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The map label for this gene is ispF

Identifier: 221229564

GI number: 221229564

Start: 411601

End: 412077

Strand: Direct

Name: ispF

Synonym: MLBr_00322

Alternate gene names: 221229564

Gene position: 411601-412077 (Clockwise)

Preceding gene: 221229563

Following gene: 221229565

Centisome position: 12.59

GC content: 64.15

Gene sequence:

>477_bases
GTGAGTGAACTGCCCCGGGTCGGTTTGGGTTTCGATGTCCACGTGTTTGAACCGGGGCGGCCGTGTTGGCTGGTGGGGCT
GCTGTTCCCGGACAACGACGGCTGCGCCGGGCACTCCGATGGTGACGTAGCGGTGCATGCGCTGTGTGATGCCGTGTTGT
CGGCCGCAGGGCAGGGTGATATCGGCGGCCTGTTCGCCGTTGGCGATCCGCGTTGGGAACGCGTCAGCGGTGCTGACATG
CTGCGCCATGTAGTCGAATTGTTACTGCGACATGGCTACGAGGTCGTCAACGCGTCCGTTCAAGTGATCGGTAACCGACC
GAAAATCGGTCCGCGCCGTACCGAGGCGCAACGGTTGCTGTCAGCTCTGCTGCGAGCGCCAGTATCAGTGGCGGCGACGA
CCACCGAGGGACTTGGCCTGACTGGGCGTGGCGAGGGCTTGTCCGCCATCGCGACTGCGTTGGTGGTCCAGGTCTGA

Upstream 100 bases:

>100_bases
AACATCTCGGTGGCCAGGTTCAGGTGGTCGCCGGCGATCCACTGGCGTTCAAGATCACCACTCAGTTGGATCTGCTGCTG
GCGAAGAAGATTCTGCGCCG

Downstream 100 bases:

>100_bases
CAGCCGTGTGCTGAGAATCCGCCAACACGGAACCAACAGGTAAGCTGGCCCGTCGTGATCGATAGCGGCCATTTGCGGCT
CCACGATACGGTAGCTGGTG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDVAVHALCDAVLSAAGQGDIGGLFAVGDPRWERVSGADM
LRHVVELLLRHGYEVVNASVQVIGNRPKIGPRRTEAQRLLSALLRAPVSVAATTTEGLGLTGRGEGLSAIATALVVQV

Sequences:

>Translated_158_residues
MSELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDVAVHALCDAVLSAAGQGDIGGLFAVGDPRWERVSGADM
LRHVVELLLRHGYEVVNASVQVIGNRPKIGPRRTEAQRLLSALLRAPVSVAATTTEGLGLTGRGEGLSAIATALVVQV
>Mature_157_residues
SELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDVAVHALCDAVLSAAGQGDIGGLFAVGDPRWERVSGADML
RHVVELLLRHGYEVVNASVQVIGNRPKIGPRRTEAQRLLSALLRAPVSVAATTTEGLGLTGRGEGLSAIATALVVQV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=41.2903225806452, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_MYCLB (B8ZUA8)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502980.1
- ProteinModelPortal:   B8ZUA8
- SMR:   B8ZUA8
- EnsemblBacteria:   EBMYCT00000085172
- GeneID:   7326378
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016933
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16511; Mature: 16380

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDVAVHALCDAVLSAAGQGD
CCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
IGGLFAVGDPRWERVSGADMLRHVVELLLRHGYEVVNASVQVIGNRPKIGPRRTEAQRLL
CCCEEEECCCCHHHCCHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCHHHHHHHH
SALLRAPVSVAATTTEGLGLTGRGEGLSAIATALVVQV
HHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDVAVHALCDAVLSAAGQGD
CCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
IGGLFAVGDPRWERVSGADMLRHVVELLLRHGYEVVNASVQVIGNRPKIGPRRTEAQRLL
CCCEEEECCCCHHHCCHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCHHHHHHHH
SALLRAPVSVAATTTEGLGLTGRGEGLSAIATALVVQV
HHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA