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The map label for this gene is purN [H]

Identifier: 221229460

GI number: 221229460

Start: 223999

End: 224646

Strand: Direct

Name: purN [H]

Synonym: MLBr_00160

Alternate gene names: 221229460

Gene position: 223999-224646 (Clockwise)

Preceding gene: 221229459

Following gene: 221229461

Centisome position: 6.85

GC content: 60.8

Gene sequence:

>648_bases
GTGCAGGAACCGCTCTGTGTGTCCCCCAGTGCACCATCGCGGGTGGTGGTGTTGGCCTCGGGCACTGGTTCGTTACTGGG
TTCACTGATCGACGCCTCTGTTGGCAATTACCCGGCCCGGGTGGTGGCCGTCGGTGTCGACCGTGACTGCGGGGCGACAA
AGATCGCCAAGGCAGCATCAGTGCCGACCTTTACCGTCCGACTCGCTGACCCCCCCGGACGGGATGCCTGGGACGCCAAG
ATCACCGAAGCCGTCGCATCGTACAAGCCCGATCTGGTCGTCTTGGCAGGATTTATGAGAATCCTTGGGCCGCAGTTTCT
TGCACGATTCTTTGGTCGCATCGTCAATACCCATCCGGCGCTACTCCCGGCATTTCCGGGTACGCATGGCGTAGCTGACG
CGTTGGCCTACGGTGTGAAGGTCACAGGCGCTACGGTGCACCTAGTGGATGCCGGCACTGACACTGGGCCGATATTAGCG
CAGCAATCCGTTCCGGTGCTCGACGGTGACGACACCGCGGCTTTGCATGAACGCATCAAGGTCATCGAACGACGGCTGTT
GGTTGACGTGGTGGCCGTGATCGCGACAGGTGGCGTGACCTTGGTCGGCAGAAAAGCGACGATCGGACGAAAGGCAACCA
TAGAATGA

Upstream 100 bases:

>100_bases
TCAACGTTCCTTTCGATCTCTCCGGAGAGGACGAAATACCCAAGGCTGAGCCCGGCGAGGCAGCGGATTAGCGCATCGTC
GCCAAGACTACGCTCGCAGT

Downstream 100 bases:

>100_bases
GCAGTGACGGCGAGCGAGACATGGCAAGAAAACCGATTCGCCGCGCGTTGATCAGTGTATACGACAAGACCGGTCTGATT
GACCTTGCCCAGGGTCTGAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MQEPLCVSPSAPSRVVVLASGTGSLLGSLIDASVGNYPARVVAVGVDRDCGATKIAKAASVPTFTVRLADPPGRDAWDAK
ITEAVASYKPDLVVLAGFMRILGPQFLARFFGRIVNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILA
QQSVPVLDGDDTAALHERIKVIERRLLVDVVAVIATGGVTLVGRKATIGRKATIE

Sequences:

>Translated_215_residues
MQEPLCVSPSAPSRVVVLASGTGSLLGSLIDASVGNYPARVVAVGVDRDCGATKIAKAASVPTFTVRLADPPGRDAWDAK
ITEAVASYKPDLVVLAGFMRILGPQFLARFFGRIVNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILA
QQSVPVLDGDDTAALHERIKVIERRLLVDVVAVIATGGVTLVGRKATIGRKATIE
>Mature_215_residues
MQEPLCVSPSAPSRVVVLASGTGSLLGSLIDASVGNYPARVVAVGVDRDCGATKIAKAASVPTFTVRLADPPGRDAWDAK
ITEAVASYKPDLVVLAGFMRILGPQFLARFFGRIVNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILA
QQSVPVLDGDDTAALHERIKVIERRLLVDVVAVIATGGVTLVGRKATIGRKATIE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=191, Percent_Identity=37.17277486911, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI209869995, Length=191, Percent_Identity=37.17277486911, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI209869993, Length=191, Percent_Identity=37.17277486911, Blast_Score=124, Evalue=6e-29,
Organism=Escherichia coli, GI1788846, Length=197, Percent_Identity=35.0253807106599, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787483, Length=161, Percent_Identity=34.7826086956522, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=36.7567567567568, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24582400, Length=190, Percent_Identity=40.5263157894737, Blast_Score=130, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22209; Mature: 22209

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPLCVSPSAPSRVVVLASGTGSLLGSLIDASVGNYPARVVAVGVDRDCGATKIAKAAS
CCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCC
VPTFTVRLADPPGRDAWDAKITEAVASYKPDLVVLAGFMRILGPQFLARFFGRIVNTHPA
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
LLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILAQQSVPVLDGDDTAALHERIK
EEECCCCCCHHHHHHHHCEEECCCEEEEEECCCCCCCEEECCCCCEECCCCHHHHHHHHH
VIERRLLVDVVAVIATGGVTLVGRKATIGRKATIE
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQEPLCVSPSAPSRVVVLASGTGSLLGSLIDASVGNYPARVVAVGVDRDCGATKIAKAAS
CCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCC
VPTFTVRLADPPGRDAWDAKITEAVASYKPDLVVLAGFMRILGPQFLARFFGRIVNTHPA
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
LLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILAQQSVPVLDGDDTAALHERIK
EEECCCCCCHHHHHHHHCEEECCCEEEEEECCCCCCCEEECCCCCEECCCCHHHHHHHHH
VIERRLLVDVVAVIATGGVTLVGRKATIGRKATIE
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]