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The map label for this gene is folD

Identifier: 221229760

GI number: 221229760

Start: 810701

End: 811549

Strand: Direct

Name: folD

Synonym: MLBr_00674

Alternate gene names: 221229760

Gene position: 810701-811549 (Clockwise)

Preceding gene: 221229759

Following gene: 221229761

Centisome position: 24.81

GC content: 63.13

Gene sequence:

>849_bases
GTGGGTGCAATCACGCTGGACGGCAAGGCCACCCGAGACGAGATCCTGATCGACCTCAAGCAACGCGTGGCCGCATTAAC
CGAATCCGGTCGCACGCCCGGACTGGGTACCATCCTGGTCGGTGACGACCCCGGATCACATGCCTATGTTCGTGGCAAGC
ACGCCGACTGTGCGAAGGTGGGCATCACATCGATTCGCCGCGACTTGCCCGTCGACATCACAACGGCCGTGCTGCATGAC
ACCATCGAGGAACTGAATGCCAACCCCGACTGCACCGGCTATATCGTGCAGTTGCCGTTACCCAAGTACCTGGACGAGAA
CACGGCGCTGGAGCGTGTCGACCCGGCCAAGGATGCCGACGGCCTGCACCCGACGAACCTCGGCCGGCTAGTGCTGTCCA
CCCCGGCGCCGCTGCCGTGTACCGCGCGTGGCATTTTGCACCTGCTACGGCGTTACGGCGTCGAGATCGCCGGAACACAC
GTCGTCATCATCGGGCGCGGTGTTACGGTTGGTCGCCCGTTGGGGCTGCTGCTGACACGCCGTTCCGAGAACGCCACGGT
GACTTTGTGCCACACAGGAACGCGCAATCTGGCAGCGCTAACCAAGCAGGCTGACATCATTGTAGCCGCCGTCGGTGTTC
CACATCTGCTGACCGCGGATATGGTGCGTCCCGGAGCTGTGGTGGTCGACGTCGGTGTCAGCCGGGTCGAGACTAGGCTC
GTCGGCGACGTGCATCCGGATGTCTGGGAAGTCGCCGGTCACGTCTCACCGAATCCTGGTGGCGTTGGTCCGCTTACCCG
GGTGTTTCTGCTGACCAACGTTGTCGAATTGGCCGAGGGACGGCAGTAA

Upstream 100 bases:

>100_bases
CGGCTGAACACGTCCGTTGTGTTGGCCATATCTGGAGCCTAACCGCCTGCAGCGGGTCTTAAGCATACTGACGCCGGTAG
GCTATGTCAGACTGTCAGAC

Downstream 100 bases:

>100_bases
ACGTGCGTGCAGCGTTGCGGTCGCAATGGCCGATTCTGCTGGTTGGGATGATCTTCGCTGGAGCGTTCGTGCTGGTGGGG
GCAAATTTTTGGCGTCGTGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKVGITSIRRDLPVDITTAVLHD
TIEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPCTARGILHLLRRYGVEIAGTH
VVIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNLAALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVETRL
VGDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ

Sequences:

>Translated_282_residues
MGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKVGITSIRRDLPVDITTAVLHD
TIEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPCTARGILHLLRRYGVEIAGTH
VVIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNLAALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVETRL
VGDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ
>Mature_281_residues
GAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKVGITSIRRDLPVDITTAVLHDT
IEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPCTARGILHLLRRYGVEIAGTHV
VIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNLAALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVETRLV
GDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=39.9317406143345, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI94721354, Length=289, Percent_Identity=38.7543252595156, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=35.7638888888889, Blast_Score=158, Evalue=7e-39,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=40.5017921146953, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17568735, Length=298, Percent_Identity=33.2214765100671, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319558, Length=260, Percent_Identity=41.1538461538462, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=35.7615894039735, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=40, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=40, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=40.6896551724138, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=40.6896551724138, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=40.6896551724138, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=40.6896551724138, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_MYCLB (B8ZUV4)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503176.1
- ProteinModelPortal:   B8ZUV4
- SMR:   B8ZUV4
- EnsemblBacteria:   EBMYCT00000086815
- GeneID:   7327006
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016741
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK14193
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29836; Mature: 29705

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKV
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCHHHH
GITSIRRDLPVDITTAVLHDTIEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDAD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCCCCHHHCCCCCCCC
GLHPTNLGRLVLSTPAPLPCTARGILHLLRRYGVEIAGTHVVIIGRGVTVGRPLGLLLTR
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEECEEEEEEECCCCCCCCCEEEEEE
RSENATVTLCHTGTRNLAALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVETRL
CCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCHHHHHHHCCCCEEEEECCHHHHHHHH
VGDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ
HCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKV
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCHHHH
GITSIRRDLPVDITTAVLHDTIEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDAD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCCCCHHHCCCCCCCC
GLHPTNLGRLVLSTPAPLPCTARGILHLLRRYGVEIAGTHVVIIGRGVTVGRPLGLLLTR
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEECEEEEEEECCCCCCCCCEEEEEE
RSENATVTLCHTGTRNLAALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVETRL
CCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCHHHHHHHCCCCEEEEECCHHHHHHHH
VGDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ
HCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA