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The map label for this gene is rrmA [C]

Identifier: 221229439

GI number: 221229439

Start: 174493

End: 175305

Strand: Reverse

Name: rrmA [C]

Synonym: MLBr_00130

Alternate gene names: 221229439

Gene position: 175305-174493 (Counterclockwise)

Preceding gene: 221229446

Following gene: 221229438

Centisome position: 5.36

GC content: 55.97

Gene sequence:

>813_bases
ATGGCCTTCACGCGAATCCATAGCTTTCTCGCCAGCGCAGGCAATACCTCCATGTACAAGAGGGTTTGGAGGTTCTGGTA
CCCGCTAATGACGCATAAGCTCGGCACCGACGAAATTATGTTCATCAACTGGGCCTATGAAGAGGATCCACCGATGGCCT
TGCCACTGGAGGCATCTGACGAGCCCAACCGGGCCCACATCAACCTGTACCACCGCACAGCGACTCAGGTCAATCTGAGC
GGCAAACGAATACTGGAAGTTAGTTGCGGTCACGGCGGCGGAGCTTCGTACCTCACCCGTGCCTTGCACCCAGCCTCCTA
CACGGGGCTGGACTTGAATCCGGCAGGCATCAAGCTGTGCCAAAAGCGACACCAGCTGCCTGGGTTAGAATTTGTGCGAG
GGGACGCCGAAAACCTGCCCTTCGACAATGAATCCTTCGACGTGGTAATCAATATCGAAGCCTCGCACTGCTACCCGCAC
TTTCCACGCTTTCTTGCTGAGGTTGTTCGCGTGCTGCGCCCAGGAGGGCACTTAGCCTACGCCGACCTGCGGCCCAGTAA
TAAAGTCGGTGAATGGGAAGTTGACTTCGCCAATAGCCGATTGCAACAACTTTCACAGAGAGAAATCAATGCCGAGGTCC
TTCGTGGCATTGCGAGCAATTCACAAAAATCTCGAGACCTAGTCGATCGACATCTGCCGGCCTTCCTGCGCTTCGCTGGC
CGCGAGTTCATTGGTGTGCAAGGCACGCAGTTGTCCCGTTACCTGGAGGGCGGGGAGCTGTCATATCGGATGTACTCCTT
CGCCAAGGACTGA

Upstream 100 bases:

>100_bases
AGCCGCTTCCTGCGCCCAGGCATTCCAAACAACGCCTCGGCGGCAAACTCACGCCGCGTGCCATGGCAGCCACCCAAAGT
TTTTGCCATACTCCTTACCC

Downstream 100 bases:

>100_bases
GGCTGCGTCGATTGCAAGCACTCGGGTAGTGTCACCCGGATGAGCCCAGATAAGCGTGAGTTCGACATTGTTTTGTACGG
CGCTACAGGCTTCTCTGGCA

Product: hypothetical protein

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAFTRIHSFLASAGNTSMYKRVWRFWYPLMTHKLGTDEIMFINWAYEEDPPMALPLEASDEPNRAHINLYHRTATQVNLS
GKRILEVSCGHGGGASYLTRALHPASYTGLDLNPAGIKLCQKRHQLPGLEFVRGDAENLPFDNESFDVVINIEASHCYPH
FPRFLAEVVRVLRPGGHLAYADLRPSNKVGEWEVDFANSRLQQLSQREINAEVLRGIASNSQKSRDLVDRHLPAFLRFAG
REFIGVQGTQLSRYLEGGELSYRMYSFAKD

Sequences:

>Translated_270_residues
MAFTRIHSFLASAGNTSMYKRVWRFWYPLMTHKLGTDEIMFINWAYEEDPPMALPLEASDEPNRAHINLYHRTATQVNLS
GKRILEVSCGHGGGASYLTRALHPASYTGLDLNPAGIKLCQKRHQLPGLEFVRGDAENLPFDNESFDVVINIEASHCYPH
FPRFLAEVVRVLRPGGHLAYADLRPSNKVGEWEVDFANSRLQQLSQREINAEVLRGIASNSQKSRDLVDRHLPAFLRFAG
REFIGVQGTQLSRYLEGGELSYRMYSFAKD
>Mature_269_residues
AFTRIHSFLASAGNTSMYKRVWRFWYPLMTHKLGTDEIMFINWAYEEDPPMALPLEASDEPNRAHINLYHRTATQVNLSG
KRILEVSCGHGGGASYLTRALHPASYTGLDLNPAGIKLCQKRHQLPGLEFVRGDAENLPFDNESFDVVINIEASHCYPHF
PRFLAEVVRVLRPGGHLAYADLRPSNKVGEWEVDFANSRLQQLSQREINAEVLRGIASNSQKSRDLVDRHLPAFLRFAGR
EFIGVQGTQLSRYLEGGELSYRMYSFAKD

Specific function: Catalyzes the methylation of the lipid moiety of the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycoserosates to form phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL)

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase family

Homologues:

Organism=Caenorhabditis elegans, GI17540496, Length=182, Percent_Identity=29.6703296703297, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHMT_MYCLE (Q9CD86)

Other databases:

- EMBL:   AL583917
- PIR:   B86925
- RefSeq:   NP_301224.1
- ProteinModelPortal:   Q9CD86
- SMR:   Q9CD86
- EnsemblBacteria:   EBMYCT00000029467
- GeneID:   908432
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0130
- NMPDR:   fig|272631.1.peg.96
- Leproma:   ML0130
- GeneTree:   EBGT00050000014593
- HOGENOM:   HBG567260
- OMA:   RTATHVE
- ProtClustDB:   CLSK791223
- BioCyc:   MLEP272631:ML0130-MONOMER
- InterPro:   IPR013216

Pfam domain/function: PF08241 Methyltransf_11

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 30641; Mature: 30510

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTRIHSFLASAGNTSMYKRVWRFWYPLMTHKLGTDEIMFINWAYEEDPPMALPLEASD
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCC
EPNRAHINLYHRTATQVNLSGKRILEVSCGHGGGASYLTRALHPASYTGLDLNPAGIKLC
CCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHH
QKRHQLPGLEFVRGDAENLPFDNESFDVVINIEASHCYPHFPRFLAEVVRVLRPGGHLAY
HHHHCCCCCCEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
ADLRPSNKVGEWEVDFANSRLQQLSQREINAEVLRGIASNSQKSRDLVDRHLPAFLRFAG
EECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
REFIGVQGTQLSRYLEGGELSYRMYSFAKD
CHHCCCCCHHHHHHHCCCCEEEEEHHCCCC
>Mature Secondary Structure 
AFTRIHSFLASAGNTSMYKRVWRFWYPLMTHKLGTDEIMFINWAYEEDPPMALPLEASD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCC
EPNRAHINLYHRTATQVNLSGKRILEVSCGHGGGASYLTRALHPASYTGLDLNPAGIKLC
CCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHH
QKRHQLPGLEFVRGDAENLPFDNESFDVVINIEASHCYPHFPRFLAEVVRVLRPGGHLAY
HHHHCCCCCCEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEE
ADLRPSNKVGEWEVDFANSRLQQLSQREINAEVLRGIASNSQKSRDLVDRHLPAFLRFAG
EECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
REFIGVQGTQLSRYLEGGELSYRMYSFAKD
CHHCCCCCHHHHHHHCCCCEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002