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The map label for this gene is purH

Identifier: 221229461

GI number: 221229461

Start: 224643

End: 226226

Strand: Direct

Name: purH

Synonym: MLBr_00161

Alternate gene names: 221229461

Gene position: 224643-226226 (Clockwise)

Preceding gene: 221229460

Following gene: 221229463

Centisome position: 6.87

GC content: 61.05

Gene sequence:

>1584_bases
ATGAGCAGTGACGGCGAGCGAGACATGGCAAGAAAACCGATTCGCCGCGCGTTGATCAGTGTATACGACAAGACCGGTCT
GATTGACCTTGCCCAGGGTCTGAATGCGGCGGGTGTCGATATCGTCTCGACCGGATCGACCGCGAAAGCCATTGCCGATC
AAGGGATTGCGGTGACACCTGTGGAGGAACTGACCGGGTTCCCCGAGGTACTTGACGGCCGAGTCAAGACATTGCACCCT
CGGGTTCATGCCGGACTGTTGGCCGACTTGCGTAAACCTGAACACGCCGCGGCGCTTGAACAACTCGGAATTGAGGCATT
CGAACTAGTTGTCGTCAACTTGTATCCGTTCAGCCAGACGGTCAAGTCCGGTGCGACTGTCGACGAATGTGTCGAACAGA
TCGATATTGGCGGATCTTCGATGGTGCGAGCGGCCGCAAAGAACCATCCCAGTGTGGCGGTGGTCACCGACCCGCTTGGG
TACGTTGGCGTGCTTGCCGCGGTGCAGGGCGGTGGGTTCACCCTTGCCGAGCGTAAAATGCTGGCTTCGATGGCATTTCA
GCACATAGCGGAATACGAAATCGCCGTCGCTAGTTGGATGCAGTCGACGCTGGCGCCCGAACAGCCACCAACGGCGTTTC
CGCAGTGGTTTGGCCGGAGCTGGCGTCGCTCAGCAATTCTGCGCTACGGGGAGAACCCGCACCAGCAGGCGGCCTTGTAC
AGCGACCCCAGTGCCTGTCCTGGCTTGGCCCAGGCCGAGCAGCTGCATGGGAAAAACATGTCCTACAACAACTTCACTGA
CGCAGACGCAGCCTGGCGTGCTGCCTTCGACCACGAGCAGTCGTGCGTGGCAATCATCAAACATGCCAACCCGTGCGGGA
TTGCAATATCGTCGCTTTCGGTCGCCGACGCACATCGCAAGGCTCACGAATGCGACCCGTTGAGTGCCTACGGCGGAGTT
ATTGCCGCCAATACTGAGGTCAGCCTTGAGATGGCAGAGTACGTGAGCACCATCTTCACCGAGGTTATCGTTGCACCCTC
CTACGCACCAGGCGCCGTCGACGTGCTGTCCTGCAAAAAAAACGTCCGAGTCTTGGTGGCCTCCGCACCGCTACGCGGTG
GTAGCGAGCTACGGCCGGTCAGCGGGGGACTGTTGATCCAGCAACCCGATCAGCTTGACACGGCCGGTGACAACCCAGCG
AACTGGACCTTGGCAACCGGATCACCGGCCGGCCCGGCGACGTTGACGGACTTGGTTTTTGCGTGGCGTGCCTGCCGCGC
GGTGAAGTCGAACGCGATTGTGATCGCCGCCGACGGCGCCACCATAGGTGTTGGCATGGGTCAGGTTAACCGAGTCGACG
CCGCTCGACTTGCCGTTGAGCGTGGCGGCGACCGGGTCCGTGGCGCAGTGGTAGCTTCCGATGCGTTCTTTCCCTTTGCT
GACGGACTTCAGACGCTGGCCGCGGCCGGAGTCACGGCGATAGTGCACCCCGGGGGCTCCGTGCGTGACGCGGAGGTTAC
CGCAGCCGCGACCAAAGCCGGGGTCACTTTGTATCTCACCGGAGTTCGCCACTTCGTGCACTAG

Upstream 100 bases:

>100_bases
TCGAACGACGGCTGTTGGTTGACGTGGTGGCCGTGATCGCGACAGGTGGCGTGACCTTGGTCGGCAGAAAAGCGACGATC
GGACGAAAGGCAACCATAGA

Downstream 100 bases:

>100_bases
ACTTCATGCGTTAAATTTGAGTCAAGCCCGGGGCTGGCGCAGCACCCGTATCGGGGTGGGGGGTACGCAATAGCGCTTGA
CTCGGCCATGTGCTGTGACA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSSDGERDMARKPIRRALISVYDKTGLIDLAQGLNAAGVDIVSTGSTAKAIADQGIAVTPVEELTGFPEVLDGRVKTLHP
RVHAGLLADLRKPEHAAALEQLGIEAFELVVVNLYPFSQTVKSGATVDECVEQIDIGGSSMVRAAAKNHPSVAVVTDPLG
YVGVLAAVQGGGFTLAERKMLASMAFQHIAEYEIAVASWMQSTLAPEQPPTAFPQWFGRSWRRSAILRYGENPHQQAALY
SDPSACPGLAQAEQLHGKNMSYNNFTDADAAWRAAFDHEQSCVAIIKHANPCGIAISSLSVADAHRKAHECDPLSAYGGV
IAANTEVSLEMAEYVSTIFTEVIVAPSYAPGAVDVLSCKKNVRVLVASAPLRGGSELRPVSGGLLIQQPDQLDTAGDNPA
NWTLATGSPAGPATLTDLVFAWRACRAVKSNAIVIAADGATIGVGMGQVNRVDAARLAVERGGDRVRGAVVASDAFFPFA
DGLQTLAAAGVTAIVHPGGSVRDAEVTAAATKAGVTLYLTGVRHFVH

Sequences:

>Translated_527_residues
MSSDGERDMARKPIRRALISVYDKTGLIDLAQGLNAAGVDIVSTGSTAKAIADQGIAVTPVEELTGFPEVLDGRVKTLHP
RVHAGLLADLRKPEHAAALEQLGIEAFELVVVNLYPFSQTVKSGATVDECVEQIDIGGSSMVRAAAKNHPSVAVVTDPLG
YVGVLAAVQGGGFTLAERKMLASMAFQHIAEYEIAVASWMQSTLAPEQPPTAFPQWFGRSWRRSAILRYGENPHQQAALY
SDPSACPGLAQAEQLHGKNMSYNNFTDADAAWRAAFDHEQSCVAIIKHANPCGIAISSLSVADAHRKAHECDPLSAYGGV
IAANTEVSLEMAEYVSTIFTEVIVAPSYAPGAVDVLSCKKNVRVLVASAPLRGGSELRPVSGGLLIQQPDQLDTAGDNPA
NWTLATGSPAGPATLTDLVFAWRACRAVKSNAIVIAADGATIGVGMGQVNRVDAARLAVERGGDRVRGAVVASDAFFPFA
DGLQTLAAAGVTAIVHPGGSVRDAEVTAAATKAGVTLYLTGVRHFVH
>Mature_526_residues
SSDGERDMARKPIRRALISVYDKTGLIDLAQGLNAAGVDIVSTGSTAKAIADQGIAVTPVEELTGFPEVLDGRVKTLHPR
VHAGLLADLRKPEHAAALEQLGIEAFELVVVNLYPFSQTVKSGATVDECVEQIDIGGSSMVRAAAKNHPSVAVVTDPLGY
VGVLAAVQGGGFTLAERKMLASMAFQHIAEYEIAVASWMQSTLAPEQPPTAFPQWFGRSWRRSAILRYGENPHQQAALYS
DPSACPGLAQAEQLHGKNMSYNNFTDADAAWRAAFDHEQSCVAIIKHANPCGIAISSLSVADAHRKAHECDPLSAYGGVI
AANTEVSLEMAEYVSTIFTEVIVAPSYAPGAVDVLSCKKNVRVLVASAPLRGGSELRPVSGGLLIQQPDQLDTAGDNPAN
WTLATGSPAGPATLTDLVFAWRACRAVKSNAIVIAADGATIGVGMGQVNRVDAARLAVERGGDRVRGAVVASDAFFPFAD
GLQTLAAAGVTAIVHPGGSVRDAEVTAAATKAGVTLYLTGVRHFVH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=35.2577319587629, Blast_Score=228, Evalue=8e-60,
Organism=Escherichia coli, GI1790439, Length=532, Percent_Identity=43.2330827067669, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71985564, Length=476, Percent_Identity=34.453781512605, Blast_Score=221, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6323056, Length=483, Percent_Identity=32.5051759834368, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323768, Length=482, Percent_Identity=32.5726141078838, Blast_Score=208, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24649832, Length=475, Percent_Identity=33.2631578947368, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_MYCLB (B8ZU05)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502877.1
- ProteinModelPortal:   B8ZU05
- SMR:   B8ZU05
- EnsemblBacteria:   EBMYCT00000087150
- GeneID:   7325042
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55230; Mature: 55099

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDGERDMARKPIRRALISVYDKTGLIDLAQGLNAAGVDIVSTGSTAKAIADQGIAVTP
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEECCCHHHHHHCCCCEECC
VEELTGFPEVLDGRVKTLHPRVHAGLLADLRKPEHAAALEQLGIEAFELVVVNLYPFSQT
HHHHCCCHHHHCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEECCCHHH
VKSGATVDECVEQIDIGGSSMVRAAAKNHPSVAVVTDPLGYVGVLAAVQGGGFTLAERKM
HHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEHHHHHH
LASMAFQHIAEYEIAVASWMQSTLAPEQPPTAFPQWFGRSWRRSAILRYGENPHQQAALY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHCCCCHHHCCEEC
SDPSACPGLAQAEQLHGKNMSYNNFTDADAAWRAAFDHEQSCVAIIKHANPCGIAISSLS
CCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHEECCCCCCEEEECCH
VADAHRKAHECDPLSAYGGVIAANTEVSLEMAEYVSTIFTEVIVAPSYAPGAVDVLSCKK
HHHHHHHHCCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
NVRVLVASAPLRGGSELRPVSGGLLIQQPDQLDTAGDNPANWTLATGSPAGPATLTDLVF
CCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
AWRACRAVKSNAIVIAADGATIGVGMGQVNRVDAARLAVERGGDRVRGAVVASDAFFPFA
HHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
DGLQTLAAAGVTAIVHPGGSVRDAEVTAAATKAGVTLYLTGVRHFVH
HHHHHHHHCCCEEEECCCCCCCCCCEEHHHHHCCEEEEEECHHHHCC
>Mature Secondary Structure 
SSDGERDMARKPIRRALISVYDKTGLIDLAQGLNAAGVDIVSTGSTAKAIADQGIAVTP
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEECCCHHHHHHCCCCEECC
VEELTGFPEVLDGRVKTLHPRVHAGLLADLRKPEHAAALEQLGIEAFELVVVNLYPFSQT
HHHHCCCHHHHCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEECCCHHH
VKSGATVDECVEQIDIGGSSMVRAAAKNHPSVAVVTDPLGYVGVLAAVQGGGFTLAERKM
HHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEHHHHHH
LASMAFQHIAEYEIAVASWMQSTLAPEQPPTAFPQWFGRSWRRSAILRYGENPHQQAALY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHCCCCHHHCCEEC
SDPSACPGLAQAEQLHGKNMSYNNFTDADAAWRAAFDHEQSCVAIIKHANPCGIAISSLS
CCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHEECCCCCCEEEECCH
VADAHRKAHECDPLSAYGGVIAANTEVSLEMAEYVSTIFTEVIVAPSYAPGAVDVLSCKK
HHHHHHHHCCCCCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
NVRVLVASAPLRGGSELRPVSGGLLIQQPDQLDTAGDNPANWTLATGSPAGPATLTDLVF
CCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
AWRACRAVKSNAIVIAADGATIGVGMGQVNRVDAARLAVERGGDRVRGAVVASDAFFPFA
HHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
DGLQTLAAAGVTAIVHPGGSVRDAEVTAAATKAGVTLYLTGVRHFVH
HHHHHHHHCCCEEEECCCCCCCCCCEEHHHHHCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA