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The map label for this gene is metS [H]

Identifier: 221229512

GI number: 221229512

Start: 312224

End: 313837

Strand: Reverse

Name: metS [H]

Synonym: MLBr_00238

Alternate gene names: 221229512

Gene position: 313837-312224 (Counterclockwise)

Preceding gene: 221229518

Following gene: 221229511

Centisome position: 9.6

GC content: 58.61

Gene sequence:

>1614_bases
ATGAGGCCCTATTACATCACCACTGCCATCGCGTATCCCAACGCTGCGCCGCACATTGGTCACGCGTACGAATACATCGC
CACCGACGCGATTGCCCGCTTCAAGCGGCTTGACGGCTTAGATGTGCGCTTCCTGACCGGAACCGACGAGCACGGCCTGA
AGGTCGCACAGGCCGCCGAAGCAGCCGGCGTGCCGACAGCGCAACTTGCCAGGCGCAATTCGGGTGTATTTCAGCGCATG
CAAGAGGCACTCCACATTTCCTTCGATCGGTTCATCCGCACCACCGACGCCGACCACTACAAGGCAGCTAAGGAAATCTG
GCGGCGAATGGATGCGGCCGGCGACATCTACCTAGGTACCTATTCCGGGTGGTACTCGGTGCGCGACGAGAGGTTTTTCG
TCGACTCGGAGACCAAACTTCTCGATAACGGCATCCGAGTAGCAGTAGAAACCGGCACACCAGTCACCTGGACTGAGAAA
GAGCAGACCTACTTTTTCCGACTGTCGGCATATGTCGACAAATTGCTAGCCCACTACGACGCCAACCCCGACTTCATCGG
ACCCGAGGTGCGTCGCAACGAAGTGATCAGCTTTGTCTCCGGTGGGCTGGAAGATTTCTCAATCTCGCGCACTTCATTCG
ACTGGGGTGTACAGGTCCCAGAACATCCTGACCATGTCATGTACGTCTGGATCGACGCGCTAACCAACTACCTCACCGGA
GCCGGGTTCCCGGACACCGACTCGGAGTTGTTCGGTCGTTACTGGCCCGCTAATCTACACATGATCGGCAAAGACATCAT
CAGGTTCCATGCCGTATACTGGCCGGCGTTTTTGATGTCAGCCGGAATCGAGCTGCCACGAAGGATTTTCGCACACGGGT
TCTTACACAACCACGGCGAGAAGATGAGCAAGTCGGTGGGCAATATCGTTGACCCGATGGCGCTGGTACAAACGTTCGGC
GTGGATCAGGTTCGATATTTTCTGTTGCGCGAGATCCCGTTCGGCCAGGACGGCAACTACAGCGAAGAGGCGATCATCAC
CCGGATGAACACCGACCTGGCCAACGAATTCGGCAATCTGGCGCAGCGCTCGTTGTCGATGGTTGCCAAGAACCTGGGCG
GCGTAGTGCCTGAACCCAGTGAGTTCACCTCCGCCGACACTGCGCTTCTCACGACGGCCGACGGCTTGCTGGAGCGGGTG
CGCGGCAACTTTGACGGACAGGCGATGAACCTGGCCCTGGAAGCTATTTGGCTGATGCTCGGCGAAGCTAACAAGTACTT
TTCGTCGCAACAGCCCTGGATCTTGCGTAAGAGCGAGTCTGAAGCGGATCAGGCCAGGTTCCGTACCGTCCTCTACACGA
CTTGCGAGGTGGTGCGGATTGCGGCGTTGCTTGTCCAGCCCGTTATGCCGGAATCGGCCGGCAAGATGTTGGACTTGCTT
GGCCAGGAGGAAGACCAACGGGCATTCACTGCCGTCAGTGTGCGGCTGGCCCCCGGCACGGTGCTGCCGCCACCCACCGG
TGTGTTCCCCCGCTACCAGCCTTCCGAAATCGAGGGAGCGGACCCCGTGAAGTCGAGCAGCAAGCGGAGAGAACACAATA
AGCGACGGGAGTGA

Upstream 100 bases:

>100_bases
GGCATCGACCAACGGTGACAGGGGTTCCGGCGCAGGAGGCGCGTCTTGTTGTCTAACTAGGCGGTTAGAACTCACGCACA
CACAATAGGGTGGAGTCTCG

Downstream 100 bases:

>100_bases
GACGAACAATCGCACGTAGTTGACGCCAAACGAGACAAGCACCCGCAATCGAGTGAGCGGAACGGAGTGATCCATGGCCT
CCGACGACCCGGTCACTGGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MRPYYITTAIAYPNAAPHIGHAYEYIATDAIARFKRLDGLDVRFLTGTDEHGLKVAQAAEAAGVPTAQLARRNSGVFQRM
QEALHISFDRFIRTTDADHYKAAKEIWRRMDAAGDIYLGTYSGWYSVRDERFFVDSETKLLDNGIRVAVETGTPVTWTEK
EQTYFFRLSAYVDKLLAHYDANPDFIGPEVRRNEVISFVSGGLEDFSISRTSFDWGVQVPEHPDHVMYVWIDALTNYLTG
AGFPDTDSELFGRYWPANLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNHGEKMSKSVGNIVDPMALVQTFG
VDQVRYFLLREIPFGQDGNYSEEAIITRMNTDLANEFGNLAQRSLSMVAKNLGGVVPEPSEFTSADTALLTTADGLLERV
RGNFDGQAMNLALEAIWLMLGEANKYFSSQQPWILRKSESEADQARFRTVLYTTCEVVRIAALLVQPVMPESAGKMLDLL
GQEEDQRAFTAVSVRLAPGTVLPPPTGVFPRYQPSEIEGADPVKSSSKRREHNKRRE

Sequences:

>Translated_537_residues
MRPYYITTAIAYPNAAPHIGHAYEYIATDAIARFKRLDGLDVRFLTGTDEHGLKVAQAAEAAGVPTAQLARRNSGVFQRM
QEALHISFDRFIRTTDADHYKAAKEIWRRMDAAGDIYLGTYSGWYSVRDERFFVDSETKLLDNGIRVAVETGTPVTWTEK
EQTYFFRLSAYVDKLLAHYDANPDFIGPEVRRNEVISFVSGGLEDFSISRTSFDWGVQVPEHPDHVMYVWIDALTNYLTG
AGFPDTDSELFGRYWPANLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNHGEKMSKSVGNIVDPMALVQTFG
VDQVRYFLLREIPFGQDGNYSEEAIITRMNTDLANEFGNLAQRSLSMVAKNLGGVVPEPSEFTSADTALLTTADGLLERV
RGNFDGQAMNLALEAIWLMLGEANKYFSSQQPWILRKSESEADQARFRTVLYTTCEVVRIAALLVQPVMPESAGKMLDLL
GQEEDQRAFTAVSVRLAPGTVLPPPTGVFPRYQPSEIEGADPVKSSSKRREHNKRRE
>Mature_537_residues
MRPYYITTAIAYPNAAPHIGHAYEYIATDAIARFKRLDGLDVRFLTGTDEHGLKVAQAAEAAGVPTAQLARRNSGVFQRM
QEALHISFDRFIRTTDADHYKAAKEIWRRMDAAGDIYLGTYSGWYSVRDERFFVDSETKLLDNGIRVAVETGTPVTWTEK
EQTYFFRLSAYVDKLLAHYDANPDFIGPEVRRNEVISFVSGGLEDFSISRTSFDWGVQVPEHPDHVMYVWIDALTNYLTG
AGFPDTDSELFGRYWPANLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNHGEKMSKSVGNIVDPMALVQTFG
VDQVRYFLLREIPFGQDGNYSEEAIITRMNTDLANEFGNLAQRSLSMVAKNLGGVVPEPSEFTSADTALLTTADGLLERV
RGNFDGQAMNLALEAIWLMLGEANKYFSSQQPWILRKSESEADQARFRTVLYTTCEVVRIAALLVQPVMPESAGKMLDLL
GQEEDQRAFTAVSVRLAPGTVLPPPTGVFPRYQPSEIEGADPVKSSSKRREHNKRRE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=508, Percent_Identity=38.1889763779528, Blast_Score=340, Evalue=3e-93,
Organism=Homo sapiens, GI14043022, Length=515, Percent_Identity=26.4077669902913, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788432, Length=502, Percent_Identity=26.2948207171315, Blast_Score=178, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI212645096, Length=520, Percent_Identity=35.9615384615385, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71992781, Length=414, Percent_Identity=34.2995169082126, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17540794, Length=561, Percent_Identity=24.5989304812834, Blast_Score=145, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=32.3364485981308, Blast_Score=283, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6321704, Length=541, Percent_Identity=22.735674676525, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21358483, Length=518, Percent_Identity=38.030888030888, Blast_Score=351, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24655311, Length=571, Percent_Identity=24.5183887915937, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 60433; Mature: 60433

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPYYITTAIAYPNAAPHIGHAYEYIATDAIARFKRLDGLDVRFLTGTDEHGLKVAQAAE
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
AAGVPTAQLARRNSGVFQRMQEALHISFDRFIRTTDADHYKAAKEIWRRMDAAGDIYLGT
HCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEC
YSGWYSVRDERFFVDSETKLLDNGIRVAVETGTPVTWTEKEQTYFFRLSAYVDKLLAHYD
CCCEEEECCCEEEECCCHHHHCCCCEEEEECCCCCEECCCCCEEEEHHHHHHHHHHHHCC
ANPDFIGPEVRRNEVISFVSGGLEDFSISRTSFDWGVQVPEHPDHVMYVWIDALTNYLTG
CCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCC
AGFPDTDSELFGRYWPANLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNHGE
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KMSKSVGNIVDPMALVQTFGVDQVRYFLLREIPFGQDGNYSEEAIITRMNTDLANEFGNL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHEEEECCHHHHHHHHHH
AQRSLSMVAKNLGGVVPEPSEFTSADTALLTTADGLLERVRGNFDGQAMNLALEAIWLML
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GEANKYFSSQQPWILRKSESEADQARFRTVLYTTCEVVRIAALLVQPVMPESAGKMLDLL
HCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GQEEDQRAFTAVSVRLAPGTVLPPPTGVFPRYQPSEIEGADPVKSSSKRREHNKRRE
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRPYYITTAIAYPNAAPHIGHAYEYIATDAIARFKRLDGLDVRFLTGTDEHGLKVAQAAE
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
AAGVPTAQLARRNSGVFQRMQEALHISFDRFIRTTDADHYKAAKEIWRRMDAAGDIYLGT
HCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEC
YSGWYSVRDERFFVDSETKLLDNGIRVAVETGTPVTWTEKEQTYFFRLSAYVDKLLAHYD
CCCEEEECCCEEEECCCHHHHCCCCEEEEECCCCCEECCCCCEEEEHHHHHHHHHHHHCC
ANPDFIGPEVRRNEVISFVSGGLEDFSISRTSFDWGVQVPEHPDHVMYVWIDALTNYLTG
CCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCC
AGFPDTDSELFGRYWPANLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNHGE
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KMSKSVGNIVDPMALVQTFGVDQVRYFLLREIPFGQDGNYSEEAIITRMNTDLANEFGNL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHEEEECCHHHHHHHHHH
AQRSLSMVAKNLGGVVPEPSEFTSADTALLTTADGLLERVRGNFDGQAMNLALEAIWLML
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GEANKYFSSQQPWILRKSESEADQARFRTVLYTTCEVVRIAALLVQPVMPESAGKMLDLL
HCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GQEEDQRAFTAVSVRLAPGTVLPPPTGVFPRYQPSEIEGADPVKSSSKRREHNKRRE
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]