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The map label for this gene is cysS

Identifier: 221229565

GI number: 221229565

Start: 412132

End: 413553

Strand: Direct

Name: cysS

Synonym: MLBr_00323

Alternate gene names: 221229565

Gene position: 412132-413553 (Clockwise)

Preceding gene: 221229564

Following gene: 221229566

Centisome position: 12.61

GC content: 60.34

Gene sequence:

>1422_bases
GTGATCGATAGCGGCCATTTGCGGCTCCACGATACGGTAGCTGGTGCTGTGCGCGATTTCGTTCCGCTGCGAGCCGGGCA
CGTCTCGATCTACCTGTGTGGTGCCACCGTTCAAGGACAGCCGCACATCGGGCACGTCCGCAGCGGGGTAGCCTTCGACA
TTCTGCGCCGATGGCTCATGGCACGCGGCTATGACGTCGCGTTCATCCGCAACGTCACCGATATCGATGACAAGATTCTT
AACAAGGCTGCCGCGGCTGGCCGGCCGTGGTGGGAATGGGCGGCCACCCACGAGCGCGCGTTTACCGCGGCCTATGACGC
CTTGGACGTTTTGCCGCCGTCAGCAGAGCCGCGAGCCACCGGGCACATTACCCAGATGATCGAGCTGATCGAGCTGTTGA
TCGAGACAGGTCACGCCTATACGGGCGGCTCTGATGTTTATTTCGACGTGCTCAGCTACCCGGATTATGGCCAGCTATCT
GGGCACAAGATGGACTATATCCACCAAGGCGAAGGTGTGACTACCGGCAAACGTGATCAACGCGACTTTACTTTGTGGAA
GGGCGCCAAGTCCGGTGAACCGTCGTGGCCTACGCCGTGGGGCCGTGGCCGTCCAGGCTGGCACCTGGAATGCTCGGCAA
TGGCTCGTGCTTATCTTGGTTCTGAATTTGACATCCATTGCGGTGGAATGGATTTAGTTTTTCCGCACCACGAAAATGAG
ATCGCGCAGAGCCGCGCTGTCGGGGATGGCTTCGCCCGTTACTGGCTGCACAACGGCTGGGTAACGATGGGTGGGGAGAA
GATGAGTAAGTCGCTGGGTAACGTGTTGTCCATCCCAGCCGTGCTGCAGCGGGTTCGGCCAGCCGAACTGCGTTACTATC
TCGGTAGCGCCCACTACCGGTCGATGCTGGAGTTCTCCGAGGCCGCGCTACAGGATGCGGTCAAAGCTTATGTCGGAGTG
GAGAATTTCCTGACTAGGGTGCGTACTCGGGTTGGTGCCGTTGGCACCGGCGAACTGACCCCACGGTTCGCTGCAGCACT
CGACGACGATCTGGCGGTGCCGATCGCGCTGGCTGAGGTGCACCACGCTCGTGTGGAGGGTAACCGAGCGCTCGACATCG
GCGACCACGAAGGTGCGTTGACGAACGCCGGCGCTATTCGAGCGATGATGGGCATCTTAGGTTGCGACCCGCTTGACGAA
CGTTGGGAATCCCGCGATGAGACGTCGGCGGCGCTAGCGGCTGTCGATGTGCTGGTGGCCGCTGAGCTAGAAAGCCGGCA
GATGGCACGCGAGCAGCGTAACTGGGTACTTGCCGATCAGATCCGCGATCGACTCAAGGATGCCGGTATTGAGGTAACTG
ATACCGTCAACGGGCCGCAGTGGGAACTGTTGGCTGGCGATAAGCAAGTTGATGCCCGGTAA

Upstream 100 bases:

>100_bases
CGAGGGCTTGTCCGCCATCGCGACTGCGTTGGTGGTCCAGGTCTGACAGCCGTGTGCTGAGAATCCGCCAACACGGAACC
AACAGGTAAGCTGGCCCGTC

Downstream 100 bases:

>100_bases
CTCGCGTCGCCGGGGGGCTGTCCGCAAATCCGGCACCAATAAGGGGACCACCGTTGGCTCGGGCGGCCAGCGTCGCCGTG
CGTTGGAGGGGCGTGGTCCG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIDSGHLRLHDTVAGAVRDFVPLRAGHVSIYLCGATVQGQPHIGHVRSGVAFDILRRWLMARGYDVAFIRNVTDIDDKIL
NKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIELIELLIETGHAYTGGSDVYFDVLSYPDYGQLS
GHKMDYIHQGEGVTTGKRDQRDFTLWKGAKSGEPSWPTPWGRGRPGWHLECSAMARAYLGSEFDIHCGGMDLVFPHHENE
IAQSRAVGDGFARYWLHNGWVTMGGEKMSKSLGNVLSIPAVLQRVRPAELRYYLGSAHYRSMLEFSEAALQDAVKAYVGV
ENFLTRVRTRVGAVGTGELTPRFAAALDDDLAVPIALAEVHHARVEGNRALDIGDHEGALTNAGAIRAMMGILGCDPLDE
RWESRDETSAALAAVDVLVAAELESRQMAREQRNWVLADQIRDRLKDAGIEVTDTVNGPQWELLAGDKQVDAR

Sequences:

>Translated_473_residues
MIDSGHLRLHDTVAGAVRDFVPLRAGHVSIYLCGATVQGQPHIGHVRSGVAFDILRRWLMARGYDVAFIRNVTDIDDKIL
NKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIELIELLIETGHAYTGGSDVYFDVLSYPDYGQLS
GHKMDYIHQGEGVTTGKRDQRDFTLWKGAKSGEPSWPTPWGRGRPGWHLECSAMARAYLGSEFDIHCGGMDLVFPHHENE
IAQSRAVGDGFARYWLHNGWVTMGGEKMSKSLGNVLSIPAVLQRVRPAELRYYLGSAHYRSMLEFSEAALQDAVKAYVGV
ENFLTRVRTRVGAVGTGELTPRFAAALDDDLAVPIALAEVHHARVEGNRALDIGDHEGALTNAGAIRAMMGILGCDPLDE
RWESRDETSAALAAVDVLVAAELESRQMAREQRNWVLADQIRDRLKDAGIEVTDTVNGPQWELLAGDKQVDAR
>Mature_473_residues
MIDSGHLRLHDTVAGAVRDFVPLRAGHVSIYLCGATVQGQPHIGHVRSGVAFDILRRWLMARGYDVAFIRNVTDIDDKIL
NKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIELIELLIETGHAYTGGSDVYFDVLSYPDYGQLS
GHKMDYIHQGEGVTTGKRDQRDFTLWKGAKSGEPSWPTPWGRGRPGWHLECSAMARAYLGSEFDIHCGGMDLVFPHHENE
IAQSRAVGDGFARYWLHNGWVTMGGEKMSKSLGNVLSIPAVLQRVRPAELRYYLGSAHYRSMLEFSEAALQDAVKAYVGV
ENFLTRVRTRVGAVGTGELTPRFAAALDDDLAVPIALAEVHHARVEGNRALDIGDHEGALTNAGAIRAMMGILGCDPLDE
RWESRDETSAALAAVDVLVAAELESRQMAREQRNWVLADQIRDRLKDAGIEVTDTVNGPQWELLAGDKQVDAR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=506, Percent_Identity=34.9802371541502, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI62240992, Length=296, Percent_Identity=35.472972972973, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI21269877, Length=296, Percent_Identity=35.472972972973, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI10835051, Length=296, Percent_Identity=35.472972972973, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI303304987, Length=296, Percent_Identity=35.472972972973, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1786737, Length=460, Percent_Identity=41.9565217391304, Blast_Score=325, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17509797, Length=433, Percent_Identity=29.0993071593533, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17509795, Length=433, Percent_Identity=29.0993071593533, Blast_Score=172, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6324082, Length=342, Percent_Identity=33.9181286549708, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19922218, Length=508, Percent_Identity=32.4803149606299, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI19922364, Length=265, Percent_Identity=36.2264150943396, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_MYCLB (B8ZUA9)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502981.1
- ProteinModelPortal:   B8ZUA9
- SMR:   B8ZUA9
- EnsemblBacteria:   EBMYCT00000087689
- GeneID:   7326379
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016491
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52125; Mature: 52125

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSGHLRLHDTVAGAVRDFVPLRAGHVSIYLCGATVQGQPHIGHVRSGVAFDILRRWLM
CCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCCHHHCCHHHHHHHHHHH
ARGYDVAFIRNVTDIDDKILNKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRAT
HCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GHITQMIELIELLIETGHAYTGGSDVYFDVLSYPDYGQLSGHKMDYIHQGEGVTTGKRDQ
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCC
RDFTLWKGAKSGEPSWPTPWGRGRPGWHLECSAMARAYLGSEFDIHCGGMDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCEEEECCCCCHH
IAQSRAVGDGFARYWLHNGWVTMGGEKMSKSLGNVLSIPAVLQRVRPAELRYYLGSAHYR
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SMLEFSEAALQDAVKAYVGVENFLTRVRTRVGAVGTGELTPRFAAALDDDLAVPIALAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHH
HHARVEGNRALDIGDHEGALTNAGAIRAMMGILGCDPLDERWESRDETSAALAAVDVLVA
HHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHH
AELESRQMAREQRNWVLADQIRDRLKDAGIEVTDTVNGPQWELLAGDKQVDAR
HHHHHHHHHHHHHCCEEHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIDSGHLRLHDTVAGAVRDFVPLRAGHVSIYLCGATVQGQPHIGHVRSGVAFDILRRWLM
CCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCCCHHHCCHHHHHHHHHHH
ARGYDVAFIRNVTDIDDKILNKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRAT
HCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
GHITQMIELIELLIETGHAYTGGSDVYFDVLSYPDYGQLSGHKMDYIHQGEGVTTGKRDQ
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCC
RDFTLWKGAKSGEPSWPTPWGRGRPGWHLECSAMARAYLGSEFDIHCGGMDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCEEEECCCCCHH
IAQSRAVGDGFARYWLHNGWVTMGGEKMSKSLGNVLSIPAVLQRVRPAELRYYLGSAHYR
HHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SMLEFSEAALQDAVKAYVGVENFLTRVRTRVGAVGTGELTPRFAAALDDDLAVPIALAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHHHH
HHARVEGNRALDIGDHEGALTNAGAIRAMMGILGCDPLDERWESRDETSAALAAVDVLVA
HHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHH
AELESRQMAREQRNWVLADQIRDRLKDAGIEVTDTVNGPQWELLAGDKQVDAR
HHHHHHHHHHHHHCCEEHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA