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The map label for this gene is sucB [H]

Identifier: 221229863

GI number: 221229863

Start: 1023164

End: 1024756

Strand: Reverse

Name: sucB [H]

Synonym: MLBr_00861

Alternate gene names: 221229863

Gene position: 1024756-1023164 (Counterclockwise)

Preceding gene: 221229866

Following gene: 221229862

Centisome position: 31.36

GC content: 61.71

Gene sequence:

>1593_bases
ATGGCCTGCTCCGTCCAGATGCCGGCACTCGGTGAAAGCGTCACCGAGGGGACAGTTACCCGCTGGCTCAAACAGGAAGG
CGACACGGTCGAACTCGACGAGCCACTCGTCGAGGTGTCGACCGATAAGGTCGACACCGAAATCCCCTCGCCTGCCGCGG
GTGTGCTGACCAAAATCATCGCCCAGGAGGACGACACCGTTGAAGTGGGCGGTGAACTTGCCGTTATCGGCGCGCCTTCC
GAAGCGGCTGCAGCGGCGCCTGCGCCCCGGCCAGAACCTAAGGCCCAACCCGAACCCGCAGCATCATCCCAACCTGCCGC
ACCGGCCCAGCAACCTTCCGGCGCAGCAACCGCGACACCGGTTCTGATGCCCGAGCTCGGCGAGTCTGTAACCGAAGGCA
CGGTGACTCGCTGGCTTAAGAAGATCGGAGACTCGGTTCAGGCTGACGAGCCACTGGTAGAGGTATCGACCGACAAGGTA
GATACCGAGATCCCGTCACCGGTGGCCGGCGTTTTGGTCAGCATCACCACCAACGAAGACACAACCGTCCCGGTTGGCGG
TGAGTTGGCACGGATAGGTGTTACTCTCGACAGCATCGCCACCCCCGCGCCAGCGCCCAGAGCCGAATCCGTACCGTCCC
GGCCGACGCCAGCCAGGAAAGAAGCCAATGGCGCACCGTACGTGACCCCGCTGGTACGAAAACTCGCCACCGAAAACAAC
ATCGACCTGGCCAAAGTGATAGGCACCGGTGTGGGCGGTCGCATCCGTAAGCAGGACGTGCTGGCCGCGGCCGAACAACG
GAAACAGCAGCAGGCACCGACATCCGCGCCATCAGCAGCCGCTCCCACCCCGACACCCGTGCTGGCCCACTTGCGAGGCA
CCACCCAAAAGGTCAGCCGGATTCGGCAAATCACCGCGAAAAAGACCCGCGAATCCCTGCAGGCCACGGCGCAACTCACC
CAGACCCATGAGGTCGATATGGCCAAGATTGTGGGGTTGCGAGCCAAAGCCAAGGCAGCTTTCGCCGAGCGCGAAGGGGT
GAACCTGACTTTCCTGCCGTTTATCGCCAAGGCAGCGATCGACGCCCTCAAAATTCATCCCAATATTAACGCCAGCTACA
ACGAGGACACTAAGGAGATCACCTATTACGACGCCGAGCACCTCGGCTTCGCAATCGACACTGACAAGGGCCTGCTCTCC
CCTGTTATCCACTATGCCGGTGATTTGTCGCTGGCCGGGCTGGCCCGCGCAATTGTTGATATCGCCGCCCGGGCTCGGTC
AGGCAATTTGAAACCCGAGGAGCTGTCCGGTGGCACATTCACCATTACCAACATCGGCAGCCAGGGCGCGTTGTTCGACA
CACCGATCCTGGTTCCGCCGCAGGCAGCAATGCTGGGCATCGGAGCCATCGTAAAACGCCCGCGGGTGGTCATCGATGCT
AGCGGTAATGAGTCGATTGGGGTCCGCGCGATTTGCTATCTGCCGCTGACCTATGACCACCGACTGATCGACGGCGCCGA
TGCCGGACGTTTCCTCACTACCATCAAGCACCGGCTCGAAGAGGGAGCATTCGAGGCCGACCTAGGTCTTTAG

Upstream 100 bases:

>100_bases
TGGTTCCGATATCCACTGGGTCCCCCGCCCGGTACACCGCGGTGTGGTAAGCCAGATGTCGACCTGCTCCTAACTATGAG
CGATCGAGGAGTCAAAACAG

Downstream 100 bases:

>100_bases
GAGAGGCTTGCCAACGTGGCTCAAGCTAGTCGTAAGGCCGTTGTCGCGATAGCGGGTTCGTCCGGCATGATCGGCTCTGC
TTTGGCCGCGGCGCTGCGCG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MACSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGAPS
EAAAAAPAPRPEPKAQPEPAASSQPAAPAQQPSGAATATPVLMPELGESVTEGTVTRWLKKIGDSVQADEPLVEVSTDKV
DTEIPSPVAGVLVSITTNEDTTVPVGGELARIGVTLDSIATPAPAPRAESVPSRPTPARKEANGAPYVTPLVRKLATENN
IDLAKVIGTGVGGRIRKQDVLAAAEQRKQQQAPTSAPSAAAPTPTPVLAHLRGTTQKVSRIRQITAKKTRESLQATAQLT
QTHEVDMAKIVGLRAKAKAAFAEREGVNLTFLPFIAKAAIDALKIHPNINASYNEDTKEITYYDAEHLGFAIDTDKGLLS
PVIHYAGDLSLAGLARAIVDIAARARSGNLKPEELSGGTFTITNIGSQGALFDTPILVPPQAAMLGIGAIVKRPRVVIDA
SGNESIGVRAICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL

Sequences:

>Translated_530_residues
MACSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGAPS
EAAAAAPAPRPEPKAQPEPAASSQPAAPAQQPSGAATATPVLMPELGESVTEGTVTRWLKKIGDSVQADEPLVEVSTDKV
DTEIPSPVAGVLVSITTNEDTTVPVGGELARIGVTLDSIATPAPAPRAESVPSRPTPARKEANGAPYVTPLVRKLATENN
IDLAKVIGTGVGGRIRKQDVLAAAEQRKQQQAPTSAPSAAAPTPTPVLAHLRGTTQKVSRIRQITAKKTRESLQATAQLT
QTHEVDMAKIVGLRAKAKAAFAEREGVNLTFLPFIAKAAIDALKIHPNINASYNEDTKEITYYDAEHLGFAIDTDKGLLS
PVIHYAGDLSLAGLARAIVDIAARARSGNLKPEELSGGTFTITNIGSQGALFDTPILVPPQAAMLGIGAIVKRPRVVIDA
SGNESIGVRAICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
>Mature_529_residues
ACSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGAPSE
AAAAAPAPRPEPKAQPEPAASSQPAAPAQQPSGAATATPVLMPELGESVTEGTVTRWLKKIGDSVQADEPLVEVSTDKVD
TEIPSPVAGVLVSITTNEDTTVPVGGELARIGVTLDSIATPAPAPRAESVPSRPTPARKEANGAPYVTPLVRKLATENNI
DLAKVIGTGVGGRIRKQDVLAAAEQRKQQQAPTSAPSAAAPTPTPVLAHLRGTTQKVSRIRQITAKKTRESLQATAQLTQ
THEVDMAKIVGLRAKAKAAFAEREGVNLTFLPFIAKAAIDALKIHPNINASYNEDTKEITYYDAEHLGFAIDTDKGLLSP
VIHYAGDLSLAGLARAIVDIAARARSGNLKPEELSGGTFTITNIGSQGALFDTPILVPPQAAMLGIGAIVKRPRVVIDAS
GNESIGVRAICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=31.042654028436, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI31711992, Length=562, Percent_Identity=29.3594306049822, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI19923748, Length=251, Percent_Identity=38.6454183266932, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI203098816, Length=473, Percent_Identity=26.215644820296, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=26.1160714285714, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=36.7647058823529, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1786305, Length=539, Percent_Identity=30.7977736549165, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=414, Percent_Identity=31.6425120772947, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=29.2857142857143, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=30.316742081448, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=324, Percent_Identity=27.4691358024691, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320352, Length=417, Percent_Identity=32.6139088729017, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324258, Length=444, Percent_Identity=27.027027027027, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=28.169014084507, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=36, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24582497, Length=299, Percent_Identity=27.7591973244147, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20129315, Length=299, Percent_Identity=27.7591973244147, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 55473; Mature: 55342

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHEECCCCCCCCCCCCHHHHHHHHH
AQEDDTVEVGGELAVIGAPSEAAAAAPAPRPEPKAQPEPAASSQPAAPAQQPSGAATATP
HCCCCCEEECCEEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VLMPELGESVTEGTVTRWLKKIGDSVQADEPLVEVSTDKVDTEIPSPVAGVLVSITTNED
CHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCC
TTVPVGGELARIGVTLDSIATPAPAPRAESVPSRPTPARKEANGAPYVTPLVRKLATENN
CEECCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
IDLAKVIGTGVGGRIRKQDVLAAAEQRKQQQAPTSAPSAAAPTPTPVLAHLRGTTQKVSR
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHH
IRQITAKKTRESLQATAQLTQTHEVDMAKIVGLRAKAKAAFAEREGVNLTFLPFIAKAAI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
DALKIHPNINASYNEDTKEITYYDAEHLGFAIDTDKGLLSPVIHYAGDLSLAGLARAIVD
HHEEECCCCCCCCCCCCCEEEEEEHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHH
IAARARSGNLKPEELSGGTFTITNIGSQGALFDTPILVPPQAAMLGIGAIVKRPRVVIDA
HHHHHCCCCCCHHHCCCCEEEEEECCCCCCEECCCEECCCHHHHHHHHHHHCCCEEEEEC
SGNESIGVRAICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
CCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
ACSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHEECCCCCCCCCCCCHHHHHHHHH
AQEDDTVEVGGELAVIGAPSEAAAAAPAPRPEPKAQPEPAASSQPAAPAQQPSGAATATP
HCCCCCEEECCEEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VLMPELGESVTEGTVTRWLKKIGDSVQADEPLVEVSTDKVDTEIPSPVAGVLVSITTNED
CHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCC
TTVPVGGELARIGVTLDSIATPAPAPRAESVPSRPTPARKEANGAPYVTPLVRKLATENN
CEECCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
IDLAKVIGTGVGGRIRKQDVLAAAEQRKQQQAPTSAPSAAAPTPTPVLAHLRGTTQKVSR
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHH
IRQITAKKTRESLQATAQLTQTHEVDMAKIVGLRAKAKAAFAEREGVNLTFLPFIAKAAI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
DALKIHPNINASYNEDTKEITYYDAEHLGFAIDTDKGLLSPVIHYAGDLSLAGLARAIVD
HHEEECCCCCCCCCCCCCEEEEEEHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHH
IAARARSGNLKPEELSGGTFTITNIGSQGALFDTPILVPPQAAMLGIGAIVKRPRVVIDA
HHHHHCCCCCCHHHCCCCEEEEEECCCCCCEECCCEECCCHHHHHHHHHHHCCCEEEEEC
SGNESIGVRAICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
CCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]