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The map label for this gene is hyfB [H]

Identifier: 220918881

GI number: 220918881

Start: 4214962

End: 4216962

Strand: Direct

Name: hyfB [H]

Synonym: A2cp1_3795

Alternate gene names: 220918881

Gene position: 4214962-4216962 (Clockwise)

Preceding gene: 220918880

Following gene: 220918882

Centisome position: 83.81

GC content: 79.01

Gene sequence:

>2001_bases
ATGACGCTCTACCTCGCCGCCGTCGGGGTGCTGGTGGCCGGCGGGCTCGTCGCGCTGGCCGCCTCCGCGCGCCCGCGCCT
CGCGCTCGCCGTGGGCAGCGTCGGCGCCGCGCTGGGCTGCGCGCTCGGGCTCGCCGCCTCGCTCGCCGCGCTGCGCGCCC
CGCCCGCCGCGCTCGACCTCTCCTGGCCGGTGCCCTCCGGGGCGCTCGCGCTCGGCCTCGACCCGCTCTCGGCCGCGTTC
GCGACCGCGATCTTCGCGCTCGGCCTCGCCGCCTCGGTGTACGGCGCCGGCTACCTGCGCCCGCAGGCCGACCGCCGCTC
CATGGGGCCGTTCGCGCTGTTCTTCGCGCTCACCCTCGCGAGCATGGCGCTCGTGGTCGCGGCCCGGCAGGCGGTGCTGT
TCCTGGTGGCGTGGGAGGGCATGACCGCCGCGTCCTTCCTGCTCGTCGCCCACGAGCACGAGGACGCCTCGGTGCGCCGC
GCCGCCCGCACCTACCTCGTGGCCTCGCACCTCGGCGCCGCGTTCCTGTTCGCCCTGTTCCTGGTGCTCGGGCGCGAGGC
GGGCTCGCTCCGCTTCGAGGCGTTCGCCGCGCTGCGGGCCGGCGGCGCGCCCGCGGCGCTCCTGGTCCTGTTCGCGCTGG
TGGGCTTCGGCACCAAGGCCGGCCTGGTGCCGCTGCACGTCTGGCTGCCCGAGGCCCACCCGGCCGCGCCGAGCCACGTG
TCCGCGCTCATGTCCGGCGTGCTCGTGAAGATGGGCGTGTACGGCATCCTGCGGGTGCTCACGTTCCTGCCGCCCGCGCC
GGCGTGGGCCGGGCTGCTGCTCGCCGGGGTGGGCCTGGTGGGCGCGCTCGCGGCGCTGGCGCTCGCGCTCGGGCAGCGCG
ACCTGAAGCGCGTGCTCGCGTACTCCACCGTCGAGAACGTGGGGATCATCGCGCTCGCCGCCGGCCTGGGGCTCGTCGGG
GCCGCGGTCCACGCGCCGCTGGTGGCGGCGCTCGGCGTCACCGCCGCGCTGCTCCACGTCTGGAACCACGCGCTCATGAA
GGGGCTCGCGTTCCTGGGCGCCGGGGCGCTGGTGCACGGCGCGGGCACGCGCGACCTGGAGCGGATGGGCGGGCTGCTCC
GGCGGCTGCCCGTCACCGGCGGCCTGCTGGTGCTCGCCGCGGTGGCGCTGTGCGGGCTGCCGCCGCTGAACGGGTTCGTC
TCGGAGTGGCTCGTGTACCTGGGCCTGCTGCAGGGCGGGCGCGGCGCGCCGGGCGGCCTGGCGCTGGCGGCGTGGCTGGC
GCTCGCCGCGCTCGCGCTGGTGGGCGCGCTCGCGGCGGTGGTGTTCACGCGGCTCCTCGGCACCGCGCTCCTGGGCGAGC
CGCGCAGCGAGGAGGCCGCGCACGCGCACGAGGCCGGGCCGCTCCTGCTCGCGCCGCTCGCGCTGCTCGGGGCGGGCAAC
GTGGCCCTGGCGCTGTTCCCGGCGCACGCGGCCGCGCTGCTCGCGCCCGCCGCGGCGCAGGTCCTCGGGACCACCGAGGG
CGAGGTGCTCGCCGCGCTCGCGCCCGCCGCCGGCGCGCTGGCCGGCCCGCTGCGGGCGGGCGTCCTCGTGCTGGTGGCGC
TCGGGGTCGGCGTCGCGATCGCCGCGCGGCGGCTGCGGGCGGGCCGGCCGGTGGCCGAGTCCTCGACCTGGGGCTGCGGC
TTCTCCGCCGCGAGCCCGCGCGTCCAGTACACCGCCGCCTCCTACGCGCAGCTCGCGCTCGACGCCGCCGTGCCGCGCCC
GCTCCGGCCGCGCGCGCGCCTGCGGCCGCCGGAGGGCGCGTTCCCGGCCGGCGCGTCGCTCGCGCTGGAGGGCGACGACC
CGGCGCGCACCCGCGTGTTCGAGCCGGCGTTCCAGCGGGTGGGCGGCTGGTTCAGCCGGCTGCGCAGCTTCCAGCAGGCC
CGCCTCAACCTCCAGCTCCTCTACACCGTCGCGGCGCTGCTCGGGCTCACGGCGCTGCTGCTCCTCCGGTACGGTCCGTG
A

Upstream 100 bases:

>100_bases
TCGCCGCGCTGGACGCGCGCGCCGGAGTGGACGCGCTCGTCCCCGAGATCGAGCGGGTGGAGGCCGCCATCGCGGAGAAG
CGCGCGGCGGCGAGGAGGCC

Downstream 100 bases:

>100_bases
CCTCCGCGCTCGCCCTCGGCGCCATCGCCCTCACCGCCGCGAGCGGCCTCGCCGCGGCGCTGCCCGGCCTCCGCCCCGCG
CGCGCGCAGGCGCTCGCCAC

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MTLYLAAVGVLVAGGLVALAASARPRLALAVGSVGAALGCALGLAASLAALRAPPAALDLSWPVPSGALALGLDPLSAAF
ATAIFALGLAASVYGAGYLRPQADRRSMGPFALFFALTLASMALVVAARQAVLFLVAWEGMTAASFLLVAHEHEDASVRR
AARTYLVASHLGAAFLFALFLVLGREAGSLRFEAFAALRAGGAPAALLVLFALVGFGTKAGLVPLHVWLPEAHPAAPSHV
SALMSGVLVKMGVYGILRVLTFLPPAPAWAGLLLAGVGLVGALAALALALGQRDLKRVLAYSTVENVGIIALAAGLGLVG
AAVHAPLVAALGVTAALLHVWNHALMKGLAFLGAGALVHGAGTRDLERMGGLLRRLPVTGGLLVLAAVALCGLPPLNGFV
SEWLVYLGLLQGGRGAPGGLALAAWLALAALALVGALAAVVFTRLLGTALLGEPRSEEAAHAHEAGPLLLAPLALLGAGN
VALALFPAHAAALLAPAAAQVLGTTEGEVLAALAPAAGALAGPLRAGVLVLVALGVGVAIAARRLRAGRPVAESSTWGCG
FSAASPRVQYTAASYAQLALDAAVPRPLRPRARLRPPEGAFPAGASLALEGDDPARTRVFEPAFQRVGGWFSRLRSFQQA
RLNLQLLYTVAALLGLTALLLLRYGP

Sequences:

>Translated_666_residues
MTLYLAAVGVLVAGGLVALAASARPRLALAVGSVGAALGCALGLAASLAALRAPPAALDLSWPVPSGALALGLDPLSAAF
ATAIFALGLAASVYGAGYLRPQADRRSMGPFALFFALTLASMALVVAARQAVLFLVAWEGMTAASFLLVAHEHEDASVRR
AARTYLVASHLGAAFLFALFLVLGREAGSLRFEAFAALRAGGAPAALLVLFALVGFGTKAGLVPLHVWLPEAHPAAPSHV
SALMSGVLVKMGVYGILRVLTFLPPAPAWAGLLLAGVGLVGALAALALALGQRDLKRVLAYSTVENVGIIALAAGLGLVG
AAVHAPLVAALGVTAALLHVWNHALMKGLAFLGAGALVHGAGTRDLERMGGLLRRLPVTGGLLVLAAVALCGLPPLNGFV
SEWLVYLGLLQGGRGAPGGLALAAWLALAALALVGALAAVVFTRLLGTALLGEPRSEEAAHAHEAGPLLLAPLALLGAGN
VALALFPAHAAALLAPAAAQVLGTTEGEVLAALAPAAGALAGPLRAGVLVLVALGVGVAIAARRLRAGRPVAESSTWGCG
FSAASPRVQYTAASYAQLALDAAVPRPLRPRARLRPPEGAFPAGASLALEGDDPARTRVFEPAFQRVGGWFSRLRSFQQA
RLNLQLLYTVAALLGLTALLLLRYGP
>Mature_665_residues
TLYLAAVGVLVAGGLVALAASARPRLALAVGSVGAALGCALGLAASLAALRAPPAALDLSWPVPSGALALGLDPLSAAFA
TAIFALGLAASVYGAGYLRPQADRRSMGPFALFFALTLASMALVVAARQAVLFLVAWEGMTAASFLLVAHEHEDASVRRA
ARTYLVASHLGAAFLFALFLVLGREAGSLRFEAFAALRAGGAPAALLVLFALVGFGTKAGLVPLHVWLPEAHPAAPSHVS
ALMSGVLVKMGVYGILRVLTFLPPAPAWAGLLLAGVGLVGALAALALALGQRDLKRVLAYSTVENVGIIALAAGLGLVGA
AVHAPLVAALGVTAALLHVWNHALMKGLAFLGAGALVHGAGTRDLERMGGLLRRLPVTGGLLVLAAVALCGLPPLNGFVS
EWLVYLGLLQGGRGAPGGLALAAWLALAALALVGALAAVVFTRLLGTALLGEPRSEEAAHAHEAGPLLLAPLALLGAGNV
ALALFPAHAAALLAPAAAQVLGTTEGEVLAALAPAAGALAGPLRAGVLVLVALGVGVAIAARRLRAGRPVAESSTWGCGF
SAASPRVQYTAASYAQLALDAAVPRPLRPRARLRPPEGAFPAGASLALEGDDPARTRVFEPAFQRVGGWFSRLRSFQQAR
LNLQLLYTVAALLGLTALLLLRYGP

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=418, Percent_Identity=26.7942583732057, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1788827, Length=442, Percent_Identity=39.8190045248869, Blast_Score=299, Evalue=5e-82,
Organism=Escherichia coli, GI1788831, Length=353, Percent_Identity=35.6940509915014, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI2367154, Length=540, Percent_Identity=31.1111111111111, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788829, Length=405, Percent_Identity=29.6296296296296, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1788614, Length=410, Percent_Identity=30.7317073170732, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788613, Length=318, Percent_Identity=29.2452830188679, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 67192; Mature: 67061

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLYLAAVGVLVAGGLVALAASARPRLALAVGSVGAALGCALGLAASLAALRAPPAALDL
CEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
SWPVPSGALALGLDPLSAAFATAIFALGLAASVYGAGYLRPQADRRSMGPFALFFALTLA
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
SMALVVAARQAVLFLVAWEGMTAASFLLVAHEHEDASVRRAARTYLVASHLGAAFLFALF
HHHHHHHHHHHHHEEEEECCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVLGREAGSLRFEAFAALRAGGAPAALLVLFALVGFGTKAGLVPLHVWLPEAHPAAPSHV
HHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHH
SALMSGVLVKMGVYGILRVLTFLPPAPAWAGLLLAGVGLVGALAALALALGQRDLKRVLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSTVENVGIIALAAGLGLVGAAVHAPLVAALGVTAALLHVWNHALMKGLAFLGAGALVHG
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEC
AGTRDLERMGGLLRRLPVTGGLLVLAAVALCGLPPLNGFVSEWLVYLGLLQGGRGAPGGL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
ALAAWLALAALALVGALAAVVFTRLLGTALLGEPRSEEAAHAHEAGPLLLAPLALLGAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCCCC
VALALFPAHAAALLAPAAAQVLGTTEGEVLAALAPAAGALAGPLRAGVLVLVALGVGVAI
CEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AARRLRAGRPVAESSTWGCGFSAASPRVQYTAASYAQLALDAAVPRPLRPRARLRPPEGA
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FPAGASLALEGDDPARTRVFEPAFQRVGGWFSRLRSFQQARLNLQLLYTVAALLGLTALL
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRYGP
HHHCCC
>Mature Secondary Structure 
TLYLAAVGVLVAGGLVALAASARPRLALAVGSVGAALGCALGLAASLAALRAPPAALDL
EEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
SWPVPSGALALGLDPLSAAFATAIFALGLAASVYGAGYLRPQADRRSMGPFALFFALTLA
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
SMALVVAARQAVLFLVAWEGMTAASFLLVAHEHEDASVRRAARTYLVASHLGAAFLFALF
HHHHHHHHHHHHHEEEEECCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVLGREAGSLRFEAFAALRAGGAPAALLVLFALVGFGTKAGLVPLHVWLPEAHPAAPSHV
HHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHH
SALMSGVLVKMGVYGILRVLTFLPPAPAWAGLLLAGVGLVGALAALALALGQRDLKRVLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSTVENVGIIALAAGLGLVGAAVHAPLVAALGVTAALLHVWNHALMKGLAFLGAGALVHG
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEC
AGTRDLERMGGLLRRLPVTGGLLVLAAVALCGLPPLNGFVSEWLVYLGLLQGGRGAPGGL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
ALAAWLALAALALVGALAAVVFTRLLGTALLGEPRSEEAAHAHEAGPLLLAPLALLGAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHCCCC
VALALFPAHAAALLAPAAAQVLGTTEGEVLAALAPAAGALAGPLRAGVLVLVALGVGVAI
CEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AARRLRAGRPVAESSTWGCGFSAASPRVQYTAASYAQLALDAAVPRPLRPRARLRPPEGA
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FPAGASLALEGDDPARTRVFEPAFQRVGGWFSRLRSFQQARLNLQLLYTVAALLGLTALL
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRYGP
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]