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The map label for this gene is bioH [H]

Identifier: 220918958

GI number: 220918958

Start: 4311178

End: 4311993

Strand: Direct

Name: bioH [H]

Synonym: A2cp1_3873

Alternate gene names: 220918958

Gene position: 4311178-4311993 (Clockwise)

Preceding gene: 220918957

Following gene: 220918959

Centisome position: 85.72

GC content: 80.88

Gene sequence:

>816_bases
ATGCCCCTCCTCGTCACCGACAGCGGCGTGCGCCTCGCGTACGCGGATCGCGGCGCCGGCGCGCCGCTGGTGCTGGTCCA
CGGCTGGTCGCTCTCCTCCGCCGCGTTCGACGACCTCGCCGTGCGGCTCCCCGGTCGCCGCCTGGTGACGCCCGACCTGC
GCGGCCACGGCGGCTCCGGCGCGGCGCCCTTCGCGCTGGAGGACCTGGGCCGCGACCTCGCGCTCCTGCTGGATCGGCTC
GACCTCCGCGGCGCGGTGCTGGCCGGCTGGTCGCTCGGCGCGCAGGCCGCGCTCGCCGCGCTCCCGGCGGTGCGCGCACG
GCTCGCGGGGCTGGTGCTGATCTCCGGGACCCCGCGCTTCACGCTCTGTGACGGATGGCCGCACGGGCTGCCCGCGCAGG
CGCTGGAGGTGCTGGCGCACCGGGTCCGCCGCGAACCGGCGCGCGCGGCGGCCCGCTTCTTCGACGGCATGTTCGCGCCC
GGCGAGCTCGACCCGGCCGCGCGCGCGCGCACCGGGGCGCTCCGCGCCGCGATCCCCTCGCCCGGCTCCGCCGCGGCGCT
CGCCGGGCTGGACGTGCTCGCCGCCGCGGATCTCCGGCCCACGCTCGCCGGCGTCGGGGTGCCCGCGCTGGTGATCCACG
GCACGGCCGATCCGATCTGCCCGCCCGGCGCCGGCAGGGCGCTCGCGGCGGGCATCCCCGGCGCGCGCCTCGCCCTGCTC
CCCGGCGCCGGCCACGCGCCGCACCTCACCCGCCCGGACGAGGTCGCCGCGCTGCTCCAGGCGTTCCCCGCCGGGGCCGG
GGCGGTGGCCGCGTGA

Upstream 100 bases:

>100_bases
GCCCCCCTTCCGCCGGTCCCCGCCGCCTCGTCAACCGTGACCTCCGGGTACGGTTGACGAGGCGGTCCTCGCTGGTATCC
TGCGCCACTCCGGCCCAGCG

Downstream 100 bases:

>100_bases
ACCGCGTCGACAAGCGCCGGGTCCAGGCCGCGTTCTCGCGCTCCGCCGCGGCGTACGACGCGCGCGCCTCGGTGCAGCGC
GAGGTGCAGGACCGCGTGCT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPLLVTDSGVRLAYADRGAGAPLVLVHGWSLSSAAFDDLAVRLPGRRLVTPDLRGHGGSGAAPFALEDLGRDLALLLDRL
DLRGAVLAGWSLGAQAALAALPAVRARLAGLVLISGTPRFTLCDGWPHGLPAQALEVLAHRVRREPARAAARFFDGMFAP
GELDPAARARTGALRAAIPSPGSAAALAGLDVLAAADLRPTLAGVGVPALVIHGTADPICPPGAGRALAAGIPGARLALL
PGAGHAPHLTRPDEVAALLQAFPAGAGAVAA

Sequences:

>Translated_271_residues
MPLLVTDSGVRLAYADRGAGAPLVLVHGWSLSSAAFDDLAVRLPGRRLVTPDLRGHGGSGAAPFALEDLGRDLALLLDRL
DLRGAVLAGWSLGAQAALAALPAVRARLAGLVLISGTPRFTLCDGWPHGLPAQALEVLAHRVRREPARAAARFFDGMFAP
GELDPAARARTGALRAAIPSPGSAAALAGLDVLAAADLRPTLAGVGVPALVIHGTADPICPPGAGRALAAGIPGARLALL
PGAGHAPHLTRPDEVAALLQAFPAGAGAVAA
>Mature_270_residues
PLLVTDSGVRLAYADRGAGAPLVLVHGWSLSSAAFDDLAVRLPGRRLVTPDLRGHGGSGAAPFALEDLGRDLALLLDRLD
LRGAVLAGWSLGAQAALAALPAVRARLAGLVLISGTPRFTLCDGWPHGLPAQALEVLAHRVRREPARAAARFFDGMFAPG
ELDPAARARTGALRAAIPSPGSAAALAGLDVLAAADLRPTLAGVGVPALVIHGTADPICPPGAGRALAAGIPGARLALLP
GAGHAPHLTRPDEVAALLQAFPAGAGAVAA

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=258, Percent_Identity=28.6821705426357, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 27191; Mature: 27060

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLVTDSGVRLAYADRGAGAPLVLVHGWSLSSAAFDDLAVRLPGRRLVTPDLRGHGGSG
CCEEEECCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHEECCCCEEECCCCCCCCCCC
AAPFALEDLGRDLALLLDRLDLRGAVLAGWSLGAQAALAALPAVRARLAGLVLISGTPRF
CCCHHHHHHCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCEEEECCCCCE
TLCDGWPHGLPAQALEVLAHRVRREPARAAARFFDGMFAPGELDPAARARTGALRAAIPS
EEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEECCC
PGSAAALAGLDVLAAADLRPTLAGVGVPALVIHGTADPICPPGAGRALAAGIPGARLALL
CCCHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCCEEEEE
PGAGHAPHLTRPDEVAALLQAFPAGAGAVAA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLLVTDSGVRLAYADRGAGAPLVLVHGWSLSSAAFDDLAVRLPGRRLVTPDLRGHGGSG
CEEEECCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHEECCCCEEECCCCCCCCCCC
AAPFALEDLGRDLALLLDRLDLRGAVLAGWSLGAQAALAALPAVRARLAGLVLISGTPRF
CCCHHHHHHCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCEEEECCCCCE
TLCDGWPHGLPAQALEVLAHRVRREPARAAARFFDGMFAPGELDPAARARTGALRAAIPS
EEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEECCC
PGSAAALAGLDVLAAADLRPTLAGVGVPALVIHGTADPICPPGAGRALAAGIPGARLALL
CCCHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCCEEEEE
PGAGHAPHLTRPDEVAALLQAFPAGAGAVAA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]