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The map label for this gene is thiE

Identifier: 221229551

GI number: 221229551

Start: 382851

End: 383558

Strand: Direct

Name: thiE

Synonym: MLBr_00300

Alternate gene names: 221229551

Gene position: 382851-383558 (Clockwise)

Preceding gene: 221229547

Following gene: 221229552

Centisome position: 11.71

GC content: 65.82

Gene sequence:

>708_bases
GTGCAGGAGCCTCATAGGCAGCCCGCGATCTGTCTGTCCACCCGTCTCGCCAAGGCGCGGCTTTATCTATGCACCGACGC
GCGGCGTGAACGCGGCGACTTAGCCCAATTTGTCAACGCCGCCCTGGCCGGCGGGGTGGACATCGTCCAACTGCGAGACA
AGGGTTCGGTCGGTGAACAGCAGTTTGGCCCACTGGAGGCGCGCGATGCGTTGGCCGCGTGCGAGATCTTCACCGACGCG
ACGGGAAGACACGACGCCTTGTTCGCGGTCAACGACCGCGCCGATATTGCCCGCGCGGCGGGCGCCGACGTGCTGCATTT
GGGACAGGGCGATCTGCCGCCTGGTGTGGCGAGGCAGATCGTATGCCGCCAGATGCTGATCGGCCTGTCTACCCATGATC
GGCACCAGGTTGCCGCGGCTGTGGCCGCCTTAGACGCTGGCCTGGTCGACTATTTCTGTGTTGGGCCCTGTTGGCCTACC
CCTACCAAACCGGACCGCCCGGCTCCTGGTCTTGAGCTGGTGCGGGCTGCCGCTGAACTCGCTGGAGACAAACCGTGGTT
CGCTATCGGCGGTATCGACGCGCAGCGGCTGCCCGACGTGCTCCATGCCGGTGCCCGCCGGATTGTAGTGGTGCGGGCAA
TTACCGCGGCCGCCGACCCCCGCGCCGCAGCCGAACAACTCATTTCGACGCTGACAGCAACTAGCTGA

Upstream 100 bases:

>100_bases
TCAGTCACTCTGGCTGTGCCCACTCTCAGCCCCGCAGATTCGGACTGCCTGGGGACTCCCGTGTACTGAAGTTCTTCGAA
TCTTACGCTAGCGTCGCAGT

Downstream 100 bases:

>100_bases
TACGCTGACTCAGCTGCAGCGCTGCCAGAGCCGTATCACTTGGCATACACCACCCCGAAACTCCCATCTCGCATCTCGAT
CTCCATGATGCGAGGCAGAC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MQEPHRQPAICLSTRLAKARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQQFGPLEARDALAACEIFTDA
TGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQIVCRQMLIGLSTHDRHQVAAAVAALDAGLVDYFCVGPCWPT
PTKPDRPAPGLELVRAAAELAGDKPWFAIGGIDAQRLPDVLHAGARRIVVVRAITAAADPRAAAEQLISTLTATS

Sequences:

>Translated_235_residues
MQEPHRQPAICLSTRLAKARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQQFGPLEARDALAACEIFTDA
TGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQIVCRQMLIGLSTHDRHQVAAAVAALDAGLVDYFCVGPCWPT
PTKPDRPAPGLELVRAAAELAGDKPWFAIGGIDAQRLPDVLHAGARRIVVVRAITAAADPRAAAEQLISTLTATS
>Mature_235_residues
MQEPHRQPAICLSTRLAKARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQQFGPLEARDALAACEIFTDA
TGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQIVCRQMLIGLSTHDRHQVAAAVAALDAGLVDYFCVGPCWPT
PTKPDRPAPGLELVRAAAELAGDKPWFAIGGIDAQRLPDVLHAGARRIVVVRAITAAADPRAAAEQLISTLTATS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=198, Percent_Identity=36.3636363636364, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=233, Percent_Identity=29.1845493562232, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_MYCLB (B8ZU95)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502967.1
- ProteinModelPortal:   B8ZU95
- SMR:   B8ZU95
- EnsemblBacteria:   EBMYCT00000086227
- GeneID:   7326365
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015844
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 24847; Mature: 24847

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPHRQPAICLSTRLAKARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQ
CCCCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
QFGPLEARDALAACEIFTDATGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQI
HCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCEEECCCCCCCHHHHHHH
VCRQMLIGLSTHDRHQVAAAVAALDAGLVDYFCVGPCWPTPTKPDRPAPGLELVRAAAEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AGDKPWFAIGGIDAQRLPDVLHAGARRIVVVRAITAAADPRAAAEQLISTLTATS
CCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQEPHRQPAICLSTRLAKARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQ
CCCCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
QFGPLEARDALAACEIFTDATGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQI
HCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCEEECCCCCCCHHHHHHH
VCRQMLIGLSTHDRHQVAAAVAALDAGLVDYFCVGPCWPTPTKPDRPAPGLELVRAAAEL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AGDKPWFAIGGIDAQRLPDVLHAGARRIVVVRAITAAADPRAAAEQLISTLTATS
CCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA