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The map label for this gene is metK [H]

Identifier: 221229678

GI number: 221229678

Start: 659730

End: 660941

Strand: Direct

Name: metK [H]

Synonym: MLBr_00544

Alternate gene names: 221229678

Gene position: 659730-660941 (Clockwise)

Preceding gene: 221229677

Following gene: 221229679

Centisome position: 20.19

GC content: 61.22

Gene sequence:

>1212_bases
GTGAGTGAGAAGGGTCGGCTGTTCACTAGCGAGTCGGTGACTGAGGGACATCCCGACAAGATCTGTGATGCGATCAGCGA
CTCGATCCTTGACGCACTTTTGGCGGAGGATCCTTGCTCACGTGTCGCGGTCGAGACGTTGGTCACCACCGGGCAGGTGC
ATGTGGTGGGTGAAGTGACGACGTTGGCCAAGACGGCGTTCGCTGATATCAGTAATACGGTCCGCGAACGTATTCTCGAT
ATCGGCTACGACTCGTCGGACAAGGGCTTCGATGGGGCGTCGTGCGGAGTTAACATTGGCATCGGCGCTCAGTCGTCTGA
CATTGCTCAAGGCGTCAATACCGCCCATGAAGTACGCGTCGAGGGCGCGGCGGATCCGCTGGACGCCCAGGGTGCTGGTG
ACCAAGGCCTGATGTTCGGTTACGCGATCAATGACACCCCGGAACTGATGCCGCTACCGATTGCACTGGCCCACCGACTG
GCGCGAAGGCTGACCGAGGTACGCAAGAACGGCGTGCTGCCCTACCTGCGTTCCGACGGCAAGACCCAGGTCACTATCGC
CTACGAGGACAATGTCCCAGTGCGTTTGGACACTGTGGTCATCTCCACTCAGCACGCCGCTGGTGTCGACCTGGATGCCA
CGCTGGCTCCTGATATCCGGGAGAAGGTGCTCAACACCGTTATTGACGATCTGTCTCATGACACCTTGGATGTATCGTCG
GTGCGGGTGCTGGTAAACCCGACCGGCAAGTTCGTGCTAGGTGGGCCGATGGGCGATGCCGGGCTCACCGGTCGCAAGAT
CATCGTCGACACCTACGGTGGCTGGGCGCGTCACGGCGGCGGCGCCTTCTCTGGCAAGGATCCGTCCAAGGTGGACCGGT
CGGCAGCCTACGCGATGCGCTGGGTGGCCAAGAACATCGTCGCTGCCGGGCTGGCGGAGCGAATCGAGGTGCAGGTGGCA
TACGCCATCGGCAAAGCCGCCCCGGTCGGTTTGTTCGTCGAGACCTTTGGCACTGAGGCGGTCGATCCGGCCAAAATCGA
GAAAGCCATCGGCGAGGTGTTCGATCTGCGTCCCGGCGCGATCATCCGCGACCTGCATCTGCTGCGCCCAATTTACGCGC
AAACCGCTGCCTATGGGCACTTCGGTCGCACTGACGTCGAACTGCCATGGGAGCAGCTCAACAAAGTCGACGATCTCAAG
CGCGCCATCTAG

Upstream 100 bases:

>100_bases
TGTGCAAGTGTTGGGTGATCCCTTTTCGAGTGGATGTACTGAGTCTGATGCCAGCGAATATAAATTCGTTTAGCTAATTA
TGGAAAGGAAATAGTCAATA

Downstream 100 bases:

>100_bases
GGGCGCCGCATCGTTCACATGGCGGGTGGCATGGGGTGGTCGTGGTCAAGCGCGGGCAGGCCGTCGATGCTGAGTCGGTT
GACTTCGGCGAGGTGAGCTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEKGRLFTSESVTEGHPDKICDAISDSILDALLAEDPCSRVAVETLVTTGQVHVVGEVTTLAKTAFADISNTVRERILD
IGYDSSDKGFDGASCGVNIGIGAQSSDIAQGVNTAHEVRVEGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRL
ARRLTEVRKNGVLPYLRSDGKTQVTIAYEDNVPVRLDTVVISTQHAAGVDLDATLAPDIREKVLNTVIDDLSHDTLDVSS
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERIEVQVA
YAIGKAAPVGLFVETFGTEAVDPAKIEKAIGEVFDLRPGAIIRDLHLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDDLK
RAI

Sequences:

>Translated_403_residues
MSEKGRLFTSESVTEGHPDKICDAISDSILDALLAEDPCSRVAVETLVTTGQVHVVGEVTTLAKTAFADISNTVRERILD
IGYDSSDKGFDGASCGVNIGIGAQSSDIAQGVNTAHEVRVEGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRL
ARRLTEVRKNGVLPYLRSDGKTQVTIAYEDNVPVRLDTVVISTQHAAGVDLDATLAPDIREKVLNTVIDDLSHDTLDVSS
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERIEVQVA
YAIGKAAPVGLFVETFGTEAVDPAKIEKAIGEVFDLRPGAIIRDLHLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDDLK
RAI
>Mature_402_residues
SEKGRLFTSESVTEGHPDKICDAISDSILDALLAEDPCSRVAVETLVTTGQVHVVGEVTTLAKTAFADISNTVRERILDI
GYDSSDKGFDGASCGVNIGIGAQSSDIAQGVNTAHEVRVEGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRLA
RRLTEVRKNGVLPYLRSDGKTQVTIAYEDNVPVRLDTVVISTQHAAGVDLDATLAPDIREKVLNTVIDDLSHDTLDVSSV
RVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERIEVQVAY
AIGKAAPVGLFVETFGTEAVDPAKIEKAIGEVFDLRPGAIIRDLHLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDDLKR
AI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=400, Percent_Identity=52.25, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI5174529, Length=394, Percent_Identity=52.5380710659898, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1789311, Length=397, Percent_Identity=57.4307304785894, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17538494, Length=396, Percent_Identity=52.7777777777778, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=53, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17538498, Length=396, Percent_Identity=52.2727272727273, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551082, Length=396, Percent_Identity=50.5050505050505, Blast_Score=357, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI32565905, Length=396, Percent_Identity=47.2222222222222, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=51.4367816091954, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=50.8720930232558, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32565903, Length=315, Percent_Identity=50.7936507936508, Blast_Score=284, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI32565909, Length=92, Percent_Identity=52.1739130434783, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=401, Percent_Identity=53.1172069825436, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323209, Length=393, Percent_Identity=54.4529262086514, Blast_Score=378, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=51.2820512820513, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=51.2820512820513, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=51.2820512820513, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=51.2820512820513, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=51.2820512820513, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=51.2820512820513, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580527, Length=390, Percent_Identity=50.5128205128205, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580525, Length=390, Percent_Identity=50.5128205128205, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=49.2063492063492, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=49.5798319327731, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42921; Mature: 42790

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKGRLFTSESVTEGHPDKICDAISDSILDALLAEDPCSRVAVETLVTTGQVHVVGEVT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEHHH
TLAKTAFADISNTVRERILDIGYDSSDKGFDGASCGVNIGIGAQSSDIAQGVNTAHEVRV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCHHEEEE
EGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRLARRLTEVRKNGVLPYLRSDG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCC
KTQVTIAYEDNVPVRLDTVVISTQHAAGVDLDATLAPDIREKVLNTVIDDLSHDTLDVSS
CEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR
EEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHH
WVAKNIVAAGLAERIEVQVAYAIGKAAPVGLFVETFGTEAVDPAKIEKAIGEVFDLRPGA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCH
IIRDLHLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDDLKRAI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKGRLFTSESVTEGHPDKICDAISDSILDALLAEDPCSRVAVETLVTTGQVHVVGEVT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEHHH
TLAKTAFADISNTVRERILDIGYDSSDKGFDGASCGVNIGIGAQSSDIAQGVNTAHEVRV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCHHEEEE
EGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRLARRLTEVRKNGVLPYLRSDG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCC
KTQVTIAYEDNVPVRLDTVVISTQHAAGVDLDATLAPDIREKVLNTVIDDLSHDTLDVSS
CEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR
EEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHH
WVAKNIVAAGLAERIEVQVAYAIGKAAPVGLFVETFGTEAVDPAKIEKAIGEVFDLRPGA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCH
IIRDLHLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDDLKRAI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA