Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 221229759

GI number: 221229759

Start: 807515

End: 807997

Strand: Direct

Name: smpB

Synonym: MLBr_00671

Alternate gene names: 221229759

Gene position: 807515-807997 (Clockwise)

Preceding gene: 221229758

Following gene: 221229760

Centisome position: 24.71

GC content: 60.25

Gene sequence:

>483_bases
GTGGCCAGAAACCCTCGTGGCGGCAAGCAGATTGTGGCCACCAATCGCAAGGCTCGGCACGATTACGCAATCATTGAATT
GTTTGAGGCTGGGGTGGCGTTGCTTGGTACCGAGGTGAAAAGTCTGCGGGAGGGGCATGCGTCGCTGGCCGACGCGTTCG
CAACCGTCGACAGCGGTGAAGTGTGGTTGCGAAACATGCACATCCCGGAGTATCAGCATGGTAGCTGGACCAATCACGAT
CCTCGCCGTAACCGCAAGCTGCTGCTGCACCGCCGCCAGATCGACACCCTGGTTGGCAAGATCCGTGACGGCAACCTCGC
GCTAGTGCCGTTATCGTTGTATTTTGCCGAGGGCAAGGTCAAGGTCGAGCTTGCGCTGGCGCGCGGCAAGAAACTGCACG
ACAAGCGCCAGGACATGGCGCGTCGCGATGCTCAGCGCGAAGTAATCCGTGAACTCGGTCGCCGTGCCAAGGGCATGCTC
TGA

Upstream 100 bases:

>100_bases
CCGATGTCCTGTACGTTTCCCCGTGGTTGTTGTTGCTCGGCGTGGCGTTGGCCGCGTTGACCGGTTACGCAACATTGCGC
ATTTACGTGCGACGGTAAGT

Downstream 100 bases:

>100_bases
GCGAGATCGGCCTAATATCCGACCTATGGTCGTCTGTTTCACCCCCCAACTTGACACCCGACGTGTTGTCCGGGTCGGTT
CACTGCGTGGGACTACCAAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MARNPRGGKQIVATNRKARHDYAIIELFEAGVALLGTEVKSLREGHASLADAFATVDSGEVWLRNMHIPEYQHGSWTNHD
PRRNRKLLLHRRQIDTLVGKIRDGNLALVPLSLYFAEGKVKVELALARGKKLHDKRQDMARRDAQREVIRELGRRAKGML

Sequences:

>Translated_160_residues
MARNPRGGKQIVATNRKARHDYAIIELFEAGVALLGTEVKSLREGHASLADAFATVDSGEVWLRNMHIPEYQHGSWTNHD
PRRNRKLLLHRRQIDTLVGKIRDGNLALVPLSLYFAEGKVKVELALARGKKLHDKRQDMARRDAQREVIRELGRRAKGML
>Mature_159_residues
ARNPRGGKQIVATNRKARHDYAIIELFEAGVALLGTEVKSLREGHASLADAFATVDSGEVWLRNMHIPEYQHGSWTNHDP
RRNRKLLLHRRQIDTLVGKIRDGNLALVPLSLYFAEGKVKVELALARGKKLHDKRQDMARRDAQREVIRELGRRAKGML

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=139, Percent_Identity=43.1654676258993, Blast_Score=113, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_MYCLB (B8ZUV3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503175.1
- ProteinModelPortal:   B8ZUV3
- SMR:   B8ZUV3
- EnsemblBacteria:   EBMYCT00000086092
- GeneID:   7325316
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017126
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18205; Mature: 18074

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNPRGGKQIVATNRKARHDYAIIELFEAGVALLGTEVKSLREGHASLADAFATVDSGE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VWLRNMHIPEYQHGSWTNHDPRRNRKLLLHRRQIDTLVGKIRDGNLALVPLSLYFAEGKV
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCEE
KVELALARGKKLHDKRQDMARRDAQREVIRELGRRAKGML
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARNPRGGKQIVATNRKARHDYAIIELFEAGVALLGTEVKSLREGHASLADAFATVDSGE
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VWLRNMHIPEYQHGSWTNHDPRRNRKLLLHRRQIDTLVGKIRDGNLALVPLSLYFAEGKV
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCEE
KVELALARGKKLHDKRQDMARRDAQREVIRELGRRAKGML
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA