Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 220918991

GI number: 220918991

Start: 4344531

End: 4346021

Strand: Direct

Name: murE [H]

Synonym: A2cp1_3906

Alternate gene names: 220918991

Gene position: 4344531-4346021 (Clockwise)

Preceding gene: 220918990

Following gene: 220918992

Centisome position: 86.38

GC content: 77.4

Gene sequence:

>1491_bases
ATGAGGCTCTCCACCGTCATCGCCGGCAGCGGCGCCCGCGGCGATCTCGCCGGCGATCCCGAGATCACCCGCGTCACCGG
CGACTCGCGGGAGGTGATCCCGGGCGCGGTGTTCTTCGCGCTCCCCGGCGTCCAGCACGACGGCCACGCCTTCGCCGCCG
AGGCGGCGCGACGGGGCGCCATCGCCGTGGTCGCCGAGCGGCCGGTCGAGTGCGCGCCCGCGCGGCTGCTGCTCGCCCCG
TCCGCGCGCAGGGCCATGGCGCTCGCCGCCGCCAGCTTCCACGGCCGGCCGGCCGAGCGCCTCCGCATGGCCGCGGTCAC
CGGCACGAACGGGAAGACGACCGTCACCTACCTGGTGGAGGCGTGCGCGCGCGCCGCCGCGGTGCCGGTGGGCGTGCTCG
GGACGATCTCGTTCCGCTTCCCCGGCGCGGACCGGCCCGCCAGCCACACCACTCCGGAGTCCACCGCCATCCAGGCGGTG
CTGGCGGAGATGGTCGCGGCCGGCGCGAGGGACGCGGTGATGGAGGTCTCGTCGCACGCGCTCGCGCAGGAGCGGGTGGC
CGGGATCCGCTTCCAGGCGGCCGGCTTCACGAACCTCACGCGCGACCACCTCGACTACCACCGCGACATGGACGCGTACT
TCGCGGCGAAGCGCCGGCTGTTCACCGAGCACCTCGCGCCCGGCGGCACCGCGGTGATCAACGCGCGCGATCCCTGGGGC
GCGCGCCTGGCCGACCAGCTCGGGCCGGGGCCGCGCGTCTGGCGCTACGGCGTCCGGGCCGGCGACGCGCTGCGCGCGCT
CGAGGTGCGGACCGGCCTCGCCGGCACCAGCGCGACGTTCGAGACGCCGGCCGGCGCCATCGCGATCCGCTCCCCGCTCG
TCGGCCAGCACAACCTCGAGAACCTGCTCTGCGCCGCGGGCCTCGCGCTCGGCCTGGGCCTGCCGCCGGAGGCGGTCGCC
CGCGGCCTCGCCGCCTCGCACGGCGCGCCGGGCCGGCTGGAGCGGATCGACGGCCGCGGCCTGTCGGTCTTCGTGGACTA
CGCCCACACCGACGACGCGCTGGCGCGCATGATCGCGGCGCTGCGCGGGCTCTCGCCGCGGCGGCTGATCTGCGTGTTCG
GCTGCGGCGGCGACCGGGACCGCGGCAAGCGCCCGCTCATGGGCGCGGCGGCCGCGGCCGCGGACCTCGCCATCGTCACC
AGCGACAACCCGCGCACCGAGGACCCGCTCGCCATCATCGCCGACGTGCTGCCCGGCCTGGACCGCGCCGGGAAGCCCTC
CCTCGGCGCCGCCCGCGCGCGCGCCGGCGAGGACGGCTACCTGGTCGAGCCGGACCGTCGCGCCGCCATCGCGCTCGCGG
TGGAGTGCGCGCGCGAGGGCGACGCGGTGCTCGTCGCCGGCAAGGGGCACGAGGACTACCAGCAGGTGGGCGCCGAGAAG
CGCCCCTTCTCCGACCGCGACGAGGCGCGCAAGGCGCTCGGGATCGCATGA

Upstream 100 bases:

>100_bases
CCCGTGTCCCGCCGGACACGCCCGCGCGCAAGCGGCCGGACCCGCGGAGGAAAACGAGGCGGACGGCCGTTTGCATGGTA
TCTCCCGCTGGTAGCGAACA

Downstream 100 bases:

>100_bases
CCACTCCCCGCTCCACCCCGCAGTTCACGCCCGACGAGCTCGCGGGCGCCACGCGAGGCCGCTGGCTCGGCGCGCCGCCG
TCCGCGGTGGCGGGCGTCTC

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MRLSTVIAGSGARGDLAGDPEITRVTGDSREVIPGAVFFALPGVQHDGHAFAAEAARRGAIAVVAERPVECAPARLLLAP
SARRAMALAAASFHGRPAERLRMAAVTGTNGKTTVTYLVEACARAAAVPVGVLGTISFRFPGADRPASHTTPESTAIQAV
LAEMVAAGARDAVMEVSSHALAQERVAGIRFQAAGFTNLTRDHLDYHRDMDAYFAAKRRLFTEHLAPGGTAVINARDPWG
ARLADQLGPGPRVWRYGVRAGDALRALEVRTGLAGTSATFETPAGAIAIRSPLVGQHNLENLLCAAGLALGLGLPPEAVA
RGLAASHGAPGRLERIDGRGLSVFVDYAHTDDALARMIAALRGLSPRRLICVFGCGGDRDRGKRPLMGAAAAAADLAIVT
SDNPRTEDPLAIIADVLPGLDRAGKPSLGAARARAGEDGYLVEPDRRAAIALAVECAREGDAVLVAGKGHEDYQQVGAEK
RPFSDRDEARKALGIA

Sequences:

>Translated_496_residues
MRLSTVIAGSGARGDLAGDPEITRVTGDSREVIPGAVFFALPGVQHDGHAFAAEAARRGAIAVVAERPVECAPARLLLAP
SARRAMALAAASFHGRPAERLRMAAVTGTNGKTTVTYLVEACARAAAVPVGVLGTISFRFPGADRPASHTTPESTAIQAV
LAEMVAAGARDAVMEVSSHALAQERVAGIRFQAAGFTNLTRDHLDYHRDMDAYFAAKRRLFTEHLAPGGTAVINARDPWG
ARLADQLGPGPRVWRYGVRAGDALRALEVRTGLAGTSATFETPAGAIAIRSPLVGQHNLENLLCAAGLALGLGLPPEAVA
RGLAASHGAPGRLERIDGRGLSVFVDYAHTDDALARMIAALRGLSPRRLICVFGCGGDRDRGKRPLMGAAAAAADLAIVT
SDNPRTEDPLAIIADVLPGLDRAGKPSLGAARARAGEDGYLVEPDRRAAIALAVECAREGDAVLVAGKGHEDYQQVGAEK
RPFSDRDEARKALGIA
>Mature_496_residues
MRLSTVIAGSGARGDLAGDPEITRVTGDSREVIPGAVFFALPGVQHDGHAFAAEAARRGAIAVVAERPVECAPARLLLAP
SARRAMALAAASFHGRPAERLRMAAVTGTNGKTTVTYLVEACARAAAVPVGVLGTISFRFPGADRPASHTTPESTAIQAV
LAEMVAAGARDAVMEVSSHALAQERVAGIRFQAAGFTNLTRDHLDYHRDMDAYFAAKRRLFTEHLAPGGTAVINARDPWG
ARLADQLGPGPRVWRYGVRAGDALRALEVRTGLAGTSATFETPAGAIAIRSPLVGQHNLENLLCAAGLALGLGLPPEAVA
RGLAASHGAPGRLERIDGRGLSVFVDYAHTDDALARMIAALRGLSPRRLICVFGCGGDRDRGKRPLMGAAAAAADLAIVT
SDNPRTEDPLAIIADVLPGLDRAGKPSLGAARARAGEDGYLVEPDRRAAIALAVECAREGDAVLVAGKGHEDYQQVGAEK
RPFSDRDEARKALGIA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=490, Percent_Identity=40.6122448979592, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1786274, Length=359, Percent_Identity=28.9693593314763, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51630; Mature: 51630

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSTVIAGSGARGDLAGDPEITRVTGDSREVIPGAVFFALPGVQHDGHAFAAEAARRGA
CCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCC
IAVVAERPVECAPARLLLAPSARRAMALAAASFHGRPAERLRMAAVTGTNGKTTVTYLVE
EEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCHHHHHHHH
ACARAAAVPVGVLGTISFRFPGADRPASHTTPESTAIQAVLAEMVAAGARDAVMEVSSHA
HHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAQERVAGIRFQAAGFTNLTRDHLDYHRDMDAYFAAKRRLFTEHLAPGGTAVINARDPWG
HHHHHHHCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCH
ARLADQLGPGPRVWRYGVRAGDALRALEVRTGLAGTSATFETPAGAIAIRSPLVGQHNLE
HHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHH
NLLCAAGLALGLGLPPEAVARGLAASHGAPGRLERIDGRGLSVFVDYAHTDDALARMIAA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCHHHHHHHHHH
LRGLSPRRLICVFGCGGDRDRGKRPLMGAAAAAADLAIVTSDNPRTEDPLAIIADVLPGL
HHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCC
DRAGKPSLGAARARAGEDGYLVEPDRRAAIALAVECAREGDAVLVAGKGHEDYQQVGAEK
CCCCCCCCCHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHCCCCC
RPFSDRDEARKALGIA
CCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRLSTVIAGSGARGDLAGDPEITRVTGDSREVIPGAVFFALPGVQHDGHAFAAEAARRGA
CCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCC
IAVVAERPVECAPARLLLAPSARRAMALAAASFHGRPAERLRMAAVTGTNGKTTVTYLVE
EEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCHHHHHHHH
ACARAAAVPVGVLGTISFRFPGADRPASHTTPESTAIQAVLAEMVAAGARDAVMEVSSHA
HHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAQERVAGIRFQAAGFTNLTRDHLDYHRDMDAYFAAKRRLFTEHLAPGGTAVINARDPWG
HHHHHHHCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCH
ARLADQLGPGPRVWRYGVRAGDALRALEVRTGLAGTSATFETPAGAIAIRSPLVGQHNLE
HHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCHH
NLLCAAGLALGLGLPPEAVARGLAASHGAPGRLERIDGRGLSVFVDYAHTDDALARMIAA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCHHHHHHHHHH
LRGLSPRRLICVFGCGGDRDRGKRPLMGAAAAAADLAIVTSDNPRTEDPLAIIADVLPGL
HHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCC
DRAGKPSLGAARARAGEDGYLVEPDRRAAIALAVECAREGDAVLVAGKGHEDYQQVGAEK
CCCCCCCCCHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHCCCCC
RPFSDRDEARKALGIA
CCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA