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The map label for this gene is dfp [H]

Identifier: 221229677

GI number: 221229677

Start: 658342

End: 659601

Strand: Direct

Name: dfp [H]

Synonym: MLBr_00543

Alternate gene names: 221229677

Gene position: 658342-659601 (Clockwise)

Preceding gene: 221229676

Following gene: 221229678

Centisome position: 20.14

GC content: 63.25

Gene sequence:

>1260_bases
ATGGACTGCAAGCGGGTCGTTGTCGGCGTCTCCGGGGGTATCGCCGCGTATAAGGCGTGTACCGTGGTTCGTCAGCTCAT
TGACGCCGGTCACGAGGTGCGGGTCATCCCAACTGAGTCGGCGTTGCGCTTCGTCGGAACTGCCACCTTCGAGGCACTCT
CCGGCCAACCGGTGTACACCGACGTGTTCGACGATATTCCGGCAGTGTCGCATGTGTACCTCGGCAAGCAGGCCGACTTG
GTGGTAGTGGCGCCGGCCACCGCCGACCTATTGGCCCGCGCAGTGCACGGCCGAGCCGACGATCTGTTAACCGCGACCCT
GCTCACCGCACGGTGTCCGATCATGTTCGCGCCGGCGATGCACACCGAGATGTGGTTGCATCCGGCTACCGTCGACAACG
TGGCCACGTTGCGTCGCCGCGGCGCGATTGTTCTGGAACCCGCGTCCGGGCGCCTCACCGGTACCGATAGCGGAGCGGGA
CGCCTGCCCGAGGCGGAAGAGATCACAACCCTTGCCCAGTTACTGCTGGAGCGGCACGACGCTTTACCCTATGACCTAAC
GGGCAGAAAACTTCTGGTGACCTCCGGTGGTACCCGCGAGTCGATCGATCCGGTGCGCTTCATTGGGAACCGCAGCTCCG
GTAAGCAGGGCTATGCTGTCGCGCGGGTCGCTGTTCAGCGCGGCGCCGAAGTCACGTTGATCGCTGGGCACACCGCGGGG
CTGACCAACCCTGCTGGGGTCGATGTGGTGCATGTTGGCTCGGCGCAGCAACTCGGAGACGCCGTTTCCAAGCACGCTCC
CGACTTTGACGTGTTGGTGATGGCAGCGGCCGTGGCTGATTTCCGGCCCGCCCAAGTTGCTACCGCCAAGATCAAAAAAG
GCCCCGATGACCAGGACGAGCCGCTGATCGAGTTGGTGTACAACGAAGACGTGTTGGCTGCCGCGGTGCGGGCCCGTACG
CATGGGCAGTTGCCCAACATGCGGGCCATTGTGGGATTCGCCGCAGAAACCGGGGATGCCAGCGGCGATGTGTTGTTTCA
TGCTCGCGCTAAGCTGCGGCGCAAGGGCTGTGACCTTTTAGTCGTGAATGCTGTGGGTGACGGCAGGGCCTTTGAGGTGG
ACAACAACGACGGCTGGCTGCTGGCGTCCGACGGTACCGAGTTGGCGCTACAGCACGGCTCCAAGATGCTCATGGCCAGT
CGTATCGTTGATGGGATTGTCACGTTTCTGCATGGCCTTCCCGATCTGCGAGGCGGGTGA

Upstream 100 bases:

>100_bases
CTGGGCTGCAGGAGAAACCGTTGTCCATCGCGATGCGCGAGATTCACGCCGATCTGCTCGAGCACACCGAGGGCGAGTAA
ACAGGGCAGGGCCGGAGGTG

Downstream 100 bases:

>100_bases
CTGACTGGCTCGTGTTCTGTAAGGCTCCTGTGCAAGTGTTGGGTGATCCCTTTTCGAGTGGATGTACTGAGTCTGATGCC
AGCGAATATAAATTCGTTTA

Product: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDCKRVVVGVSGGIAAYKACTVVRQLIDAGHEVRVIPTESALRFVGTATFEALSGQPVYTDVFDDIPAVSHVYLGKQADL
VVVAPATADLLARAVHGRADDLLTATLLTARCPIMFAPAMHTEMWLHPATVDNVATLRRRGAIVLEPASGRLTGTDSGAG
RLPEAEEITTLAQLLLERHDALPYDLTGRKLLVTSGGTRESIDPVRFIGNRSSGKQGYAVARVAVQRGAEVTLIAGHTAG
LTNPAGVDVVHVGSAQQLGDAVSKHAPDFDVLVMAAAVADFRPAQVATAKIKKGPDDQDEPLIELVYNEDVLAAAVRART
HGQLPNMRAIVGFAAETGDASGDVLFHARAKLRRKGCDLLVVNAVGDGRAFEVDNNDGWLLASDGTELALQHGSKMLMAS
RIVDGIVTFLHGLPDLRGG

Sequences:

>Translated_419_residues
MDCKRVVVGVSGGIAAYKACTVVRQLIDAGHEVRVIPTESALRFVGTATFEALSGQPVYTDVFDDIPAVSHVYLGKQADL
VVVAPATADLLARAVHGRADDLLTATLLTARCPIMFAPAMHTEMWLHPATVDNVATLRRRGAIVLEPASGRLTGTDSGAG
RLPEAEEITTLAQLLLERHDALPYDLTGRKLLVTSGGTRESIDPVRFIGNRSSGKQGYAVARVAVQRGAEVTLIAGHTAG
LTNPAGVDVVHVGSAQQLGDAVSKHAPDFDVLVMAAAVADFRPAQVATAKIKKGPDDQDEPLIELVYNEDVLAAAVRART
HGQLPNMRAIVGFAAETGDASGDVLFHARAKLRRKGCDLLVVNAVGDGRAFEVDNNDGWLLASDGTELALQHGSKMLMAS
RIVDGIVTFLHGLPDLRGG
>Mature_419_residues
MDCKRVVVGVSGGIAAYKACTVVRQLIDAGHEVRVIPTESALRFVGTATFEALSGQPVYTDVFDDIPAVSHVYLGKQADL
VVVAPATADLLARAVHGRADDLLTATLLTARCPIMFAPAMHTEMWLHPATVDNVATLRRRGAIVLEPASGRLTGTDSGAG
RLPEAEEITTLAQLLLERHDALPYDLTGRKLLVTSGGTRESIDPVRFIGNRSSGKQGYAVARVAVQRGAEVTLIAGHTAG
LTNPAGVDVVHVGSAQQLGDAVSKHAPDFDVLVMAAAVADFRPAQVATAKIKKGPDDQDEPLIELVYNEDVLAAAVRART
HGQLPNMRAIVGFAAETGDASGDVLFHARAKLRRKGCDLLVVNAVGDGRAFEVDNNDGWLLASDGTELALQHGSKMLMAS
RIVDGIVTFLHGLPDLRGG

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine [H]

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family [H]

Homologues:

Organism=Homo sapiens, GI71725351, Length=182, Percent_Identity=31.8681318681319, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87082301, Length=408, Percent_Identity=39.7058823529412, Blast_Score=228, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24657262, Length=177, Percent_Identity=28.2485875706215, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382 [H]

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein [H]

EC number: =4.1.1.36; =6.3.2.5 [H]

Molecular weight: Translated: 44213; Mature: 44213

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCKRVVVGVSGGIAAYKACTVVRQLIDAGHEVRVIPTESALRFVGTATFEALSGQPVYT
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEH
DVFDDIPAVSHVYLGKQADLVVVAPATADLLARAVHGRADDLLTATLLTARCPIMFAPAM
HHHHCCCHHHHEEECCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCC
HTEMWLHPATVDNVATLRRRGAIVLEPASGRLTGTDSGAGRLPEAEEITTLAQLLLERHD
CHHEEECCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
ALPYDLTGRKLLVTSGGTRESIDPVRFIGNRSSGKQGYAVARVAVQRGAEVTLIAGHTAG
CCCCCCCCCEEEEECCCCCCCCCHHHEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCC
LTNPAGVDVVHVGSAQQLGDAVSKHAPDFDVLVMAAAVADFRPAQVATAKIKKGPDDQDE
CCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
PLIELVYNEDVLAAAVRARTHGQLPNMRAIVGFAAETGDASGDVLFHARAKLRRKGCDLL
CEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHCCCCEE
VVNAVGDGRAFEVDNNDGWLLASDGTELALQHGSKMLMASRIVDGIVTFLHGLPDLRGG
EEEECCCCEEEEEECCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDCKRVVVGVSGGIAAYKACTVVRQLIDAGHEVRVIPTESALRFVGTATFEALSGQPVYT
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEH
DVFDDIPAVSHVYLGKQADLVVVAPATADLLARAVHGRADDLLTATLLTARCPIMFAPAM
HHHHCCCHHHHEEECCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCC
HTEMWLHPATVDNVATLRRRGAIVLEPASGRLTGTDSGAGRLPEAEEITTLAQLLLERHD
CHHEEECCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
ALPYDLTGRKLLVTSGGTRESIDPVRFIGNRSSGKQGYAVARVAVQRGAEVTLIAGHTAG
CCCCCCCCCEEEEECCCCCCCCCHHHEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCC
LTNPAGVDVVHVGSAQQLGDAVSKHAPDFDVLVMAAAVADFRPAQVATAKIKKGPDDQDE
CCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
PLIELVYNEDVLAAAVRARTHGQLPNMRAIVGFAAETGDASGDVLFHARAKLRRKGCDLL
CEEEEEECCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHCCCCEE
VVNAVGDGRAFEVDNNDGWLLASDGTELALQHGSKMLMASRIVDGIVTFLHGLPDLRGG
EEEECCCCEEEEEECCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]