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The map label for this gene is fdoH [H]

Identifier: 220918980

GI number: 220918980

Start: 4334281

End: 4335156

Strand: Direct

Name: fdoH [H]

Synonym: A2cp1_3895

Alternate gene names: 220918980

Gene position: 4334281-4335156 (Clockwise)

Preceding gene: 220918979

Following gene: 220918981

Centisome position: 86.18

GC content: 68.95

Gene sequence:

>876_bases
ATGGCGTTCCAGTCGCTCGACGTCATCAACCGGTCCGCCTCCACCAGCACGCCGCCGCAGGCGCGCGGCGCGCTGGAGGT
GGCGAAGCTCATCGACATCTCCAAGTGCATCGGCTGCAAGGCCTGCCAGTCGGCGTGCATGGAGTGGAACGACCTCCGCG
ACGAGGTCGGGGTGAACCCCGGGCACTACGACAACCCGGCCGACCTCACCGCGCAGTCGTGGACGGTGATGCGCTTCTAC
GAGGAGGAGCTCCCCGCGGACAGGGGGCTCGCCTGGCTCATCGTGAAGGACGGCTGCCTGCACTGCGCCGAGCCCGGGTG
CCTGAAGGCGTGCCCGGCGCCGGGCGCGATCGTGCAGTACGCGAACGGGATCGTGGACTTCCAGCAGGACCAGTGCATCG
GCTGCGGCTACTGCCAGACCGGCTGCCCGTTCAACATCCCGCGCTACTCGATGAAGGACCAGAAGGCGTACAAGTGCACG
CTGTGCTCCGACCGCGTCTCGGTCGGGCTCGAGCCGGCGTGCGTCAAGACCTGCCCCACCGGCGCGCTCGCGTTCGGCAC
CAAGACCGACATGAAGGACCTCGCCGGCGAGCGCCTGGTCGAGCTGAAGGCGCGCGGCTTCGAGAAGGCGGCGCTCTACG
ATCCGTCCGGCGTGGGCGGCACGCACGTGCTGTTCGTGCTCCCGCACGGCGATCCGGAGCTGTACCGCCTGCCCAAGGAC
CCGCGGGTGTCGCCGCTGGTGGCGCTGTGGCGCAGCGGCGTCGCCAAGACGCTCGGCGTCCTCACCATGGTCTCGGTCGT
GGTGGCCGGGATCTTCCACTACATGAAGGTCGGACCGGTCGAGGTCGACGAGGACCAGAAGGAGAACGGGTCATGA

Upstream 100 bases:

>100_bases
CACCCCCTTCGTCGGCGACGCGAACACCCAGACGCCCGAGTTCAAGGCGTTCCTGGTGGACGTGGAGAAGGCCGCGGCGG
GGCAGGTCTAGGAGGCGGCC

Downstream 100 bases:

>100_bases
TGCCTGCGATGCGGATGCCGCCGGGGAAGGAGGTCGTCCCCCGCTACTCGACCGGCGAGCGCCTCACGCACTGGGCGGTG
GCGGTCGCGTACGTGGCCCT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAFQSLDVINRSASTSTPPQARGALEVAKLIDISKCIGCKACQSACMEWNDLRDEVGVNPGHYDNPADLTAQSWTVMRFY
EEELPADRGLAWLIVKDGCLHCAEPGCLKACPAPGAIVQYANGIVDFQQDQCIGCGYCQTGCPFNIPRYSMKDQKAYKCT
LCSDRVSVGLEPACVKTCPTGALAFGTKTDMKDLAGERLVELKARGFEKAALYDPSGVGGTHVLFVLPHGDPELYRLPKD
PRVSPLVALWRSGVAKTLGVLTMVSVVVAGIFHYMKVGPVEVDEDQKENGS

Sequences:

>Translated_291_residues
MAFQSLDVINRSASTSTPPQARGALEVAKLIDISKCIGCKACQSACMEWNDLRDEVGVNPGHYDNPADLTAQSWTVMRFY
EEELPADRGLAWLIVKDGCLHCAEPGCLKACPAPGAIVQYANGIVDFQQDQCIGCGYCQTGCPFNIPRYSMKDQKAYKCT
LCSDRVSVGLEPACVKTCPTGALAFGTKTDMKDLAGERLVELKARGFEKAALYDPSGVGGTHVLFVLPHGDPELYRLPKD
PRVSPLVALWRSGVAKTLGVLTMVSVVVAGIFHYMKVGPVEVDEDQKENGS
>Mature_290_residues
AFQSLDVINRSASTSTPPQARGALEVAKLIDISKCIGCKACQSACMEWNDLRDEVGVNPGHYDNPADLTAQSWTVMRFYE
EELPADRGLAWLIVKDGCLHCAEPGCLKACPAPGAIVQYANGIVDFQQDQCIGCGYCQTGCPFNIPRYSMKDQKAYKCTL
CSDRVSVGLEPACVKTCPTGALAFGTKTDMKDLAGERLVELKARGFEKAALYDPSGVGGTHVLFVLPHGDPELYRLPKDP
RVSPLVALWRSGVAKTLGVLTMVSVVVAGIFHYMKVGPVEVDEDQKENGS

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=292, Percent_Identity=62.3287671232877, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787749, Length=295, Percent_Identity=61.6949152542373, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789370, Length=299, Percent_Identity=27.0903010033445, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787122, Length=168, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787872, Length=167, Percent_Identity=32.9341317365269, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367345, Length=161, Percent_Identity=32.9192546583851, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI226510944, Length=165, Percent_Identity=29.6969696969697, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788811, Length=176, Percent_Identity=27.8409090909091, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787478, Length=80, Percent_Identity=36.25, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787740, Length=112, Percent_Identity=31.25, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082179, Length=175, Percent_Identity=28, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787963, Length=150, Percent_Identity=26, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=29.4478527607362, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 31454; Mature: 31323

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQSLDVINRSASTSTPPQARGALEVAKLIDISKCIGCKACQSACMEWNDLRDEVGVNP
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHCCCC
GHYDNPADLTAQSWTVMRFYEEELPADRGLAWLIVKDGCLHCAEPGCLKACPAPGAIVQY
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
ANGIVDFQQDQCIGCGYCQTGCPFNIPRYSMKDQKAYKCTLCSDRVSVGLEPACVKTCPT
HCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCHHHHHHCCC
GALAFGTKTDMKDLAGERLVELKARGFEKAALYDPSGVGGTHVLFVLPHGDPELYRLPKD
CCEECCCCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCEEEEEEEECCCCCEEECCCC
PRVSPLVALWRSGVAKTLGVLTMVSVVVAGIFHYMKVGPVEVDEDQKENGS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
AFQSLDVINRSASTSTPPQARGALEVAKLIDISKCIGCKACQSACMEWNDLRDEVGVNP
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHCCCC
GHYDNPADLTAQSWTVMRFYEEELPADRGLAWLIVKDGCLHCAEPGCLKACPAPGAIVQY
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
ANGIVDFQQDQCIGCGYCQTGCPFNIPRYSMKDQKAYKCTLCSDRVSVGLEPACVKTCPT
HCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCHHHHHHCCC
GALAFGTKTDMKDLAGERLVELKARGFEKAALYDPSGVGGTHVLFVLPHGDPELYRLPKD
CCEECCCCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCEEEEEEEECCCCCEEECCCC
PRVSPLVALWRSGVAKTLGVLTMVSVVVAGIFHYMKVGPVEVDEDQKENGS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]