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The map label for this gene is thiC [H]

Identifier: 221229546

GI number: 221229546

Start: 377503

End: 379146

Strand: Direct

Name: thiC [H]

Synonym: MLBr_00294

Alternate gene names: 221229546

Gene position: 377503-379146 (Clockwise)

Preceding gene: 221229542

Following gene: 221229547

Centisome position: 11.55

GC content: 59.31

Gene sequence:

>1644_bases
ATGACTGAAACCTTGTCGAAAACCACTGAACCATCCGTAACCACCGGACCGATCCCTGGAAGCAGCAAGGCTTACCGAGA
AGTAGCGAACCCGGATGGCGGACCTAGCCTGCGGGTACCGTTCCGCCGAGTACATCTATCCACCGGCGCCCATTTCGACC
TTTACGACACTTCCGGGCCCTACACCGATCCCGATGCGGTGATCAACCTGACGGCCGGGTTGCCGCCCAGGCCCGGCGTG
ATCCGTGACCGTGGCACACAACTGCAGCGGGCCCGTGCCGGAGAGATTACTGCGGAGATGGCGTTCATCGCTGACCGCGA
GGGCATGCCCGCCGAGTTGGTGCGCGTCGAGGTCGCCCTTGGCCGCGCGGTGATTCCTGCCAACCACAACCATCCTGAGA
TCGAGCCGATGATCATCGGTAAGGCATTTGCGGTCAAAGTCAACGCAAACATCGGCAATTCGGCGGTGACATCGTCGATC
GCCGAGGAGATCGACAAGATGGTGTGGGCCACTCGCTGGGGTGCAGACACAATCATGGACCTGTCCACCGGCAAGAACAT
TCACGAAACGCGTGAGTGGATCCTGCGCAATTCGCCGGTGCCGGTCGGCACTGTGCCGATCTATCAGGCGTTGGAAAAAG
TCAAGGGCGATCCAACCAAGCTAACTTGGGAGATCTACCGCGACACCGTGATCGAGCAGTGTGAACAGGGTGTGGATTAC
ATGACCGTGCACGCCGGTGTGCTACTGCGATATGTACTGTTGACCGCAAAGCGGGTCACCGGCATCGTGTCCCGCGGTGG
GTCAATCATGGCGTCCTGGTGTTTAGCCAATCATCGGGAGTCGTTCTTGTACACAAACTTTGCGGAGCTCTGCGATATCT
TCGCGCGCTACGACGTCACTTTCTCGCTTGGTGACGGACTGCGACCCGGCTCAATCGCCGACGCCAACGACACTGCACAG
TTCGCCGAGTTGCGCACTCTGGGGGAGCTATCAAAGATCGCGAAAGTCCATGGTGCACAGGTCATGATCGAGGGACCAGG
ACACATCCCGATGCATAAGATTGTGGAGAACGTGCGGCTAGAAGAGGAACTGTGCGAGGAGGCACCGTTTTACACGCTGG
GCCCGTTGGCCACCGATATCGCACCGGCCTATGATCACATCACGTCGGCGATTGGCGCGGCTGTTATCGCCCAAGCCGGT
ACCGCGATGCTGTGCTATGTAACCCCGAAAGAGCATCTGGGTCTGCCAGACCGCAAGGACGTCAAGGACGGCGTGATCGC
CTACAAGATCGCCGCGCATGCAGCGGACCTGGCCAAGGGCTATCCTCGCGCGCAGGAACGTGACGATGCACTGTCCACGG
CACGGTTCGAATTCCGCTGGAACGACCAATTCGCACTATCGCTTGATCCCCCCACCGCACGAGAGTTCCACGACGAGACG
TTGCCGGCCGAGCCGGCTAAGACGGCGCACTTCTGTTCGATGTGTGGGCCGAAATTCTGCTCTATGCGGATCACCGCTGA
TATACGAGTATATGCGGCCAAGCACGGACTCGACACTGAAGAAGCCATCGAGATGGGGATGACGGAGAAATCAGCCGAGT
TCGCCGAGCACGGCAATCGTGTGTATCTGCCGCTCACGCAGTGA

Upstream 100 bases:

>100_bases
AAAACCACGCGGGAGCACACACCAAGTGCGCTGAGAGGACGGATCGGGGCCGTCGACCGTATGAACCTGACCGGGTAATG
CCGGCGTAGGGAGATGAATA

Downstream 100 bases:

>100_bases
ACTACCTGCCAGCGCCGCCCCCGGGCACGACACCGCTACGGGTGCTGTCCATCGCCGGGTCGGACTCCGGGGGCGGCGCG
GGAATCCAGGCTGATATGCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTETLSKTTEPSVTTGPIPGSSKAYREVANPDGGPSLRVPFRRVHLSTGAHFDLYDTSGPYTDPDAVINLTAGLPPRPGV
IRDRGTQLQRARAGEITAEMAFIADREGMPAELVRVEVALGRAVIPANHNHPEIEPMIIGKAFAVKVNANIGNSAVTSSI
AEEIDKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTKLTWEIYRDTVIEQCEQGVDY
MTVHAGVLLRYVLLTAKRVTGIVSRGGSIMASWCLANHRESFLYTNFAELCDIFARYDVTFSLGDGLRPGSIADANDTAQ
FAELRTLGELSKIAKVHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAVIAQAG
TAMLCYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGYPRAQERDDALSTARFEFRWNDQFALSLDPPTAREFHDET
LPAEPAKTAHFCSMCGPKFCSMRITADIRVYAAKHGLDTEEAIEMGMTEKSAEFAEHGNRVYLPLTQ

Sequences:

>Translated_547_residues
MTETLSKTTEPSVTTGPIPGSSKAYREVANPDGGPSLRVPFRRVHLSTGAHFDLYDTSGPYTDPDAVINLTAGLPPRPGV
IRDRGTQLQRARAGEITAEMAFIADREGMPAELVRVEVALGRAVIPANHNHPEIEPMIIGKAFAVKVNANIGNSAVTSSI
AEEIDKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTKLTWEIYRDTVIEQCEQGVDY
MTVHAGVLLRYVLLTAKRVTGIVSRGGSIMASWCLANHRESFLYTNFAELCDIFARYDVTFSLGDGLRPGSIADANDTAQ
FAELRTLGELSKIAKVHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAVIAQAG
TAMLCYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGYPRAQERDDALSTARFEFRWNDQFALSLDPPTAREFHDET
LPAEPAKTAHFCSMCGPKFCSMRITADIRVYAAKHGLDTEEAIEMGMTEKSAEFAEHGNRVYLPLTQ
>Mature_546_residues
TETLSKTTEPSVTTGPIPGSSKAYREVANPDGGPSLRVPFRRVHLSTGAHFDLYDTSGPYTDPDAVINLTAGLPPRPGVI
RDRGTQLQRARAGEITAEMAFIADREGMPAELVRVEVALGRAVIPANHNHPEIEPMIIGKAFAVKVNANIGNSAVTSSIA
EEIDKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTKLTWEIYRDTVIEQCEQGVDYM
TVHAGVLLRYVLLTAKRVTGIVSRGGSIMASWCLANHRESFLYTNFAELCDIFARYDVTFSLGDGLRPGSIADANDTAQF
AELRTLGELSKIAKVHGAQVMIEGPGHIPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAVIAQAGT
AMLCYVTPKEHLGLPDRKDVKDGVIAYKIAAHAADLAKGYPRAQERDDALSTARFEFRWNDQFALSLDPPTAREFHDETL
PAEPAKTAHFCSMCGPKFCSMRITADIRVYAAKHGLDTEEAIEMGMTEKSAEFAEHGNRVYLPLTQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=593, Percent_Identity=58.5160202360877, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 59841; Mature: 59709

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLSKTTEPSVTTGPIPGSSKAYREVANPDGGPSLRVPFRRVHLSTGAHFDLYDTSGP
CCCCCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCEECCCEEEEECCCCEEEEECCCCC
YTDPDAVINLTAGLPPRPGVIRDRGTQLQRARAGEITAEMAFIADREGMPAELVRVEVAL
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCHHHHHHEEECCCCCCHHHHEEEHHH
GRAVIPANHNHPEIEPMIIGKAFAVKVNANIGNSAVTSSIAEEIDKMVWATRWGADTIMD
CCEEECCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTKLTWEIYRDTVIEQCEQGVDY
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCE
MTVHAGVLLRYVLLTAKRVTGIVSRGGSIMASWCLANHRESFLYTNFAELCDIFARYDVT
EEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCEE
FSLGDGLRPGSIADANDTAQFAELRTLGELSKIAKVHGAQVMIEGPGHIPMHKIVENVRL
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
EEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAVIAQAGTAMLCYVTPKEHLGLPDRKD
HHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCC
VKDGVIAYKIAAHAADLAKGYPRAQERDDALSTARFEFRWNDQFALSLDPPTAREFHDET
CCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECCEEEEEECCCCHHHHCCCC
LPAEPAKTAHFCSMCGPKFCSMRITADIRVYAAKHGLDTEEAIEMGMTEKSAEFAEHGNR
CCCCCCCHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCE
VYLPLTQ
EEEEECC
>Mature Secondary Structure 
TETLSKTTEPSVTTGPIPGSSKAYREVANPDGGPSLRVPFRRVHLSTGAHFDLYDTSGP
CCCCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCCEECCCEEEEECCCCEEEEECCCCC
YTDPDAVINLTAGLPPRPGVIRDRGTQLQRARAGEITAEMAFIADREGMPAELVRVEVAL
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCHHHHHHEEECCCCCCHHHHEEEHHH
GRAVIPANHNHPEIEPMIIGKAFAVKVNANIGNSAVTSSIAEEIDKMVWATRWGADTIMD
CCEEECCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTKLTWEIYRDTVIEQCEQGVDY
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCE
MTVHAGVLLRYVLLTAKRVTGIVSRGGSIMASWCLANHRESFLYTNFAELCDIFARYDVT
EEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCEE
FSLGDGLRPGSIADANDTAQFAELRTLGELSKIAKVHGAQVMIEGPGHIPMHKIVENVRL
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHH
EEELCEEAPFYTLGPLATDIAPAYDHITSAIGAAVIAQAGTAMLCYVTPKEHLGLPDRKD
HHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCC
VKDGVIAYKIAAHAADLAKGYPRAQERDDALSTARFEFRWNDQFALSLDPPTAREFHDET
CCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECCEEEEEECCCCHHHHCCCC
LPAEPAKTAHFCSMCGPKFCSMRITADIRVYAAKHGLDTEEAIEMGMTEKSAEFAEHGNR
CCCCCCCHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCE
VYLPLTQ
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]