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The map label for this gene is murF [H]

Identifier: 220918992

GI number: 220918992

Start: 4346018

End: 4347424

Strand: Direct

Name: murF [H]

Synonym: A2cp1_3907

Alternate gene names: 220918992

Gene position: 4346018-4347424 (Clockwise)

Preceding gene: 220918991

Following gene: 220918993

Centisome position: 86.41

GC content: 77.4

Gene sequence:

>1407_bases
ATGACCACTCCCCGCTCCACCCCGCAGTTCACGCCCGACGAGCTCGCGGGCGCCACGCGAGGCCGCTGGCTCGGCGCGCC
GCCGTCCGCGGTGGCGGGCGTCTCGACCGACACCCGCACGCTCTCCCCCGGGCAGCTCTTCGTGGCGCTGAAGGGCGAGC
GCTTCGACGCGCACGATCACCTGGAGGCCGCGGCCGCCGCCGGCGCCGCCGCGCTGGTGGTGTCGGAGGCCTGGGCCGCC
GCGCACCCCGCCCCGCCCCGCGCCGCGCTGGCGGTGGCCGACCCGCTCGCCGCCCTCGGCGCCATCGCCCGCCACCACCG
CTGCCGCTTCGGCATCCCGGTGATCGGGGTCACCGGCTCGAACGGCAAGACCACCACGCGCGAGATGATCGCGGCGATCC
TGGCCACCCGCGGCCCGGTGCTGAAGACCGAGGGCAACCTGAACAACGAGGTGGGCGTCCCGCTCACGCTGTTCGGCCTC
GAGGACGCGCACGCGACCGCCGTCATCGAGATGGGGATGAACCACCCCGGGGAGATCGCGCGCCTCGCCGCCATCGCCCA
GCCGCAGGTGGGCGTGGTGACGCTGGCGGCCCCCGCCCACCTGGAGGGGCTCGGCACGGTGGACGCGGTGGCCGACGCGA
AGGCCGAGCTGTACGAGGGGCTGCCCGCCGGCGGGATCGCGGTGGTGAACGCGGACGACGCGCGCATGCTGAAGCGCGGC
GTCGCCTCCGGGCGGCGCATGCTCACCTTCGCCTCCGGCCGCGGCCGCCGCGGCGACGTGGTGGTGCTCGAGGTCGTCTC
GCAGTCGGTGGAGGGGCTCCGCTTCGTCCTCGGCGTGGGCAACCGCGAGGTCCCGGTCCACATCCCCGGCCTGGTCGGCG
CGCACAACGCCCGCAACGCCGCGGCCGCCGCCTGCGCGGCCATCGCGGTGGGCTGCACCGACCGCGAGATCGCGCAGGGG
CTCGCCGCGGTGCGCCCGGTGGGCCGCCGCCTGCGCCTGGAGCGCCTCCCCTCCGGCGTCCAGCTGGTGGACGACTGCTA
CAACGCGAACCCGGCCTCGATGTCGGCGGCGCTGGACGTCCTGCGCGACCTCGCGGCCGCCCCCGGCGCCCGCGCGTTCG
CGGTGCTCGGGGACATGCTCGAGCTGGGCGCGTTCGAGGCGGAGGCGCACCGCGCGCTGGGCGCCGAGGCGGCCGCCGCG
GGCGTGGCGGTGCTGGCCGCGTTCGGGCCGCGCGCCCGCGCCACCGCCGAGGCGGCCGGCGCGGCCGGCCTCGCCGAGAC
CTTCCACACCGAGGACCTGGACCGCCTCGTCGCGTTCGTGCGCGAGCGGCTCCGTCCCACCGACGTCCTGCTCGTGAAGG
GGAGCCGCGGCATGAAGCTCGAGCGGCTCGTCGAGGCCCTGCGCTAG

Upstream 100 bases:

>100_bases
TGCTCGTCGCCGGCAAGGGGCACGAGGACTACCAGCAGGTGGGCGCCGAGAAGCGCCCCTTCTCCGACCGCGACGAGGCG
CGCAAGGCGCTCGGGATCGC

Downstream 100 bases:

>100_bases
ATGCTCTACCACCTCCTCTACCCGCTGGCCTACAAGCTCAGCCTCCTCAACGTCCTCCGCTACCCGTCGTTCCGCATCGT
GGCGGCGGGCCTCACCGCCA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTTPRSTPQFTPDELAGATRGRWLGAPPSAVAGVSTDTRTLSPGQLFVALKGERFDAHDHLEAAAAAGAAALVVSEAWAA
AHPAPPRAALAVADPLAALGAIARHHRCRFGIPVIGVTGSNGKTTTREMIAAILATRGPVLKTEGNLNNEVGVPLTLFGL
EDAHATAVIEMGMNHPGEIARLAAIAQPQVGVVTLAAPAHLEGLGTVDAVADAKAELYEGLPAGGIAVVNADDARMLKRG
VASGRRMLTFASGRGRRGDVVVLEVVSQSVEGLRFVLGVGNREVPVHIPGLVGAHNARNAAAAACAAIAVGCTDREIAQG
LAAVRPVGRRLRLERLPSGVQLVDDCYNANPASMSAALDVLRDLAAAPGARAFAVLGDMLELGAFEAEAHRALGAEAAAA
GVAVLAAFGPRARATAEAAGAAGLAETFHTEDLDRLVAFVRERLRPTDVLLVKGSRGMKLERLVEALR

Sequences:

>Translated_468_residues
MTTPRSTPQFTPDELAGATRGRWLGAPPSAVAGVSTDTRTLSPGQLFVALKGERFDAHDHLEAAAAAGAAALVVSEAWAA
AHPAPPRAALAVADPLAALGAIARHHRCRFGIPVIGVTGSNGKTTTREMIAAILATRGPVLKTEGNLNNEVGVPLTLFGL
EDAHATAVIEMGMNHPGEIARLAAIAQPQVGVVTLAAPAHLEGLGTVDAVADAKAELYEGLPAGGIAVVNADDARMLKRG
VASGRRMLTFASGRGRRGDVVVLEVVSQSVEGLRFVLGVGNREVPVHIPGLVGAHNARNAAAAACAAIAVGCTDREIAQG
LAAVRPVGRRLRLERLPSGVQLVDDCYNANPASMSAALDVLRDLAAAPGARAFAVLGDMLELGAFEAEAHRALGAEAAAA
GVAVLAAFGPRARATAEAAGAAGLAETFHTEDLDRLVAFVRERLRPTDVLLVKGSRGMKLERLVEALR
>Mature_467_residues
TTPRSTPQFTPDELAGATRGRWLGAPPSAVAGVSTDTRTLSPGQLFVALKGERFDAHDHLEAAAAAGAAALVVSEAWAAA
HPAPPRAALAVADPLAALGAIARHHRCRFGIPVIGVTGSNGKTTTREMIAAILATRGPVLKTEGNLNNEVGVPLTLFGLE
DAHATAVIEMGMNHPGEIARLAAIAQPQVGVVTLAAPAHLEGLGTVDAVADAKAELYEGLPAGGIAVVNADDARMLKRGV
ASGRRMLTFASGRGRRGDVVVLEVVSQSVEGLRFVLGVGNREVPVHIPGLVGAHNARNAAAAACAAIAVGCTDREIAQGL
AAVRPVGRRLRLERLPSGVQLVDDCYNANPASMSAALDVLRDLAAAPGARAFAVLGDMLELGAFEAEAHRALGAEAAAAG
VAVLAAFGPRARATAEAAGAAGLAETFHTEDLDRLVAFVRERLRPTDVLLVKGSRGMKLERLVEALR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=437, Percent_Identity=41.6475972540046, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48049; Mature: 47917

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRSTPQFTPDELAGATRGRWLGAPPSAVAGVSTDTRTLSPGQLFVALKGERFDAHDH
CCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHH
LEAAAAAGAAALVVSEAWAAAHPAPPRAALAVADPLAALGAIARHHRCRFGIPVIGVTGS
HHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEEECC
NGKTTTREMIAAILATRGPVLKTEGNLNNEVGVPLTLFGLEDAHATAVIEMGMNHPGEIA
CCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCHHEEEEEECCCCCHHHH
RLAAIAQPQVGVVTLAAPAHLEGLGTVDAVADAKAELYEGLPAGGIAVVNADDARMLKRG
HHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
VASGRRMLTFASGRGRRGDVVVLEVVSQSVEGLRFVLGVGNREVPVHIPGLVGAHNARNA
HHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHEEEEEECCCCCCCEECCCCCCCCCCCHH
AAAACAAIAVGCTDREIAQGLAAVRPVGRRLRLERLPSGVQLVDDCYNANPASMSAALDV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
LRDLAAAPGARAFAVLGDMLELGAFEAEAHRALGAEAAAAGVAVLAAFGPRARATAEAAG
HHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHC
AAGLAETFHTEDLDRLVAFVRERLRPTDVLLVKGSRGMKLERLVEALR
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHC
>Mature Secondary Structure 
TTPRSTPQFTPDELAGATRGRWLGAPPSAVAGVSTDTRTLSPGQLFVALKGERFDAHDH
CCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHH
LEAAAAAGAAALVVSEAWAAAHPAPPRAALAVADPLAALGAIARHHRCRFGIPVIGVTGS
HHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEEECC
NGKTTTREMIAAILATRGPVLKTEGNLNNEVGVPLTLFGLEDAHATAVIEMGMNHPGEIA
CCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCHHEEEEEECCCCCHHHH
RLAAIAQPQVGVVTLAAPAHLEGLGTVDAVADAKAELYEGLPAGGIAVVNADDARMLKRG
HHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHH
VASGRRMLTFASGRGRRGDVVVLEVVSQSVEGLRFVLGVGNREVPVHIPGLVGAHNARNA
HHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHEEEEEECCCCCCCEECCCCCCCCCCCHH
AAAACAAIAVGCTDREIAQGLAAVRPVGRRLRLERLPSGVQLVDDCYNANPASMSAALDV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
LRDLAAAPGARAFAVLGDMLELGAFEAEAHRALGAEAAAAGVAVLAAFGPRARATAEAAG
HHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHC
AAGLAETFHTEDLDRLVAFVRERLRPTDVLLVKGSRGMKLERLVEALR
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]