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The map label for this gene is pth

Identifier: 221229518

GI number: 221229518

Start: 319981

End: 320580

Strand: Reverse

Name: pth

Synonym: MLBr_00244

Alternate gene names: 221229518

Gene position: 320580-319981 (Counterclockwise)

Preceding gene: 221229519

Following gene: 221229512

Centisome position: 9.81

GC content: 62.0

Gene sequence:

>600_bases
GTGGCCGAACCCACATTGGCCAGCGGGTGGTACCCCCGGTTGGTGGTCGGCCTAGGCAACCCCGGCAAAAACTATGGCCG
TACCCGACACAACGTTGGGTTTATGGTTGCCAACCTGCTCGCTGTGCGACTGGGCTCGAAATTCGAGGTGCATAAGCGTT
CTGGCGCTGACGTCGTCAACGGCCGTCTGGCGGGGTGTTCGATGTTGGTGGCCAAGCCACGCAACTACATGAACGAGTCT
GGCCAGCAGGTGGGGCTACTAGCGAAATTATACTCGGTAACCCCCGCGGACATCATCATCGTCCACGACGACCTCGACCT
CGACTTCGGTCGTATCCGGCTCAAACTCGGCGGCGGCGAGGGTGGCCACAACGGGCTGCGCTCGGTGGCGGCCGCGTTGG
GTACCAAAGACTTTCAGCGGGTGCGCATCGGGATTGGTCGTCCGACTGGCCGCAAAGATCCGGCGTCGTTTGTGTTGGAG
AACTTCACCACTGCCGAACGCATGCAGGTTCCCACCATCTGCAAGCGGGCCGCCGACGCCACCGAATTGTTGGTCGGATT
GGGCCTAGAGCCCGCACAGAACCACGTGCACGCCTGGTAG

Upstream 100 bases:

>100_bases
AACCAAGGCCAAATACGCAGGCGAGGCACACGAAGCTCCGGAAGTAGGAACAGCCGAAAACAAGACCGCCGCAACCGAGT
CCGAGTAGGCGGTTCGCGCG

Downstream 100 bases:

>100_bases
CAGCCGGTCGCGTCCTCAGCTGCACCCCACGAGGGCGCATTTCCGCATTTCCGTAAAACGACACGCCGGAAACCATGGCA
GTTTTCATCAGCTCGGCTGC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MAEPTLASGWYPRLVVGLGNPGKNYGRTRHNVGFMVANLLAVRLGSKFEVHKRSGADVVNGRLAGCSMLVAKPRNYMNES
GQQVGLLAKLYSVTPADIIIVHDDLDLDFGRIRLKLGGGEGGHNGLRSVAAALGTKDFQRVRIGIGRPTGRKDPASFVLE
NFTTAERMQVPTICKRAADATELLVGLGLEPAQNHVHAW

Sequences:

>Translated_199_residues
MAEPTLASGWYPRLVVGLGNPGKNYGRTRHNVGFMVANLLAVRLGSKFEVHKRSGADVVNGRLAGCSMLVAKPRNYMNES
GQQVGLLAKLYSVTPADIIIVHDDLDLDFGRIRLKLGGGEGGHNGLRSVAAALGTKDFQRVRIGIGRPTGRKDPASFVLE
NFTTAERMQVPTICKRAADATELLVGLGLEPAQNHVHAW
>Mature_198_residues
AEPTLASGWYPRLVVGLGNPGKNYGRTRHNVGFMVANLLAVRLGSKFEVHKRSGADVVNGRLAGCSMLVAKPRNYMNESG
QQVGLLAKLYSVTPADIIIVHDDLDLDFGRIRLKLGGGEGGHNGLRSVAAALGTKDFQRVRIGIGRPTGRKDPASFVLEN
FTTAERMQVPTICKRAADATELLVGLGLEPAQNHVHAW

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=38.6904761904762, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1787455, Length=188, Percent_Identity=37.2340425531915, Blast_Score=105, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321983, Length=181, Percent_Identity=34.2541436464088, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_MYCLB (B8ZU62)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502934.1
- ProteinModelPortal:   B8ZU62
- SMR:   B8ZU62
- EnsemblBacteria:   EBMYCT00000087132
- GeneID:   7326329
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017462
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21438; Mature: 21307

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPTLASGWYPRLVVGLGNPGKNYGRTRHNVGFMVANLLAVRLGSKFEVHKRSGADVVN
CCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
GRLAGCSMLVAKPRNYMNESGQQVGLLAKLYSVTPADIIIVHDDLDLDFGRIRLKLGGGE
CCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCC
GGHNGLRSVAAALGTKDFQRVRIGIGRPTGRKDPASFVLENFTTAERMQVPTICKRAADA
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCHHHHCCHHHHHHHCCH
TELLVGLGLEPAQNHVHAW
HHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure 
AEPTLASGWYPRLVVGLGNPGKNYGRTRHNVGFMVANLLAVRLGSKFEVHKRSGADVVN
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
GRLAGCSMLVAKPRNYMNESGQQVGLLAKLYSVTPADIIIVHDDLDLDFGRIRLKLGGGE
CCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCC
GGHNGLRSVAAALGTKDFQRVRIGIGRPTGRKDPASFVLENFTTAERMQVPTICKRAADA
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCHHHHCCHHHHHHHCCH
TELLVGLGLEPAQNHVHAW
HHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA