Click here to switch to the map view.

The map label for this gene is hycG [H]

Identifier: 220918887

GI number: 220918887

Start: 4223491

End: 4224270

Strand: Direct

Name: hycG [H]

Synonym: A2cp1_3801

Alternate gene names: 220918887

Gene position: 4223491-4224270 (Clockwise)

Preceding gene: 220918886

Following gene: 220918890

Centisome position: 83.98

GC content: 76.28

Gene sequence:

>780_bases
GTGATCGACATCCTGAAGTTCCGGCTCGGCTACGGGAACCAGACCGTGGCCTTCCCCGACGGCCCGCCCCGCTTCCCGGC
GCGCTTCCGGGGGCGGCCGGTGGTGGATCCGTCGCGCTGCGAGGCCGGCTGCGCCGGCTGCGCCGGCTGCGCCGAGGCGT
GCCCCACCGAGGCGATCCTCGACCCCGGCACCGCGACGATGCGGGTGGACCTGGGGCGCTGCACGTTCTGCGGCGACTGC
GTCACCGCCTGCCCGCCGGGCGCGATCCGCTTCACCGGCGACCACCGCCTCGCCGCCCGCACCCGCGAGGCGCTGGTGGT
GTCGCGCGACGCGCCGGTGCTCGCGGAGGCGCTCGACGGCGAGCTGCGGCGGGTGCTGGGCCGCTCGCTCAAGCTCCGGC
AGGTCTCGGCGGGCGGCTGCAGCGGCTGCGAGGCGGAGCTGAACGCCTCCGGCAACATCCAGTTCGACGTGGGCCGCTTC
GGCGTGCAGTTCGTCGCCTCGCCCCGCCACGCGGACGGCCTCGTGATCACCGGGCCCGTCACCGAGAACATGCGCCTCGC
GCTGCGCCAGACCTGGGAGGCGGTGCCCGCGCCGCGGATCGTGATCGCGGTGGGCGCCTGCGCCATCTCCGGCGGCCCGT
TCGCGGGCAGCCCGGTGACCTGCGGCGGCGCCGCGGCCGCGCTCGACGGGATCCCGGTGGACCTCTACGTCCCCGGGTGC
CCGCCGCACCCGATCACGTTCCTCGACGGGATCCTGCGGCTGCTCGGGCGCCTGAAGTAG

Upstream 100 bases:

>100_bases
ACAACTGGTTCGGGCTCGCCATGGCGCTCCGCGACGGCCAGATCTCCGACTTCCCGCTCTGCAACAAGAGCTTCAACCTC
TCCTACGCGGGGCACGACCT

Downstream 100 bases:

>100_bases
GCCGGCCGCCGCCCGCGTCAGCCCGTCCAGCCGGTCGGCGCCGGGCGCGCGTCCTCCCGCGGCGCGTGCGGCGGGAGCAG
GAGCGGCTCCTCGGGCATCG

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIDILKFRLGYGNQTVAFPDGPPRFPARFRGRPVVDPSRCEAGCAGCAGCAEACPTEAILDPGTATMRVDLGRCTFCGDC
VTACPPGAIRFTGDHRLAARTREALVVSRDAPVLAEALDGELRRVLGRSLKLRQVSAGGCSGCEAELNASGNIQFDVGRF
GVQFVASPRHADGLVITGPVTENMRLALRQTWEAVPAPRIVIAVGACAISGGPFAGSPVTCGGAAAALDGIPVDLYVPGC
PPHPITFLDGILRLLGRLK

Sequences:

>Translated_259_residues
MIDILKFRLGYGNQTVAFPDGPPRFPARFRGRPVVDPSRCEAGCAGCAGCAEACPTEAILDPGTATMRVDLGRCTFCGDC
VTACPPGAIRFTGDHRLAARTREALVVSRDAPVLAEALDGELRRVLGRSLKLRQVSAGGCSGCEAELNASGNIQFDVGRF
GVQFVASPRHADGLVITGPVTENMRLALRQTWEAVPAPRIVIAVGACAISGGPFAGSPVTCGGAAAALDGIPVDLYVPGC
PPHPITFLDGILRLLGRLK
>Mature_259_residues
MIDILKFRLGYGNQTVAFPDGPPRFPARFRGRPVVDPSRCEAGCAGCAGCAEACPTEAILDPGTATMRVDLGRCTFCGDC
VTACPPGAIRFTGDHRLAARTREALVVSRDAPVLAEALDGELRRVLGRSLKLRQVSAGGCSGCEAELNASGNIQFDVGRF
GVQFVASPRHADGLVITGPVTENMRLALRQTWEAVPAPRIVIAVGACAISGGPFAGSPVTCGGAAAALDGIPVDLYVPGC
PPHPITFLDGILRLLGRLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=121, Percent_Identity=38.0165289256198, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1789074, Length=143, Percent_Identity=41.958041958042, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788834, Length=145, Percent_Identity=36.551724137931, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788624, Length=117, Percent_Identity=36.7521367521368, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789075, Length=111, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788617, Length=113, Percent_Identity=33.6283185840708, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17509685, Length=121, Percent_Identity=38.0165289256198, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859983, Length=121, Percent_Identity=38.8429752066116, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24642371, Length=121, Percent_Identity=38.8429752066116, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651058, Length=121, Percent_Identity=38.8429752066116, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 26979; Mature: 26979

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDILKFRLGYGNQTVAFPDGPPRFPARFRGRPVVDPSRCEAGCAGCAGCAEACPTEAIL
CCEEEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCCCHHHHHHHCCCCCEE
DPGTATMRVDLGRCTFCGDCVTACPPGAIRFTGDHRLAARTREALVVSRDAPVLAEALDG
CCCCEEEEEECCCCEECCCHHHHCCCCCEEECCCCHHHHHCCEEEEEECCCCHHHHHHHH
ELRRVLGRSLKLRQVSAGGCSGCEAELNASGNIQFDVGRFGVQFVASPRHADGLVITGPV
HHHHHHCCCCEEEEECCCCCCCCCEEECCCCCEEEECCCCCEEEEECCCCCCCEEEECCC
TENMRLALRQTWEAVPAPRIVIAVGACAISGGPFAGSPVTCGGAAAALDGIPVDLYVPGC
CCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEECCC
PPHPITFLDGILRLLGRLK
CCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDILKFRLGYGNQTVAFPDGPPRFPARFRGRPVVDPSRCEAGCAGCAGCAEACPTEAIL
CCEEEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCCCHHHHHHHCCCCCEE
DPGTATMRVDLGRCTFCGDCVTACPPGAIRFTGDHRLAARTREALVVSRDAPVLAEALDG
CCCCEEEEEECCCCEECCCHHHHCCCCCEEECCCCHHHHHCCEEEEEECCCCHHHHHHHH
ELRRVLGRSLKLRQVSAGGCSGCEAELNASGNIQFDVGRFGVQFVASPRHADGLVITGPV
HHHHHHCCCCEEEEECCCCCCCCCEEECCCCCEEEECCCCCEEEEECCCCCCCEEEECCC
TENMRLALRQTWEAVPAPRIVIAVGACAISGGPFAGSPVTCGGAAAALDGIPVDLYVPGC
CCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEECCC
PPHPITFLDGILRLLGRLK
CCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]