Click here to switch to the map view.

The map label for this gene is narK [H]

Identifier: 221229851

GI number: 221229851

Start: 1002602

End: 1004155

Strand: Direct

Name: narK [H]

Synonym: MLBr_00844

Alternate gene names: 221229851

Gene position: 1002602-1004155 (Clockwise)

Preceding gene: 221229850

Following gene: 221229853

Centisome position: 30.68

GC content: 59.52

Gene sequence:

>1554_bases
ATGGATCAGCTGGTGTTGCTGCAAGCCGAGGAGTCGCTGTACGGCCCCGGTCGCAGATCGAAAACTGTACCGATTGACCA
TCGGCACAGTCTAAAGTCGAGCTTTTTCCGGCTGCGTTTGAGGAGAGCGTGTCGCATCTCGCATTGGGACCCCGAGGATC
AAGCGGCTTGGGAGGCTGGCAACAAAACCATCGCCCGGCTCAATCTGCTCTGGTCGGTCCTGACCGTCCACCTGGGGTAT
TCGGTGTGGACGCTCTGGCCGGTGATGGAGCTGTTCATGCCCAAGGACGTATATGGCTTTTCCGCGGGTGACAAGTTTCT
GCTCGCTACCACCGCAACCTTGGTTGGGGCGTGCCTGCGGGTGCCGTACTCGTTGGCCACCGCACTTTTCGGTGGCCGCA
ACTGGGCGATTTTCTCGGTGATGGTGCTGTTGATCCCCACTATTGCCACCATGGTGCTGTTGGCTCATCCAGGCTTGCCG
CTTTGGCCTTATCTGGCGTGCGCGGCACTGACCGGCTTGGGCGGCGGTAATTTCGCGGCGTCGATGACTAACGCCAACGC
CTTCTACCCGCATCGGCTCAAGGGTGCGGCATTCGGGCTCGCTGGTGGGGCCGGCAACCTTGGGGTGTCGGTGATCCAGG
TGGTCGGGATGCTGGTCATCGCCAGCGTCGGTGACCGAAAGCCTTACATTGTGTGTGGACTTTATGTGGTGCTGTTGATA
ATCGCCGGGATCGGAGCAATGTTGTTCATGAACGACATTGAGCATCACCGTATCGGGGTGAACACCATCCGGCCAGTACT
GTTCGTCGTGGTTTCCACGCGTGATAGCTGGGTTCTCGCGCTGCTCTACCTGGCGAGTTTCGGTTCGTTCATCGGTTTTT
CATTCGCATTCGGCCAGGTGCTGGAAACCAACTTCGTCGCCGGCGGACAAAGTACGGCTCAGGCCGCGTTGCACGCCGCT
GAGCTTGCATTCATCGGACCGACGTTGGCCGCGGTAGCCCGGTTTTGGGGTGGCCGACTGGCCGACCGACTTGGAGGGAG
TCGCGTCACCCTGGTGGTCTTCGGTGCCATGGTTTTTGCCGCCGGGTTGCTGGGTGTACTGGGCATAATTGAGGGCTCAC
GCGTGTGCCCGATCAGGGGTGTCATGATGGCCAGCTACTTCGCTGGATTCATCACTCTGTTCATCTTGTCTGGGTTGGGC
AATGGATCGGTGTACAAGATGATCCCGACAATTTTCGAGGCCTGCAGCCATTCCCTGGGAATCAACGACGACGAGTGCCG
CGACTGGTCACGAGTGATTTCCGGGGTCGTCATTGGCTTTGTCGCGGAGGTCGGCGCACTCGGCGGTGTTGGGATCGACC
TGGCGTTGCGAGAGTCCTACCTCAACACTGGCGGGGTTACCGCAGCGTTTTGGATATTCATGTTGTGCTATGCCGCGGCC
GGTGTCCTGACCTGGAAGATGTACGTGTGTCGACCATTGCCAGGCAACTTGCACGACGAAGCGGCTAATGCGTTTGCGGC
CAGCGTCGGAGCCAGCAGAACTCACCGGGGCTGA

Upstream 100 bases:

>100_bases
TTCTACAGGAGGTTCAGGCTCTCCGGGATGCGTTTCGCGCCCCGAAGCCTGTCACCCTTGGGCGTCCGTGGCTTTAGGCG
CAGCTGGGTTAAGGAGGGTG

Downstream 100 bases:

>100_bases
AGCTCAGACGGGCAGATTCTCGAAGTGCGGCATCTCGGCGTAACGCAACTGCTTTGGGACTCCGAAAACCGTCGACCAAC
GTCTCGGCATAGGGCTGCAG

Product: putative nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MDQLVLLQAEESLYGPGRRSKTVPIDHRHSLKSSFFRLRLRRACRISHWDPEDQAAWEAGNKTIARLNLLWSVLTVHLGY
SVWTLWPVMELFMPKDVYGFSAGDKFLLATTATLVGACLRVPYSLATALFGGRNWAIFSVMVLLIPTIATMVLLAHPGLP
LWPYLACAALTGLGGGNFAASMTNANAFYPHRLKGAAFGLAGGAGNLGVSVIQVVGMLVIASVGDRKPYIVCGLYVVLLI
IAGIGAMLFMNDIEHHRIGVNTIRPVLFVVVSTRDSWVLALLYLASFGSFIGFSFAFGQVLETNFVAGGQSTAQAALHAA
ELAFIGPTLAAVARFWGGRLADRLGGSRVTLVVFGAMVFAAGLLGVLGIIEGSRVCPIRGVMMASYFAGFITLFILSGLG
NGSVYKMIPTIFEACSHSLGINDDECRDWSRVISGVVIGFVAEVGALGGVGIDLALRESYLNTGGVTAAFWIFMLCYAAA
GVLTWKMYVCRPLPGNLHDEAANAFAASVGASRTHRG

Sequences:

>Translated_517_residues
MDQLVLLQAEESLYGPGRRSKTVPIDHRHSLKSSFFRLRLRRACRISHWDPEDQAAWEAGNKTIARLNLLWSVLTVHLGY
SVWTLWPVMELFMPKDVYGFSAGDKFLLATTATLVGACLRVPYSLATALFGGRNWAIFSVMVLLIPTIATMVLLAHPGLP
LWPYLACAALTGLGGGNFAASMTNANAFYPHRLKGAAFGLAGGAGNLGVSVIQVVGMLVIASVGDRKPYIVCGLYVVLLI
IAGIGAMLFMNDIEHHRIGVNTIRPVLFVVVSTRDSWVLALLYLASFGSFIGFSFAFGQVLETNFVAGGQSTAQAALHAA
ELAFIGPTLAAVARFWGGRLADRLGGSRVTLVVFGAMVFAAGLLGVLGIIEGSRVCPIRGVMMASYFAGFITLFILSGLG
NGSVYKMIPTIFEACSHSLGINDDECRDWSRVISGVVIGFVAEVGALGGVGIDLALRESYLNTGGVTAAFWIFMLCYAAA
GVLTWKMYVCRPLPGNLHDEAANAFAASVGASRTHRG
>Mature_517_residues
MDQLVLLQAEESLYGPGRRSKTVPIDHRHSLKSSFFRLRLRRACRISHWDPEDQAAWEAGNKTIARLNLLWSVLTVHLGY
SVWTLWPVMELFMPKDVYGFSAGDKFLLATTATLVGACLRVPYSLATALFGGRNWAIFSVMVLLIPTIATMVLLAHPGLP
LWPYLACAALTGLGGGNFAASMTNANAFYPHRLKGAAFGLAGGAGNLGVSVIQVVGMLVIASVGDRKPYIVCGLYVVLLI
IAGIGAMLFMNDIEHHRIGVNTIRPVLFVVVSTRDSWVLALLYLASFGSFIGFSFAFGQVLETNFVAGGQSTAQAALHAA
ELAFIGPTLAAVARFWGGRLADRLGGSRVTLVVFGAMVFAAGLLGVLGIIEGSRVCPIRGVMMASYFAGFITLFILSGLG
NGSVYKMIPTIFEACSHSLGINDDECRDWSRVISGVVIGFVAEVGALGGVGIDLALRESYLNTGGVTAAFWIFMLCYAAA
GVLTWKMYVCRPLPGNLHDEAANAFAASVGASRTHRG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=462, Percent_Identity=35.0649350649351, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787743, Length=464, Percent_Identity=35.3448275862069, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55251; Mature: 55251

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQLVLLQAEESLYGPGRRSKTVPIDHRHSLKSSFFRLRLRRACRISHWDPEDQAAWEAG
CCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
NKTIARLNLLWSVLTVHLGYSVWTLWPVMELFMPKDVYGFSAGDKFLLATTATLVGACLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VPYSLATALFGGRNWAIFSVMVLLIPTIATMVLLAHPGLPLWPYLACAALTGLGGGNFAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEE
SMTNANAFYPHRLKGAAFGLAGGAGNLGVSVIQVVGMLVIASVGDRKPYIVCGLYVVLLI
EECCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IAGIGAMLFMNDIEHHRIGVNTIRPVLFVVVSTRDSWVLALLYLASFGSFIGFSFAFGQV
HHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LETNFVAGGQSTAQAALHAAELAFIGPTLAAVARFWGGRLADRLGGSRVTLVVFGAMVFA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHH
AGLLGVLGIIEGSRVCPIRGVMMASYFAGFITLFILSGLGNGSVYKMIPTIFEACSHSLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
INDDECRDWSRVISGVVIGFVAEVGALGGVGIDLALRESYLNTGGVTAAFWIFMLCYAAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GVLTWKMYVCRPLPGNLHDEAANAFAASVGASRTHRG
HHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDQLVLLQAEESLYGPGRRSKTVPIDHRHSLKSSFFRLRLRRACRISHWDPEDQAAWEAG
CCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
NKTIARLNLLWSVLTVHLGYSVWTLWPVMELFMPKDVYGFSAGDKFLLATTATLVGACLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VPYSLATALFGGRNWAIFSVMVLLIPTIATMVLLAHPGLPLWPYLACAALTGLGGGNFAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEE
SMTNANAFYPHRLKGAAFGLAGGAGNLGVSVIQVVGMLVIASVGDRKPYIVCGLYVVLLI
EECCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IAGIGAMLFMNDIEHHRIGVNTIRPVLFVVVSTRDSWVLALLYLASFGSFIGFSFAFGQV
HHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LETNFVAGGQSTAQAALHAAELAFIGPTLAAVARFWGGRLADRLGGSRVTLVVFGAMVFA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHH
AGLLGVLGIIEGSRVCPIRGVMMASYFAGFITLFILSGLGNGSVYKMIPTIFEACSHSLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
INDDECRDWSRVISGVVIGFVAEVGALGGVGIDLALRESYLNTGGVTAAFWIFMLCYAAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GVLTWKMYVCRPLPGNLHDEAANAFAASVGASRTHRG
HHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]