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The map label for this gene is thiD

Identifier: 221229547

GI number: 221229547

Start: 379143

End: 379982

Strand: Direct

Name: thiD

Synonym: MLBr_00295

Alternate gene names: 221229547

Gene position: 379143-379982 (Clockwise)

Preceding gene: 221229546

Following gene: 221229551

Centisome position: 11.6

GC content: 62.74

Gene sequence:

>840_bases
GTGAACTACCTGCCAGCGCCGCCCCCGGGCACGACACCGCTACGGGTGCTGTCCATCGCCGGGTCGGACTCCGGGGGCGG
CGCGGGAATCCAGGCTGATATGCGCACCATGACCGTGCTGGGTGTGCATGCATGCACTGCGGTAACTGCGGTAACTATAC
AGAATACATTGGGAGTCGAAGGCTTTCACGAAATCCCCGCCGAAATCGTGGCCAGTCAGATCAAGGCTGTGGTCACCGAC
ATTGGCATCCAGTCGGCCAAGACCGGTATGCTGGCGTCGTCAAGCATCATCGCCGCGGTGGCAGAGACCTGGCTGCGGCT
CGAATTGACGACACCGCTGGTCGTCGACCCGGTGTGCGCATCTATGCACGGAGACCCACTGCTAACGGGTGAGGCCATGG
ATTCACTTCGCGATCGATTGTTTCCGCTTGCCACGGTAGTTACGCCGAATCTCGACGAAGTGCGTCTACTGGTGGGTATC
GACGTGGTCGACACCGAATCGCAACGTGCGGCAGCGATGGCCTTGCATGCCCTGGGCCCACAGTGGGCACTAGTCAAGGG
GGGACACCTGCGGTCATCGGACCGCAGCTGCGACCTGCTCTACGGTGGCTCTTCCGCAGGCGTTGCCTTCCACGAGTTCG
ATGCGCCGCGGGTGCAGACCGGCAACGACCACGGCGGTGGTGACACGTTGGCGGCTGCGGTGTCCTGCGCGCTAGCACAC
GGATACACTGTGCCGGACGCAGTTGGCTTCGGTAAGCGCTGGGTTACCGAATGCCTGCGCGACGCCTATCCGCTGGGCCG
TGGCCACGGCCCAGTCTCTCCATTATTTCAGAGGACATGA

Upstream 100 bases:

>100_bases
AGCACGGACTCGACACTGAAGAAGCCATCGAGATGGGGATGACGGAGAAATCAGCCGAGTTCGCCGAGCACGGCAATCGT
GTGTATCTGCCGCTCACGCA

Downstream 100 bases:

>100_bases
TAACTCGAGCAAATCACCGACAGCAGGAAGAAGGAACCGCCGCCCCTGATAGCATTGTGGTCCTCACCCACGCCGCCGGA
GAAATAGGGAATCATCACTG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYLPAPPPGTTPLRVLSIAGSDSGGGAGIQADMRTMTVLGVHACTAVTAVTIQNTLGVEGFHEIPAEIVASQIKAVVTD
IGIQSAKTGMLASSSIIAAVAETWLRLELTTPLVVDPVCASMHGDPLLTGEAMDSLRDRLFPLATVVTPNLDEVRLLVGI
DVVDTESQRAAAMALHALGPQWALVKGGHLRSSDRSCDLLYGGSSAGVAFHEFDAPRVQTGNDHGGGDTLAAAVSCALAH
GYTVPDAVGFGKRWVTECLRDAYPLGRGHGPVSPLFQRT

Sequences:

>Translated_279_residues
MNYLPAPPPGTTPLRVLSIAGSDSGGGAGIQADMRTMTVLGVHACTAVTAVTIQNTLGVEGFHEIPAEIVASQIKAVVTD
IGIQSAKTGMLASSSIIAAVAETWLRLELTTPLVVDPVCASMHGDPLLTGEAMDSLRDRLFPLATVVTPNLDEVRLLVGI
DVVDTESQRAAAMALHALGPQWALVKGGHLRSSDRSCDLLYGGSSAGVAFHEFDAPRVQTGNDHGGGDTLAAAVSCALAH
GYTVPDAVGFGKRWVTECLRDAYPLGRGHGPVSPLFQRT
>Mature_279_residues
MNYLPAPPPGTTPLRVLSIAGSDSGGGAGIQADMRTMTVLGVHACTAVTAVTIQNTLGVEGFHEIPAEIVASQIKAVVTD
IGIQSAKTGMLASSSIIAAVAETWLRLELTTPLVVDPVCASMHGDPLLTGEAMDSLRDRLFPLATVVTPNLDEVRLLVGI
DVVDTESQRAAAMALHALGPQWALVKGGHLRSSDRSCDLLYGGSSAGVAFHEFDAPRVQTGNDHGGGDTLAAAVSCALAH
GYTVPDAVGFGKRWVTECLRDAYPLGRGHGPVSPLFQRT

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=38.3720930232558, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325378, Length=250, Percent_Identity=36, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324997, Length=248, Percent_Identity=34.6774193548387, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324517, Length=253, Percent_Identity=32.8063241106719, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THID_MYCLE (Q9ZBL1)

Other databases:

- EMBL:   AL035159
- EMBL:   AL583918
- PIR:   T44742
- RefSeq:   NP_301332.1
- ProteinModelPortal:   Q9ZBL1
- SMR:   Q9ZBL1
- EnsemblBacteria:   EBMYCT00000029416
- GeneID:   908848
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0295
- NMPDR:   fig|272631.1.peg.204
- Leproma:   ML0295
- GeneTree:   EBGT00050000016703
- HOGENOM:   HBG476673
- OMA:   ACALAHG
- ProtClustDB:   PRK06427
- BioCyc:   MLEP272631:ML0295-MONOMER
- BRENDA:   2.7.4.7
- InterPro:   IPR013749
- InterPro:   IPR004399
- TIGRFAMs:   TIGR00097

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.49; =2.7.4.7

Molecular weight: Translated: 28983; Mature: 28983

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: BINDING 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLPAPPPGTTPLRVLSIAGSDSGGGAGIQADMRTMTVLGVHACTAVTAVTIQNTLGVE
CCCCCCCCCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFHEIPAEIVASQIKAVVTDIGIQSAKTGMLASSSIIAAVAETWLRLELTTPLVVDPVCA
CHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHEEEEECCCEEECHHHH
SMHGDPLLTGEAMDSLRDRLFPLATVVTPNLDEVRLLVGIDVVDTESQRAAAMALHALGP
HCCCCCEECHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEEEECCCCHHHHHHHHHHCCC
QWALVKGGHLRSSDRSCDLLYGGSSAGVAFHEFDAPRVQTGNDHGGGDTLAAAVSCALAH
CEEEEECCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GYTVPDAVGFGKRWVTECLRDAYPLGRGHGPVSPLFQRT
CCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNYLPAPPPGTTPLRVLSIAGSDSGGGAGIQADMRTMTVLGVHACTAVTAVTIQNTLGVE
CCCCCCCCCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFHEIPAEIVASQIKAVVTDIGIQSAKTGMLASSSIIAAVAETWLRLELTTPLVVDPVCA
CHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHEEEEECCCEEECHHHH
SMHGDPLLTGEAMDSLRDRLFPLATVVTPNLDEVRLLVGIDVVDTESQRAAAMALHALGP
HCCCCCEECHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEEEECCCCHHHHHHHHHHCCC
QWALVKGGHLRSSDRSCDLLYGGSSAGVAFHEFDAPRVQTGNDHGGGDTLAAAVSCALAH
CEEEEECCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GYTVPDAVGFGKRWVTECLRDAYPLGRGHGPVSPLFQRT
CCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002