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The map label for this gene is folE

Identifier: 221229498

GI number: 221229498

Start: 296097

End: 296714

Strand: Direct

Name: folE

Synonym: MLBr_00223

Alternate gene names: 221229498

Gene position: 296097-296714 (Clockwise)

Preceding gene: 221229497

Following gene: 221229499

Centisome position: 9.06

GC content: 61.17

Gene sequence:

>618_bases
ATGGCCTTACTGGATTTAGGGCTGGAAAGCACCGCGGTCCCGCGCATCCGGGTTTTTGACCAGCAGCGTGCCGAGGCCGC
GATTCGCGAGTTGCTGTACGCGATTGGCGAGGACCCGGATAGGGAGGGTTTGGCCGACACCCCGGCTCGCGTGGCCCGCG
CATGCCGCGAGCTGTTCTCTGGGCTCTACACCGACCCGCAAACTGTGCTGAACACCATGTTCGACGAAGAACACAATGAA
CTGGTGATCGTCAAAGAGATTCCGATGTACTCTACCTGCGAACACCATTTGGTGTCTTTCCATGGTGTGGCGCACATCGG
CTATCTTCCAGGCGCGGACGGTAGGGTAACCGGGCTTTCGAAGATCGCTCGGCTAGTGGACCTCTACGCCAAACGGCCAC
AAGTGCAGGAGCGGCTCACCAGCCAGATCGCCGACGCCTTGGTCAGCAAGCTCGATCCACGCGGGGTCATCATCGTCGTC
GAGGCCGAGCACCTGTGCATGGCAATGCGCGGTGTCCGCAAGCCTGGCGCGATCACGACGACGTCGGCGGTGCGCGGTCA
GTTCAAAACTGATGCTGCTTCTCGTGCCGAAGCGCTCGGCCTCATCCTGCGTAAGTGA

Upstream 100 bases:

>100_bases
GGTCAATCCGCCCCGGACGCGGGTAAGCCCCCTGCACAGTTGGACGAGGGCGTGAGCCCCTCCAACCCACCGGCGCACGG
CTGACGAAACGGAGAATTCG

Downstream 100 bases:

>100_bases
GTTTGGCGCCAGTGCAGGTTATTGGGGTTTTGAACGTCACTGACAATTCGTTCTCAGATGGCGGACGTTACCTTGATCCT
GACGATGCTGTCCAGCACGG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MALLDLGLESTAVPRIRVFDQQRAEAAIRELLYAIGEDPDREGLADTPARVARACRELFSGLYTDPQTVLNTMFDEEHNE
LVIVKEIPMYSTCEHHLVSFHGVAHIGYLPGADGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALVSKLDPRGVIIVV
EAEHLCMAMRGVRKPGAITTTSAVRGQFKTDAASRAEALGLILRK

Sequences:

>Translated_205_residues
MALLDLGLESTAVPRIRVFDQQRAEAAIRELLYAIGEDPDREGLADTPARVARACRELFSGLYTDPQTVLNTMFDEEHNE
LVIVKEIPMYSTCEHHLVSFHGVAHIGYLPGADGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALVSKLDPRGVIIVV
EAEHLCMAMRGVRKPGAITTTSAVRGQFKTDAASRAEALGLILRK
>Mature_204_residues
ALLDLGLESTAVPRIRVFDQQRAEAAIRELLYAIGEDPDREGLADTPARVARACRELFSGLYTDPQTVLNTMFDEEHNEL
VIVKEIPMYSTCEHHLVSFHGVAHIGYLPGADGRVTGLSKIARLVDLYAKRPQVQERLTSQIADALVSKLDPRGVIIVVE
AEHLCMAMRGVRKPGAITTTSAVRGQFKTDAASRAEALGLILRK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=178, Percent_Identity=51.685393258427, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI4503949, Length=178, Percent_Identity=51.685393258427, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI66932970, Length=138, Percent_Identity=52.8985507246377, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=52.8985507246377, Blast_Score=137, Evalue=8e-33,
Organism=Escherichia coli, GI1788476, Length=207, Percent_Identity=38.1642512077295, Blast_Score=114, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=48.8888888888889, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=186, Percent_Identity=48.9247311827957, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=48.3333333333333, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=48.3333333333333, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=48.3333333333333, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_MYCLB (B8ZU42)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502914.1
- ProteinModelPortal:   B8ZU42
- SMR:   B8ZU42
- EnsemblBacteria:   EBMYCT00000085753
- GeneID:   7325313
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016683
- HOGENOM:   HBG370191
- OMA:   ARFMSMI
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22531; Mature: 22400

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLDLGLESTAVPRIRVFDQQRAEAAIRELLYAIGEDPDREGLADTPARVARACRELFS
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
GLYTDPQTVLNTMFDEEHNELVIVKEIPMYSTCEHHLVSFHGVAHIGYLPGADGRVTGLS
HCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
KIARLVDLYAKRPQVQERLTSQIADALVSKLDPRGVIIVVEAEHLCMAMRGVRKPGAITT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCCEEE
TSAVRGQFKTDAASRAEALGLILRK
HHHHHCCHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALLDLGLESTAVPRIRVFDQQRAEAAIRELLYAIGEDPDREGLADTPARVARACRELFS
CEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
GLYTDPQTVLNTMFDEEHNELVIVKEIPMYSTCEHHLVSFHGVAHIGYLPGADGRVTGLS
HCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
KIARLVDLYAKRPQVQERLTSQIADALVSKLDPRGVIIVVEAEHLCMAMRGVRKPGAITT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCCEEE
TSAVRGQFKTDAASRAEALGLILRK
HHHHHCCHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA