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The map label for this gene is priA

Identifier: 221229679

GI number: 221229679

Start: 664269

End: 666224

Strand: Direct

Name: priA

Synonym: MLBr_00548

Alternate gene names: 221229679

Gene position: 664269-666224 (Clockwise)

Preceding gene: 221229678

Following gene: 221229680

Centisome position: 20.33

GC content: 66.41

Gene sequence:

>1956_bases
ATGCTGTCGGTGCCGCATCTGGATCGCGAGTTTGATTACCTGGTATCCGCCGAGCAGTCTGACGATGCTCAACCGGGGGT
GCGGGTGCGGGTGCGGTTCCATGGTCGGCTGGTCGACGGGTTTGTGCTGGAGCGCCGTAATGACACAGAGCACTTCGGCA
AATTGGGCTGGCTGGATCGTGTAGTATCCGCTCAACCGGTACTCACCGCAGAAGTACGTCGGCTGGTCGACGCGGTAGTG
GCTCGCTATGCTGGGACTAGACCGGATGTGTTGCGGTTGGCGGTGCCAACTCGGCATGCCAGAGTGGAGCGAGAAACCCT
TGCGATCCCCGTGTCACCGCTCCCGTTGATTCCGGGGCCGGTCGACCCGTCGGGCTGGGAGGTTTACGGTCGTGGCGGGC
AGTTCCTGACTGCGCTGGCCGAGTCGCGCGCCGCCCGCGCCGTCTGGCAAGCGCTACCCGGCGAGCAATGGGCGGACCGA
TTTGCCGAGGCTGCCGCGCAAGCCATCCGCGCCGGACGGGCGGCATTGGCGATCGTGCCTGATCAACGGGATTTGGATGT
ACTGTGGCGGGCCGTGACGACTCGGGTCGACGAACGTAGTGTGGTGGCTCTCTCAGCGGGCCTGGGTCAGGCAGTGCGGT
ATCAGCGTTGGCTGAAGGTGTTGCGCGGCAGCGCGCGGCTAGTGATCGGCACCCGCAGTGCGGTGTTCGCGCCGGTCAAC
GACCTGGGTTTGGTCATGGTCTGGTCTGATGCCGACGACATGTTGGCCGAGCCGCGGGCACCCTATCCGCATGCCCGTGA
AGTGGCTATGTTGCGCGCGTATCAGGCACGCTGCGCAGCATTGATCGGCGGCTACACCCGGACGGCAGAAGCCCACGCGT
TGGTGCGCAGCGGATGGGCCCACGATGTGGTTGCCGCACGTTCTGTGGTTCGTGCTCGTGCTCCGCGGGTAGTGGCTCTC
GACGACAGCGGATACGCCGAGGAAAGTGACCCGGCAGCCCGCACTGCGCGACTTCCGTCGATTGCACTGCGCGCGGCCCG
CTCGGCGCTTGCGGCTGGAGCACCGGTGCTGGTGCAGGTGCCGCGGCGTGGGTATGTGCCATCGTTGGCCTGCGGGCGTT
GCCGTACATTAGCCCGTTGTCGACACTGCACGGGTCCGCTTTCGCTGCTAGACCGTGCCACCCCCGGTACCGTCTGTTGC
TGGTGCGGCCGAGCCGATCTGACGTTACGTTGCGCGCGCTGCGGGTCGGAGGTGGTGCGCGCGGTGGTGGTTGGGGCTCG
CCGCACGGCCGAAGAGCTCGGCCGTGCGTTCGCCGGTATGCCGGTCATCACCTCGGTGGGCGACACCATCGTGCCGGAAG
TCGGTGCCCGACCGGCTCTGGTGGTCGCCACCCCCGGGGCTGAACCGCGTGCTCCAGGTGGGTACGGGGCAGCGCTGCTG
CTGGATACCTGGGCATTGCTAGGCCGGCAAGATCTGCGAGCGGCCGAGGAAGCCCTGTGGCGTTGGATGACCGCGGCCGC
ATTAGTGCGTGCGCGGGGTGATGGGGGGGTGGTGATGGTAGTCGCTGAAGCATCTATTCCGACAGTGCAGTCCTTGATGC
GCTGGGATCCGGCCAGTCACGCGGAGGCTGAACTGGCGGCTAGAACCGAAGTTGGCTTGCCACCTAGCGTGCACATCGCG
GCAGTCGATGGTACCACCGGCGCAGTTAACGAGTTGTTGCAGGAGGCCCGGTTGCCCGATGAAGCCGATTTGCTCGGCCC
GGTGGACCTGCCGCAGGGTGTGCGCCGCCCAGCAGGCACCCCCCTCGGCGCACCGATCAGCAGGCTGTTGGTACGGGTGC
CTCGTGAGCAGGGCTGGCAACTGGCAGCGAGTCTGCGGCGTGGCATCGGCGTGCTGAGCGTGCGGCAAACTCATCAACTG
GTCCGTGTGCAGATTGACCCGCTGCACATCGGATAG

Upstream 100 bases:

>100_bases
ATCGAGACAACCGTTGTGGCTGGTCGGTTGGCTCGACGTGATCCGGGCCAGTGGTGCCCGCACGCCCGTTGCGGTGGAGC
CCATCGCGCGAGTGTTGCCG

Downstream 100 bases:

>100_bases
CCTCACAGCTTGCGGGCGACGAAAATCAAGTATTGTCACTCTAACTTATTGCTCCCCTACTTCAGACGTTGCCGGCTGAG
CTCGGCGAGTTTGGCATCGA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFVLERRNDTEHFGKLGWLDRVVSAQPVLTAEVRRLVDAVV
ARYAGTRPDVLRLAVPTRHARVERETLAIPVSPLPLIPGPVDPSGWEVYGRGGQFLTALAESRAARAVWQALPGEQWADR
FAEAAAQAIRAGRAALAIVPDQRDLDVLWRAVTTRVDERSVVALSAGLGQAVRYQRWLKVLRGSARLVIGTRSAVFAPVN
DLGLVMVWSDADDMLAEPRAPYPHAREVAMLRAYQARCAALIGGYTRTAEAHALVRSGWAHDVVAARSVVRARAPRVVAL
DDSGYAEESDPAARTARLPSIALRAARSALAAGAPVLVQVPRRGYVPSLACGRCRTLARCRHCTGPLSLLDRATPGTVCC
WCGRADLTLRCARCGSEVVRAVVVGARRTAEELGRAFAGMPVITSVGDTIVPEVGARPALVVATPGAEPRAPGGYGAALL
LDTWALLGRQDLRAAEEALWRWMTAAALVRARGDGGVVMVVAEASIPTVQSLMRWDPASHAEAELAARTEVGLPPSVHIA
AVDGTTGAVNELLQEARLPDEADLLGPVDLPQGVRRPAGTPLGAPISRLLVRVPREQGWQLAASLRRGIGVLSVRQTHQL
VRVQIDPLHIG

Sequences:

>Translated_651_residues
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFVLERRNDTEHFGKLGWLDRVVSAQPVLTAEVRRLVDAVV
ARYAGTRPDVLRLAVPTRHARVERETLAIPVSPLPLIPGPVDPSGWEVYGRGGQFLTALAESRAARAVWQALPGEQWADR
FAEAAAQAIRAGRAALAIVPDQRDLDVLWRAVTTRVDERSVVALSAGLGQAVRYQRWLKVLRGSARLVIGTRSAVFAPVN
DLGLVMVWSDADDMLAEPRAPYPHAREVAMLRAYQARCAALIGGYTRTAEAHALVRSGWAHDVVAARSVVRARAPRVVAL
DDSGYAEESDPAARTARLPSIALRAARSALAAGAPVLVQVPRRGYVPSLACGRCRTLARCRHCTGPLSLLDRATPGTVCC
WCGRADLTLRCARCGSEVVRAVVVGARRTAEELGRAFAGMPVITSVGDTIVPEVGARPALVVATPGAEPRAPGGYGAALL
LDTWALLGRQDLRAAEEALWRWMTAAALVRARGDGGVVMVVAEASIPTVQSLMRWDPASHAEAELAARTEVGLPPSVHIA
AVDGTTGAVNELLQEARLPDEADLLGPVDLPQGVRRPAGTPLGAPISRLLVRVPREQGWQLAASLRRGIGVLSVRQTHQL
VRVQIDPLHIG
>Mature_651_residues
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFVLERRNDTEHFGKLGWLDRVVSAQPVLTAEVRRLVDAVV
ARYAGTRPDVLRLAVPTRHARVERETLAIPVSPLPLIPGPVDPSGWEVYGRGGQFLTALAESRAARAVWQALPGEQWADR
FAEAAAQAIRAGRAALAIVPDQRDLDVLWRAVTTRVDERSVVALSAGLGQAVRYQRWLKVLRGSARLVIGTRSAVFAPVN
DLGLVMVWSDADDMLAEPRAPYPHAREVAMLRAYQARCAALIGGYTRTAEAHALVRSGWAHDVVAARSVVRARAPRVVAL
DDSGYAEESDPAARTARLPSIALRAARSALAAGAPVLVQVPRRGYVPSLACGRCRTLARCRHCTGPLSLLDRATPGTVCC
WCGRADLTLRCARCGSEVVRAVVVGARRTAEELGRAFAGMPVITSVGDTIVPEVGARPALVVATPGAEPRAPGGYGAALL
LDTWALLGRQDLRAAEEALWRWMTAAALVRARGDGGVVMVVAEASIPTVQSLMRWDPASHAEAELAARTEVGLPPSVHIA
AVDGTTGAVNELLQEARLPDEADLLGPVDLPQGVRRPAGTPLGAPISRLLVRVPREQGWQLAASLRRGIGVLSVRQTHQL
VRVQIDPLHIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily

Homologues:

Organism=Escherichia coli, GI1790370, Length=449, Percent_Identity=27.8396436525612, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_MYCLE (Q9CCQ3)

Other databases:

- EMBL:   AL583918
- PIR:   D86977
- RefSeq:   NP_301464.1
- ProteinModelPortal:   Q9CCQ3
- SMR:   Q9CCQ3
- EnsemblBacteria:   EBMYCT00000029344
- GeneID:   909319
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0548
- NMPDR:   fig|272631.1.peg.336
- Leproma:   ML0548
- GeneTree:   EBGT00050000017134
- HOGENOM:   HBG350750
- OMA:   QALVRWD
- ProtClustDB:   PRK14873
- BioCyc:   MLEP272631:ML0548-MONOMER
- InterPro:   IPR005259
- TIGRFAMs:   TIGR00595

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 69924; Mature: 69924

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFVLERRNDTEHFGKLGWLDR
CCCCCCCCCCHHHHEECCCCCCCCCCCEEEEEECCEEECCEEEECCCCHHHHHHHHHHHH
VVSAQPVLTAEVRRLVDAVVARYAGTRPDVLRLAVPTRHARVERETLAIPVSPLPLIPGP
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHHEEECCCCCCCCCCC
VDPSGWEVYGRGGQFLTALAESRAARAVWQALPGEQWADRFAEAAAQAIRAGRAALAIVP
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECC
DQRDLDVLWRAVTTRVDERSVVALSAGLGQAVRYQRWLKVLRGSARLVIGTRSAVFAPVN
CCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCEEEECCC
DLGLVMVWSDADDMLAEPRAPYPHAREVAMLRAYQARCAALIGGYTRTAEAHALVRSGWA
CCEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
HDVVAARSVVRARAPRVVALDDSGYAEESDPAARTARLPSIALRAARSALAAGAPVLVQV
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
PRRGYVPSLACGRCRTLARCRHCTGPLSLLDRATPGTVCCWCGRADLTLRCARCGSEVVR
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AVVVGARRTAEELGRAFAGMPVITSVGDTIVPEVGARPALVVATPGAEPRAPGGYGAALL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
LDTWALLGRQDLRAAEEALWRWMTAAALVRARGDGGVVMVVAEASIPTVQSLMRWDPASH
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCC
AEAELAARTEVGLPPSVHIAAVDGTTGAVNELLQEARLPDEADLLGPVDLPQGVRRPAGT
CHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCC
PLGAPISRLLVRVPREQGWQLAASLRRGIGVLSVRQTHQLVRVQIDPLHIG
CCCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEEEHHCCEEEEEEEECEECC
>Mature Secondary Structure
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFVLERRNDTEHFGKLGWLDR
CCCCCCCCCCHHHHEECCCCCCCCCCCEEEEEECCEEECCEEEECCCCHHHHHHHHHHHH
VVSAQPVLTAEVRRLVDAVVARYAGTRPDVLRLAVPTRHARVERETLAIPVSPLPLIPGP
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHHEEECCCCCCCCCCC
VDPSGWEVYGRGGQFLTALAESRAARAVWQALPGEQWADRFAEAAAQAIRAGRAALAIVP
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECC
DQRDLDVLWRAVTTRVDERSVVALSAGLGQAVRYQRWLKVLRGSARLVIGTRSAVFAPVN
CCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCEEEECCC
DLGLVMVWSDADDMLAEPRAPYPHAREVAMLRAYQARCAALIGGYTRTAEAHALVRSGWA
CCEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
HDVVAARSVVRARAPRVVALDDSGYAEESDPAARTARLPSIALRAARSALAAGAPVLVQV
HHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
PRRGYVPSLACGRCRTLARCRHCTGPLSLLDRATPGTVCCWCGRADLTLRCARCGSEVVR
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AVVVGARRTAEELGRAFAGMPVITSVGDTIVPEVGARPALVVATPGAEPRAPGGYGAALL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
LDTWALLGRQDLRAAEEALWRWMTAAALVRARGDGGVVMVVAEASIPTVQSLMRWDPASH
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCC
AEAELAARTEVGLPPSVHIAAVDGTTGAVNELLQEARLPDEADLLGPVDLPQGVRRPAGT
CHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCC
PLGAPISRLLVRVPREQGWQLAASLRRGIGVLSVRQTHQLVRVQIDPLHIG
CCCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEEEHHCCEEEEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002