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The map label for this gene is ptsN [H]

Identifier: 220918880

GI number: 220918880

Start: 4214270

End: 4214965

Strand: Direct

Name: ptsN [H]

Synonym: A2cp1_3794

Alternate gene names: 220918880

Gene position: 4214270-4214965 (Clockwise)

Preceding gene: 220918879

Following gene: 220918881

Centisome position: 83.79

GC content: 74.86

Gene sequence:

>696_bases
ATGCAGCTCACCGAACGCGACGCGGCCCGCCTCCTCGGCGTCCCCGAGGCCACCCTGCAGCGGTGGCTGAGGAGCGGCGA
GCTCGCCGCCACCCGGCTGAACGAGCAGTACCGGCTGAACAAGATCGACCTGCTCGAGTTCGCCTCCTCGCGCGGGATGG
AGATCTCGCCCGACCTGCTCGCCGAGCTGGAGCAGCCGGCGCTGCCGAGCCTGGCCGAGGCGATCCGCGCCGGCGGCGTG
CACCACGGGCTGGCCGGCGCCGACAAGTCGGCCACGCTGCGCGACCTGGTGGACCGGCTGGCGCTGCCGCGCGAGGCCGA
CCGCGACCTGGTGCACCGGATGCTGCTCGCGCGCGAGGCGCTCGGCTCCACCGGCGTGGGGAACGGCATCGCCATCCCGC
ACGCGCGGAACCCCATCGTCCTGCACGTGTCCACGCCCGCGGTGGCCATCAGCTACCTCGACCGGCCGGTGGACTTCGAG
GCGCTGGACGGGAAGCCGGTGCACACGCTCGTGCTGCTGGTGAGCCCGTCCACCCGCGCGCACCTGCACCTGCTCGCGCT
GGTGGCCACCGCGCTGCGGGATCCCGGCGTGCTCGCCGCGCTGGACGCGCGCGCCGGAGTGGACGCGCTCGTCCCCGAGA
TCGAGCGGGTGGAGGCCGCCATCGCGGAGAAGCGCGCGGCGGCGAGGAGGCCATGA

Upstream 100 bases:

>100_bases
GTGGCGGTGCTCCGCCTGCCGCTGCTCGCCGGCGTGCTCTCGCGGTCGATCGACGTGGTCCTGGGCATGAAGCGCCTGGG
TTGAAAGGAACCGGAACGGA

Downstream 100 bases:

>100_bases
CGCTCTACCTCGCCGCCGTCGGGGTGCTGGTGGCCGGCGGGCTCGTCGCGCTGGCCGCCTCCGCGCGCCCGCGCCTCGCG
CTCGCCGTGGGCAGCGTCGG

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQLTERDAARLLGVPEATLQRWLRSGELAATRLNEQYRLNKIDLLEFASSRGMEISPDLLAELEQPALPSLAEAIRAGGV
HHGLAGADKSATLRDLVDRLALPREADRDLVHRMLLAREALGSTGVGNGIAIPHARNPIVLHVSTPAVAISYLDRPVDFE
ALDGKPVHTLVLLVSPSTRAHLHLLALVATALRDPGVLAALDARAGVDALVPEIERVEAAIAEKRAAARRP

Sequences:

>Translated_231_residues
MQLTERDAARLLGVPEATLQRWLRSGELAATRLNEQYRLNKIDLLEFASSRGMEISPDLLAELEQPALPSLAEAIRAGGV
HHGLAGADKSATLRDLVDRLALPREADRDLVHRMLLAREALGSTGVGNGIAIPHARNPIVLHVSTPAVAISYLDRPVDFE
ALDGKPVHTLVLLVSPSTRAHLHLLALVATALRDPGVLAALDARAGVDALVPEIERVEAAIAEKRAAARRP
>Mature_231_residues
MQLTERDAARLLGVPEATLQRWLRSGELAATRLNEQYRLNKIDLLEFASSRGMEISPDLLAELEQPALPSLAEAIRAGGV
HHGLAGADKSATLRDLVDRLALPREADRDLVHRMLLAREALGSTGVGNGIAIPHARNPIVLHVSTPAVAISYLDRPVDFE
ALDGKPVHTLVLLVSPSTRAHLHLLALVATALRDPGVLAALDARAGVDALVPEIERVEAAIAEKRAAARRP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=108, Percent_Identity=39.8148148148148, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 24798; Mature: 24798

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTERDAARLLGVPEATLQRWLRSGELAATRLNEQYRLNKIDLLEFASSRGMEISPDLL
CCCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCHHHCCCHHHHHHHHHCCCCCCCHHHH
AELEQPALPSLAEAIRAGGVHHGLAGADKSATLRDLVDRLALPREADRDLVHRMLLAREA
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGSTGVGNGIAIPHARNPIVLHVSTPAVAISYLDRPVDFEALDGKPVHTLVLLVSPSTRA
HCCCCCCCCEECCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHH
HLHLLALVATALRDPGVLAALDARAGVDALVPEIERVEAAIAEKRAAARRP
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQLTERDAARLLGVPEATLQRWLRSGELAATRLNEQYRLNKIDLLEFASSRGMEISPDLL
CCCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCHHHCCCHHHHHHHHHCCCCCCCHHHH
AELEQPALPSLAEAIRAGGVHHGLAGADKSATLRDLVDRLALPREADRDLVHRMLLAREA
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGSTGVGNGIAIPHARNPIVLHVSTPAVAISYLDRPVDFEALDGKPVHTLVLLVSPSTRA
HCCCCCCCCEECCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHH
HLHLLALVATALRDPGVLAALDARAGVDALVPEIERVEAAIAEKRAAARRP
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]