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The map label for this gene is fdoI [H]

Identifier: 220918981

GI number: 220918981

Start: 4335153

End: 4335803

Strand: Direct

Name: fdoI [H]

Synonym: A2cp1_3896

Alternate gene names: 220918981

Gene position: 4335153-4335803 (Clockwise)

Preceding gene: 220918980

Following gene: 220918982

Centisome position: 86.2

GC content: 68.05

Gene sequence:

>651_bases
ATGATGCCTGCGATGCGGATGCCGCCGGGGAAGGAGGTCGTCCCCCGCTACTCGACCGGCGAGCGCCTCACGCACTGGGC
GGTGGCGGTCGCGTACGTGGCCCTGTTCCTGTCCGGCCTCGCCATGTTCCACCCGTTCTTCTACTGGACCAGCGCGCTGT
TCGGCAGCGCCGCGTTCATGCGCGTGCTCCACCCGTTCCTCGGCGTGGCGCTGTTCGTGCTGTTCTACGCGTACGCGCTG
CGCCTCTGGCGCGACAACATCCTGAGCGACAGCGACCGGAAGTGGATGGGCCAGATGGTCGCGTACATGAACAAGGCGGC
CGAGCCCTACGTGGACGGCAAGTACAACGCCGGCCAGAAGCTCATGTACTGGTCGATGATCGTGGTGATCGCCGGCCTGT
TCCTCACCGGCATCGTGCTGTGGCGCCCCTACTTCGCGCCCTCCTTCTCGCTCGTCGCGCGCCGCTTCGCGGCGGTGATC
CACGCCGGGCTCGCGTTCGTGATGTTCGTGGGCATCGGCATCCACGTGTACGCGGCCTACTGGACCAAGGGCTCGATGCG
CGCCATGACCCGGGGCACCGTCTCCGGCGCGTGGGCGCGCTTCCATCACCCGGGCTGGTACGCCCGGATGACCGGCAAGG
AGCGGCCGTGA

Upstream 100 bases:

>100_bases
TCGGCGTCCTCACCATGGTCTCGGTCGTGGTGGCCGGGATCTTCCACTACATGAAGGTCGGACCGGTCGAGGTCGACGAG
GACCAGAAGGAGAACGGGTC

Downstream 100 bases:

>100_bases
CGCGGCGGCGCGGGGCGGCGATCTCGGGGGAGGCCGCGTGAGCACGCTCGTGCAGCTCCCCGGGGTCGAGATCCCGTACC
TGCGCCTGCCCGAGCCGGGC

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MMPAMRMPPGKEVVPRYSTGERLTHWAVAVAYVALFLSGLAMFHPFFYWTSALFGSAAFMRVLHPFLGVALFVLFYAYAL
RLWRDNILSDSDRKWMGQMVAYMNKAAEPYVDGKYNAGQKLMYWSMIVVIAGLFLTGIVLWRPYFAPSFSLVARRFAAVI
HAGLAFVMFVGIGIHVYAAYWTKGSMRAMTRGTVSGAWARFHHPGWYARMTGKERP

Sequences:

>Translated_216_residues
MMPAMRMPPGKEVVPRYSTGERLTHWAVAVAYVALFLSGLAMFHPFFYWTSALFGSAAFMRVLHPFLGVALFVLFYAYAL
RLWRDNILSDSDRKWMGQMVAYMNKAAEPYVDGKYNAGQKLMYWSMIVVIAGLFLTGIVLWRPYFAPSFSLVARRFAAVI
HAGLAFVMFVGIGIHVYAAYWTKGSMRAMTRGTVSGAWARFHHPGWYARMTGKERP
>Mature_216_residues
MMPAMRMPPGKEVVPRYSTGERLTHWAVAVAYVALFLSGLAMFHPFFYWTSALFGSAAFMRVLHPFLGVALFVLFYAYAL
RLWRDNILSDSDRKWMGQMVAYMNKAAEPYVDGKYNAGQKLMYWSMIVVIAGLFLTGIVLWRPYFAPSFSLVARRFAAVI
HAGLAFVMFVGIGIHVYAAYWTKGSMRAMTRGTVSGAWARFHHPGWYARMTGKERP

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=207, Percent_Identity=36.7149758454106, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1787750, Length=204, Percent_Identity=37.2549019607843, Blast_Score=150, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24549; Mature: 24549

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPAMRMPPGKEVVPRYSTGERLTHWAVAVAYVALFLSGLAMFHPFFYWTSALFGSAAFM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLHPFLGVALFVLFYAYALRLWRDNILSDSDRKWMGQMVAYMNKAAEPYVDGKYNAGQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
LMYWSMIVVIAGLFLTGIVLWRPYFAPSFSLVARRFAAVIHAGLAFVMFVGIGIHVYAAY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTKGSMRAMTRGTVSGAWARFHHPGWYARMTGKERP
HCCCCHHHHHHCCHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MMPAMRMPPGKEVVPRYSTGERLTHWAVAVAYVALFLSGLAMFHPFFYWTSALFGSAAFM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLHPFLGVALFVLFYAYALRLWRDNILSDSDRKWMGQMVAYMNKAAEPYVDGKYNAGQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
LMYWSMIVVIAGLFLTGIVLWRPYFAPSFSLVARRFAAVIHAGLAFVMFVGIGIHVYAAY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTKGSMRAMTRGTVSGAWARFHHPGWYARMTGKERP
HCCCCHHHHHHCCHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]