, Length=422, Percent_Identity=29.1469194312796, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI161077148, Length=423, Percent_Identity=28.8416075650118, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI20130057, Length=423, Percent_Identity=28.8416075650118, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21355645, Length=425, Percent_Identity=27.7647058823529, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24662223, Length=425, Percent_Identity=27.7647058823529, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129963, Length=423, Percent_Identity=29.0780141843972, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI19922386, Length=334, Percent_Identity=28.1437125748503, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24646701, Length=219, Percent_Identity=34.2465753424658, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24646703, Length=219, Percent_Identity=34.2465753424658, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21358201, Length=219, Percent_Identity=34.2465753424658, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPA_MYCLE (O32956)

Other databases:

- EMBL:   Z98741
- EMBL:   AL583920
- PIR:   T44889
- RefSeq:   NP_301652.1
- ProteinModelPortal:   O32956
- SMR:   O32956
- EnsemblBacteria:   EBMYCT00000028376
- GeneID:   909860
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0864
- NMPDR:   fig|272631.1.peg.524
- Leproma:   ML0864
- GeneTree:   EBGT00050000016870
- HOGENOM:   HBG742580
- OMA:   ELHKPCE
- ProtClustDB:   PRK00913
- BioCyc:   MLEP272631:ML0864-MONOMER
- BRENDA:   3.4.11.1
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00181
- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283
- PANTHER:   PTHR11963:SF3
- PRINTS:   PR00481

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N

EC number: =3.4.11.1; =3.4.11.10

Molecular weight: Translated: 54342; Mature: 54211

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00631 CYTOSOL_AP; PS00277 STAPH_STREP_TOXIN_1

Important sites: ACT_SITE 300-300 ACT_SITE 374-374

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTTDPGYQGPSVHVAASLPKHDVSSSVLIVPVVSASDSDESEDRPGAVVAAAEPFLTA
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCHHHHH
AAVAEIEAGLRALEATGGSDQVHRLVAPSLPVGSVLTVSLGKPRSEWPADTIRRAAGVAA
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
RSLGRAEVVITTLAELPGESGASICSAVVEGLMLGSYRFTDFRSRKTAPKDNGLRKITVL
HHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEECCHHHCCCCCCCCCCCEEEEEE
ANAKDAKKQSAHGATVASAVATARDLVNTPPSHLFPAEFAKCAKTLGESAGLEVEVLDDK
ECCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCH
ALQKAGYGGVIGVGQGSSRTPRLVRLIHQGSRLAKNPKNARKVALVGKGITFDTGGISIK
HHHHCCCCCEEEECCCCCCCHHHHHHHHHCHHHHCCCCCCCEEEEEECCEEECCCCEEEC
PAASMHHMTSDMAGAAAVIATITLAAQLKLPIDVVATVPMAENMPSGTAQRPGDVLTQYG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCHHHHHHCC
GITVEVLNTDAEGRLILADAIVRACQDNPDYLIETSTLTGAQTVALGSRIPGVMGSDKFR
CEEEEEEECCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCHHHH
DRVAKTSQQVGENGWPMPLPDELKDDLKSTVADLANVSGQRFAGMLVAGAFLREFVADGV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DWAHIDIAGPAYNTDSPWGYTPKGATGVPTRTMFAMLEDIANHG
CEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVTTDPGYQGPSVHVAASLPKHDVSSSVLIVPVVSASDSDESEDRPGAVVAAAEPFLTA
CCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCHHHHH
AAVAEIEAGLRALEATGGSDQVHRLVAPSLPVGSVLTVSLGKPRSEWPADTIRRAAGVAA
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
RSLGRAEVVITTLAELPGESGASICSAVVEGLMLGSYRFTDFRSRKTAPKDNGLRKITVL
HHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEECCHHHCCCCCCCCCCCEEEEEE
ANAKDAKKQSAHGATVASAVATARDLVNTPPSHLFPAEFAKCAKTLGESAGLEVEVLDDK
ECCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCH
ALQKAGYGGVIGVGQGSSRTPRLVRLIHQGSRLAKNPKNARKVALVGKGITFDTGGISIK
HHHHCCCCCEEEECCCCCCCHHHHHHHHHCHHHHCCCCCCCEEEEEECCEEECCCCEEEC
PAASMHHMTSDMAGAAAVIATITLAAQLKLPIDVVATVPMAENMPSGTAQRPGDVLTQYG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCHHHHHHCC
GITVEVLNTDAEGRLILADAIVRACQDNPDYLIETSTLTGAQTVALGSRIPGVMGSDKFR
CEEEEEEECCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCHHHH
DRVAKTSQQVGENGWPMPLPDELKDDLKSTVADLANVSGQRFAGMLVAGAFLREFVADGV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DWAHIDIAGPAYNTDSPWGYTPKGATGVPTRTMFAMLEDIANHG
CEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002